Meishan Yan, Zelong Wang, Yao An, Zhanni Li, Yun Li, Hongyu Zhang, Caixia Li, Lifeng Wang, Li Chen, Chao Gao, Dongsheng Wang, Chunyan Gao
Oxidized low-density lipoprotein (oxLDL), a key component in atherosclerosis and hyperlipidemia, is a risk factor for atherothrombosis in dyslipidemia, yet its mechanism is poorly understood. In this study, we used oxLDL-induced human aortic endothelial cells (HAECs) and high-fat diet (HFD)-fed mice as a hyperlipidemia model. Phosphatidylserine (PS) exposure, cytosolic Ca2+ , reactive oxygen species (ROS), and lipid peroxidation were measured by flow cytometer. TMEM16F expression was detected by immunofluorescence, western blot, and reverse transcription polymerase chain reaction...
March 5, 2024: Cell Biology International