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Giacomo Foli, Micaela Degli Esposti, Maurizio Toselli, Davide Morselli, Paola Fabbri
A new, facile and low-sample consuming technique for the determination of hydroxyl values (OHVs) in poly(hydroxyalkanoate)s (PHAs) is herein presented. After a fast, uncatalyzed and non-destructive trifluoroacetylation of all PHA hydroxyl groups, OHVs are calculated through 19F-NMR measurements, comparing integral values of the only two signals in the spectra: the fluorinated hydroxyl groups and the internal standard (trifluorotoluene) signals. Furthermore, the combined ex situ functionalization with a simple workup of the fluorinated polymer allowed to obtain spectra with an improved signal/noise ratio and short acquisition time if compared to 1H-NMR-based methods...
February 8, 2019: Analyst
Guodong Gu, Michelle Black, Colt Cookson, Anna Fiorella, Yinghe Li, Steven H Gorman, Ray Bakhtiar
A sensitive, selective, and reliable LC-MS/MS method was developed and validated for simultaneous quantification of venlafaxine (VEN) and its 5 metabolites (ODV, NDV, NNDDV, OHV and NODDV) in rat plasma. The calibration ranges are 15.0 to 6000 ng/mL for VEN, 1.00 to 400 ng/mL for ODV, 5.00 to 2000 ng/mL for NDV, 1.00 to 400 ng/mL for NNDDV, 10.0 to 4000 ng/mL for OHV, and 0.200 to 20.0 ng/mL for NODDV. Briefly, 50 μL of rat plasma was extracted using liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE)...
June 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Dominik Müller, Pascal Schopp, Albrecht E Melchinger
Genomic selection (GS) offers the possibility to estimate the effects of genome-wide molecular markers, which can be used to calculate genomic estimated breeding values (GEBVs) for individuals without phenotypes. GEBVs can serve as a selection criterion in recurrent GS, maximizing single-cycle but not necessarily long-term genetic gain. As simple genome-wide sums, GEBVs do not take into account other genomic information, such as the map positions of loci and linkage phases of alleles. Therefore, we herein propose a novel selection criterion called expected maximum haploid breeding value (EMBV)...
March 28, 2018: G3: Genes—Genomes—Genetics
Matthew Goiffon, Aaron Kusmec, Lizhi Wang, Guiping Hu, Patrick S Schnable
Genomic selection (GS) identifies individuals for inclusion in breeding programs based on the sum of their estimated marker effects or genomic estimated breeding values (GEBVs). Due to significant correlation between GEBVs and true breeding values, this has resulted in enhanced rates of genetic gain as compared to traditional methods of selection. Three extensions to GS, weighted genomic selection (WGS), optimal haploid value (OHV) selection, and genotype building (GB) selection have been proposed to improve long-term response, and to facilitate the efficient development of doubled haploids...
July 2017: Genetics
Nathan A Custer, Lesley A DeFalco, Kenneth E Nussear, Todd C Esque
Public land policies manage multiple uses while striving to protect vulnerable plant and wildlife habitats from degradation; yet the effectiveness of such policies are infrequently evaluated, particularly for remote landscapes that are difficult to monitor. We assessed the use and impacts of recreational vehicles on Mojave Desert washes (intermittent streams) in the Chemehuevi Desert Wildlife Management Area (DWMA) of southern California. Wash zones designated as open and closed to off-highway vehicle (OHV) activity were designed in part to protect Mojave desert tortoise (Gopherus agassizii) habitat while allowing recreation in designated areas...
May 15, 2017: Journal of Environmental Management
Jonathan R Weir-McCall, Anu Kamalasanan, Deidre B Cassidy, Allan D Struthers, Brian J Lipworth, J Graeme Houston
INTRODUCTION: To compare the reproducibility of pulmonary pulse wave velocity (PWV) techniques, and the effects of age and exercise on these. METHODS: 10 young healthy volunteers (YHV) and 20 older healthy volunteers (OHV) with no cardiac or lung condition were recruited. High temporal resolution phase contrast sequences were performed through the main pulmonary arteries (MPAs), right pulmonary arteries (RPAs) and left pulmonary arteries (LPAs), while high spatial resolution sequences were obtained through the MPA...
2016: BMJ Open Respiratory Research
Maria E Linnaus, Rebecca L Ragar, Erin M Garvey, Jason D Fraser
PURPOSE: To identify injuries and outcomes from Recreational/Off-Highway Vehicles (RV/OHV) accidents at a pediatric trauma center. METHODS: A retrospective review of a prospective pediatric trauma registry was performed to identify patients sustaining injuries from an RV/OHV between January 2007 and July 2015. Vehicles included: all-terrain vehicles (ATV), dirt bikes, utility-terrain vehicles (UTV), golf carts, go-karts, and dune buggies. RESULTS: Five hundred twenty-eight patients were injured while on an RV/OHV: 269 ATV, 135 dirt bike, 42 UTV, 38 golf cart, 34 go-kart, and 10 dune buggy...
February 2017: Journal of Pediatric Surgery
A L Graham, D H Nussey, J O Lloyd-Smith, D Longbottom, M Maley, J M Pemberton, J G Pilkington, K C Prager, L Smith, K A Watt, K Wilson, T N McNEILLY, F Brülisauer
We assessed evidence of exposure to viruses and bacteria in an unmanaged and long-isolated population of Soay sheep (Ovis aries) inhabiting Hirta, in the St Kilda archipelago, 65 km west of Benbecula in the Outer Hebrides of Scotland. The sheep harbour many metazoan and protozoan parasites but their exposure to viral and bacterial pathogens is unknown. We tested for herpes viral DNA in leucocytes and found that 21 of 42 tested sheep were infected with ovine herpesvirus 2 (OHV-2). We also tested 750 plasma samples collected between 1997 and 2010 for evidence of exposure to seven other viral and bacterial agents common in domestic Scottish sheep...
July 2016: Epidemiology and Infection
Hans D Daetwyler, Matthew J Hayden, German C Spangenberg, Ben J Hayes
Doubled haploids are routinely created and phenotypically selected in plant breeding programs to accelerate the breeding cycle. Genomic selection, which makes use of both phenotypes and genotypes, has been shown to further improve genetic gain through prediction of performance before or without phenotypic characterization of novel germplasm. Additional opportunities exist to combine genomic prediction methods with the creation of doubled haploids. Here we propose an extension to genomic selection, optimal haploid value (OHV) selection, which predicts the best doubled haploid that can be produced from a segregating plant...
August 2015: Genetics
José Enrique Muñoz de Escalona-Rojas, Jesús Cantero-Hinojosa, Fermín Garrido-Pareja, José Luis García-Serrano, José Francisco Padilla-Torres
BACKGROUND AND OBJECTIVE: We sought to study the association of glaucoma with vascular disease, with 2 independent pathways: the association of glaucoma with cardiovascular disease (CVD) and the study of ocular hemodynamic variables (OHV) in glaucoma. MATERIAL AND METHOD: Cross-sectional study consisting of 73 patients: 25 without glaucoma, 28 primary open-angle glaucoma (POAG) and 20 normal-tension glaucoma (NTG). OHV, cardiovascular risk factors (CVRF) and CVD were determined...
August 19, 2014: Medicina Clínica
Hui-Ching Chang, Tein-Shun Tsai, Inn-Ho Tsai
UNLABELLED: This study deciphers the geographic variations of king cobra (Ophiophagus hannah) venom using functional proteomics. Pooled samples of king cobra venom (abbreviated as Ohv) were obtained from Indonesia, Malaysia, Thailand, and two provinces of China, namely Guangxi and Hainan. Using two animal models to test and compare the lethal effects, we found that the Chinese Ohvs were more fatal to mice, while the Southeast Asian Ohvs were more fatal to lizards (Eutropis multifasciata)...
August 26, 2013: Journal of Proteomics
Gerene M Denning, Charles A Jennissen, Karisa K Harland, David G Ellis, Christopher T Buresh
The number of off-highway vehicle (OHV) parks continues to grow to meet the recreational needs of ATV enthusiasts and the increasing popularity of the vehicle. Little is known about how OHV park regulations and enforcement affect ATV safety among their users. This study was designed to determine whether there were differences in crash mechanisms and/or compliance with ATV safety laws and regulations when comparing off-road ATV crashes inside and outside state OHV parks. Relative to outside the parks, a smaller percentage of park victims were under the age of sixteen, a lower percentage were passengers, and a dramatically higher percentage were helmeted...
March 2013: Accident; Analysis and Prevention
Namyun Kil, Stephen M Holland, Taylor V Stein
Off-highway vehicle (OHV) riding is among the fastest growing recreational activities in the United States. However, little research exists about the central components of outcomes-focused management (OFM) as it relates to motorized recreation. Utilizing a two-activity dichotomy, OHV and non-OHV centric user groups were compared on several key concepts associated with OFM, including desired experiences, perceived and desired recreation opportunity spectrum-type settings, and intentional behaviors (i.e., place-protective behavior, spending-time intentions) toward potential changes in settings...
September 2012: Environmental Management
Glyn Bissix, Keith MacCormick, Chris Milburn
Recently, a study by Burr and his associates culminating in three peer reviewed journal articles and a string of press releases emanating from the off-highway vehicle (OHV) industry claimed that increasing riding time on all terrain recreational vehicles (ATVs) and off road motorcycles (ORMs) could meet the basic guidelines of the American College of Sports Medicine and Health Canada for sufficient physical activity leading to positive health adaptations. Should this be true, this study could revolutionize the way that health professionals prescribe physical activity...
March 2013: Health Promotion International
R Loginov, T Karlsson, K Höckerstedt, D Ablashi, I Lautenschlager
Human herpesvirus-6 (HHV-6) infection, mostly caused by variant B, is common after transplantation. Here, we report a new modified method using an HHV-6B glycoprotein IgG antibody, OHV-3, and attempt to quantify the HHV-6 antigenemia after liver transplantation. Twenty-four liver transplant recipients were frequently monitored by the HHV-6 antigenemia test, which detects the HHV-6B virion protein in peripheral blood mononuclear cells (PBMC). HHV-6B antigens were now retrospectively demonstrated using a glycoprotein OHV-3 IgG antibody in the immunoperoxidase staining from the same specimens and quantified as positive cells/10,000 PBMC...
July 2010: European Journal of Clinical Microbiology & Infectious Diseases
Daniel Deisenroth, John Loomis, Craig Bond
Few economic studies are available to measure off-highway vehicle recreation benefits foregone when trails must be closed to protect the environment. This paper estimates the non-market benefits associated with off-highway vehicle (OHV) recreation on National Forest lands in Larimer County, Colorado. We use a contingent valuation model (CVM) to estimate benefits to OHV users, which includes dirt bike riders, all terrain vehicle (ATV) riders, and 4-wheel drive (4x4) users. Using CVM we find the mean consumer surplus estimates to be $78 per person per day...
August 2009: Journal of Environmental Management
Aleksija S Neimanis, Janet E Hill, Claire M Jardine, Trent K Bollinger
Malignant catarrhal fever (MCF) is a sporadic disease of artiodactyls caused by several viruses in the Gammaherpesvirinae. We report two cases of MCF in free-living moose (Alces alces) from Saskatchewan. One was a thin, dehydrated, adult male found recumbent in 2006. At necropsy, ulcers were found in the intestine, bladder, and corneas. Microscopically, there was lymphocytic vasculitis and perivasculitis in many organs with infrequent fibrinoid necrosis. Ovine herpes virus-2 (OHV-2) was identified by polymerase chain reaction...
January 2009: Journal of Wildlife Diseases
Keith Cole, Matt Kruger, Dylan Bates, Greg Steil, Mike Zbreski
BACKGROUND CONTEXT: Pain and disability after lumbar fusion surgery contributes to the over $20 billion dollars spent in health-care costs and estimated $28 billion in lost wages annually. With the goal of returning to work, an intensive program designed to build functional strength may be used. Previous interventions for this subgroup report the outcome measure of return to work (RTW), but do not account for the physical demand of the job to which they are returning. This may account for varying RTW and re-injury rates...
January 2009: Spine Journal: Official Journal of the North American Spine Society
J S Munday, A F French, A Smith, J Wang, R A Squires
CASE HISTORY: A 2-year-old crossbred cow developed crusting ulcerative lesions that covered approximately 40% of the body. They were first observed 2 weeks after the cow calved, and were most severe over the caudal aspect of the proximal hindlegs and perineum. CLINICAL FINDINGS AND DIAGNOSIS: Generalised variably confluent 1-2-cm diameter foci of ulceration and crusting were visible. No ocular or oral lesions were visible, and the cow did not have diarrhoea. Skin biopsies revealed lesions consistent with those previously described for malignant catarrhal fever (MCF)...
April 2008: New Zealand Veterinary Journal
Timothy M D'Andrea, Xu Zhang, Evan B Jochnowitz, T G Lindeman, C J S M Simpson, Donald E David, Thomas J Curtiss, John R Morris, G Barney Ellison
We have studied the oxidation of self-assembled monolayers (SAMs) of alkanes and alkenes with a thermal beam of OH radicals. The target films were produced by bonding alkane thiols and alkene thiols to a gold surface and the SAMs are mounted in a vacuum chamber at a base pressure of 10-9 Torr. Hydroxyl radicals were produced by a corona discharge in an Ar/H2O2/water mixture. The resultant molecular beam was scanned by an electrostatic hexapole and the OH radicals [4 (+/- 1) x 1011 OH radicals cm-2 sec-1] were focused onto the target SAM...
January 17, 2008: Journal of Physical Chemistry. B
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