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bioanalytical chemistry

Marianna Rossetti, Erica Del Grosso, Simona Ranallo, Davide Mariottini, Andrea Idili, Alessandro Bertucci, Alessandro Porchetta
The emerging field of RNA nanotechnology harnesses the versatility of RNA molecules to generate nature-inspired systems with programmable structure and functionality. Such methodology has therefore gained appeal in the fields of biosensing and diagnostics, where specific molecular recognition and advanced input/output processing are demanded. The use of RNA modules and components allows for achieving diversity in structure and function, for processing information with molecular precision, and for programming dynamic operations on the grounds of predictable non-covalent interactions...
February 8, 2019: Analytical and Bioanalytical Chemistry
Alain Beck, Valentina D'Atri, Anthony Ehkirch, Szabolcs Fekete, Oscar Hernandez-Alba, Rabah Gahoual, Emmanuel Leize-Wagner, Yannis François, Davy Guillarme, Sarah Cianférani
The development and optimization of antibody drug conjugates (ADCs) rely on improving their analytical and bioanalytical characterization, by assessing critical quality attributes (CQAs). Among the CQAs, the glycoprofile, drug load distribution (DLD), the amount of unconjugated antibody (D0), the average drug-to-antibody ratio (DAR), the drug conjugation sites and the residual drug-linker and related product proportions (SMDs) in addition to high and low molecular weight species (H/LMWS), and charge variants are the most important ones...
February 1, 2019: Expert Review of Proteomics
Mukul Sonker, Daihyun Kim, Ana Egatz-Gomez, Alexandra Ros
Separations of bioanalytes require robust, effective, and selective migration phenomena. However, due to the complexity of biological matrices such as body fluids or tissue, these requirements are difficult to achieve. The separations field is thus constantly evolving to develop suitable methods to separate biomarkers and fractionate biospecimens for further interrogation of biomolecular content. Advances in the field of microfabrication allow the tailored generation of micro- and nanofluidic environments. These can be exploited to induce interactions and dynamics of biological species with the corresponding geometrical features, which in turn can be capitalized for novel separation approaches...
January 30, 2019: Annual Review of Analytical Chemistry
Rafael Venson, Ann-Sophie Korb, Gail Cooper
Over the past three decades, many studies employing hollow-fiber liquid-phase microextraction (HF-LPME) bioanalytical methods have been published. The basic mechanism of extraction relies on the migration of the analytes through a liquid membrane sustained in the pores of the walls of a porous hollow fiber, and from there into an acceptor phase present in the lumen of the fiber. The mass transfer occurs by passive diffusion and it can be enhanced by using a carrier or applying an electrical potential across the phases...
January 17, 2019: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Gabriela de Oliveira Silveira, Ana Miguel Fonseca Pego, Jefferson Pereira E Silva, Mauricio Yonamine
The sample preparation is the most critical step involved in the bioanalytical process. When dealing with green analytical chemistry, sample preparation can be even more challenging. To fit the green analytical chemistry principles, efforts should be made toward the elimination or reduction of the use of toxic reagents and solvents, minimization of energy consumption and increased operator safety. The simplest sample preparations are more appropriate for liquid biological matrices with little interfering compounds such as urine, plasma and oral fluid...
January 21, 2019: Bioanalysis
Charles Chatard, Andrei Sabac, Laura Moreno-Velasquez, Anne Meiller, Stephane Marinesco
The ability to monitor the chemical composition of brain interstitial fluid remains an important challenge in the field of bioanalytical chemistry. In particular, microelectrode biosensors are a promising resource for the detection of neurochemicals in interstitial fluid in both animals and humans. These biosensors can provide second-by-second temporal resolution and enzymatic recognition of virtually any redox or nonredox molecule. However, despite miniaturization of these sensors to 50-250 μm in diameter to avoid vascular and cellular injury, inflammation and foreign-body reactions still occur following their implantation...
December 26, 2018: ACS Central Science
Rodrigo Brito Santos, Railmara Pereira da Silva, Felipe Akihiro Melo Otsuka, Danielle de Jessus Trindade, Aline Costa Santos, Humberto Reis Matos
Hexokinases play a critical role in the cellular uptake and utilization of glucose. As such, they are of fundamental importance to all cells. By catalyzing of glucose to produce glucose-6-phosphate, hexokinases control the first irreversible step of glucose metabolism and initiating all major pathways of glucose consumption. Our objective was to develop and validate highly sensitive and selective high performance liquid chromatography with photodiode array detector (HPLC-PDA) assays allowing the determination of adenosine diphosphate (ADP), which was used for determination of the hexokinase activity...
December 19, 2018: Biomedical Chromatography: BMC
Peng Li, Tony Jun Huang
Acoustics has a broad spectrum of applications, ranging from noise cancelation to ultrasonic imaging. In the past decade, there has been increasing interest in developing acoustic-based methods for biological and biomedical applications. This Perspective summarizes the recent progress in applying acoustofluidic methods (i.e., the fusion of acoustics and microfluidics) to bioanalytical chemistry. We describe the concepts of acoustofluidics and how it can be tailored to different types of bioanalytical applications, including sample concentration, fluorescence-activated cell sorting, label-free cell/particle separation, and fluid manipulation...
December 18, 2018: Analytical Chemistry
Sharmistha Chatterjee, Loredana Ricciardi, Julia I Deitz, Robert E A Williams, David W McComb, Giuseppe Strangi
We report the study of heterodimeric plasmonic nanogaps created between gold nanostar (AuNS) tips and gold nanospheres. The selective binding is realized by properly functionalizing the two nanostructures; in particular, the hot electrons injected at the nanostar tips trigger a regio-specific chemical link with the functionalized nanospheres. AuNSs were synthesized in a simple, one-step, surfactant-free, high-yield wet-chemistry method. The high aspect ratio of the sharp nanostar tip collects and concentrates intense electromagnetic fields in ultrasmall surfaces with small curvature radius...
December 16, 2018: Micromachines
Mingtai Sun, Xin Yang, Yuannian Zhang, Suhua Wang, Ming Wah Wong, Runyan Ni, Dejian Huang
Herein we report on fluorophore-tagged Grubbs catalysts as turn-on fluorescent probes for the sensitive detection and quantitation of ethylene, a plant hormone that plays a critical role in many phases of plant growth and fruit ripening. The ruthenium (Ru) based weakly fluorescent probes were prepared handily through metathesis reaction between the first generation Grubbs catalyst and selected fluorophores that have high quantum yields and contain terminal vinyl groups. Upon exposure to ethylene, fluorescence enhancement was observed via the release of fluorophore from the probe...
December 3, 2018: Journal of Agricultural and Food Chemistry
Ke Yang, Huizhen Wang, Ning Ma, Ming Zeng, Huaiqing Luo, Dinggeng He
Synthetic DNA machines that operate on the nanoscale three-dimensional (3D) track have attracted rapidly increasing interest because of their potential in biocomputing, drug delivery, and biosensing applications. Current nanoscale 3D DNA tracks are typically created by self-assembling thiolated oligonucleotides at gold nanoparticles (AuNPs) surface via the strong Au-S chemistry. However, it remains challenging to precisely control the orientation and conformation of 3D DNA track on AuNPs surface and finely tune the hybridization ability of 3D track...
November 29, 2018: ACS Applied Materials & Interfaces
Mohammad Pousti, Maxime Joly, Patrice Roberge, Mehran Abbaszadeh Amirdehi, Andre Begin-Drolet, Jesse Greener
A fully automated linear scanning attenuated total reflection (ATR) accessory is presented for Fourier transform infrared (FTIR) spectrosco-py. The approach is based on the accurate displacement of a multi-bounce ATR crystal relative to a stationary infrared beam. To ensure accu-rate positioning and to provide a second sample characterization mode, a custom-built microscope was integrated into the system and the computerized work flow. Custom software includes automated control and measurement routines with a straightforward user interface for selecting parameters and monitoring experimental progress...
November 18, 2018: Analytical Chemistry
Evangelia G Chronopoulou, Nikolaos E Labrou
Glutathione transferases (GSTs) catalyze the conjugation of glutathione (GSH) to endogenous and xenobiotic electrophilic compounds and have been involved in the development of resistance toward cancer chemotherapeutic drugs and in the etiology, pathology and progression of several other diseases. In the present work, the human isoenzyme GSTA1-1 (hGSTA1-1) was used to assemble a microplate-based platform for high-throughput screening of inhibitors from plant extracts. The enzyme was immobilized using sol-gel chemistry and deposited as a layer at the bottom surface of 96-well format ELISA microplate...
October 28, 2018: Current Pharmaceutical Biotechnology
Selvakumar Palanisamy, Vijayalakshmi Velusamy, Shih-Wen Chen, Thomas C K Yang, Sridharan Balu, Craig E Banks
In recent years, the carbohydrate polymers incorporated composite materials have shown significant interest in the bioanalytical chemistry due to their enhanced catalytic performances of various enzymes or mimics. This paper reports the fabrication of novel H2 O2 biosensor using a hemin immobilized reduced graphene oxide-cellulose microfiber composite (hemin/RGO-CMF). The RGO-CMF composite was prepared by the reduction of GO-CMF composite using vitamin C as a reducing agent. Various physio-chemical methods have applied for the characterization of RGO-CMF composite...
January 15, 2019: Carbohydrate Polymers
Pradip Das, Abootaleb Sedighi, Ulrich J Krull
The development of bioanalytical methods that provide early detection of the presence of cancer by sensitive and specific determination of biomarkers such as small biomolecules, nucleic acids, proteins, enzymes, and even whole cells are essential to improve opportunity for improved patient treatment and to diminish the rate of cancer mortality. Förster resonance energy transfer (FRET) methods have been increasingly used to develop bioassays that offer speed, selectivity and low detection levels with practicality that is appropriate for providing point-of-care measurements for screening...
December 24, 2018: Analytica Chimica Acta
Guanyue Gao, Dengchao Wang, Ricardo Brocenschi, Jinfang Zhi, Michael V Mirkin
Fast and cost-efficient detection and identification of bacteria in food and water samples and biological fluids is an important challenge in bioanalytical chemistry. It was shown recently that bacteria can be detected by measuring the decrease in the diffusion current to the ultramicroelectrode caused by cell collisions with its surface. To add selectivity to the bacteria detection, herein we show the possibility of collision experiments with the signal produced by electrochemical activity of bacterial cells reducing (or oxidizing) redox species...
September 27, 2018: Analytical Chemistry
Lisa A Holland, Jeffrey S Carver, Lindsay M Veltri, Rachel J Henderson, Kimberly D Quedado
Nanotechnology is a broad field combining traditional scientific disciplines; however, analytical chemistry plays an important role in material design, synthesis, characterization, and application. This article emphasizes the uniqueness of nanotechnology and the importance of providing high-quality undergraduate research experiences to both attract and retain talented individuals to the field of nanotechnology. In response to this need to develop a strong and sustainable nanotechnology work force, strategies to create authentic research experiences are considered within the framework of an interdisciplinary nanotechnology environment at West Virginia University...
September 2018: Analytical and Bioanalytical Chemistry
Takashi Kaneta
Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules...
August 6, 2018: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
Aafrin M Pettiwala, Prabhat K Singh
Constructing sensor systems for rapid and selective detection of small biomolecules such as amino acids is a major area of focus in bioanalytical chemistry. Considering the biological relevance of arginine and lysine, significant efforts have been directed to develop fluorescent sensors for their detection. However, these developed sensors suffer from certain disadvantages such as poor aqueous solubility, technically demanding and time-consuming synthetic protocols, and more importantly, most of them operate through single wavelength measurements, making their performance prone to small variations in experimental conditions...
December 31, 2017: ACS Omega
Lei Chen, Biwu Liu, Zhangrun Xu, Juewen Liu
Selective extraction of a small amount of nucleic acids from complex biological samples containing a high concentration of proteins is critical for bioanalytical chemistry. A number of previously published studies have focused on long, double-stranded DNA such as plasmid DNA. On the other hand, we are interested in short oligonucleotides. Nucleic acids have a negatively charged phosphate backbone that interacts with metal oxides strongly, and this may be used to distinguish them from proteins. In this work, a few metal oxide nanoparticles were screened, including NiO, CoO, ZnO, TiO2 , CeO2 , and Fe3 O4 for DNA recovery...
August 7, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
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