keyword
https://read.qxmd.com/read/38547431/double-negative-t-cells-utilize-a-tnf%C3%AE-jak1-icam-1-cytotoxic-axis-against-acute-myeloid-leukemia
#1
JOURNAL ARTICLE
Enoch Tin, JongBok Lee, Ismat Khatri, Yoosu Na, Mark D Minden, Li Zhang
Allogeneic double-negative T cells (DNTs) are a rare T cell subset that effectively target acute myeloid leukemia (AML) without inducing graft-vs-host disease in an allogeneic setting. A phase I clinical trial demonstrated the feasibility, safety, and potential efficacy of allogeneic DNT therapy among patients with relapsed AML. However, the molecular mechanisms of DNT-mediated cytotoxicity against AML remain elusive. Thus, we utilized a flow cytometry-based high throughput screening to compare the surface molecule expression profile on DNTs during their interaction with DNT-susceptible or -resistant AML cells and identified a TNFα-dependent cytotoxic pathway in DNT-AML interaction...
March 28, 2024: Blood Advances
https://read.qxmd.com/read/38547428/eosinophil-expression-of-triggering-receptor-expressed-on-myeloid-cells-1-trem-1-restricts-type-2-lung-inflammation
#2
JOURNAL ARTICLE
Jayden L Bowen, Kathy Keck, Sankar Baruah, Kathy H Nguyen, Andrew L Thurman, Alejandro A Pezzulo, Julia Klesney-Tait
Asthma affects 25 million Americans and recent advances in treatment are effective for only a portion of severe asthma patients. Triggering Receptor Expressed on Myeloid cells 1 (TREM-1), an innate receptor that canonically amplifies inflammatory signaling in neutrophils and monocytes, plays a central role in regulating lung inflammation. It is unknown how TREM-1 contributes to allergic asthma pathology. Utilizing a murine model of asthma, flow cytometry revealed TREM-1+ eosinophils in the lung tissue and airway during allergic airway inflammation...
March 28, 2024: Journal of Leukocyte Biology
https://read.qxmd.com/read/38547355/k33-only-mutant-ubiquitin-augments-bone-marrow-derived-dendritic-cell-mediated-ctl-priming-via-pi3k-akt-pathway
#3
JOURNAL ARTICLE
Yi Yun Liang, Xiao Yan Liao, Jun Jun Jia, Yi Zhen Yin, Yue Hua Zhang, Feng Guang Gao
To explore the effect of K33 only mutant ubiquitin (K33O) on bone marrow-derived dendritic cells' (BMDCs') maturity, antigen uptake capability, surface molecule expressions and BMDC-mediated CTL priming, and further investigate the role of PI3K-Akt engaged in K33O-increased BMDC maturation, antigen uptake and presentation, surface molecule expressions and BMDC-based CTL priming. BMDCs were conferred K33O and other ubiquitin mutants (K33R, K48R, K63R-mutant ubiquitin) incubation or LY294002 and wortmannin pretreatment...
March 28, 2024: Immunology
https://read.qxmd.com/read/38546981/the-role-and-mechanism-of-nicotinamide-riboside-in-oxidative-damage-and-a-fibrosis-model-of-trabecular-meshwork-cells
#4
JOURNAL ARTICLE
Yuping Zeng, Yijun Lin, Juhua Yang, Xiaohui Wang, Yihua Zhu, Biting Zhou
PURPOSE: To investigate the potential effects and mechanism of nicotinamide riboside (NR) on the oxidative stress and fibrosis model of human trabecular meshwork (HTM) cell line cells. METHODS: HTM cells were pretreated with NR, followed by the induction of oxidative injury and fibrosis by hydrogen peroxide (H2O2) and TGF-β2, respectively. Cell viability was tested using Hoechst staining and MTT assays, cell proliferation was assessed by EdU assay, and cell apoptosis was detected by flow cytometry and western blotting...
March 1, 2024: Translational Vision Science & Technology
https://read.qxmd.com/read/38546908/resveratrol-ameliorates-glioblastoma-inflammatory-response-by-reducing-nlrp3-inflammasome-activation-through-inhibition-of-the-jak2-stat3-pathway
#5
JOURNAL ARTICLE
Chao Zhang, Qian Peng, Yuhang Tang, Chengcheng Wang, Shuai Wang, Dong Yu, Shiqiang Hou, Yu Wang, Lanlan Zhang, Ning Lin
OBJECTIVES: The aim of this study was to investigate the anti-tumor effect of resveratrol (RSV) on glioblastoma (GBM) and its specific mechanism in improving the inflammatory response of the tumor microenvironment. The tumor microenvironment of GBM is highly neuroinflammatory, inducing tumor immunosuppression. Therefore, ameliorating the inflammatory response is an important focus for anti-tumor research. METHODS: The anti-tumor effect of RSV on GBM was demonstrated through in vitro cellular assays, including CCK-8, EdU, PI staining, Transwell, wound healing assay, and flow cytometry...
March 28, 2024: Journal of Cancer Research and Clinical Oncology
https://read.qxmd.com/read/38546882/urocortin-1-promotes-colorectal-cancer-cell-migration-and-proliferation-and-inhibits-apoptosis-via-inhibition-of-the-p53-signaling-pathway
#6
JOURNAL ARTICLE
Xiaolan Guo, Ya Li, Xiangyu Chen, Binghua Sun, Xiaolan Guo
PURPOSE: To investigate the effect of urocortin-1 (UCN-1) on growth, migration, and apoptosis in colorectal cancer (CRC) in vivo and vitro and the mechanism by which UCN-1 modulates CRC cells in vitro. METHODS: The correlation between UCN-1 and CRC was evaluated using The Cancer Genome Atlas (TCGA) database and a tissue microarray. The expression of UCN-1 in CRC cells was assessed using quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting...
March 28, 2024: Journal of Cancer Research and Clinical Oncology
https://read.qxmd.com/read/38546397/piga-mutations-and-glycosylphosphatidylinositol-anchor-dysregulation-in-polyposis-associated-duodenal-tumorigenesis
#7
JOURNAL ARTICLE
Elena Meuser, Kyle Chang, Angharad Walters, Joanna J Hurley, Hannah D West, Iain Perry, Matthew Mort, Laura Reyes-Uribe, Rebekah Truscott, Nicholas Jones, Rachel Lawrence, Gareth Jenkins, Peter Giles, Sunil Dolwani, Bilal Al-Sarireh, Neil Hawkes, Emma Short, Geraint T Williams, Melissa W Taggart, Kim Luetchford, Patrick M Lynch, Diantha Terlouw, Maartje Nielsen, Sarah-Jane Walton, Andrew Latchford, Susan K Clark, Julian R Sampson, Eduardo Vilar, Laura E Thomas
The pathogenesis of duodenal tumours in the inherited tumour syndromes Familial Adenomatous Polyposis (FAP) and MUTYH-associated Polyposis (MAP) is poorly understood. This study aimed to identify genes that are significantly mutated in these tumours and to explore the effects of these mutations. Whole exome and whole transcriptome sequencing identified recurrent somatic coding variants of PIGA in 19/70 (27%) FAP and MAP duodenal adenomas, and further confirmed the established driver roles for APC and KRAS. PIGA catalyses the first step in glycosylphosphatidylinositol (GPI) anchor biosynthesis...
March 28, 2024: Molecular Cancer Research: MCR
https://read.qxmd.com/read/38546290/circ_0027791-contributes-to-the-growth-and-immune-evasion-of-hepatocellular-carcinoma-via-the-mir-496-programmed-cell-death-ligand-1-axis-in-an-m6a-dependent-manner
#8
JOURNAL ARTICLE
Furong Yu, Peifei Fang, Yonghong Fang, Daojun Chen
Emerging evidence indicates the critical roles of circular RNAs in the development of multiple cancers, containing hepatocellular carcinoma (HCC). Herein, our present research reported the biological function and mechanism of circ_0027791 in HCC progression. Circ_0027791, microRNA-496 (miR-496), programmed cell death ligand 1 (PDL1), and methyltransferase-like 3 (METTL3) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Cell viability, proliferation, invasion, and sphere formation ability were detected using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, 5-ethynyl-2'-deoxyuridine, transwell, and sphere formation assays...
March 28, 2024: Environmental Toxicology
https://read.qxmd.com/read/38545824/effect-of-galectin-1-on-prognosis-and-responsiveness-of-immune-checkpoint-plus-tyrosine-kinase-inhibition-in-renal-cell-carcinoma
#9
JOURNAL ARTICLE
Jiajun Wang, Sihong Zhang, Ying Wang, Yanjun Zhu, Xianglai Xu, Jianming Guo
BACKGROUND: In renal cell carcinoma (RCC), no clinically available biomarker has been utilized for checkpoint inhibitor immunotherapy (IO) + tyrosine kinase inhibitor (TKI) combinations. Galectin-1 overexpression is found in tumors, with potential immune-regulating roles. METHODS: RNA-sequencing was performed in two cohorts of RCC treated with IO/TKI combination therapy (ZS-MRCC, JAVELIN-101). Immunohistochemistry and flow cytometry were performed to investigate immune cell infiltration and function in the tumor microenvironment of RCC...
April 2024: Cancer Medicine
https://read.qxmd.com/read/38545766/the-value-of-nerve-ultrasound-to-diagnose-and-follow-up-the-multifocal-neurolyphomatosis-in-the-upper-limb-case-report-and-literature-review
#10
Nan Zhuang, Lu Xie, Dongsheng Liu, HaiQin Xie
INTRODUCTION: Neurolymphomatosis (NL) is a rare disease. Ultrasound (US) plays a crucial role in diagnosing and following up the NL. CASE PRESENTATION: A 59-year-old man was hospitalized with acute pain in the left upper extremity. Ultrasound revealed segmental swelling of multiple nerves around his left elbow with abundant blood flow signals. Contrast-Enhanced Ultrasound (CEUS) showed a rapid, complete and homogenous enhancement in the nerve lesions in the early arterial phase...
2024: Current medical imaging
https://read.qxmd.com/read/38545187/role-of-bushen-huoxue-formula-and-transplanted-endothelial-progenitor-cells-play-in-promoting-endplate-microcirculation-and-attenuating-intervertebral-disc-degeneration
#11
JOURNAL ARTICLE
Yue Xie, Jianpo Zhang, Shengqi Yang, Weifeng Zhai, Hailiang Zhao, Zhan Shen, Ji Guo, Yongwei Jia
OBJECTIVE: To explore whether Bushen Huoxue Formula (BSHXF) improves the angiogenesis ability of transplanted endothelial progenitor cells (EPCs) in endplate and its potential mechanism in delaying intervertebral disc degeneration (IDD). METHODS: BSHXF was analyzed via Ultra-High Performance Liquid Chromatography (UPLC). Rabbit axial compression lumbar IDD models were constructed and the effects of BSHXF, EPCs, and their combination in IDD were determined by MRI, histological evaluation, TUNEL, and immunofluorescence assays...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38545136/characteristics-of-lymphocyte-subsets-and-inflammatory-factors-in-patients-with-covid-19
#12
JOURNAL ARTICLE
Zixi Chen, Jinpeng Li, Jin Zheng, Fenfen Xiang, Xiaoxiao Li, Mengzhe Zhang, Xiangdong Kang, Rong Wu
OBJECTIVE: This research aims to examine the involvement of lymphocyte subsets and inflammatory cytokines in the development and progression of COVID-19. METHODS: 164 COVID-19 patients were admitted to hospital between December 2022 and January 2023. Based on lung CT scans and whether it is necessary for intensive care unit (ICU) admission, they were categorized into: severe groups (84) and mild disease groups (80). Peripheral blood were also collected from 101 healthy examinees and 164 patients...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38545102/immune-cell-expression-patterns-of-cd39-cd73-ectonucleotidases-in-rodent-models-of-cardiac-arrest-and-resuscitation
#13
JOURNAL ARTICLE
Tomoaki Aoki, Vanessa Wong, Tai Yin, Eriko Nakamura, Yusuke Endo, Kei Hayashida, Simon C Robson, Harshal Nandurkar, Betty Diamond, Sun Jung Kim, Atsushi Murao, Ping Wang, Lance B Becker, Koichiro Shinozaki
BACKGROUND: Cardiac arrest (CA) is a significant public health concern. There is the high imminent mortality and survival in those who are resuscitated is substantively compromised by the post-CA syndrome (PCAS), characterized by multiorgan ischemia-reperfusion injury (IRI). The inflammatory response in PCAS is complex and involves various immune cell types, including lymphocytes and myeloid cells that have been shown to exacerbate organ IRI, such as myocardial infarction. Purinergic signaling, as regulated by CD39 and CD73, has emerged as centrally important in the context of organ-specific IRI...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38545097/tigit-regulates-cd4-t-cell-immunity-against-polymicrobial-sepsis
#14
JOURNAL ARTICLE
Xuexin Zhong, Haiping Xie, Shuang Wang, Tingting Ren, Junlin Chen, Yuefang Huang, Niansheng Yang
BACKGROUND: Sepsis is one of the major causes of death and increased health care burden in modern intensive care units. Immune checkpoints have been prompted to be key modulators of T cell activation, T cell tolerance and T cell exhaustion. This study was designed to investigate the role of the negative immune checkpoint, T cell immunoglobulin and ITIM domain (TIGIT), in the early stage of sepsis. METHOD: An experimental murine model of sepsis was developed by cecal ligation and puncture (CLP)...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38544929/silencing-klf6-alleviates-cigarette-smoke-extract-induced-mitochondrial-dysfunction-in-bronchial-epithelial-cells-by-sirt4-upregulation
#15
JOURNAL ARTICLE
Menghong Wan, Chen Wang, Jiamin Cui, Qing Xia, Lei Zhang
BACKGROUND: The incidence of chronic obstructive pulmonary disease (COPD) is increasing year by year. Kruppel-like factor 6 (KLF6) plays an important role in inflammatory diseases. However, the regulatory role of KLF6 in COPD has not been reported so far. METHODS: The viability of human bronchial epithelial cells BEAS-2B induced by cigarette smoke extract (CSE) was detected by CCK-8 assay. The protein expression of KLF6 and sirtuin 4 (SIRT4) was appraised with Western blot...
2024: International Journal of Chronic Obstructive Pulmonary Disease
https://read.qxmd.com/read/38544388/resveratrol-and-tetrahydroisoquinoline-effects-on-neutrophil-sensitivity-to-netosis-formation-in-low-risk-essential-thrombocythemia-patients
#16
JOURNAL ARTICLE
Fahimeh Shahriyary, Mohammad Reza Amirzargar, Mahshid Vafajoo, Ahmad Kooshari, Ali Basi, Seyed Mohsen Razavi, Behnaz Gharegozlou, Minoo Shahidi
INTRODUCTION: Recent studies scrutinize how NETosis (a unique cell death mechanism of neutrophil), impacts thrombosis patients with essential thrombocythemia (ET). This research evaluates the susceptibility of ET neutrophils to form NETs and tests two potential inhibitors, resveratrol (RSV) and tetrahydroisoquinoline (THIQ), in vitro. METHODS: Platelet-rich plasma from low-risk ET patients was used, along with neutrophils from both patients and controls. NET formation assays, with or without RSV and THIQ treatment after LPS stimulation, were conducted in a CO2 incubator...
March 27, 2024: European Journal of Haematology
https://read.qxmd.com/read/38543924/mdck-adaptive-mutation-of-a169s-changes-glycosylation-pattern-of-hemagglutinin-and-enhances-mdck-based-h7n9-vaccine-virus-production-without-loss-of-antigenicity-and-immunogenicity
#17
JOURNAL ARTICLE
Po-Ling Chen, Tsai-Chuan Weng, Chia-Chun Lai, Tsai-Teng Tzeng, Min-Han Lin, Kai-Chieh Hu, Alan Yung-Chih Hu, Min-Shi Lee, Wang-Chou Sung
The adaptation of egg-derived H7N9 candidate vaccine virus (CVV) in the mammalian cell line is an approach to developing a high-growth virus strain for the mass production of vaccine manufacturing. The adaptive mutations that occur in hemagglutinin (HA) are critical to the activity and potency of the vaccine virus. Previously, we identified a new mutation of A169S in the HA protein of an MDCK-adapted H7N9 vaccine virus (A/Anhui/2013, RG268); however, whether and how this mutation affects vaccine potency remain to be investigated...
March 11, 2024: Vaccines
https://read.qxmd.com/read/38543915/flublok-quadrivalent-vaccine-adjuvanted-with-r-dotap-elicits-a-robust-and-multifunctional-cd4-t-cell-response-that-is-of-greater-magnitude-and-functional-diversity-than-conventional-adjuvant-systems
#18
JOURNAL ARTICLE
Chantelle L White, Maryah A Glover, Siva K Gandhapudi, Katherine A Richards, Andrea J Sant
It is clear that new approaches are needed to promote broadly protective immunity to viral pathogens, particularly those that are prone to mutation and escape from antibody-mediated immunity. CD4+ T cells, known to target many viral proteins and highly conserved peptide epitopes, can contribute greatly to protective immunity through multiple mechanisms. Despite this potential, CD4+ T cells are often poorly recruited by current vaccine strategies. Here, we have analyzed a promising new adjuvant (R-DOTAP), as well as conventional adjuvant systems AddaVax with or without an added TLR9 agonist CpG, to promote CD4+ T cell responses to the licensed vaccine Flublok containing H1, H3, and HA-B proteins...
March 7, 2024: Vaccines
https://read.qxmd.com/read/38543900/toward-a-sars-cov-2-vlp-vaccine-hbc-g-as-a-carrier-for-sars-cov-2-spike-rbm-and-nucleocapsid-protein-derived-peptides
#19
JOURNAL ARTICLE
Ivars Petrovskis, Dace Skrastina, Juris Jansons, Andris Dislers, Janis Bogans, Karina Spunde, Anastasija Neprjakhina, Jelena Zakova, Anna Zajakina, Irina Sominskaya
Virus-like particles (VLPs) offer an attractive possibility for the development of vaccines. Recombinant core antigen (HBc) of Hepatitis B virus (HBV) was expressed in different systems, and the E. coli expression system was shown to be effective for the production of HBc VLPs. Here, we used HBc of the HBV genotype G (HBc/G) as a technologically promising VLP carrier for the presentation of spike RBM and nucleocapsid protein-derived peptides of the SARS-CoV-2 Delta variant for subsequent immunological evaluations of obtained fusion proteins...
March 4, 2024: Vaccines
https://read.qxmd.com/read/38543858/the-long-term-immunogenicity-of-mrnabnt162b-third-vaccine-dose-in-solid-organ-transplant-recipients
#20
JOURNAL ARTICLE
Maria Antonella Zingaropoli, Mariasilvia Guardiani, Federica Dominelli, Eeva Tortellini, Manuela Garofalo, Francesco Cogliati Dezza, Anastasia Centofanti, Carolina Carillo, Anna Napoli, Federico Venuta, Claudio Maria Mastroianni, Renzo Pretagostini, Miriam Lichtner, Maria Rosa Ciardi, Gianluca Russo
We investigated humoral and T-cell response to a SARS-CoV-2 mRNA vaccine in solid organ transplant recipients (SOT-Rs) and healthy donors (HDs) before (T0) and after two (T1) and twelve months (T2) since the third dose administration. SOT-Rs were stratified according to the transplanted organ and to the time elapsed since the transplant. In SOT-Rs, detectable levels of anti-S antibodies were observed in 44%, 81% and 88% at T0, T1 and T2, respectively. Conversely, anti-S antibody levels were detected in 100% of HD at all time points...
February 22, 2024: Vaccines
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