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Protein Corona

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https://read.qxmd.com/read/30768246/exploiting-protein-corona-around-gold-nanoparticles-conjugated-to-p53-activating-peptide-to-increase-the-level-of-stable-p53-proteins-in-cells
#1
Kian Ping Chan, Sheng-Hao Chao, James Chen Yong Kah
Therapeutic peptides suffer from major drawbacks such as peptide degradation in vivo due to proteolysis. Gold nanoparticles (AuNPs) are an effective carrier for therapeutic peptides that improve their stability in vivo, while also enabling non-specific adsorption of complementary proteins to enhance their effectiveness. Using p53 peptide as a model known to disrupt the intracellular MDM2-p53 protein-protein interaction which tags the endogenous p53 proteins for degradation, we conjugated p53 peptides to AuNPs (AuNP-p53) and examined the functionality of AuNP-p53 to release the endogenous p53 proteins from being tagged for degradation, thereby increasing the level of stable p53 proteins in acute myeloid leukemia 2 (AML2) cells...
February 15, 2019: Bioconjugate Chemistry
https://read.qxmd.com/read/30766868/sers-probing-of-proteins-in-gold-nanoparticle-agglomerates
#2
Gergo Peter Szekeres, Janina Kneipp
The collection of surface-enhanced Raman scattering (SERS) spectra of proteins and other biomolecules in complex biological samples such as animal cells has been achieved with gold nanoparticles that are introduced to the sample. As a model for such a situation, SERS spectra were measured in protein solutions using gold nanoparticles in the absence of aggregating agents, allowing for the free formation of a protein corona. The SERS spectra indicate a varied interaction of the protein molecule with the gold nanoparticles, depending on protein concentration...
2019: Frontiers in Chemistry
https://read.qxmd.com/read/30758528/liposome-protein-corona-characterization-as-a-new-approach-in-nanomedicine
#3
REVIEW
Anna Laura Capriotti, Chiara Cavaliere, Susy Piovesana
This trends article describes the analytical approaches for the in-depth characterization of the protein corona on liposome nanoparticles. In particular, examples since 2014 are summarized according to the analytical approach. Traditional protein corona characterizations from in vitro static experiments are provided along with the newly introduced experimental setups for characterization of the protein corona by in vitro dynamic and in vivo studies. Additionally, a special attention is also devoted to the need for introduction of new experimental workflows for characterization of a much wider array of biomolecules...
February 13, 2019: Analytical and Bioanalytical Chemistry
https://read.qxmd.com/read/30758189/protein-engineered-nanoscale-micelles-for-dynamic-19-f-magnetic-resonance-and-therapeutic-drug-delivery
#4
Lindsay K Hill, Joseph A Frezzo, Priya Katyal, Minh Dung Hoang, Zakia Ben Youss, Cynthia Xu, Xuan Xie, Erika Delgado-Fukushima, Youssef Zaim Wadhghiri, Jin Kim Montclare
Engineered proteins provide an interesting template for designing fluorine-19 (19 F) magnetic resonance imaging (MRI) contrast agents, yet progress has been hindered by the unpredictable relaxation properties of fluorine. Herein, we present the biosynthesis of a protein block copolymer, termed "<u>f</u>luorinated <u>t</u>hermo-<u>r</u>esponsive <u>a</u>ssembled <u>p</u>rotein" (F-TRAP), which assembles into a monodisperse nanoscale micelle with interesting 19 F nuclear magnetic resonance relaxation (NMR) properties and the ability to encapsulate and release small therapeutic molecules, imparting potential as a diagnostic and therapeutic (theranostic) agent...
February 13, 2019: ACS Nano
https://read.qxmd.com/read/30741295/a-protein-corona-study-by-scattering-correlation-spectroscopy-a-comparative-study-between-spherical-and-urchin-shaped-gold-nanoparticles
#5
Hanane Moustaoui, Justine Saber, Ines Djeddi, Qiqian Liu, Dania Movia, Adriele Prina-Mello, Jolanda Spadavecchia, Marc Lamy de la Chapelle, Nadia Djaker
The study of protein interactions with gold nanoparticles (GNP) is a key step prior to any biomedical application. These interactions depend on many GNP parameters such as size, surface charge, chemistry, and shape. In this work, we propose to use a sensitive technique named scattering correlation spectroscopy or SCS to study protein interactions with GNP. SCS allowed the investigation of the GNP hydrodynamic radius with a very high sensitivity before and after interaction with proteins. No labeling is needed...
February 11, 2019: Nanoscale
https://read.qxmd.com/read/30733474/molecular-interaction-of-fibrinogen-with-zeolite-nanoparticles
#6
Hossein Derakhshankhah, Atiyeh Hosseini, Fereshteh Taghavi, Samira Jafari, Alireza Lotfabadi, Mohammad Reza Ejtehadi, Sahba Shahbazi, Ali Fattahi, Atiyeh Ghasemi, Ebrahim Barzegari, Mina Evini, Ali Akbar Saboury, Seyed Mehdi Kamali Shahri, Behnaz Ghaemi, Eng-Poh Ng, Hussein Awala, Fatemeh Omrani, Iraj Nabipour, Mohammad Raoufi, Rassoul Dinarvand, Koorosh Shahpasand, Svetlana Mintova, Mohammad Javad Hajipour, Morteza Mahmoudi
Fibrinogen is one of the key proteins that participate in the protein corona composition of many types of nanoparticles (NPs), and its conformational changes are crucial for activation of immune systems. Recently, we demonstrated that the fibrinogen highly contributed in the protein corona composition at the surface of zeolite nanoparticles. Therefore, understanding the interaction of fibrinogen with zeolite nanoparticles in more details could shed light of their safe applications in medicine. Thus, we probed the molecular interactions between fibrinogen and zeolite nanoparticles using both experimental and simulation approaches...
February 7, 2019: Scientific Reports
https://read.qxmd.com/read/30721790/proteomic-analysis-of-the-bio-corona-formed-on-the-surface-of-au-ag-pt-nanoparticles-in-human-serum
#7
María Del Pilar Chantada-Vázquez, Antonio Castro López, Susana B Bravo, Sergio Vázquez-Estévez, Benigno Acea-Nebril, Cristina Núñez
Adsorption of biomolecules onto nanoparticles surface in biological samples led to the formation of a bio-corona, it could modified the "identity" of nanoparticles, contributing to the determination of their toxicity and biocompatibility. Gel electrophoresis in combination with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to qualitatively analyze and identify the human serum proteins adsorbed on the surface of three different nanomaterials stabilized with citrate: 10.02 ± 0...
January 29, 2019: Colloids and Surfaces. B, Biointerfaces
https://read.qxmd.com/read/30721043/formation-of-protein-corona-on-nanoparticles-with-digestive-enzymes-in-simulated-gastrointestinal-fluids
#8
Yihui Wang, Man Li, Xingfeng Xu, Wenting Tang, Liu Xiong, Qingjie Sun
The protein corona (PC) that defines the biological identity of nanoparticles in the blood is well known, but no comprehensive and systematic study has been conducted yet on the formation of PC in the gastrointestinal environment. Thus, this study aimed to explore the interaction between model polystyrene nanoparticles (PS-NPs) with 50-100 nm and three digestive enzymes, namely, pepsin, α-amylase, and trypsin. Results showed that the thickness of the PC formed by α-amylase and trypsin was 25-100 and 50-100 nm, respectively...
February 5, 2019: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/30701500/expression-pattern-of-plant-mirnas-by-classical-transcriptional-fusion-constructs
#9
Andrea Tovar-Aguilar, Karla A Sánchez-Elizondo, Alejandra Rodríguez-Rodríguez, Marcos I González-Jaime, Genaro Patiño-López, Vadim Perez-Koldenkova, Jesús A Badillo-Corona, Noé V Durán-Figueroa
microRNAs are noncoding RNAs of 20-24 nucleotides (nt) in length that act as repressors of genes and are important in key developmental processes in the entire life cycle of plants. To determine the function of a microRNA, the first step is to resolve its expression pattern; this can be achieved by in situ hybridization, RNA blot assays, or quantitative PCR. However, the study of the expression of a MIR gene is straightforward with the use of reporter proteins such as β-D-glucuronidase (GUS), GFP, or mCherry...
2019: Methods in Molecular Biology
https://read.qxmd.com/read/30698447/role-of-ligand-conformation-on-nanoparticle-protein-interactions
#10
Federica Simonelli, Giulia Rossi, Luca Monticelli
Engineered biomedical nanoparticles (NP) administered via intravenous routes are prone to associate to serum proteins. The protein corona can mask the NP surface functionalization and hamper the delivery of the NP to its biological target. The design of corona-free NPs relies on our understanding of the chemical-physical features of the NP surface driving the interaction with serum proteins. Here we address, by computational means, the interaction between human serum albumin (HSA) and a prototypical monolayer-protected Au nanoparticle...
January 30, 2019: Journal of Physical Chemistry. B
https://read.qxmd.com/read/30697892/artificial-cellulosome-complex-from-the-self-assembly-of-ntani-functionalized-polymeric-micelles-and-cellulases
#11
Qian Wang, Lin Lu, Libo Zhang, Liang Yuan, Tianyu Zhu, Wilfred Chen, Guiren Wang
Polymer-protein core-shell nanoparticles have been explored for enzyme immobilization. This work reports the development of functional polymeric micelles for immobilizing 6×His-tagged cellulases with controlled spatial orientation of enzymes, resulting in "artificial cellulosomes" for effective cellulose hydrolysis. Poly(styrene)-b-poly(styrene-alt-maleic anhydride) (PS-b-PSMA) was prepared through one-pot reversible addition-fragmentation chain-transfer (RAFT) polymerization and modified by nitrilotriacetic acid (NTA) to afford an amphiphilic block copolymer...
January 29, 2019: Chembiochem: a European Journal of Chemical Biology
https://read.qxmd.com/read/30696847/nanoparticle-administration-method-in-cell-culture-alters-particle-cell-interaction
#12
Thomas L Moore, Dominic A Urban, Laura Rodriguez-Lorenzo, Ana Milosevic, Federica Crippa, Miguel Spuch-Calvar, Sandor Balog, Barbara Rothen-Rutishauser, Marco Lattuada, Alke Petri-Fink
As a highly interdisciplinary field, working with nanoparticles in a biomedical context requires a robust understanding of soft matter physics, colloidal behaviors, nano-characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out to understand how in vitro technique, specifically the way we administer particles in 2D culture, can influence experimental outcomes. Gold nanoparticles coated with poly(vinylpyrrolidone) were added to J774A...
January 29, 2019: Scientific Reports
https://read.qxmd.com/read/30693754/-ultra-mixing-a-simple-and-effective-method-to-obtain-controlled-and-stable-dispersions-of-graphene-oxide-in-cell-culture-media
#13
Giacomo Reina, Amalia Ruiz, Diane Murera, Yuta Nishina, Alberto Bianco
The last decade has seen an increase in the application of graphene oxide (GO) in the biomedical field. GO has been successfully exploited for its ability to deliver many kinds of drugs into target cells. However, GO toxicity assessment is still controversial. Several studies demonstrated that GO protein coating is crucial to alleviate the material's toxicity. Besides, coronation leads to the formation of big agglomerates, reducing the cellular uptake of the material and thus its therapeutic efficiency. In this work, we propose a simple and efficient method based on rapid (Ultra-Turrax, UT) mixing to control protein corona formation...
January 29, 2019: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/30662985/viral-nanoparticles-can-elude-protein-barriers-exploiting-rather-than-imitating-nature
#14
Alberto Berardi, Francesca Baldelli Bombelli, Eva C Thuenemann, George P Lomonossoff
Protein-corona formation in body fluids and/or entrapment of nanoparticles in protein matrices (e.g. food and mucus) can hinder the delivery of nanoparticles, irrespective of the route of administration. Here we demonstrate that certain viral nanoparticles (VNPs) can evade the adhesion of a broad panel of macromolecules from several biological milieus. We also show that the permeability of VNPs through mucin gels is far superior to that of synthetic nanoparticles. The non-sticky nature of VNPs implies that they will be able to readily cross most non-specific protein and glycoprotein barriers encountered, ubiquitously, upon administration through mucosal, and non-mucosal routes...
January 21, 2019: Nanoscale
https://read.qxmd.com/read/30654677/pegylated-magnetic-mesoporous-silica-nanoparticles-decorated-with-as1411-aptamer-as-a-targeting-delivery-system-for-cytotoxic-agents
#15
Ramin Sakhtianchi, Behrad Darvishi, Zahra Mirzaie, Farid Dorkoosh, Saeed Shanehsazzadeh, Rassoul Dinarvand
Fulfilling the purpose of developing a NP with theragnostic capabilities, the current study describes synthesis of an aptamer-functionalized PEG-coated SPION/mesoporous silica core-shell nanoparticle for concurrent cancer targeted therapy and magnetic resonance imaging. SPIONs were synthesized according to a thermal decomposition method and served as cores for SPION/mesoporous silica core/shell nanoparticles (MMSNs). Doxorubicin was then successfully loaded in MMSNs which were then coated with di-carboxylic acid functionalized poly ethylene glycol (PEG-MMSNs)...
January 18, 2019: Pharmaceutical Development and Technology
https://read.qxmd.com/read/30651563/the-cell-cycle-regulator-gpsb-functions-as-cytosolic-adaptor-for-multiple-cell-wall-enzymes
#16
Robert M Cleverley, Zoe J Rutter, Jeanine Rismondo, Federico Corona, Ho-Ching Tiffany Tsui, Fuad A Alatawi, Richard A Daniel, Sven Halbedel, Orietta Massidda, Malcolm E Winkler, Richard J Lewis
Bacterial growth and cell division requires precise spatiotemporal regulation of the synthesis and remodelling of the peptidoglycan layer that surrounds the cytoplasmic membrane. GpsB is a cytosolic protein that affects cell wall synthesis by binding cytoplasmic mini-domains of peptidoglycan synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for the interaction of GpsB with peptidoglycan synthases from three bacterial species (Bacillus subtilis, Listeria monocytogenes and Streptococcus pneumoniae) and suggest their importance for cell wall growth and viability in L...
January 16, 2019: Nature Communications
https://read.qxmd.com/read/30650541/protein-corona-fingerprints-of-liposomes-new-opportunities-for-targeted-drug-delivery-and-early-detection-in-pancreatic-cancer
#17
Sara Palchetti, Damiano Caputo, Luca Digiacomo, Anna Laura Capriotti, Roberto Coppola, Daniela Pozzi, Giulio Caracciolo
Pancreatic ductal adenocarcinoma (PDAC) is the fourth cause of cancer-related mortality in the Western world and is envisaged to become the second cause by 2030. Although our knowledge about the molecular biology of PDAC is continuously increasing, this progress has not been translated into better patients' outcome. Liposomes have been used to circumvent concerns associated with the low efficiency of anticancer drugs such as severe side effects and damage of healthy tissues, but they have not resulted in improved efficacy as yet...
January 15, 2019: Pharmaceutics
https://read.qxmd.com/read/30643271/immunoglobulin-deposition-on-biomolecule-corona-determines-complement-opsonization-efficiency-of-preclinical-and-clinical-nanoparticles
#18
Vivian P Vu, Geoffrey B Gifford, Fangfang Chen, Halli Benasutti, Guankui Wang, Ernest V Groman, Robert Scheinman, Laura Saba, Seyed Moein Moghimi, Dmitri Simberg
Deposition of complement factors (opsonization) on nanoparticles may promote clearance from the blood by macrophages and trigger proinflammatory responses, but the mechanisms regulating the efficiency of complement activation are poorly understood. We previously demonstrated that opsonization of superparamagnetic iron oxide (SPIO) nanoworms with the third complement protein (C3) was dependent on the biomolecule corona of the nanoparticles. Here we show that natural antibodies play a critical role in C3 opsonization of SPIO nanoworms and a range of clinically approved nanopharmaceuticals...
January 14, 2019: Nature Nanotechnology
https://read.qxmd.com/read/30629976/from-the-microscope-to-the-magnet-disconnection-in-schizophrenia-and-bipolar-disorder
#19
REVIEW
Ellen Ji, Florian Lejuste, Samuel Sarrazin, Josselin Houenou
White matter (WM) abnormalities have implicated schizophrenia (SZ) and bipolar disorder (BD) as disconnection syndromes, yet the extent to which these abnormalities are shared versus distinct remains unclear. Diffusion tensor imaging (DTI) studies yield a putative measure of WM integrity while neuropathological studies provide more specific microstructural information. We therefore systematically reviewed all neuropathological (n = 12) and DTI (n = 11) studies directly comparing patients with SZ and BD...
January 7, 2019: Neuroscience and Biobehavioral Reviews
https://read.qxmd.com/read/30628448/graphene-nanoflakes-uptake-mediated-by-scavenger-receptors
#20
Fatima Alnasser, Valentina Castagnola, Luca Boselli, Margarita Esquivel-Gaon, Esen Efeoglu, Jennifer McIntyre, Hugh J Byrne, Kenneth A Dawson
The biological interactions of graphene have been extensively investigated over the last 10 years. However, very little is known about graphene interactions with the cell surface and how the graphene internalization process is driven and mediated by specific recognition sites at the interface with the cell. In this work, we propose a methodology to investigate direct molecular correlations between the biomolecular corona of graphene and specific cell receptors, showing that key protein recognition motifs, presented on the nanomaterial surface, can engage selectively with specific cell-receptors...
January 10, 2019: Nano Letters
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