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https://read.qxmd.com/read/30784099/rapid-and-reproducible-characterization-of-sickling-during-automated-deoxygenation-in-sickle-cell-disease-patients
#1
Minke A E Rab, Brigitte A van Oirschot, Jennifer Bos, Tesy H Merkx, Annet C W van Wesel, Osheiza Abdulmalik, Martin K Safo, A Birgitta Versluijs, Maite E Houwing, Marjon H Cnossen, Jurgen Riedl, Roger E G Schutgens, Gerard Pasterkamp, Marije Bartels, Eduard J van Beers, Richard van Wijk
In sickle cell disease (SCD), sickle hemoglobin (HbS) polymerizes upon deoxygenation, resulting in sickling of red blood cells (RBCs). These sickled RBCs have strongly reduced deformability, leading to vaso-occlusive crises and chronic hemolytic anemia. To date, there are no reliable laboratory parameters or assays capable of predicting disease severity or monitoring treatment effects. We here report on the oxygenscan: a newly developed method to measure RBC deformability (expressed as Elongation Index - EI) as a function of pO2 ...
February 19, 2019: American Journal of Hematology
https://read.qxmd.com/read/30783123/shrub-establishment-favoured-and-grass-dominance-reduced-in-acid-heath-grassland-systems-cleared-of-invasive-rhododendron-ponticum
#2
Gruffydd Lloyd Jones, Max Tomlinson, Rhys Owen, John Scullion, Ana Winters, Tom Jenkins, John Ratcliffe, Dylan Gwynn-Jones
Rhododendron ponticum L. is a damaging invasive alien species in Britain, favouring the moist, temperate climate, and the acidic soils of upland areas. It outshades other species and is thought to create a soil environment of low pH that may be higher in phytotoxic phenolic compounds. We investigated native vegetation restoration and R. ponticum regeneration post-clearance using heathland sites within Snowdonia National Park, Wales; one site had existing R. ponticum stands and three were restoring post-clearance...
February 19, 2019: Scientific Reports
https://read.qxmd.com/read/30782368/online-profiling-of-living-rat-brain-extracellular-ph-using-a-ph-dependent-solid-phase-extraction-scheme-coupled-with-microdialysis-sampling-and-inductively-coupled-plasma-mass-spectrometry
#3
Cheng-Kuan Su, Chia-Che Ho
In this study, a novel analytical method was developed for online profiling of living rat brain extracellular pH. It involved microdialysis (MD) sampling, introduction of metal ions (e.g., Mn, Co, Ni, Cd) as acid/base indicators, and a pH-dependent solid phase extraction (SPE) based on polymer-metal ion interactions as a sample pretreatment scheme prior to analysis through inductively coupled plasma mass spectrometry (ICP-MS). With a 10-μL microdialysate, optimization of the system provided a working pH range from 5...
May 9, 2019: Analytica Chimica Acta
https://read.qxmd.com/read/30779780/novel-polyclonal-antibody-based-rapid-gold-sandwich-immunochromatographic-strip-for-detecting-the-major-royal-jelly-protein-1-mrjp1-in-honey
#4
Yifan Zhang, Yong Chen, Yiting Cai, Zongyan Cui, Jinjie Zhang, Xiaohou Wang, Lirong Shen
Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal...
2019: PloS One
https://read.qxmd.com/read/30779325/fluorescence-turn-on-synthetic-lipid-rafts-on-supramolecular-sheets-and-hierarchical-concanavalin-a-assembly
#5
Yongju Kim, Xin Liu, Huichang Li, Myongsoo Lee
Here we report fluorescence turn-on synthetic lipid rafts by self-assembly of a cationic distyrylanthracene derivative on a negatively-charged sheet in an aqueous solution. First, the negatively-charged 2D membrane structure is formed by lateral associations of aromatic rods with carboxylate groups. Then, the synthetic rafts are provided floating on the surface of the negatively-charged sheets through electrostatic interactions. The fluorescence of the synthetic rafts is turned on due to the aggregation of positively-charged AIE dye on the sheets, facilitating monitoring for the formation of rafts...
February 19, 2019: Chemistry, An Asian Journal
https://read.qxmd.com/read/30778777/impact-of-small-municipal-solid-waste-landfill-on-groundwater-quality
#6
Grzegorz Przydatek, Włodzimierz Kanownik
The aim of this paper is to analyse changes in the physicochemical elements in groundwater in the vicinity of a small municipal solid waste landfill site located within the territory of the European Union on the basis of 7-year hydrochemical research. Samples of groundwater and leachate near the examined landfill were collected four times a year during two periods, between 2008 and 2012 during the use of the landfill and between 2013 and 2014 at the stage of its closure. The research results were analysed on the basis of general physicochemical properties: pH; total organic carbon (TOC); electrical conductivity (EC); inorganic elements: Cr, Zn, Cd, Cu, Pb, Hg; and one organic element-polycyclic aromatic hydrocarbon (PAH)...
February 19, 2019: Environmental Monitoring and Assessment
https://read.qxmd.com/read/30777322/cardiovascular-outcomes-of-patients-with-pulmonary-hypertension-undergoing-noncardiac-surgery
#7
Nathaniel R Smilowitz, Andrew Armanious, Sripal Bangalore, Harish Ramakrishna, Jeffrey S Berger
Pulmonary hypertension (PH), defined by a mean pulmonary artery pressure of >25mm Hg at rest, is strongly associated with morbidity and mortality in the perioperative period. The prevalence and outcomes of PH among patients referred for major noncardiac surgery in the United States are unknown. Patients ≥18 years of age hospitalized for noncardiac surgery were identified from Healthcare Cost and Utilization Project's National Inpatient Sample data from 2004 to 2014. Pulmonary hypertension was defined by International Classification of Diseases, Ninth Revision diagnosis codes...
February 8, 2019: American Journal of Cardiology
https://read.qxmd.com/read/30775453/analysis-of-ph-dose-dependent-growth-of-sulfate-reducing-bacteria
#8
Ivan Kushkevych, Dani Dordević, Monika Vítězová
Lower intraluminal colonic pH is an indication for the development of inflammatory bowel disease including active ulcerative colitis. Involvement of intestinal sulfate-reducing bacteria in decreasing bowel pH by the production of H2 S and acetate as well as their sensitivity has never been reported before. The study of the relative pH and survival of Desulfovibrio piger Vib-7 by monitoring sulfate reduction parameters was the aim of this work. Monitoring was done through the measurement of bacterial growth (biomass), dissimilatory sulfate reduction parameters: sulfate consumption, lactate oxidation, hydrogen sulfide and acetate production...
2019: Open Medicine (Warsaw, Poland)
https://read.qxmd.com/read/30774937/intracellular-processing-of-silica-coated-superparamagnetic-iron-nanoparticles-in-human-mesenchymal-stem-cells
#9
Richard P Harrison, Veeren M Chauhan, David Onion, Jonathan W Aylott, Virginie Sottile
Silica-coated superparamagnetic iron nanoparticles (SiMAGs) are an exciting biomedical technology capable of targeted delivery of cell-based therapeutics and disease diagnosis. However, in order to realise their full clinical potential, their intracellular fate must be determined. The analytical techniques of super-resolution fluorescence microscopy, particle counting flow cytometry and pH-sensitive nanosensors were applied to elucidate mechanisms of intracellular SiMAG processing in human mesenchymal stem cell (hMSCs)...
January 24, 2019: RSC Advances
https://read.qxmd.com/read/30774845/the-variations-of-some-salivary-parameters-as-probable-indices-of-the-hereditary-diabetes
#10
Menicagli Roberto, Marotta Ortensio
Background: Diabetes has a genetic predisposition and is generally not diagnosed for many years because hyperglycemia develops gradually, without presenting the classic symptoms of diabetes. The aim of this study is to verify whether, in a potentially genetically predisposed population, men and women under the age of 50 years, at the time of the study, not suffering from diabetes can be detected using parameters derived from initial metabolic alteration indices of the possible evolution of pathology...
2019: International Journal of Preventive Medicine
https://read.qxmd.com/read/30773392/requirement-and-duration-of-tube-feed-supplementation-among-congenital-diaphragmatic-hernia-patients
#11
Matthew K W Wong, Beth Haliburton, Ashley Graham, Eveline Lapidus-Krol, Theo J Moraes, Margaret A Marcon, Priscilla P L Chiu
PURPOSE: Oral feeds pose a challenge for congenital diaphragmatic hernia (CDH) infants. Tube feed (TF) supplementation may be required to support the achievement of normal growth. The aim of this study was to determine the duration and factors associated with TF use in CDH infants at our institution. METHODS: A single centre retrospective chart review was performed for CDH-born infants who underwent repair between 2000 to 2013 (REB #1000053124). Patient demographics, perinatal management, and feeding status of infants with at least 1-year follow-up were reviewed...
January 31, 2019: Journal of Pediatric Surgery
https://read.qxmd.com/read/30771909/facile-rapid-one-pot-synthesis-of-multifunctional-gold-nanoclusters-for-cell-imaging-hydrogen-sulfide-detection-and-ph-sensing
#12
Pengfei Gao, Min Li, Yan Zhang, Chuan Dong, Guomei Zhang, Lihong Shi, Gao Li, Mingjian Yuan, Shaomin Shuang
In this study, we have developed a facile, rapid one-pot strategy for synthesis of orange fluorescent gold nanoclusters (MBISA-AuNCs) at room temperature by using 2-Mercapto-5-benzimidazolesulfonic acid (MBISA) as both reducing and protecting reagent. The prepared AuNCs exhibited prominent advantages including high fluorescent quantum yield, good water solubility, excellent biocompatibility and low toxicity. Based on these superior properties, the AuNCs have been multi-talented applied in cell imaging, hydrogen sulfide detection and pH sensing...
May 15, 2019: Talanta
https://read.qxmd.com/read/30771652/continuous-monitoring-for-leaching-of-calcium-sulfoaluminate-cement-pastes-incorporated-with-zncl-2-under-the-attacks-of-chloride-and-sulfate
#13
Shengwen Tang, Junhui Yuan, Rongjin Cai, Xuanchun Wei, Cheng Zhao, Xinhua Cai, Zhen He, E Chen
Ionic zinc is considered as an environmental pollutant. This work systematically investigated leaching mechanisms of calcium sulfoaluminate cement (CSA) pastes incorporated with/without ZnCl2 under the attacks of chloride and/or sulfate. The leaching behaviors of CSA pastes in the leaching solution are in-situ and continuously monitored by innovative non-contact electrical impedance measurement (NCEIM) and pH meter. The dissolution and diffusion during the leaching process are experimentally identified. Other techniques are also performed to verify the finding of NCEIM: the ion chromatograph and inductively coupled plasma optical emission spectrometer reveal the leaching or decomposition sequence of CSA pastes during the leaching process...
February 11, 2019: Chemosphere
https://read.qxmd.com/read/30771445/evaluation-of-the-sensitivity-of-r-1-%C3%AF-mri-to-ph-and-macromolecular-density
#14
Syed O Ali, Petros Fessas, Joshua D Kaggie, Fulvio Zaccagna, Gavin Houston, Scott Reid, Martin J Graves, Ferdia A Gallagher
The tumor microenvironment is characteristically acidic and this extracellular acidosis is known to play a role in carcinogenesis and metastasis and can affect tumor chemosensitivity and radiosensitivity. Intracellular pH has been used as a possible biomarker of salvageable tissue in ischemic stroke. A non-invasive MRI-based approach for the determination and imaging of cerebral pH would be a powerful tool in cancer diagnosis and monitoring, as well as stroke treatment planning. Several pH-based MRI imaging approaches have been proposed but for these to be useful, disentangling the effects of pH from other parameters which may affect the measured MRI signal is crucial to ensure accuracy and specificity...
February 13, 2019: Magnetic Resonance Imaging
https://read.qxmd.com/read/30771041/validation-of-new-japanese-classification-system-for-esophageal-achalasia
#15
Ryo Kato, Kiyokazu Nakajima, Tsuyoshi Takahashi, Koji Tanaka, Yasuhiro Miyazaki, Tomoki Makino, Yukinori Kurokawa, Makoto Yamasaki, Masaki Mori, Yuichiro Doki
BACKGROUND: The fourth edition of New Japanese classification system for esophageal achalasia was revised after a long interval of 30 years in 2012. In this new system, achalasia is morphologically classified into 3 types, based on its X-ray findings. However, the system has been limitedly used in Japan and has not been fully validated in terms of its predictive capability of postoperative outcomes. The purpose of this study was to clarify the validity of new Japanese classification system for esophageal achalasia, as an index of patient characteristics and as a predictor of operative and mid/long-term postoperative outcomes...
February 15, 2019: Esophagus: Official Journal of the Japan Esophageal Society
https://read.qxmd.com/read/30767697/laparoscopic-modified-keyhole-technique-with-coated-polyester-mesh-for-treatment-of-parastomal-hernia-measures-for-improving-the-outcome
#16
Stefano Olmi, Alberto Oldani, Matteo Uccelli, Bruno Scotto, Giovanni Cesana, Francesca Ciccarese, Roberta Villa, Riccardo Giorgi
PURPOSE: Although the modified Sugarbaker technique gives good results for the treatment of parastomal hernia (PH), there are other valid options for the treatment of this frequent complication. In our practice, the laparoscopic keyhole (KH) technique, with some specific modifications, can give similar results. MATERIALS AND METHODS: We collected data on all the patients with symptomatic PHs who underwent surgical repair between January 2002 and December 2017 in our surgical department...
February 15, 2019: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://read.qxmd.com/read/30767511/an-integrated-ph-meter-during-reaction-monitoring-with-dual-reception-1h-31p-nmr-spectroscopy
#17
Neil Cox, Rainer Kuemmerle, Pierre Millard, Edern Cahoreau, Jean Marie François, Jean-Luc Parrou, Guy Lippens
Simultaneous detection of 1H and 31P NMR signals through a dual detection scheme with two receivers allows monitoring both the signals of a molecule and the pH of the solution through the resonance of the inorganic phosphate. We evaluate here the method in terms of sensitivity and ease of implementation, and show that the additional information obtained without any loss of information or increase in measuring time can be of practical importance in a number of biochemical systems.
February 15, 2019: Analytical Chemistry
https://read.qxmd.com/read/30766429/-cassia-grandis-lf-nanodispersion-is-a-hypoglycemic-product-with-a-potent-%C3%AE-glucosidase-and-pancreatic-lipase-inhibitor-effect
#18
Ariadna Lafourcade Prada, Hady Keita, Tatiane Pereira de Souza, Emerson Silva Lima, Leonard Domingo Rosales Acho, Márcia de Jesus Amazonas da Silva, José Carlos Tavares Carvalho, Jesus Rafael Rodriguez Amado
Purpose: This study aimed to evaluate the hypoglycemic effect, antioxidant, α-glucosidase and lipase inhibitory activity, and the cytotoxicity of the Cassia grandis nanodispersion (CgND). Methods: The hypoglycemic effect was evaluated in alloxan-induced diabetic mice. The particle size, polydispersion index, ζ-potential, and conductivity, as well as the drug-loaded content, were monitored in shelf-live, along a year. The delivery profile was evaluated in simulated intestinal fluids at pH 6...
February 2019: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://read.qxmd.com/read/30765389/impact-of-concomitant-administration-of-gastric-acid-suppressive-agents-and-pazopanib-on-outcomes-in-soft-tissue-sarcoma-patients-treated-within-the-eortc-62043-62072-trials
#19
Olivier Mir, Nathan Touati, Michela Lia, Saskia Litière, Axel Le Cesne, Stefan Sleijfer, Jean-Yves Blay, Michael Leahy, Robin Young, Ron H J Mathijssen, Nielka P Van Erp, Hans Gelderblom, Winette T Van der Graaf, Alessandro Gronchi
Purpose: Pazopanib is active in soft-tissue sarcoma (STS). Because pazopanib absorption is pH-dependent, coadministration with gastric acid-suppressive (GAS) agents such as proton pump inhibitors could affect exposure of pazopanib, and thereby its therapeutic effects. Experimental Design: The EORTC 62043 and 62072 were single-arm phase II and placebo-controlled phase III studies, respectively, of pazopanib in advanced STS. We first compared the outcome of patients treated with pazopanib with or without GAS agents for ≥80% of treatment duration, and subsequently using various thresholds...
February 14, 2019: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://read.qxmd.com/read/30762609/reflux-symptoms-in-gastroparesis-correlation-with-gastroparesis-symptoms-gastric-emptying-and-esophageal-function-testing
#20
Asad Jehangir, Henry P Parkman
GOAL: The goal of this study was to determine the relationship of reflux with gastroparesis (Gp), looking both at symptoms and objective testing. BACKGROUND: Gp patients often experience gastroesophageal reflux symptoms. How the severity of reflux correlates with the severity of Gp is not known. STUDY: Patients referred to our academic center with symptoms of Gp completed the Patient Assessment of Upper Gastrointestinal Symptoms, Hospital Anxiety and Depression Scale, and Patient Health Questionnaire (PHQ)-15...
February 12, 2019: Journal of Clinical Gastroenterology
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