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oocyte specific

Lívia B Campos, Érica C G Praxedes, Márcia V A Saraiva, Pierre Comizzoli, Alexandre R Silva
The extinction rate of mammalian species has been accelerated in recent decades. It therefore is important to preserve and store genetic materials in cryobanks for research purposes and subsequent production of offspring through assisted reproductive techniques. Along with the systematic collection and storage of germplasm, research efforts focusing on in vitro culture to produce mature gametes are critical. Specifically, obtaining mature oocytes from in vitro culture of preantral follicles is a great option to enhance female fertility preservation since these early follicles represent 90%-95% of the follicular population on ovarian cortex biopsy...
March 21, 2019: Biopreservation and Biobanking
Z Li, A Y Wang, M Bowman, K Hammarberg, C Farquhar, L Johnson, N Safi, E A Sullivan
STUDY QUESTION: What is the cumulative live birth rate following a 'freeze-all' strategy compared with a 'fresh-transfer' strategy? SUMMARY ANSWER: The 'freeze-all' strategy resulted in a similar cumulative live birth rate as the 'fresh-transfer' strategy among high responders (>15 oocytes retrieved) but did not benefit normal (10-15 oocytes) and suboptimal responders (<10 oocytes). WHAT IS KNOWN ALREADY: Frozen-thawed embryo transfer is associated with a decreased risk of adverse obstetric and perinatal outcomes compared with fresh embryo transfer...
2019: Human Reproduction Open
Mahnaz Heidari, Sara Darbandi, Mahsa Darbandi, Mohammad Mehdi Akhondi, Mohammad Reza Sadeghi
OBJECTIVE: Fibronectin (FN) is a multifunctional diametric glycoprotein on the surface of the sperm that plays an important role in the sperm-oocyte interaction and fertilization process. The aim of the present study was to assess the FN levels as a sperm surface biomarker for sperm selection in assisted reproductive technology. MATERIAL AND METHODS: Polyclonal antibody against human FN was produced in rabbit. Its quality, purity, and immune reactivity were assessed by SDS-PAGE and western blot...
January 22, 2019: Turkish Journal of Urology
M Verheijen, M Lienhard, Y Schrooders, O Clayton, R Nudischer, S Boerno, B Timmermann, N Selevsek, R Schlapbach, H Gmuender, S Gotta, J Geraedts, R Herwig, J Kleinjans, F Caiment
Though clinical trials for medical applications of dimethyl sulfoxide (DMSO) reported toxicity in the 1960s, later, the FDA classified DMSO in the safest solvent category. DMSO became widely used in many biomedical fields and biological effects were overlooked. Meanwhile, biomedical science has evolved towards sensitive high-throughput techniques and new research areas, including epigenomics and microRNAs. Considering its wide use, especially for cryopreservation and in vitro assays, we evaluated biological effect of DMSO using these technological innovations...
March 15, 2019: Scientific Reports
Md Abdullah Al Baki, Yonggyun Kim
Prostaglandins (PGs) are a group of eicosanoids that are C20 oxygenated polyunsaturated fatty acids. PGs can mediate various physiological processes such as immunity, salivary secretion, excretion, and reproduction in insects. The objective of this study was to determine the effect of PG on oocyte development in Spodoptera exigua, a lepidopteran insect known to biosynthesize PGs. Polytrophic ovarioles of S. exigua females exhibited follicle development in germarium, in which oocytes were distinct from nurse cells...
March 11, 2019: Journal of Insect Physiology
Julie A MacDonald, Yasushi Takai, Osamu Ishihara, Hiroyuki Seki, Dori C Woods, Jonathan L Tilly
OBJECTIVE: To test if ovarian microenvironmental cues affect oogonial stem cell (OSC) function in a species-specific manner. DESIGN: Animal and human study. SETTING: Research laboratory. PATIENT(S)/ANIMAL(S): Human ovarian cells obtained from cryopreserved ovarian cortical tissue of reproductive-age women, and ovarian cells and tissues from female C57BL/6 mice. INTERVENTION(S): Mouse ovarian tissue, mouse OSCs (mOSCs) and human OSCs (hOSCs) were analyzed for extracellular matrix (ECM) protein expression, and OSCs isolated from adult mouse and human ovaries were cultured in the absence or presence of ECM proteins without or with an integrin signaling inhibitor...
March 11, 2019: Fertility and Sterility
R Rodríguez Díaz, R Blanes Zamora, R Vaca Sánchez, J González Pérez, J C Alberto Bethencourt
SummaryHLA-G expression has been detected in early preimplantation embryos and it has been postulated that a relationship between embryonic expression of this factor and successful pregnancy may exist. Forty-six patients were prospectively selected from our centre 'Unidad de Reproducción Humana, Hospital Universitario de Canarias' for conducting this study. In all cases, metaphase II (MII) oocytes were fertilized using intracytoplasmic sperm injection 2-4 h after retrieval. Embryos were cultured individually in 20 µl droplets of G-1 medium (VitroLife) under oil at 37°C and a 6% CO2 environment...
March 15, 2019: Zygote: the Biology of Gametes and Early Embryos
Alexandra J Harvey
Mitochondria, originally of bacterial origin, are highly dynamic organelles that have evolved a symbiotic relationship within eukaryotic cells. Mitochondria undergo dynamic, stage-specific restructuring and redistribution during oocyte maturation and preimplantation embryo development, necessary to support key developmental events. Mitochondria also fulfil a wide range of functions beyond ATP synthesis, including the production of intracellular reactive oxygen species and calcium regulation, and are active participants in the regulation of signal transduction pathways...
May 1, 2019: Reproduction: the Official Journal of the Society for the Study of Fertility
Catherine M Castillo, Gregory Horne, Cheryl T Fitzgerald, Edward D Johnstone, Daniel R Brison, Stephen A Roberts
STUDY QUESTION: Has birthweight (BW) changed over time among IVF-conceived singletons? SUMMARY ANSWER: Singleton BW has increased markedly over the past 25 years. WHAT IS KNOWN ALREADY: IVF conceived singletons have had a higher incidence of low BW compared to spontaneously conceived singletons, and this has raised concerns over long-term increased risks of cardio-metabolic disease. However, few causal links between IVF procedures and BW have been robustly established, and few studies have examined whether BW has changed over time as IVF techniques have developed...
March 14, 2019: Human Reproduction
Yetunde Ibrahim, Jim Hotaling
Sperm epigenetic programming is tailored to meet the need of this specialized cell, which include its interaction with the oocyte during fertilization and early embryo development. The unique nature of the sperm epigenome has resulted in multiple studies investigating how perturbations in epigenetics might impact male fertility and early embryo development. In addition, sperm epigenetics appear to be altered by specific environmental exposures, which could provide a link for investigating the role of these triggers in somatic health of off springs produced...
May 2018: Seminars in Reproductive Medicine
María Itatí Albamonte, Mirta Susana Albamonte, Ricardo M Bou-Khair, Luis Zuccardi, Alfredo Daniel Vitullo
BACKGROUND: Normal pubertal ovary displays all stages of follicular development and a biased BAX/BCL2 protein ratio in favor of pro-apoptotic BAX protein comparable to the adult ovary. However, adolescents suffering malignant extra-gonadal disease show a limited follicle development after cytotoxic drug treatment and a reduced capacity of in vitro follicle growth. We evaluated the expression of pro- and anti-apoptotic members of the BCL2 gene family, the FAS/FAS-L proteins from the extrinsic apoptosis pathway, the germ-cell-specific marker VASA, the pluripotency marker OCT3/4, and markers of early and late apoptosis in the ovary of pubertal patients with malignant extra-gonadal disease, which received or not pre-surgery chemotherapy, entering a cryopreservation program...
March 11, 2019: Journal of Ovarian Research
Barbara Lawrenz, Carol Coughlan, Human M Fatemi
PURPOSE OF REVIEW: The aim of this review is to summarize the different aspects of luteal phase deficiency in IVF treatment and the possibilities of individualized luteal phase support. RECENT FINDINGS: After the application of human chorionic gonadotrophin (hCG) for final oocyte maturation, the vaginal route for progesterone administration is sufficient to maintain an adequate luteal phase support. New data point toward the possibility of oral medication; however, those data have yet to be confirmed in larger studies...
February 21, 2019: Current Opinion in Obstetrics & Gynecology
Waleed F A Marei, Geert Van Raemdonck, Geert Baggerman, Peter E J Bols, Jo L M R Leroy
Maternal lipolytic metabolic disorders result in a lipotoxic microenvironment in the ovarian follicular fluid (FF) which deteriorates oocyte quality. Although cellular stress response mechanisms are well defined in somatic cells, they remain largely unexplored in oocytes, which have distinct organelle structure and nuclear transcription patterns. Here we used shotgun proteomic analyses to study cellular responses of bovine oocytes and cumulus cells (CCs) after in vitro maturation under lipotoxic conditions; in the presence of pathophysiological palmitic acid (PA) concentration as a model...
March 6, 2019: Scientific Reports
Katsueki Ogiwara, Takayuki Takahashi
Ovulation denotes the discharge of fertilizable oocytes from ovarian follicles. Follicle rupture during ovulation requires extracellular matrix (ECM) degradation at the apex of the follicle. In the teleost medaka, an excellent model for vertebrate ovulation studies, LH-inducible matrix metalloproteinase 15 (Mmp15) plays a critical role during rupture. In this study, we found that follicle ovulation was inhibited not only by roscovitine, the cyclin-dependent protein kinase (CDK) inhibitor, but also by CDK9-inhibitor II, a specific CDK9 inhibitor...
March 4, 2019: Cells
Lidia Mínguez-Alarcón, Carmen Messerlian, Andrea Bellavia, Audrey J Gaskins, Yu-Han Chiu, Jennifer B Ford, Alexandra R Azevedo, John C Petrozza, Antonia M Calafat, Russ Hauser, Paige L Williams
BACKGROUND: We have previously investigated whether urinary concentrations of bisphenol A (BPA), parabens, and phthalate metabolites were individually associated with reproductive outcomes among women undergoing in vitro fertilization (IVF) treatment. However, humans are typically exposed to many man-made chemicals simultaneously. Thus, investigating one chemical at a time may not represent the effect of mixtures. OBJECTIVE: To investigate whether urinary concentrations of BPA, parabens, and phthalate metabolite mixtures are associated with reproductive outcomes among women undergoing IVF...
February 28, 2019: Environment International
Pouria HosseinNia, Mehdi Hajian, Farnoosh Jafarpour, Seyed Morteza Hosseini, Mojtaba Tahmoorespur, Mohammad Hossein Nasr-Esfahani
Objective: Two critical points of early development are the first and second lineage segregations, which are regulated by a wide spectrum of molecular and cellular factors. Gene regulatory networks, are one of the important components which handle inner cell mass (ICM) and trophectoderm (TE) fates and the pluripotency status across different mammalian species. Considering the importance of goats in agriculture and biotechnology, this study set out to investigate the dynamics of expression of the core pluripotency markers at the mRNA and protein levels...
July 2019: Cell Journal
Takako Saito, Ikuo Wada, Naokazu Inoue
IZUMO1 is a sperm acrosomal membrane protein that is essential for mammalian fertilization through recognition of JUNO on the oocyte surface and accompanying IZUMO1-JUNO complex formation. Here, we report a new Izumo1 gene splicing variant (IZUMO1_v2) with a unique 52-amino-acid-long signal sequence transcribed from Exon 1b. Although the mRNA amount of Izumo1_v2 is 76 times lower than that of the original Izumo1 (IZUMO1_v1) in the testis, the cell-oocyte assay indicates that IZUMO1_v2-expressing COS-7 cells have the ability to attach to the oocyte equivalent of IZUMO1_v1...
February 28, 2019: Scientific Reports
Chen Liu, Qiong Li, Yubin Yang
OBJECTIVE: This study aims to investigate the role of X-linked inhibitor of apoptosis protein (XIAP) in the pathogenesis of premature ovarian failure (POF) and the effects of the Modified Bazhen Decoction (MBD) in the treatment of POF. MATERIALS AND METHODS: Twenty-four eight-week-old Sprague Dawley (SD) rats were randomly divided into four groups: control group, POF group, MBD treatment group, and Fufang Ejiao Syrup (FES) treatment group. After adaptive feeding for one week, 18 SD rats in the POF, MBD and FES groups were subcutaneously injected with D-galactose (dissolved in saline) at the back of neck for eight weeks (150 mg/kg/day) to establish the POF model...
January 2019: Annals of Clinical and Laboratory Science
Yuewen Qi, Haowen Qi, Zeyuan Liu, Peiyuan He, Bingqing Li
Colorectal cancer (CRC) is the third most prevalent cancer in the world. Although great progress has been made, the specific molecular mechanism remains unclear. This study aimed to explore the differentially expressed genes (DEGs) and underlying mechanisms of CRC using bioinformatics analysis. In this study, we identified a total of 1353 DEGs in the database of GSE113513, including 715 up- and 638 downregulated genes. Gene ontology analysis results showed that upregulated DEGs were significantly enriched in cell division, cell proliferation, and DNA replication...
February 27, 2019: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
Wenhao Zhang, Zhiyuan Chen, Qiangzong Yin, Dan Zhang, Catherine Racowsky, Yi Zhang
Genomic imprinting is an epigenetic mechanism by which genes are expressed in a parental origin-dependent manner. We recently discovered that, like DNA methylation, oocyte-inherited H3K27me3 can also serve as an imprinting mark in mouse preimplantation embryos. In this study, we found H3K27me3 is strongly biased toward the maternal allele with some associated with DNA methylation-independent paternally expressed genes (PEGs) in human morulae. The H3K27me3 domains largely overlap with DNA partially methylated domains (PMDs) and occupy developmental gene promoters...
February 26, 2019: Genes & Development
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