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Yibin Wu, Wenjie Chen, Lifeng Gong, Chongwei Ke, Huipeng Wang, Yuankun Cai
BACKGROUND G-protein receptor 125 (GPR125), as a transmembrane signal transducer, is involved in regulating cancer development. Although GPR125 is related with several cancers, its role in colorectal cancer (CRC) and the underlying mechanism are still unknown. Here, we investigated the clinical significance of GPR125 in CRC. MATERIAL AND METHODS We assessed the expression level of GPR125 in CRC tissues by analyzing 3 datasets in the Gene Expression Omnibus (GEO) database and in human samples. The correlation between GPR125 expression and clinicopathological features was further analyzed...
September 19, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Yuncheng Zhao, Shicheng Ye, Dongli Liang, Pengxiang Wang, Jing Fu, Qing Ma, Ruijiao Kong, Linghong Shi, Xueping Gong, Wei Chen, Wubin Ding, Wenjing Yang, Zijue Zhu, Huixing Chen, Xiaoxi Sun, Jun Zhu, Zheng Li, Yuan Wang
Due to differences across species, the mechanisms of cell fate decisions determined in mice cannot be readily extrapolated to humans. In this study, we developed a feeder- and xeno-free culture protocol that efficiently induced human pluripotent stem cells (iPSCs) into PLZF+/GPR125+/CD90+ spermatogonium-like cells (SLCs). These SLCs were enriched with key genes in germ cell development such as MVH, DAZL, GFRα1, NANOS3, and DMRT1. In addition, a small fraction of SLCs went through meiosis in vitro to develop into haploid cells...
February 13, 2018: Stem Cell Reports
Zheng Chen, Minghui Niu, Min Sun, Qingqing Yuan, Chencheng Yao, Jingmei Hou, Hong Wang, Liping Wen, Hongyong Fu, Fan Zhou, Zheng Li, Zuping He
Here we proposed a new concept that human spermatogonial stem cells (SSCs) can transdifferentiate into hepatocytes in vivo. We first established liver injury model of mice by carbon tetrachloride to provide proper environment for human SSC transplantation. Liver mesenchymal cells were isolated from mice and identified phenotypically. Human SSC line was recombined with liver mesenchymal cells, and they were transplanted under renal capsules of nude mice with liver injury. The grafts expressed hepatocyte hallmarks, including ALB, AAT, CK18, and CYP1A2, whereas germ cell and SSC markers VASA and GPR125 were undetected in these cells, implicating that human SSCs were converted to hepatocytes...
February 28, 2017: Oncotarget
A Maiga, S Lemieux, C Pabst, V-P Lavallée, M Bouvier, G Sauvageau, J Hébert
Acute myeloid leukemia (AML) is associated with poor clinical outcome and the development of more effective therapies is urgently needed. G protein-coupled receptors (GPCRs) represent attractive therapeutic targets, accounting for approximately 30% of all targets of marketed drugs. Using next-generation sequencing, we studied the expression of 772 GPCRs in 148 genetically diverse AML specimens, normal blood and bone marrow cell populations as well as cord blood-derived CD34-positive cells. Among these receptors, 30 are overexpressed and 19 are downregulated in AML samples compared with normal CD34-positive cells...
June 3, 2016: Blood Cancer Journal
Lindsey M Vansandt, Janelle L Livesay, Melissa Joy Dickson, Lei Li, Budhan S Pukazhenthi, Carol L Keefer
Spermatogonial stem cells (SSCs) are distinct in their ability to self-renew, transmit genetic information, and persist throughout the life of an individual. These characteristics make SSCs a useful tool for addressing diverse challenges such as efficient transgenic production in nonrodent, biomedical animal models, or preservation of the male genome for species in which survival of frozen-thawed sperm is low. A requisite first step to access this technology in felids is the establishment of molecular markers...
September 1, 2016: Theriogenology
Jingmei Hou, Minghui Niu, Linhong Liu, Zijue Zhu, Xiaobo Wang, Min Sun, Qingqing Yuan, Shi Yang, Wenxian Zeng, Yang Liu, Zheng Li, Zuping He
Spermatogonial stem cells (SSCs) have significant applications in both reproductive and regenerative medicine. However, primary human SSCs are very rare, and a human SSC line has not yet been available. In this study, we have for the first time reported a stable human SSC line by stably expressing human SV40 large T antigen. RT-PCR, immunocytochemistry, and Western blots revealed that this cell line was positive for a number of human spermatogonial and SSC hallmarks, including VASA, DAZL, MAGEA4, GFRA1, RET, UCHL1, GPR125, PLZF and THY1, suggesting that these cells are human SSCs phenotypically...
November 20, 2015: Scientific Reports
Johanna K Höglund, Bart Buitenhuis, Bernt Guldbrandtsen, Mogens S Lund, Goutam Sahana
BACKGROUND: The Nordic Red Cattle (NRC) consists of animls belonging to the Danish Red, Finnish Ayrshire, and Swedish Red breeds. Compared to the Holstein breed, NRC animals are smaller, have a shorter calving interval, lower mastitis incidence and lower rates of stillborn calves, however they produce less milk, fat and protein. Female fertility is an important trait for the dairy cattle farmer. Selection decisions in female fertilty in NRC are based on the female fertility index (FTI)...
2015: BMC Genetics
Ying Guo, Linhong Liu, Min Sun, Yanan Hai, Zheng Li, Zuping He
Spermatogonial stem cells (SSCs) can differentiate into spermatids, reflecting that they could be used in reproductive medicine for treating male infertility. SSCs are able to become embryonic stem-like cells with the potentials of differentiating into numerous cell types of the three germ layers and they can transdifferentiate to mature and functional cells of other lineages, highlighting significant applications of human SSCs for treating human diseases. However, human SSCs are very rare and a long-term culture system of human SSCs has not yet established...
August 2015: Experimental Biology and Medicine
Jun-long Wang, Shi Yang, Ru-hui Tian, Zi-jue Zhu, Ying Guo, Qing-qing Yuan, Zu-ping He, Zheng Li
OBJECTIVE: To isolate, identify and culture human spermatogonial stem cells (SSC) and then obtain purified and enriched human SSCs for research and application. METHODS: We detected the expression of CD90 in the human testis using the immunofluorescence technique and isolated human testicular spermatogenic cells by two-step enzymatic digestion, followed by differential plating and magnetic-activated cell sorting (MACS) with CD90 as an SSC marker. Then we identified the isolated CD90-positive spermatogenic cells by RT-PCR and immunocytochemistry, and meanwhile cocultured them with Sertoli cells in SG medium in vitro...
March 2015: Zhonghua Nan Ke Xue, National Journal of Andrology
Sajjad Sisakhtnezhad, Ahmad Reza Bahrami, Maryam M Matin, Hesam Dehghani, Madjid Momeni-Moghaddam, Sohrab Boozarpour, Moein Farshchian, Mahtab Dastpak
Although chicken spermatogonial stem cells (SSCs) have received considerable attention in recent years, only a few studies so far have focused on their derivation and characterization in vitro. Identification of specific molecular biomarkers and differentiation capacity of chicken SSCs would not only help us to understand cell and molecular biology of these cells, but also can contribute to their applications in biotechnology. In this regard, we found that colony-forming cells (SSCs) in newborn chicken testicular cell cultures were positive for alkaline phosphatase activity and also expressed specific markers including DAZL, STRA-8, CVH, PLZF, SPRY-1, GFRα1, GDNF, POU5F1, NANOG, GPR125, THY-1, c-KIT, and BCL6B, at mRNA level...
April 2015: In Vitro Cellular & Developmental Biology. Animal
Jörg Hamann, Gabriela Aust, Demet Araç, Felix B Engel, Caroline Formstone, Robert Fredriksson, Randy A Hall, Breanne L Harty, Christiane Kirchhoff, Barbara Knapp, Arunkumar Krishnan, Ines Liebscher, Hsi-Hsien Lin, David C Martinelli, Kelly R Monk, Miriam C Peeters, Xianhua Piao, Simone Prömel, Torsten Schöneberg, Thue W Schwartz, Kathleen Singer, Martin Stacey, Yuri A Ushkaryov, Mario Vallon, Uwe Wolfrum, Mathew W Wright, Lei Xu, Tobias Langenhan, Helgi B Schiöth
The Adhesion family forms a large branch of the pharmacologically important superfamily of G protein-coupled receptors (GPCRs). As Adhesion GPCRs increasingly receive attention from a wide spectrum of biomedical fields, the Adhesion GPCR Consortium, together with the International Union of Basic and Clinical Pharmacology Committee on Receptor Nomenclature and Drug Classification, proposes a unified nomenclature for Adhesion GPCRs. The new names have ADGR as common dominator followed by a letter and a number to denote each subfamily and subtype, respectively...
2015: Pharmacological Reviews
Y Zheng, A Thomas, C M Schmidt, C T Dann
STUDY QUESTION: Can human spermatogonia be detected in long-term primary testicular cell cultures using validated, germ cell-specific markers of spermatogonia? SUMMARY ANSWER: Germ cell-specific markers of spermatogonia/spermatogonial stem cells (SSCs) are detected in early (1-2 weeks) but not late (> 6 weeks) primary testicular cell cultures; somatic cell markers are detected in late primary testicular cell cultures. WHAT IS KNOWN ALREADY: The development of conditions for human SSC culture is critically dependent on the ability to define cell types unequivocally and to quantify spermatogonia/SSCs...
November 2014: Human Reproduction
C Sachs, B D Robinson, L Andres Martin, T Webster, M Gilbert, H-Y Lo, S Rafii, C K Ng, M Seandel
The optimal markers for human spermatogonial stem cells (SSCs) are not known. Among the genes recently linked to SSCs in mice and other animals are the basic helix-loop-helix transcription factor ID4 and the orphan G-protein-coupled receptor GPR125. While ID4 and GPR125 are considered putative markers for SSCs, they have not been evaluated for coexpression in human tissue. Furthermore, neither the size nor the character of the human spermatogonial populations that express ID4 and GPR125, respectively, are known...
July 2014: Andrology
Bita Nickkholgh, Sefika Canan Mizrak, Cindy M Korver, Saskia K M van Daalen, Andreas Meissner, Sjoerd Repping, Ans M M van Pelt
OBJECTIVE: To evaluate the degree of enrichment of spermatogonial stem cells (SSCs) from human testicular cell cultures by ITGA6+, HLA-/ITGA6+, GPR125+, and HLA-/GPR125+ magnetic-assisted cell sorting (MACS). DESIGN: Experimental basic science study. SETTING: Reproductive biology laboratory. PATIENT(S): Multiple samples of cryopreserved human testicular cells from two prostate cancer patients with normal spermatogenesis...
August 2014: Fertility and Sterility
Juho-Antti Mäkelä, Jorma Toppari, Adolfo Rivero-Müller, Sami Ventelä
Research on spermatogonia is hampered by complex architecture of the seminiferous tubule, poor viability of testicular tissue ex vivo and lack of physiologically relevant long-term culture systems. Therefore there is a need for an in vitro model that would enable long term survival and propagation of spermatogonia. We aimed at the most simplified approach to enable all different cell types within the seminiferous tubules to contribute to the creation of a niche for spermatogonia. In the present study we describe the establishment of a co-culture of mouse testicular cells that is based on proliferative and migratory activity of seminiferous tubule cells and does not involve separation, purification or differential plating of individual cell populations...
2014: PloS One
Hooman Sadri-Ardekani, Christa H Homburg, Toni M M van Capel, Henk van den Berg, Fulco van der Veen, C Ellen van der Schoot, Ans M M van Pelt, Sjoerd Repping
OBJECTIVE: To study whether acute lymphoblastic leukemia (ALL) cells survive in a human testicular cell culture system. DESIGN: Experimental laboratory study. SETTING: Reproductive biology laboratory, academic medical center. PATIENT(S): Acute lymphoblastic leukemia cells from three patients and testicular cells from three other patients. INTERVENTION(S): Acute lymphoblastic leukemia cells were cultured alone or in combination with testicular cells, at various concentrations, in a system that has recently been developed to propagate human spermatogonial stem cells...
April 2014: Fertility and Sterility
Maria Kokkinaki, Ardalan Djourabtchi, Nady Golestaneh
Recently we and two other groups have shown that human spermatogonial stem cells (SSCs) have the potential to become pluripotent in vitro in defined culture conditions and to differentiate into cells of the three embryonic germ layers. This discovery could open new avenues for autologous cell-based therapy in degenerative diseases, bypassing the ethical and immunological problems related to the human embryonic stem cells. In addition, human SSCs could be used to treat infertility in cancer survival children...
November 11, 2011: Journal of Stem Cell Research & Therapy
Xin Li, Isabelle Roszko, Diane S Sepich, Mingwei Ni, Heidi E Hamm, Florence L Marlow, Lilianna Solnica-Krezel
During vertebrate gastrulation, Wnt/planar cell polarity (PCP) signaling orchestrates polarized cell behaviors underlying convergence and extension (C&E) movements to narrow embryonic tissues mediolaterally and lengthen them anteroposteriorly. Here, we have identified Gpr125, an adhesion G protein-coupled receptor, as a novel modulator of the Wnt/PCP signaling system. Excess Gpr125 impaired C&E movements and the underlying cell and molecular polarities. Reduced Gpr125 function exacerbated the C&E and facial branchiomotor neuron (FBMN) migration defects of embryos with reduced Wnt/PCP signaling...
July 2013: Development
Leila Goharbakhsh, Arash Mohazzab, Sheida Salehkhou, Mahnaz Heidari, Amir Hassan Zarnani, Kazem Parivar, Mohammad Mehdi Akhondi
BACKGROUND: In cancer patients, chemo and radiotherapy can cause infertility by damaging spermatogenesis process. This process is based on self-renewal and differentiation of a rare population of the testicular cells called Spermatogonial Stem Cells (SSCs). Scientists have tried to isolate, enrich and culture Human spermatogonial stem cells, hoping to resolve infertility problems in cancer recovered patients in the future. METHODS: Spermatogonial stem cells were isolated and purified from human testicular biopsies sample consisting of at least 500,000 and at most 2,000,000 cells...
January 2013: Avicenna Journal of Medical Biotechnology
Jen-Fen Fu, Tzung-Hai Yen, Yu Chen, Ying-Jung Huang, Cheng-Lung Hsu, Der-Cherng Liang, Lee-Yung Shih
Oncogenic N-/KRAS mutations were frequently associated with MLL/AF10 in acute myeloid leukemia with myeloid sarcoma (MS). To study the cooperating leukemogenesis by MLL/AF10 and KRAS mutation, we retrovirally transduced MLL/AF10(OM-LZ) and KRASG12C into mouse bone marrow cells and generated two immortalized cell lines. The cells carrying cooperating MLL/AF10(OM-LZ) and KRASG12C had immature myelomonocytic phenotypes. Compared to a previously established cell line carrying MLL/AF10(OM-LZ) alone, cooperation of MLL/AF10(OM-LZ) with KRASG12C blocked the cells at a more immature myelomonocytic stage with reduced expression of monocyte/macrophage markers...
October 15, 2013: International Journal of Cancer. Journal International du Cancer
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