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Divane de Vargas, Talita Dutra Ponce, Erika Gisseth León Ramírez, Caroline Figueira Pereira, Maria do Perpétuo Socorro de Sousa Nóbrega
OBJECTIVE: To verify the association between the profile of women who seek specialized treatment for the use of psychoactive substances, their treatment adherence time and the types of substance used. METHOD: A retrospective cohort with data from medical records of women who sought care at a referral service for the use of psychoactive substances. RESULTS: The final sample consisted of 411 medical records. A significant association was observed between unemployment (p<0...
October 11, 2018: Revista da Escola de Enfermagem da U S P
Bruno Karolski, Letícia O B Cardoso, Louise H Gracioso, Cláudio A O Nascimento, Elen A Perpetuo
The methylotrophs bacteria can use methane and methanol as carbon sources to produce biopolymers including the polyhydroxybutyrate (PHB) a very promised substitute for the environment contaminant oil-derived polypropylene. This kind of bacteria can be very effective to help to decrease PHB price production and promote its use in substitution of several environment contaminant plastics. The search for methylotroph bacteria able to produce PHB is a very arduous job being necessary to grow all isolates and submit all of them to extraction processes and product characterization...
September 26, 2018: Journal of Microbiological Methods
Jane Simpson, Gerasimos Chatzidamianos, Ian Fletcher, Luis Perpetuo, Fiona J R Eccles
INTRODUCTION: Perceived control is an important concept in understanding adjustment to chronic conditions such as Parkinson's. While generic measures have been used to measure the construct in Parkinson's, no Parkinson's-specific scale currently exists. This study outlines the initial development and further validation of a free-to-use scale, the Parkinson's UK Scale of Perceived Control (PUKSoPC). METHOD: Focus groups were used to create items for the new scale...
August 15, 2018: Journal of the Neurological Sciences
Maria do Perpétuo Socorro de Sousa Nóbrega, Giovanna Bertolazzi Fernandes da Silva, Andreza Cardoso Ribeiro de Sena
OBJECTIVE: To describe the strategies of the Psychosocial Rehabilitation conducted in the Network of Psychosocial Care of the western region of the municipality of São Paulo. METHODOLOGY: Descriptive qualitative study, carried out with 123 professionals, from September 2015 to July 2016. Data processed by the software Alceste and analyzed in light of the analytical category Psychosocial Rehabilitation of Benedetto Saraceno and complementary literature. RESULTS: Three classes emerged that address the potential and scarcity of therapeutic residential services as a space to resume the daily life; importance of cultural activities for the exchange of identities and care beyond the scope of health; the potential of projects to generate work and income to regain the contractual power...
July 2, 2018: Revista Gaúcha de Enfermagem
Maria do Perpétuo Socorro de Sousa Nóbrega, Rebeca Ishii Munhoz, Joyce Rovarotto
OBJECTIVE: To construct and validate a nursing care dependency classification system for alcohol and other drugs. METHOD: A psychometric study. We used the agreement percentage and intraclass correlation coefficient for inter-rater agreement. The Receiver Operating Characteristic was used to determine values and accuracy of the cut-off points. The steps were literature review, content validation and clinical validation. RESULTS: Fifteen (15) judges, 6 nurses and 65 users of psychiatric emergency and hospitalization units participated...
2018: Revista da Escola de Enfermagem da U S P
Arlene Oliveira Souza, Maria do Perpétuo Socorro Rodrigues Chaves, Reinaldo Imbrozio Barbosa, Charles Roland Clement
INTRODUCTION: Invasive plants can impact biodiversity as well as the lives of native human populations. Natural ecosystems represent sources of natural resources essential for the subsistence and socio-cultural continuity of these social groups. Approximately 30,000 ha of Acacia mangium were planted for commercial purposes in savanna areas surrounding indigenous lands in Roraima State, Brazil, at the end of the 1990s. We examined the local ecological knowledge of indigenous Wapichana and Macuxi Amerindians, members of the Arawak and Carib linguistic families, respectively, concerning A...
May 3, 2018: Journal of Ethnobiology and Ethnomedicine
Eduardo Henrique Cunha Neves Filho, Adriana Pinheiro Bezerra Pires, Rosane Oliveira de Sant'Ana, Silvia Helena Barem Rabenhorst, Carlos Gustavo Hirth, Maria Do Perpétuo Socorro Saldanha da Cunha
Although the introduction of the perioperative chemotherapy on the management of gastric cancer has improved patients survival, heterogeneity of clinical outcomes has been evidenced in parallel to different histopathological regression pattern of gastric cancer cells. Thus, this study evaluated the tumor regression grading (TRG) in a series of post-treatment gastric tumors and its associations with HER2, MET, and FOXP3 expression. Material of 54 gastric cancer samples was available for TRG evaluation and immunohistochemistry...
May 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Carlos Gustavo Hirth, Adriele Machado Dos Santos, João Batista Gadelha de Cerqueira, Francisco Vagnaldo Fechine Jamacaru, Maria do Perpétuo Socorro Saldanha da Cunha, Conceição Aparecida Dornelas
Introduction: CD44 has been proposed as a prognostic marker and a stem cell marker but studies in patients with prostate cancer have yielded inconsistent results. Patients and Methods: Patients submitted to radical prostatectomy between 2008 and 2013 at a university hospital were followed with biannual serum PSA tests to determine the biochemical recurrence (BR). Archived paraffin blocks with neoplastic and nonneoplastic tissue were evaluated immunohistochemically for a panCD44 and MYC...
2018: BioMed Research International
Eduardo Henrique Cunha Neves Filho, Geórgia de Aguiar Feitosa Lima, Ângelo Roncalli Melo Alves, Valdenrique Macêdo de Sousa, Maria do Perpétuo Socorro Saldanha da Cunha
Mammary hibernomas are extremely rare benign tumors composed of brown fat cells, with only five cases previously reported in the literature. We report the case of a 42-year-old female patient with a painless growing mass in her right breast. A partial mastectomy was performed, and the diagnosis of hibernoma was confirmed by the histological features and the immunohistochemical profile. Although hibernoma is a benign tumor, its main differential diagnoses include aggressive lesions, making the accurate diagnosis essential to provide adequate care to the patient...
April 2018: Revista Brasileira de Ginecologia e Obstetrícia
Joana Perpetuo Assad, Ana Cristina Côrtes Gama, Juliana Nunes Santos, Max de Castro Magalhães
PURPOSE: The purpose of this study was to determine if voice amplification influenced vocal dose in female teachers with dysphonia. MATERIAL AND METHODS: This was an experimental study with comparative intrasubjects in which 15 individuals were compared in two different moments: condition 1 (C1) without voice amplification and condition 2 (C2) with voice amplification. All of them were female, kindergarten and elementary school teachers who presented organic or functional dysphonia...
November 6, 2017: Journal of Voice: Official Journal of the Voice Foundation
Sergio García Castillo, María Del Perpetuo Socorro Hoyos Vázquez, Ramón Coloma Navarro, Javier Cruz Ruiz, Francisco Javier Callejas González, Raúl Godoy Mayoral, Pedro Juan Tárraga López, José Antonio Rodríguez Montes
INTRODUCTION: Obstructive sleep apnoea (OSA) is highly prevalent in children and a major public health problem. An attempt is made to determine the clinical and polysomnographic presentation of paediatric OSA in our area. PATIENTS AND METHODS: Retrospective descriptive study of sleep tests conducted on children up to 14 years-old from 1999 to 2012 in the Sleep Unit of the University Hospital of Albacete. Age, gender, anthropometric, clinical data, indication and variables of sleep study, treatment, and outcomes were collected...
May 2018: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
Ingrid Regina Avanzi, Louise Hase Gracioso, Marcela Dos Passos Galluzzi Baltazar, Bruno Karolski, Elen Aquino Perpetuo, Claudio Augusto Oller do Nascimento
Copper mining has polluted soils and water, causing a reduction of the microbial diversity and a change in the structure of the resident bacterial communities. In this work, selective isolation combined with MALDI-TOF MS and the 16S rDNA method were used for characterizing cultivable bacterial communities from copper mining samples. The results revealed that MALDI-TOF MS analysis can be considered a reliable and fast tool for identifying copper-resistant bacteria from environmental samples at the genera level...
February 2017: Environmental Science and Pollution Research International
Christiane Maria Ayo, Fábio Batista Frederico, Rubens Camargo Siqueira, Cinara de Cássia Brandão de Mattos, Mariana Previato, Amanda Pires Barbosa, Fernando Henrique Antunes Murata, Aparecida Perpétuo Silveira-Carvalho, Luiz Carlos de Mattos
The objective of this study was to investigate the influence of the genes encoding the KIR receptors and their HLA ligands in the susceptibility of ocular toxoplasmosis. A total of 297 patients serologically-diagnosed with toxoplasmosis were selected and stratified according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of ocular manifestation observed: primary (n = 120) or recurrent (n = 28)...
November 9, 2016: Scientific Reports
Layla Gomes Siravenha, Leonardo Quintão Siravenha, Lucimar Di Paula Madeira, Aldemir B Oliveira-Filho, Luiz Fernando Almeida Machado, Rosimar Neris Martins Feitosa, Antonio Carlos Rosário Vallinoto, Marluísa de Oliveira Guimarães Ishak, Ricardo Ishak
The dental pulp is a sterile highly vascularized tissue and has been commonly used as a biological material to detect the genome of infectious agents that reach the dental tissue. Indeed, the pulp is also used to reveal past and ancient infections in the field of paleomicrobiology. The present study aimed to detect the presence of Hepatitis C virus (HCV) in a small community (approximately 400 inhabitants) in the Amazon region of Brazil (Nossa Senhora do Perpetuo Socorro, Vizeu, Para, Brazil) and standardize a technique for the detection of the virus in the dental pulp...
2016: PloS One
Fernando Henrique Antunes Murata, Marina Neves Ferreira, Natália Sahyoun Camargo, Gabriela Soria Santos, Lígia Cosentino Junqueira Franco Spegiorin, Aparecida Perpétuo Silveira-Carvalho, Vera Lucia Pereira-Chioccola, Luiz Carlos de Mattos, Cinara Cássia Brandão de Mattos
INTRODUCTION: Toxoplasmosis during pregnancy can be severe; thus, it is essential to diagnose the disease via serological tests. METHODS: An enzyme-linked immunosorbent assay (ELISA) was used to investigate anti-Toxoplasma gondii immunoglobulin A (IgA), M (IgM) and G (IgG) antibodies in 62 high-risk pregnant women. RESULTS: Forty-three (69.4%) women were positive for IgA, 31 (50%) for IgG, and 57 (91.9%) for IgM; 4 (6,5%) were positive for IgA but negative for IgM; 10 (16...
July 2016: Revista da Sociedade Brasileira de Medicina Tropical
J Costa-Rodrigues, S Carmo, I P Perpétuo, F J Monteiro, M H Fernandes
BACKGROUND: Surface topography is a key parameter in bone cells-biomaterials interactions. This study analyzed the behavior of human osteoclast precursor cells cultured over three hydroxyapatite (HA) surfaces ranging from a micro- to nanoscale topography. METHODS: HA surfaces were prepared with microsized HA particles, at 1300°C (HA1), and with nanosized HA particles at 1000°C (HA2) and 830°C (HA3). Human osteoclast precursors were cultured in the absence or presence of M-SCF and RANKL...
April 2016: Biochimica et Biophysica Acta
Inês P Perpétuo, Rita Raposeiro, Joana Caetano-Lopes, Elsa Vieira-Sousa, Raquel Campanilho-Marques, Cristina Ponte, Helena Canhão, Mari Ainola, João E Fonseca
INTRODUCTION: Ankylosing Spondylitis (AS) is characterized by excessive local bone formation and concomitant systemic bone loss. Tumor necrosis factor (TNF) plays a central role in the inflammation of axial skeleton and enthesis of AS patients. Despite reduction of inflammation and systemic bone loss, AS patients treated with TNF inhibitors (TNFi) have ongoing local bone formation. The aim of this study was to assess the effect of TNFi in the differentiation and activity of osteoclasts (OC) in AS patients...
2015: PloS One
Christiane Maria Ayo, Ana Vitória da Silveira Camargo, Fábio Batista Frederico, Rubens Camargo Siqueira, Mariana Previato, Fernando Henrique Antunes Murata, Aparecida Perpétuo Silveira-Carvalho, Amanda Pires Barbosa, Cinara de Cássia Brandão de Mattos, Luiz Carlos de Mattos
This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis...
2015: PloS One
Mariana Previato, Fábio Batista Frederico, Fernando Henrique Antunes Murata, Rubens Camargo Siqueira, Amanda Pires Barbosa, Aparecida Perpétuo Silveira-Carvalho, Cristina da Silva Meira, Vera Lúcia Pereira-Chioccola, Ricardo Gava, Plínio Pereira Martins Neto, Luiz Carlos de Mattos, Cinara Cássia Brandão de Mattos
BACKGROUND: Toxoplasmosis was recently included as a neglected disease by the Center for Disease Control. Ocular toxoplasmosis is one clinical presentation of congenital or acquired infection. The laboratory diagnosis is being used worldwide to support the clinical diagnosis and imaging. The aim of this study was to evaluate the use of serology and molecular methods to monitor acute OT in immunocompetent patients during treatment. METHODS: Five immunocompetent patients were clinically diagnosed with acute OT...
December 7, 2015: BMC Research Notes
Karina Diniz Oliveira, Emilio Carlos Elias Baracat, Rafael Lanaro, Caroline Eugeni, Ellen Ricci, Mayara Schiavon Rabello, Juliana Perpetuo de Souza, Vitoria Carneiro Gimenes, Renata Cruz Soares de Azevedo, Gustavo Pereira Fraga
OBJECTIVE: to describe the causes and severities of trauma in patients who met the criteria for alcohol abuse or dependence according to Mini International Neuropsychiatric Interview, and to display the pattern of alcohol consumption and subsequent changes one year after trauma. METHODS: a transversal and longitudinal quantitative study carried out between November 2012 and September 2013 in the ED. Medical and nursing students collected blood samples, applied the J section of the Mini International Neuropsychiatric Interview (MINI) and submitted alcohol abusers and dependents to BI...
July 2015: Revista do Colégio Brasileiro de Cirurgiões
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