Alzbeta Chabronova, Guus G H van den Akker, Bas A C Housmans, Marjolein M J Caron, Andy Cremers, Don A M Surtel, Kanin Wichapong, Mandy M J Peffers, Lodewijk W van Rhijn, Virginie Marchand, Yuri Motorin, Tim J M Welting
OBJECTIVE: Osteoarthritis-related cartilage extracellular matrix remodelling is dependent on changes in chondrocyte protein expression. Yet, the role of ribosomes in chondrocyte translation regulation is unknown. In this exploratory study, we investigated ribosomal RNA (rRNA) epitranscriptomic-based ribosome heterogeneity in human articular chondrocytes and its relevance for osteoarthritis. METHODS: Sequencing-based rRNA 2'-O-methylation profiling analysis (RiboMethSeq) was performed on non-OA primary human articular chondrocytes (n=5) exposed for 14 days to osteoarthritic synovial fluid (14 donors, pooled, 20% v/v)...
January 5, 2023: Osteoarthritis and Cartilage