Barbara Turchi, Claudia Campobasso, Arianna Nardinocchi, Jeroen Wagemans, Beatrice Torracca, Cédric Lood, Graziano Di Giuseppe, Paola Nieri, Fabrizio Bertelloni, Luca Turini, Valeria Ruffo, Rob Lavigne, Mariagrazia Di Luca
A novel temperate phage, named Hesat, was isolated by the incubation of a dairy strain of Staphylococcus aureus belonging to spa-type t127 with either bovine or ovine milk. Hesat represents a new species of temperate phage within the Phietavirus genus of the Azeredovirinae subfamily. Its genome has a length of 43,129 bp and a GC content of 35.11% and contains 75 predicted ORFs, some of which linked to virulence. This includes (i) a pathogenicity island (SaPln2), homologous to the type II toxin-antitoxin system PemK/MazF family toxin; (ii) a DUF3113 protein (gp30) that is putatively involved in the derepression of the global repressor Stl; and (iii) a cluster coding for a PVL...
April 15, 2024: Applied Microbiology and Biotechnology