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https://read.qxmd.com/read/30784929/bacterial-cellulose-membrane-associated-with-red-propolis-as-phytomodulator-improved-healing-effects-in-experimental-models-of-diabetes-mellitus
#1
Aline Picolotto, Dafiner Pergher, Guilherme Pacheco Pereira, Keilla Gomes Machado, Hernane da Silva Barud, Mariana Roesch-Ely, Mário Henrique Gonzalez, Leandro Tasso, Jozi Godoy Figueiredo, Sidnei Moura
Since early times, propolis has been used in folk medicine. The red propolis, collected in the northeast region of Brazil has been highlighted due to its popular use as an antimicrobial, with anti-inflammatory and healing properties, which are associated with its chemical composition. Here, we combine a bacterial membrane with red propolis to treat wounds of diabetic mice. This work aims to evaluate a biocurative from bacterial cellulose associated with red propolis in diabetic mice as wound healing model. Biocuratives from bacterial cellulose membrane and different extracts of red propolis were produced...
February 19, 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://read.qxmd.com/read/30772433/review-biotechnology-of-mycotoxins-detoxification-using-microorganisms-and-enzymes
#2
REVIEW
Fedia Ben Taheur, Bochra Kouidhi, Yasir Mohammed A Al Qurashi, Jalila Ben Salah-Abbès, Kamel Chaieb
Mycotoxins are secondary metabolites produced by various toxigenic fungi belonging to the genera Aspergillus, Fusarium and Penicillium. They have mutagenic, teratogenic, carcinogenic, immunosuppressive and endocrine disrupting effects on both humans and animals. The ubiquitous occurrence and heat stability of these mycotoxins during food and feed processing pose serious health problems in human and animals. Therefore successful biotechnological detoxification tools are in great demand. Recently, the use of bacteria, yeast, moulds and enzymes to mitigate/bind or biodegrade various mycotoxins is a novel strategy used for mycotoxins removal...
February 14, 2019: Toxicon: Official Journal of the International Society on Toxinology
https://read.qxmd.com/read/30771127/depression-anxiety-like-behavior-and-memory-impairment-in-mice-exposed-to-chitosan-coated-zein-nanoparticles
#3
Vinícius Silva Lima, Abraão Tiago Batista Guimarães, Amanda Pereira da Costa Araújo, Fernanda Neves Estrela, Ives Charlie da Silva, Nathalie Ferreira Silva de Melo, Leonardo Fernandes Fraceto, Guilherme Malafaia
The advent of biotechnology provided the synthesis of nanoproducts with diverse applications in the field of medicine, agriculture, food, among others. However, the toxicity of many nanoparticles (NP) currently used, which can penetrate natural systems and impact organisms, is not known. Thus, in this study, we evaluated whether the short exposure (5 days) to low concentrations of chitosan-coated zein nanoparticles (ZNP-CS) (0.2 ng/kg, 40 ng/kg, and 400.00 ng/kg) was capable of causing behavioral alterations compatible with cognitive deficit, as well as anxiety and depression-like behavior in Swiss mice...
February 15, 2019: Environmental Science and Pollution Research International
https://read.qxmd.com/read/30759423/electrobiofabrication-electrically-based-fabrication-with-biologically-derived-materials
#4
Jinyang Li, Si Wu, Eunkyoung Kim, Kun Yan, Huan Liu, Changsheng Liu, Hua Dong, Xue Qu, Xiaowen Shi, Jana Shen, William E Bentley, Gregory F Payne
While conventional materials fabrication methods focus on form and strength to achieve function, the fabrication of materials systems for emerging life science applications will need to satisfy a more subtle set of requirements. A common goal for biofabrication is to recapitulate complex biological contexts (e.g., tissue) for applications that range from animal-on-a-chip to regenerative medicine. In these cases, the materials systems will need to: (i) present appropriate surface functionalities over a hierarchy of length scales (e...
February 13, 2019: Biofabrication
https://read.qxmd.com/read/30733793/-in-silico-structural-and-functional-modelling-of-antifreeze-protein-afp-sequences-of-ocean-pout-zoarces-americanus-bloch-schneider-1801
#5
Manojit Bhattacharya, Arpita Hota, Avijit Kar, Deep Sankar Chini, Ramesh Chandra Malick, Bidhan Chandra Patra, Basanta Kumar Das
Antifreeze proteins (AFPs) are known to polypeptide components formed by certain plants, animals, fungi and bacteria which support to survive in sub-zero temperature. Current study highlighted the seven different antifreeze proteins of fish Ocean pout ( Zoarces americanus ), in which protein (amino acids sequence) were collected from National Centre for Biotechnology Information and finely characterized using several in silico tools. Such biocomputational techniques applied to figure out the physicochemical, functional and conformational characteristics of targeted AFPs...
December 2018: Journal, Genetic Engineering & Biotechnology
https://read.qxmd.com/read/30717546/-research-progress-about-molecular-identification-of-dna-barcoding-in-medicinal-animal
#6
Li Jing, Hang Su, Bo-Wen Sui, He Zhang, Dai-An Pan, Bin Qi
The use of animal medicine has a long history in China, it has the characteristics of high curative effect,strong activity, wide application and great potential. However,the circulation of animal medicine in current market mixed counterfeit variety and complex. Molecular identification technology of DNA barcoding is an emerging molecular biotechnology in recent years, it is a powerful supplement to traditional identification methods. This method can well identify animal species at the molecular level and has high accuracy, it can identify animal medicines quickly and monitor the medicine market effectively...
December 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://read.qxmd.com/read/30709688/between-mice-and-sheep-biotechnology-agricultural-science-and-animal-models-in-late-twentieth-century-edinburgh
#7
Miguel García-Sancho, Dmitriy Myelnikov
In this paper, we investigate the ways in which a group of scientists in Edinburgh worked across mice and sheep during the last quarter of the twentieth century. With this local episode, we show the utility of an interspecies perspective to investigate recent historical transformations in the life sciences. We argue that the emergence of animal biotechnology was the result of interactions between neoliberal policymakers, science administrators, molecular biologists, agricultural breeders, and the laboratory and farm organisms with which they worked...
January 29, 2019: Studies in History and Philosophy of Biological and Biomedical Sciences
https://read.qxmd.com/read/30678726/applications-of-mesenchymal-stem-cell-technology-in-bovine-species
#8
REVIEW
Amanda Baracho Trindade Hill, Fabiana Fernandes Bressan, Bruce D Murphy, Joaquim Mansano Garcia
Mesenchymal stem cells (MSCs) have received a great deal of attention over the past 20 years mainly because of the results that showed regeneration potential and plasticity that were much stronger than expected in prior decades. Recent findings in this field have contributed to progress in the establishment of cell differentiation methods, which have made stem cell therapy more clinically attractive. In addition, MSCs are easy to isolate and have anti-inflammatory and angiogenic capabilities. The use of stem cell therapy is currently supported by scientific literature in the treatment of several animal health conditions...
January 24, 2019: Stem Cell Research & Therapy
https://read.qxmd.com/read/30672944/recent-advances-in-arsenic-metabolism-in-plants-current-status-challenges-and-highlighted-biotechnological-intervention-to-reduce-grain-arsenic-in-rice
#9
Manju Shri, Pradyumna Kumar Singh, Maria Kidwai, Neelam Gautam, Sonali Dubey, Giti Verma, Debasis Chakrabarty
Arsenic (As), classified as a "metalloid" element, is well known for its carcinogenicity and other toxic effects to humans. Arsenic exposure in plants results in the alteration of the physiochemical and biological properties and consequently, loss of crop yield. Being a staple food for half of the world's population, the consumption of As-contaminated rice grain by humans may pose serious health issues and risks for food security. In this study, we have described the principal understanding of the molecular basis of arsenic toxicity and accumulation in plant parts...
January 23, 2019: Metallomics: Integrated Biometal Science
https://read.qxmd.com/read/30669572/development-of-a-bicistronic-vector-for-the-expression-of-a-crispr-cas9-mcherry-system-in-fish-cell-lines
#10
Sebastian Escobar-Aguirre, Duxan Arancibia, Amanda Escorza, Cristián Bravo, María Estela Andrés, Pedro Zamorano, Víctor Martínez
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely used in animals as an efficient genome editing tool. In fish cells, the technique has been difficult to implement due to the lack of proper vectors that use active promoters to drive the expression of both small guide RNA (sgRNA) and the S. pyogenes Cas9 (spCas9) protein within a single expression platform. Until now, fish cells have been modified using co-transfection of the mRNA of both the sgRNA and the spCas9...
January 21, 2019: Cells
https://read.qxmd.com/read/30666424/insect-microbial-symbionts-as-a-novel-source-for-biotechnology
#11
REVIEW
Sen Xie, Yahua Lan, Chao Sun, Yongqi Shao
Insecta is the most diverse and largest class of animals on Earth, appearing together with the emergence of the first terrestrial ecosystem. Owing to this great diversity and long-term coexistence, an amazing variety of symbiotic microorganisms have adapted specifically to insects as hosts. Insect symbionts not only participate in many relationships with the hosts but also represent a novel resource for biotechnological applications. The exploitation of mutualistic symbiosis represents a promising area to search for bioactive compounds and new enzymes for potential clinical, industrial or environmental applications...
January 21, 2019: World Journal of Microbiology & Biotechnology
https://read.qxmd.com/read/30664968/a-systematic-reconsideration-on-proteases
#12
REVIEW
Poorani Gurumallesh, Kamalini Alagu, Baskar Ramakrishnan, Shanmugaprakash Muthusamy
Proteases are a group of large complex enzyme molecules that perform highly focused proteolysis functions. A vast quantity of the protease enzymes is predominantly sourced from microbial fermentation process, although proteases tend to natively present in plant, animals and humans. Proteases possess a pervasive importance in medical and pharmaceutical sector, because of its enriched specificity towards biomolecules. They are also actively encompassed in regulating certain physiological pathways. A distinct territory of human disorders is treated by substrate specific proteases...
January 18, 2019: International Journal of Biological Macromolecules
https://read.qxmd.com/read/30592890/-development-of-biotechnology-of-fermented-milk-product-with-lactobacillus-reuteri-lr1-and-the-evaluation-of-its-functional-property-in-experiment-in-vitro-and-in-vivo
#13
V F Semenikhina, I V Rozhkova, A V Begunova, T V Fedorova, T I Shirshova
In this article the biotechnology of the dairy product based on the probiotic strain of Lactobacillus reuteri LR1 is presented. The following conditions of milk fermentation were screened: fermentation by monoculture of Lactobacillus reuteri LR1, fermentation by monoculture of Lactobacillus reuteri LR1 with addition of yeast extract as growth-promoting factor, and combined fermentation by Lactobacillus reuteri LR1, Lactobacillus helveticus NK1 and Streptococcus thermophilus (HTC). It had been demonstrated that after 8 hours of cultivation the number of Lactobacillus reuteri LR1 cells in the monoculture with introduced yeast extract was up to 5,9×108 CFU/cm3 whereas cell count for the monoculture without yeast extract introduction was 1,6×107 CFU/cm3 ...
2018: Voprosy Pitaniia
https://read.qxmd.com/read/30584707/-new-concept-animal-vaccines-emerging-in-recent-years
#14
Tengteng Zhang, Yuzi Luo, Yuan Sun, Taiyuan Li, Hua-Ji Qiu
Animal infectious diseases pose a serious and continuing threat to the animal health and cause huge economic losses throughout the world. Vaccination is one of the most effective solutions to prevent and control animal infectious diseases. With the development of biotechnologies and the need for disease prevention and control, the focus of vaccine research has been shifted to the development of safe, efficient, broad-spectrum, low-dose and marker vaccines. Novel vaccines capable of inducing high levels of both humoral and cellular immune responses are promising to provide more efficient protection against animal infectious diseases...
December 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://read.qxmd.com/read/30583706/the-use-of-terpenes-and-derivatives-as-a-new-perspective-for-cardiovascular-disease-treatment-a-patent-review-2008-2018
#15
Eric Aian P Silva, Jéssica S Carvalho, Adriana G Guimarães, Rosana de S S Barreto, Márcio R V Santos, André S Barreto, Lucindo J Quintans-Júnior
Terpenes are a class of secondary metabolites that can be found in a variety of animal and plants species. They are considered the most structurally diversified and abundant of all natural compounds. Several studies have shown the application of terpenes, such as carvacrol, linalool, and limonene in many pharmaceutical and medicinal fields, including cardiovascular disorders, the leading cause of death worldwide. Areas covered: In this review, the authors outlined patents from the last 10 years relating to the therapeutic application of terpenes for the treatment and/or prevention of cardiovascular diseases found in different databases, emphasizing the possibility of these compounds becoming new drugs that may help to decrease the burden of these disorders...
December 24, 2018: Expert Opinion on Therapeutic Patents
https://read.qxmd.com/read/30582408/modulation-of-autophagy-and-protease-activities-by-small-bioactive-compounds-to-reduce-cell-death-and-improve-stress-induced-microspore-embryogenesis-initiation-in-rapeseed-and-barley
#16
Yolanda Pérez-Pérez, Ivett Bárány, Eduardo Berenguer, Elena Carneros, María C Risueño, Pilar S Testillano
Microspore embryogenesis is a powerful biotechnological tool that is very useful in crop breeding for the rapid production of haploid and double-haploid embryos and plants. In this in vitro system, the haploid microspore is reprogrammed by the application of specific stress treatments. A high level of cell death after the stress is a major factor that greatly reduces embryogenesis yield at its initial stages. Autophagy is a degradation pathway that is present in all eukaryotes and plays key roles in a range of processes, including stress responses...
December 22, 2018: Plant Signaling & Behavior
https://read.qxmd.com/read/30579706/-species-diversity-of-non-tuberculous-mycobacteria-isolated-from-aquatic-environments-of-general-pico-city-province-of-la-pampa-argentina
#17
Claudia A Tortone, Delia S Oriani, Ana S Staskevich, Alejandra S Oriani, Lilia M Gino, María J Marfil, Alejandro Nava Vargas, Andrea K Gioffré, Martín J Zumárraga
Non-tuberculous mycobacteria (NTM) are studied not only for their importance as emerging opportunistic pathogens but also for their applications in biotechnology and bioremediation. Our aim was to determine the occurrence and diversity of mycobacteria in different aquatic habitats of General Pico city, Province of La Pampa. The percentage of samples with positive cultures for mycobacteria were the following: 37.5% recovered from the water supply distribution system; 32.6% from the aquifer that supplies water to the distribution system; 36...
December 19, 2018: Revista Argentina de Microbiología
https://read.qxmd.com/read/30577419/polyamines-in-microalgae-something-borrowed-something-new
#18
REVIEW
Hung-Yun Lin, Han-Jia Lin
Microalgae of different evolutionary origins are typically found in rivers, lakes, and oceans, providing more than 45% of global primary production. They provide not only a food source for animals, but also affect microbial ecosystems through symbioses with microorganisms or secretion of some metabolites. Derived from amino acids, polyamines are present in almost all types of organisms, where they play important roles in maintaining physiological functions or against stress. Microalgae can produce a variety of distinct polyamines, and the polyamine content is important to meet the physiological needs of microalgae and may also affect other species in the environment...
December 20, 2018: Marine Drugs
https://read.qxmd.com/read/30571917/microfluidic-platform-for-multimodal-analysis-of-enzyme-secretion-in-nanoliter-droplet-arrays
#19
Dominik Huemmer, Simon Bachler, Martin Köhler, Lars M Blank, Renato Zenobi, Petra S Dittrich
High-throughput screening of cell-secreted proteins is essential for various biotechnological applications. In this article, we show a microfluidic approach to perform the analysis of cell-secreted proteins in nanoliter droplet arrays by two complementary methods, fluorescence microscopy and mass spectrometry. We analyzed the secretion of the enzyme phytase, a phosphatase used as an animal feed additive, from a low number of yeast cells. Yeast cells were encapsulated in nanoliter volumes by droplet microfluidics and deposited on spatially-defined spots on the surface of a glass slide mounted on the motorized stage of an inverted fluorescence microscope...
December 20, 2018: Analytical Chemistry
https://read.qxmd.com/read/30564608/myconanotechnology-in-veterinary-sector-status-quo-and-future-perspectives
#20
REVIEW
Mai Hamed Hanafy
Nanotechnology is no longer a concept or a theory of the new world, it has turned into a new enabling technology over the years, with tremendous potential to revolutionize agriculture and livestock sector all over the globe. Moreover, nanotechnology provides new tools for molecular and cellular biology, biotechnology, veterinary physiology and reproduction, giving more promising solutions in both pathogen detection and therapy, engineering of agriculture, incredible results in animal and food systems and many more...
December 2018: International Journal of Veterinary Science and Medicine
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