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H9c2 and extract

Min Wang, Ying Liu, Rui-Le Pan, Rui-Ying Wang, Shi-Lan Ding, Wan-Rui Dong, Gui-Bo Sun, Jing-Xue Ye, Xiao-Bo Sun
Myrica rubra is well known for its delicious taste and high nutritional value. The present study investigated the potential protective effects and mechanisms of M. rubra flavonoids (MRF) extract on isoproterenol (ISO)‑induced myocardial injury in rats and hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes. An in vivo study revealed that MRF decreased serum cardiac enzyme levels, ameliorated pathological heart alterations and increased the antioxidant potential. The in vitro investigation demonstrated that MRF inhibited cell death, reactive oxygen species (ROS) accumulation, mitochondrial membrane depolarization, apoptosis rate and caspase‑3 activation and enhanced the Bcl‑2/Bax ratio during H/R injury...
March 12, 2019: International Journal of Molecular Medicine
P L Reshma, P Binu, Nair Anupama, R C Vineetha, S Abhilash, R Harikumaran Nair, K G Raghu
The aim of the present investigation is the evaluation and elucidation of the mechanisms by which Tribulus terrestris L. methanol extract (TTM) devoid of fruit exhibits protection against cardiac ischemia in in vitro (H9c2 cell line) and in vivo (Wistar rat) model. Tribulus terrestris L. (TT) was used in this study to evaluate the efficacy against cardiac ischemia employing in vitro and in vivo models of myocardial ischemia. H9c2 cells were used for the in vitro induction of ischemia. Male Wistar rats (10 weeks old) weighing 180-220 g were used for the in vivo experiments...
March 2019: Biomedicine & Pharmacotherapy
Jie Li, Wei Shi, Jun Zhang, Liqun Ren
BACKGROUND Sepsis is defined as a systemic inflammatory response syndrome caused by an infection (suspicious or confirmed). Its essence is inflammatory mediators and cytokines mediated by host immune response. The present study aimed to investigate the role of Periplaneta americana extracts (XML) on PTEN-induced kinase 1 (PINK1)/Parkin mediated mitophagy in cardiomyocyte injury by sepsis. MATERIAL AND METHODS H9C2 cells were cultured and transfected with Mdivi-1 and Atg7 siRNA. The cell viability and drug toxicity were detected using Cell Counting Kit-8 assay...
February 21, 2019: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Yuping Yang, Zijun Jiang, Dong Zhuge
Emodin is a natural product extracted from Rheum palmatum. There are few recent studies on emodin in the treatment of myocarditis. This study aimed to investigate the effect of emodin on lipopolysaccharide (LPS)-induced inflammatory injury in cardiomyocytes. H9c2 cells were treated with 10 μM of LPS and different concentrations (0, 1, 5, 10, 15, and 20 μM) of emodin. The expression of miR-223 was changed by transient transfection. Thereafter, cell viability, apoptosis, the expression of CyclinD1 and Jnk-associated proteins, and the release of pro-inflammatory factors were assessed by cell Counting Kit-8, flow cytometry analysis, quantitative real-time polymerase chain reaction Western blot, and enzyme-linked immunosorbent assay respectively...
February 8, 2019: International Heart Journal
Matteo Bauckneht, Fabio Pastorino, Patrizia Castellani, Vanessa Cossu, Anna Maria Orengo, Patrizia Piccioli, Laura Emionite, Selene Capitanio, Nikola Yosifov, Silvia Bruno, Edoardo Lazzarini, Mirco Ponzoni, Pietro Ameri, Anna Rubartelli, Silvia Ravera, Silvia Morbelli, Gianmario Sambuceti, Cecilia Marini
BACKGROUND: Oxidative stress and its interference on myocardial metabolism play a major role in Doxorubicin (DXR) cardiotoxic cascade. METHODS: Mice models of neuroblastoma (NB) were treated with 5 mg DXR/kg, either free (Free-DXR) or encapsulated in untargeted (SL[DXR]) or in NB-targeting Stealth Liposomes (pep-SL[DXR] and TP-pep-SL[DXR]). Control mice received saline. FDG-PET was performed at baseline (PET1) and 7 days after therapy (PET2). At PET2 Troponin-I and NT-proBNP were assessed...
February 8, 2019: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
Jing-Ke Zhang, Meng Li, Miao Li, Kun Du, Jinjin Lv, Zhi-Guang Zhang, Xiao-Ke Zheng, Wei-Sheng Feng
Four new C-geranyl flavonoids, paulownione D-G (1-4) were isolated from the 50% acetone-H2 O extract of the flowers of Paulownia fortunei. The structures of the compounds were determined by extensive spectroscopic analyses (UV, IR, HR-ESI-MS, 1D and 2D NMR). All of the compounds (1-4) exhibited potent protection effects in H9c2 cardiocytes against the lipopolysaccharide (LPS)-induced inflammation.
January 30, 2019: Natural Product Research
Xiaopeng Wen, Kazumi Iwata, Keiko Ikuta, Xueqing Zhang, Kai Zhu, Masakazu Ibi, Misaki Matsumoto, Nozomi Asaoka, Junjie Liu, Masato Katsuyama, Chihiro Yabe-Nishimura
The involvement of superoxide-generating NADPH oxidase (NOX) in the cytotoxic effects of cigarette smoke extracts has been documented. However, the underlying molecular mechanisms and NOX isoform involved have not been fully clarified. Among the different NADPH oxidase isoforms identified so far, NOX1 and NOX4 were found to be expressed in rat H9c2 cardiomyocytes. When H9c2 cells were exposed to acrolein or methyl vinyl ketone (MVK), major toxic components of cigarette smoke extracts, a dose-dependent decline in cell viability was observed...
January 17, 2019: FEBS Journal
J S Gui, J Jalil, Z Jubri, Y Kamisah
Parkia speciosa Hassk is a plant found abundantly in the Southeast Asia region. Its seeds, with or without pods, have been used in traditional medicine locally to treat cardiovascular problems. The pathogenesis of cardiovascular diseases involves inflammation and oxidative stress. Based on this information, we sought to investigate the potential protective effects of Parkia speciosa empty pod extract (PSE) on inflammation in cardiomyocytes exposed to tumor necrosis factor-α (TNF-α). H9c2 cardiomyocytes were divided into four groups; negative control, TNF-α, PSE + TNF-α and quercetin + TNF-α...
January 1, 2019: Cytotechnology
Xiao Feng, Ruifei Zhang, Junjun Li, Yuan Cao, Feng Zhao, Xiaolang Du, Xiaoli Gao, Lan Cao, Suyile Chen, Pengfei Tu, Xingyun Chai
BACKGROUND: Peeled stems of Syringa pinnatifolia Hemsl. (SP) have been widely used to treat extra "He-Yi" induced myocardial ischemia for hundreds of years in Inner Mongolia, China and previous result showed that intragastric pretreatment with total extract (T) of SP has a protective effect against myocardial infarction (MI). HYPOTHESIS: This study aims to describe the pharmacological investigation and chemical characterization of the major (M) and minor (N) fractions obtained from T through column chromatography fractionation on macroporous resin and to explore whether the regulatory effects were linked to the p53-mediated apoptosis pathways...
January 2019: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Hagen Klett, Lonny Jürgensen, Patrick Most, Martin Busch, Fabian Günther, Gergana Dobreva, Florian Leuschner, David Hassel, Hauke Busch, Melanie Boerries
Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1⁻160 days post cryoinjury (dpi)...
December 28, 2018: Biomolecules
Yung-Ming Chang, Marthandam Asokan Shibu, Chuan-Te Tsai, Chin-Chuan Tsai, Shu-Luan Lin, Chia-Cheng Chang, Vijaya Padma Viswanadha, Ray-Jade Chen, Julia Huei-Mei Chang, Chih-Yang Huang
Adipose-derived mesenchymal stem cells (ADMSCs) are easily accessible and are attractive mesenchymal stem cells for use in regenerative medicine; however their application is frequently restricted due to various challenges present in the environment they are administered. Therefore ADMSCs are preferably preconditioned with various stimulating factors to overcome the barriers developed in any pathological conditions. Here we used ADMSCs from rat adipose based on the abundance of positive markers and preconditioned the cells with extracts from Alpinate Oxyphyllae Fructus (AOF), a traditional Chinese herb used for antiaging, associated various health benefits...
December 4, 2018: Journal of Cellular Physiology
Kajal Chakraborty, Soumya Krishnan, Minju Joy
Amphioctopus neglectus (Family: Octopodidae) is recognised as culinary delicacy in many cultures and a common sea food item on the Mediterranean and Asian coasts. Bioassay-directed fractionation of ethyl acetate/methanol extract of A. neglectus ensued in the characterisation of four previously undescribed macrocyclic lactones (1-4). These compounds exhibited potential radical-scavenging capacities (IC50 0.95-1.73 mM) along with anti-hypertensive activities (IC50 1.12-2.34 mM) against angiotensin converting enzyme (ACE)...
November 20, 2018: Biomedicine & Pharmacotherapy
Archana G Venakatesh, Johann J Mathew, Scott Coleman, Longqiu Yang, Geoffrey L Liu, Marilyn M Li, Henry Liu
Congestive heart failure (CHF) is defined as a cardiac dysfunction leading to low cardiac output and inadequate tissue perfusion. Intravenous positive inotropes are used to increase myocardial contractility in hospitalized patients with advanced heart failure. Milrinone is a phosphodiesterase Ⅲ inhibitor and used most commonly for inotropic effect. The well-known PROMISE study investigated the effects of milrinone on mortality in patients with severe CHF, and concluded that long-term therapy with milrinone increased morbidity and mortality among patients with advanced CHF...
November 18, 2018: Journal of Biomedical Research
Yousef Faridvand, Samira Nozari, Vahid Vahedian, Nasser Safaie, Masoud Pezeshkian, Parinaz Haddadi, Mina Mamipour, Arezoo Rezaie-Nezhad, Ahmadreza Jodati, Mohammad Nouri
BACKGROUND: human Amniotic Membrane (hAM) extracts contain bioactive molecules such as growth factors and cytokines. Studies have confirmed the ability of hAM in reduction of post-operative dysfunction in patients with cardiac surgery. However, the function of Amniotic Membrane Proteins (AMPs), extracted from hAM, against hypoxia-induced H9c2 cells injury have never been investigated. In this study, we aimed to appraise the protective impact of AMPs on H9c2 cells under hypoxia condition...
January 2019: Biomedicine & Pharmacotherapy
Prathapan A, Salin Raj P, Priya Rani M, Raghu K G
Herein, we investigated the effects of B. diffusa (BDE), a well-known cardiotonic edible medicinal plant against apoptosis in Angiotensin II (Ang II)-stimulated hypertrophic cardiac cells (H9c2). The cells were analyzed for viability, markers of hypertrophy, apoptosis, and the expression of various proteins related to apoptosis. Ang II (100 nM for 48 h)-exposed H9c2 cells treated with BDE (75 μg/ml) showed a significant reduction in apoptosis (58.60%↓) compared to Ang II-alone treated cells. BDE treatment significantly reduced the up-regulation of Bax and cytosolic cytochrome-C caused by Ang II as well as reduced the degree of Ang II- induced down-regulation of Bcl-2...
December 2018: Biomedicine & Pharmacotherapy
Cheng Zheng, Jia-Feng Lin, Zhong-Hao Lin, Wei-Qian Lin, Saroj Thapa, Yuan-Zheng Lin, Hao Lian, Zhi-Rui Liu, Jia-Hui Chen, Xiao-Wei Li
With the chronic ischemia persisting after acute myocardial infarction, the accompanying low-degree inflammation and subsequent fibrosis result in progression of cardiac remodeling and heart failure. Recently, Sodium Houttuyfonate (SH), a pure compound extracted from Houttuynia cordata , has been confirmed exerting anti-inflammatory and anti-fibrotic effects under diseased situations. Here, we aimed to investigate whether SH could reverse the cardiac remodeling post-myocardial infarction by alleviating cardiac inflammation and fibrosis...
2018: Frontiers in Pharmacology
Jing Xie, Jing Xiong, Li-Sheng Ding, Li Chen, Hua Zhou, Liang Liu, Zhi-Feng Zhang, Xue-Mei Hu, Pei Luo, Lin-Sen Qing
Although herb medicines have become the major source for new drug discovery, many of them are largely under-explored due to the purity-activity relationship. Efficient identification of bioactive compounds in conventional stepwise separation and isolation has not yet been elucidated. Therefore, we proposed a new separation strategy for holism understanding of herb pharmacology using molecularly imprinted polymers (MIPs). Astragali Radix (AR), important in traditional Chinese medicine, was chosen in this study for multicomponent knockout followed by bioactivity evaluation...
November 16, 2018: Journal of Chromatography. A
Donghai Chu, Zhenqiu Zhang
Trichosanthis Pericarpium (TP) is a traditional Chinese medicine for treating cardiovascular diseases. In this study, we investigated the effects of TP aqueous extract (TPAE) on hypoxia/reoxygenation (H/R) induced injury in H9c2 cardiomyocytes and explored the underlying mechanisms. H9c2 cells were cultured under the hypoxia condition induced by sodium hydrosulfite for 30 min and reoxygenated for 4 h. Cell viability was measured by MTT assay. The amounts of LDH, NO, eNOS, and iNOS were tested by ELISA kits...
September 20, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Vincenzo Quagliariello, Raffaele Vecchione, Carmela Coppola, Chiara Di Cicco, Alberta De Capua, Giovanna Piscopo, Rolando Paciello, Viviana Narciso, Carmen Formisano, Orazio Taglialatela-Scafati, Rosario Vincenzo Iaffaioli, Gerardo Botti, Paolo Antonio Netti, Nicola Maurea
Doxorubicin is a highly active antineoplastic agent, but its clinical use is limited because of its cardiotoxicity. Although nutraceuticals endowed with anti-inflammatory properties exert cardioprotective activity, their bioavailability and stability are inconsistent. In an attempt to address this issue, we evaluated whether bioavailable nanoemulsions loaded with nutraceuticals (curcumin and fresh and dry tomato extracts rich in lycopene) protect cardiomyoblasts (H9C2 cells) from doxorubicin-induced toxicity...
September 14, 2018: Nutrients
Wei Zhao, Yongwei Wang, Guanshan Wei, Shiyuan Xu
OBJECTIVE: To investigate the role of poly(ADP-ribose) polymerases-1 (PARP-1)-mediated blockade of autophagic flow in myocardial ischemia-reperfusion injury. METHODS: H9c2 cells, a rat cardiac myocyte line, were divided into control group, hypoxia/ reoxygenation model group (H/R group), PARP-1 inhibitor (PJ34) group, and PJ34 + H/R group. The total protein was extracted from the cells in each group to detect the expressions of pADPr, Bax, the DNA damage marker protein p-YH2ax, and autophagic flow-associated proteins LC3BⅡ/LC3Ⅰ, Beclin-1, and P62 using Western blotting...
July 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
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