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Cardiomyocytes and extract

Min Wang, Ying Liu, Rui-Le Pan, Rui-Ying Wang, Shi-Lan Ding, Wan-Rui Dong, Gui-Bo Sun, Jing-Xue Ye, Xiao-Bo Sun
Myrica rubra is well known for its delicious taste and high nutritional value. The present study investigated the potential protective effects and mechanisms of M. rubra flavonoids (MRF) extract on isoproterenol (ISO)‑induced myocardial injury in rats and hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes. An in vivo study revealed that MRF decreased serum cardiac enzyme levels, ameliorated pathological heart alterations and increased the antioxidant potential. The in vitro investigation demonstrated that MRF inhibited cell death, reactive oxygen species (ROS) accumulation, mitochondrial membrane depolarization, apoptosis rate and caspase‑3 activation and enhanced the Bcl‑2/Bax ratio during H/R injury...
March 12, 2019: International Journal of Molecular Medicine
A Ballini, S Cantore, S Scacco, L Perillo, A Scarano, S K Aityan, M Contaldo, K Cd Nguyen, L Santacroce, J Syed, D De Vito, G Dipalma, C Gargiulo Isacco, F Inchingolo
OBJECTIVE: The clinical use of mesenchymal stem cells (MSCs) in regenerative medicine either in tissue repair or tissue reconstruction has given highly interesting results thanks to their particular nature. Sources that have attracted the attention of medical scientists from where stem cells (SCs) in adults could be obtained are different and, dental tissues have certainly become an optimal source of MSCs. Dental tissue is a main reservoir of two types of MSCs dental bud (DBSCs) that constitute the immature precursor of the tooth and dental pulp (DPSCs) that are derived from dental inner pulp and partly from dental follicle tissue and can differentiate into several cell phenotypes as osteoblast, chondrocyte, hepatocytes, cardiomyocytes, neuron and β cells...
February 2019: European Review for Medical and Pharmacological Sciences
InKyu Moon, Ezat Ahmadzadeh, Keyvan Jaferzadeh, Namgon Kim
This paper investigates the rhythm strip and parameters of synchronization of human induced pluripotent stem cell (iPS) derived cardiomyocytes. The synchronization is evaluated from quantitative phase images of beating cardiomyocytes which are obtained using the time-lapse digital holographic imaging method. By quantitatively monitoring the dry mass redistribution, digital holography provides the physical contraction-relaxation signal caused by autonomous cardiac action potential. In order to analyze the synchronicity at the cell-to-cell level, we extracted single cardiac muscle cells, which contain the nuclei, from the phase images of cardiomyocytes containing multiple cells resulting from the fusion of k-means clustering and watershed segmentation algorithms...
February 1, 2019: Biomedical Optics Express
Jie Li, Wei Shi, Jun Zhang, Liqun Ren
BACKGROUND Sepsis is defined as a systemic inflammatory response syndrome caused by an infection (suspicious or confirmed). Its essence is inflammatory mediators and cytokines mediated by host immune response. The present study aimed to investigate the role of Periplaneta americana extracts (XML) on PTEN-induced kinase 1 (PINK1)/Parkin mediated mitophagy in cardiomyocyte injury by sepsis. MATERIAL AND METHODS H9C2 cells were cultured and transfected with Mdivi-1 and Atg7 siRNA. The cell viability and drug toxicity were detected using Cell Counting Kit-8 assay...
February 21, 2019: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Alexis Bouin, Paul-Antoine Gretteau, Michel Wehbe, Fanny Renois, Yohan N'Guyen, Nicolas Leveque, Michelle N Vu, Steven Tracy, Nora M Chapman, Patrick Bruneval, Paul Fornes, Bert L Semler, Laurent Andreoletti
BACKGROUND: Group B enteroviruses are common causes of acute myocarditis which can be a precursor of chronic myocarditis and dilated cardiomyopathy (DCM), leading causes of heart transplantation. To date, the specific viral functions involved in the development of DCM remain unclear. METHODS: Total RNA from cardiac tissue of patients suffering from DCM was extracted, and sequences corresponding to the 5' termini of enterovirus RNAs were identified. Following NexGen RNA sequencing, viral cDNA clones mimicking the enterovirus RNA sequences found in patient tissues were generated in vitro, and their replication and impact on host cell functions were assessed on primary human cardiac cells in culture...
February 13, 2019: Circulation
Yuping Yang, Zijun Jiang, Dong Zhuge
Emodin is a natural product extracted from Rheum palmatum. There are few recent studies on emodin in the treatment of myocarditis. This study aimed to investigate the effect of emodin on lipopolysaccharide (LPS)-induced inflammatory injury in cardiomyocytes. H9c2 cells were treated with 10 μM of LPS and different concentrations (0, 1, 5, 10, 15, and 20 μM) of emodin. The expression of miR-223 was changed by transient transfection. Thereafter, cell viability, apoptosis, the expression of CyclinD1 and Jnk-associated proteins, and the release of pro-inflammatory factors were assessed by cell Counting Kit-8, flow cytometry analysis, quantitative real-time polymerase chain reaction Western blot, and enzyme-linked immunosorbent assay respectively...
February 8, 2019: International Heart Journal
Ranabir Sahu, Tarun K Dua, Sonjit Das, Vincenzo De Feo, Saikat Dewanjee
The present investigation has been undertaken to reveal the protective mechanism of polyphenolics extract of whole wheat grains (WWGPE), ferulic acid and apigenin against doxorubicin (Dox)-induced cardio-toxicity. WWGPE, apigenin, and ferulic acid exhibited concentration dependent cyto-protective effect against Dox (1 μM) in rat cardiomyocytes. Dox treatment significantly (p < 0.01) induced oxidative stress in the myocardial cells via excessive ROS production, increase in iNOS expression, NADPH oxidase activation, Nrf-2/HO-1 impairment, and inactivation of cellular redox defense system...
February 5, 2019: Food and Chemical Toxicology
Jessica L Cao, Stephanie M Adaniya, Michael W Cypress, Yuta Suzuki, Yoichiro Kusakari, Bong Sook Jhun, Jin O-Uchi
Recent discoveries of the molecular identity of mitochondrial Ca2+ influx/efflux mechanisms have placed mitochondrial Ca2+ transport at center stage in views of cellular regulation in various cell-types/tissues. Indeed, mitochondria in cardiac muscles also possess the molecular components for efficient uptake and extraction of Ca2+ . Over the last several years, multiple groups have taken advantage of newly available molecular information about these proteins and applied genetic tools to delineate the precise mechanisms for mitochondrial Ca2+ handling in cardiomyocytes and its contribution to excitation-contraction/metabolism coupling in the heart...
March 15, 2019: Archives of Biochemistry and Biophysics
Xiaopeng Wen, Kazumi Iwata, Keiko Ikuta, Xueqing Zhang, Kai Zhu, Masakazu Ibi, Misaki Matsumoto, Nozomi Asaoka, Junjie Liu, Masato Katsuyama, Chihiro Yabe-Nishimura
The involvement of superoxide-generating NADPH oxidase (NOX) in the cytotoxic effects of cigarette smoke extracts has been documented. However, the underlying molecular mechanisms and NOX isoform involved have not been fully clarified. Among the different NADPH oxidase isoforms identified so far, NOX1 and NOX4 were found to be expressed in rat H9c2 cardiomyocytes. When H9c2 cells were exposed to acrolein or methyl vinyl ketone (MVK), major toxic components of cigarette smoke extracts, a dose-dependent decline in cell viability was observed...
January 17, 2019: FEBS Journal
Min Yi, Hekai Li, Xiaoya Wang, Jianyun Yan, Long Gao, Yinyan He, Xinglong Zhong, Yanbin Cai, Weijing Feng, Zhanpeng Wen, Chengtie Wu, Caiwen Ou, Jiang Chang, Minsheng Chen
Although numerous therapies are widely applied clinically and stem cells and/or biomaterial based in situ implantations have achieved some effects, few of these have observed robust myocardial regeneration. The beneficial effects on cardiac function and structure are largely acting through paracrine signaling, which preserve the border-zone around the infarction, reduce apoptosis, blunt adverse remodeling, and promote angiogenesis. Ionic extracts from biomaterials have been proven to stimulate paracrine effects and promote cell-cell communications...
January 9, 2019: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Ardo Illaste, Marcel Wullschleger, Miguel Fernandez-Tenorio, Ernst Niggli, Marcel Egger
Analysis of Ca2+ signals obtained in various cell types (i.e., cardiomyocytes) is always a tradeoff between acquisition speed and signal/noise ratio of the fluorescence signal. This becomes especially apparent during fast two- or three-dimensional confocal imaging when local intracellular fluorescence signals originating from Ca2+ release from intracellular Ca2+ stores (e.g., sarcoplasmic reticulum) need to be examined. Mathematical methods have been developed to remedy a high noise level by fitting each pixel with a transient function to "denoise" the image...
December 25, 2018: Biophysical Journal
J S Gui, J Jalil, Z Jubri, Y Kamisah
Parkia speciosa Hassk is a plant found abundantly in the Southeast Asia region. Its seeds, with or without pods, have been used in traditional medicine locally to treat cardiovascular problems. The pathogenesis of cardiovascular diseases involves inflammation and oxidative stress. Based on this information, we sought to investigate the potential protective effects of Parkia speciosa empty pod extract (PSE) on inflammation in cardiomyocytes exposed to tumor necrosis factor-α (TNF-α). H9c2 cardiomyocytes were divided into four groups; negative control, TNF-α, PSE + TNF-α and quercetin + TNF-α...
January 1, 2019: Cytotechnology
Xiao Feng, Ruifei Zhang, Junjun Li, Yuan Cao, Feng Zhao, Xiaolang Du, Xiaoli Gao, Lan Cao, Suyile Chen, Pengfei Tu, Xingyun Chai
BACKGROUND: Peeled stems of Syringa pinnatifolia Hemsl. (SP) have been widely used to treat extra "He-Yi" induced myocardial ischemia for hundreds of years in Inner Mongolia, China and previous result showed that intragastric pretreatment with total extract (T) of SP has a protective effect against myocardial infarction (MI). HYPOTHESIS: This study aims to describe the pharmacological investigation and chemical characterization of the major (M) and minor (N) fractions obtained from T through column chromatography fractionation on macroporous resin and to explore whether the regulatory effects were linked to the p53-mediated apoptosis pathways...
January 2019: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Hagen Klett, Lonny Jürgensen, Patrick Most, Martin Busch, Fabian Günther, Gergana Dobreva, Florian Leuschner, David Hassel, Hauke Busch, Melanie Boerries
Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1⁻160 days post cryoinjury (dpi)...
December 28, 2018: Biomolecules
Zheng Zhao, Yun Zhao, Li Ying-Chun, Lei Zhao, Wei Zhang, Jian-Guo Yang
Ischemia-reperfusion (I/R) injury often leads to myocardial apoptosis and necrosis. Studies have demonstrated the role microRNAs (miRs) played in myocardial I/R injury. Thus, we established a myocardial I/R injury model and a thoracic epidural anesthesia (TEA) model in mice to explore whether microRNA-374 (miR-374) affects myocardial I/R injury. We collected myocardial tissues to evaluate whether TEA exerts a protection effect on myocardial tissues. In addition, the levels of miR-374, dystrobrevin alpha (DTNA), and the statue of the Notch1 axis were detected...
November 22, 2018: Journal of Cellular Physiology
Kyle Bruun, Erika Schermer, Anjali Sivendra, Emily Valaik, Reed B Wise, Rana Said, John R Bracht
Cardiovascular disease (CVD) is the number one cause of death globally, and new therapeutic techniques outside of traditional pharmaceutical and surgical interventions are currently being developed. At the forefront is stem cell-centered therapy, with adipose derived stem cells (ADSCs), an adult stem population, providing significant clinical promise. When introduced into damaged heart tissue, ADSCs promote cardiac regeneration by a variety of mechanisms including differentiation into new cardiomyocytes and secretion of paracrine factors acting on endogenous cardiac cells...
2018: American Journal of Stem Cells
Hao Liu, Xuechun Chen, Xiaoping Zhao, Buchang Zhao, Ke Qian, Yang Shi, Mirko Baruscotti, Yi Wang
Wenxin Keli (WXKL) is a widely used Chinese botanical drug for the treatment of arrhythmia, which is consisted of four herbs and amber. In the present study, we analyzed the chemical composition of WXKL using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) to tentatively identify 71 compounds. Through typical separate procession, the total extract of WXKL was divided into fractions for further bioassays. Cardiomyocytes and zebrafish larvae were applied for assessment. In vivo arrhythmia model in Cmlc2-GFP transgenic zebrafish was induced by terfenadine, which exhibited obvious reduction of heart rate and occurrence of atrioventricular block...
2018: Frontiers in Pharmacology
Wenqian Fang, Aina He, Mei-Xiang Xiang, Yan Lin, Yajun Wang, Jie Li, Chongzhe Yang, Xian Zhang, Cong-Lin Liu, Galina K Sukhova, Natasha Barascuk, Lise Larsen, Morten Karsdal, Peter Libby, Guo-Ping Shi
BACKGROUND: Extracellular matrix metabolism and cardiac cell death participate centrally in myocardial infarction (MI). This study tested the roles of collagenolytic cathepsin K (CatK) in post-MI left ventricular remodeling. METHODS AND RESULTS: Patients with acute MI had higher plasma CatK levels (20.49 ± 7.07 pmol/L, n = 26) than those in subjects with stable angina pectoris (8.34 ± 1.66 pmol/L, n = 28, P = .01) or those without coronary heart disease (6...
November 19, 2018: Journal of Molecular and Cellular Cardiology
Qiuping Wu, Qianhao Zhao, Kun Yin, Bing-Jie Hu, Jianding Cheng
Sudden unexplained nocturnal death syndrome (SUNDS) is widely considered to be related to hereditary fatal arrhythmias. Hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4) channels are widely distributed in sinus myocytes and play a profound role in generating pacemaker electro-activity in cardiomyocytes. In the present study, the potential correlation between HCN4 gene variations and the occurrence of SUNDS was investigated. Genomic DNA was extracted from blood samples of both 119 unrelated SUNDS patients and 184 healthy individuals and screened for candidate HCN4 gene variants...
November 19, 2018: Journal of Forensic Sciences
Archana G Venakatesh, Johann J Mathew, Scott Coleman, Longqiu Yang, Geoffrey L Liu, Marilyn M Li, Henry Liu
Congestive heart failure (CHF) is defined as a cardiac dysfunction leading to low cardiac output and inadequate tissue perfusion. Intravenous positive inotropes are used to increase myocardial contractility in hospitalized patients with advanced heart failure. Milrinone is a phosphodiesterase Ⅲ inhibitor and used most commonly for inotropic effect. The well-known PROMISE study investigated the effects of milrinone on mortality in patients with severe CHF, and concluded that long-term therapy with milrinone increased morbidity and mortality among patients with advanced CHF...
November 18, 2018: Journal of Biomedical Research
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