keyword
https://read.qxmd.com/read/38529192/brucine-entrapped-titanium-oxide-nanoparticle-for-anticancer-treatment-an-in-vitro-study
#21
JOURNAL ARTICLE
Rashed M Almuqbil
Backgroundand Objective . The public's health has been seriously threatened by cervical cancer during recent times. In terms of newly diagnosed cases worldwide, it ranks as the ninth most prevalent malignancy. Multiple investigations have proven that nanoparticles can effectively combat cancer due to their small dimensions and extensive surface area. In the meantime, bioactive compounds which are biocompatible are being loaded onto nanoparticles to promote cancer therapy. The current study investigates the anticancerous potential of Brucine-entrapped titanium oxide nanoparticles (TiO2 NPs) in cervical cancer cell line (HeLa)...
2024: Advances in pharmacological and pharmaceutical sciences
https://read.qxmd.com/read/38528911/a-case-report-of-pallister-killian-syndrome-with-an-unusual-mosaic-supernumerary-marker-chromosome-12-with-interstitial-12p13-1-p12-1-duplication
#22
JOURNAL ARTICLE
T V Karamysheva, I N Lebedev, L I Minaycheva, L P Nazarenko, A A Kashevarova, D A Fedotov, N A Skryabin, M E Lopatkina, A D Cheremnykh, E A Fonova, T V Nikitina, E A Sazhenova, M M Skleimova, N A Kolesnikov, G V Drozdov, Y S Yakovleva, G N Seitova, K E Orishchenko, N B Rubtsov
Pallister-Killian syndrome (PKS) is a rare inherited disease with multiple congenital anomalies, profound intellectual disability, and the presence in the karyotype of sSMC - i(12)(p10). The frequency of PKS may be underestimated due to problems with cytogenetic diagnosis caused by tissue-specific mosaicism and usually a low percentage of peripheral blood cells containing sSMC. Such tissue-specific mosaicism also complicates a detailed analysis of the sSMC, which, along with the assessment of mosaicism in different tissues, is an important part of cytogenetic diagnosis in PKS...
2024: Frontiers in Genetics
https://read.qxmd.com/read/38528852/aberrant-glycosylation-in-pancreatic-ductal-adenocarcinoma-3d-organoids-is-mediated-by-kras-mutations
#23
JOURNAL ARTICLE
Hiromitsu Nakahashi, Tatsuya Oda, Osamu Shimomura, Yoshimasa Akashi, Kazuhiro Takahashi, Yoshihiro Miyazaki, Tomoaki Furuta, Yukihito Kuroda, Pakavarin Louphrasitthiphol, Bryan J Mathis, Hiroaki Tateno
Aberrant glycosylation in tumor cells is a hallmark during carcinogenesis. KRAS gene mutations are the most well-known oncogenic abnormalities but their association with glycan alterations in pancreatic ductal adenocarcinoma (PDAC) is largely unknown. We employed patient-derived 3D organoids to culture pure live PDAC cells, excluding contamination by fibroblasts and immune cells, to gasp the comprehensive cancer cell surface glycan expression profile using lectin microarray and transcriptomic analyses. Surgical specimens from 24 PDAC patients were digested and embedded into a 3D culture system...
2024: Journal of Oncology
https://read.qxmd.com/read/38528729/indole-3-acetic-acid-alleviates-dss-induced-colitis-by-promoting-the-production-of-r-equol-from-bifidobacterium-pseudolongum
#24
JOURNAL ARTICLE
Miaomiao Li, Xue Han, Lijun Sun, Xinjuan Liu, Weizhen Zhang, Jianyu Hao
BACKGROUND: Inflammatory bowel disease (IBD) is characterized by immune-mediated, chronic inflammation of the intestinal tract. The occurrence of IBD is driven by the complex interactions of multiple factors. The objective of this study was to evaluate the therapeutic effects of IAA in colitis. METHOD: C57/BL6 mice were administered 2.5% DSS in drinking water to induce colitis. IAA, Bifidobacterium pseudolongum, and R-equol were administered by oral gavage and fed a regular diet...
2024: Gut Microbes
https://read.qxmd.com/read/38528708/strong-prevalence-of-light-regime-specific-qtl-in-arabidopsis-detected-using-automated-high-throughput-phenotyping-in-fluctuating-or-constant-light
#25
JOURNAL ARTICLE
Marc C Heuermann, Rhonda C Meyer, Dominic Knoch, Henning Tschiersch, Thomas Altmann
Plants have evolved and adapted under dynamic environmental conditions, particularly to fluctuating light, but plant research has often focused on constant growth conditions. To quantitatively asses the adaptation to fluctuating light, a panel of 384 natural Arabidopsis thaliana accessions was analyzed in two parallel independent experiments under fluctuating and constant light conditions in an automated high-throughput phenotyping system upgraded with supplemental LEDs. While the integrated daily photosynthetically active radiation was the same under both light regimes, plants in fluctuating light conditions accumulated significantly less biomass and had lower leaf area during their measured vegetative growth than plants in constant light...
2024: Physiologia Plantarum
https://read.qxmd.com/read/38528705/exosomal-circ_0000735-contributes-to-non-small-lung-cancer-malignant-progression
#26
JOURNAL ARTICLE
Sijie Liu, Xun Wu, Yanjie Wang, Yingtai Chen
Circular RNA is an important regulator for non-small cell lung cancer (NSCLC). Circ_0000735 has been found to be significantly overexpressed in NSCLC tissues. Therefore, its role and mechanism in NSCLC progression need to be further explored. The expression levels of circ_0000735, miR-345-5p and A disintegrin and metalloprotease 19 (ADAM19) were determined using quantitative real-time PCR. EdU staining, wound healing and transwell assays were utilized to detect cell proliferation and metastasis. The protein levels of metastasis markers, exosome markers and ADAM19 were determined using western blot...
April 2024: Journal of Biochemical and Molecular Toxicology
https://read.qxmd.com/read/38528654/cd44-and-cd133-protein-expression-might-serve-as-a-prognostic-factor-for-early-occurrence-castration-resistant-prostate-cancer
#27
JOURNAL ARTICLE
Yayi Dwina, Litta Septina Mahmelia Zaid, Meilania Saraswati, Lisnawati Rachmadi, Aria Kekalih, Nur Rahadiani, Melva Louisa, Hasrayati Agustina, Chaidir Arif Mochtar, Agus Rizal Ardy Hariandy Hamid
BACKGROUND: The occurrence of castration-resistant prostate cancer (CRPC) varies in patients with advanced prostate cancer (PCa) undergoing androgen deprivation therapy (ADT). The rate of occurrence of CRPC may be related to the presence of prostate cancer stem cells (CSC). Thus, this study aims to evaluate the presence of CSC markers (CD44 and CD133) in histopathology tissue at the time of diagnosis and their correlation with the occurrence of CRPC in patients with advanced PCa within 2 years of ADT...
March 25, 2024: Prostate
https://read.qxmd.com/read/38528641/low-expression-of-mir-182-caused-by-dna-hypermethylation-accelerates-acute-lymphocyte-leukemia-development-by-targeting-pbx3-and-bcl2-mir-182-promoter-methylation-is-a-predictive-marker-for-hypomethylation-agents%C3%A2-%C3%A2-bcl2-inhibitor-venetoclax
#28
JOURNAL ARTICLE
Danyang Li, Yigang Yuan, Chen Meng, Zihan Lin, Min Zhao, Liuzhi Shi, Min Li, Daijiao Ye, Yue Cai, Xiaofei He, Haige Ye, Shujuan Zhou, Haixia Zhou, Shenmeng Gao
BACKGROUND: miR-182 promoter hypermethylation frequently occurs in various tumors, including acute myeloid leukemia, and leads to low expression of miR-182. However, whether adult acute lymphocyte leukemia (ALL) cells have high miR-182 promoter methylation has not been determined. METHODS: To assess the methylation status of the miR-182 promoter, methylation and unmethylation-specific PCR analysis, bisulfite-sequencing analysis, and MethylTarget™ assays were performed to measure the frequency of methylation at the miR-182 promoter...
March 26, 2024: Clinical Epigenetics
https://read.qxmd.com/read/38528626/potential-ocular-indicators-to-distinguish-posterior-cortical-atrophy-and-typical-alzheimer-s-disease-a-cross-section-study-using-optical-coherence-tomography-angiography
#29
JOURNAL ARTICLE
Yan Sun, Lumi Zhang, Hui Ye, Lumin Leng, Yi Chen, Yujie Su, Peifang Ren, Hong Lu, Guoping Peng
BACKGROUND: Posterior cortical atrophy (PCA) is a form of dementia that frequently displays significant visual dysfunction and relatively preserved cognitive and executive functions, thus hindering early diagnosis and treatment. This study aimed to investigate possible fundus markers in PCA patients and compare them with those of typical Alzheimer's disease (AD) patients to seek potential diagnostic patterns. METHODS: Age-matched PCA and AD patients and healthy controls (HC) completed optometry, intraocular pressure measurement, neuropsychologic assessments, optical coherence tomography (OCT), and optical coherence tomography angiography (OCTA) examination in one visit...
March 25, 2024: Alzheimer's Research & Therapy
https://read.qxmd.com/read/38528189/identification-of-hdac9-and-arrdc4-as-potential-biomarkers-and-targets-for-treatment-of-type-2-diabetes
#30
JOURNAL ARTICLE
Jing Liu, Lingzhen Meng, Zhihong Liu, Ming Lu, Ruiying Wang
We aimed to identify the key potential insulin resistance (IR)-related genes and investigate their correlation with immune cell infiltration in type 2 diabetes (T2D). The GSE78721 dataset (68 diabetic patients and 62 controls) was downloaded from the Gene Expression Omnibus database and utilized for single-sample gene set enrichment analysis. IR-related genes were obtained from the Comparative Toxicology Genetics Database, and the final IR-differentially expressed genes (DEGs) were screened by intersecting with the DEGs obtained from the GSE78721 datasets...
March 25, 2024: Scientific Reports
https://read.qxmd.com/read/38528186/assessing-gpt-4-for-cell-type-annotation-in-single-cell-rna-seq-analysis
#31
JOURNAL ARTICLE
Wenpin Hou, Zhicheng Ji
Here we demonstrate that the large language model GPT-4 can accurately annotate cell types using marker gene information in single-cell RNA sequencing analysis. When evaluated across hundreds of tissue and cell types, GPT-4 generates cell type annotations exhibiting strong concordance with manual annotations. This capability can considerably reduce the effort and expertise required for cell type annotation. Additionally, we have developed an R software package GPTCelltype for GPT-4's automated cell type annotation...
March 25, 2024: Nature Methods
https://read.qxmd.com/read/38528069/ikk2-controls-the-inflammatory-potential-of-tissue-resident-regulatory-t-cells-in-a-murine-gain-of-function-model
#32
JOURNAL ARTICLE
Chelisa Cardinez, Yuwei Hao, Kristy Kwong, Ainsley R Davies, Morgan B Downes, Nadia A Roberts, Jason D Price, Raquel A Hernandez, Jessica Lovell, Rochna Chand, Zhi-Ping Feng, Anselm Enders, Carola G Vinuesa, Bahar Miraghazadeh, Matthew C Cook
Loss-of-function mutations have provided crucial insights into the immunoregulatory actions of Foxp3+ regulatory T cells (Tregs). By contrast, we know very little about the consequences of defects that amplify aspects of Treg function or differentiation. Here we show that mice heterozygous for an Ikbkb gain-of-function mutation develop psoriasis. Doubling the gene dose (IkbkbGoF/GoF ) results in dactylitis, spondylitis, and characteristic nail changes, which are features of psoriatic arthritis. IkbkbGoF mice exhibit a selective expansion of Foxp3 + CD25+ Tregs of which a subset express IL-17...
March 25, 2024: Nature Communications
https://read.qxmd.com/read/38528037/comparative-response-to-pdt-with-methyl-aminolevulinate-and-temoporfin-in-cutaneous-and-oral-squamous-cell-carcinoma-cells
#33
JOURNAL ARTICLE
J Nicolás-Morala, M Alonso-Juarranz, A Barahona, S Terrén, S Cabezas, F Falahat, Y Gilaberte, S Gonzalez, A Juarranz, M Mascaraque
Cutaneous and Head and Neck squamous cell carcinoma (CSCC, HNSCC) are among the most prevalent cancers. Both types of cancer can be treated with photodynamic therapy (PDT) by using the photosensitizer Temoporfin in HNSCC and the prodrug methyl-aminolevulinate (MAL) in CSCC. However, PDT is not always effective. Therefore, it is mandatory to correctly approach the therapy according to the characteristics of the tumour cells. For this reason, we have used cell lines of CSCC (A431 and SCC13) and HNSCC (HN5 and SCC9)...
March 25, 2024: Scientific Reports
https://read.qxmd.com/read/38527989/the-importance-of-combined-hpv-and-cintec%C3%A2-plus-genotyping-testing-for-p16-in-women-with-cervical-squamous-cell-carcinoma
#34
JOURNAL ARTICLE
Toader Septimiu Voidăzan, Mihaela Alexandra Budianu, Ovidiu Simion Cotoi, Sabin Gligore Turdean
INTRODUCTION: Immunohistochemistry (IHC) for p16INK4A (p16) is a reliable surrogate test for the presence of a high-risk, potentially transformative human papillomavirus (HPV) infection in precursor and malignant lesions of the cervix. The purpose of this study was to evaluate changes in cervical cells caused by persistent HPV infection, by IHC (p16 protein) by comparison with HPV genotyping. PATIENTS, MATERIALS AND METHODS: The study included female patients aged between 26 and 57 years who presented to a public hospital, with complaints related to the genital area, namely vaginal bleeding and dyspareunia...
2024: Romanian Journal of Morphology and Embryology
https://read.qxmd.com/read/38527980/immunoexpression-of-ki67-p16-and-beta-catenin-in-precursor-lesions-of-cutaneous-squamous-cell-carcinoma
#35
JOURNAL ARTICLE
Alina Maria Vîlcea, Loredana Elena Stoica, Bianca Cătălina Andreiana, Cecil Sorin Mirea, Tiberiu Ştefăniţă Ţenea Cojan, Ioana Cristina Vîlcea, Claudiu Mărgăritescu
Cutaneous squamous cell carcinoma (CSCC) is the second most common type of skin cancer, after basal cell carcinoma, representing about 10-20% of all malignant skin tumors. The mortality rates of CSCC approach those of renal and oropharyngeal carcinomas, as well as melanoma, with the increasing of the risk once metastases and perineural invasion occur. Both actinic keratosis (AK) and Bowen's disease (BD) are direct precursors with the potential for progression to CSCC. In this study, we analyzed the expression of Ki67, P16 and Beta-catenin in the precursor lesions of CSCC in relation to histological prognostic parameters, respectively between them, with the aim of identifying possible correlations with a role in prognosis...
2024: Romanian Journal of Morphology and Embryology
https://read.qxmd.com/read/38527908/status-of-b-lymphocyte-subsets-and-their-homing-markers-in-patients-with-post-kala-azar-dermal-leishmaniasis
#36
JOURNAL ARTICLE
Shilpa Sengupta, Deep Goswami, Bidhan Chakraborty, Surya Jyati Chaudhuri, Manab K Ghosh, Mitali Chatterjee
In visceral leishmaniasis, the Type II helper T cell predominance results in B cell modulation and enhancement of anti-leishmanial IgG. However, information regarding its dermal sequel, post-kala-azar dermal leishmaniasis (PKDL), remains limited. Accordingly, this study aimed to elucidate the B cell-mediated antibody-dependent/independent immune profiles of PKDL patients. In the peripheral blood of PKDL patients, immunophenotyping of B cell subsets was performed by flow cytometry and by immunohistochemistry at lesional sites...
March 2024: Parasite Immunology
https://read.qxmd.com/read/38527668/an-improved-mouse-model-of-sepsis-based-on-intraperitoneal-injections-of-the-enriched-culture-of-cecum-slurry
#37
JOURNAL ARTICLE
Rajat Atre, Rahul Sharma, Alexander G Obukhov, Uzma Saqib, Sadiq Umar, Gajanan N Darwhekar, Mirza S Baig
AIM: Sepsis is a life-threatening clinical syndrome comprising multiorgan dysfunctions caused by a disproportionate body immune response. There are several animal sepsis models which are based on cecum ligation, cecal puncture, and cecum slurry injection. The major limitation of all current sepsis models is the high variability owing to the variable degree of ligation, puncture and inconsistent microbial composition used for sepsis initiation. The primary objective of this work is to demonstrate the feasibility of a standardized method for sepsis development...
March 23, 2024: Life Sciences
https://read.qxmd.com/read/38527567/shikonin-attenuates-cerebral-ischemia-reperfusion-injury-via-inhibiting-nod2-rip2-nf-%C3%AE%C2%BAb-mediated-microglia-polarization-and-neuroinflammation
#38
JOURNAL ARTICLE
Ya Yang, Yuxiang Fei, Xuejiao Xu, Jun Yao, Jianing Wang, Chao Liu, Haiyan Ding
OBJECTIVES: Microglia-mediated neuroinflammation plays a crucial role in the pathophysiological process of multiple neurological disorders such as ischemic stroke, which still lacks effective therapeutic agents. Shikonin possesses anti-inflammatory and neuroprotective properties. However, its underlying mechanism remains elusive. This study aimed to investigate whether Shikonin confers protection against cerebral ischemia/reperfusion (I/R) injury by modulating microglial polarization and elucidate the associated mechanisms...
March 23, 2024: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://read.qxmd.com/read/38527517/establishment-and-validation-of-an-efficient-method-for-the-3d-culture-of-osteoclasts-in-vitro
#39
JOURNAL ARTICLE
Abdullah Faqeer, Jie Liu, Li Zhang, Changde Wang, Guangqian Zhou, Yang Zhang
INTRODUCTION: Osteoclasts (OCs) play a crucial role in maintaining bone health. Changes in OC activity are linked to different bone diseases, making them an intriguing focus for research. However, most studies on OCs have relied on 2D cultures, limiting our understanding of their behavior. Yet, there's a lack of knowledge regarding platforms that effectively support osteoclast formation in 3D cultures. METHODS: In our investigation, we explored the capacity of collagen and GelMA hydrogels to facilitate osteoclast development in 3D culture settings...
March 23, 2024: Journal of Dentistry
https://read.qxmd.com/read/38527431/characterization-of-pleural-mesothelioma-by-hierarchical-clustering-analyses-using-immune-cells-within-tumor-microenvironment
#40
JOURNAL ARTICLE
Shingo Inaguma, Chengbo Wang, Sunao Ito, Akane Ueki, Jerzy Lasota, Piotr Czapiewski, Renata Langfort, Janusz Rys, Joanna Szpor, Piotr Waloszczyk, Krzysztof Okoń, Wojciech Biernat, Shuji Takiguchi, David S Schrump, Markku Miettinen, Satoru Takahashi
INTRODUCTION: Over the past decade, classifications using immune cell infiltration have been applied to many types of tumors; however, mesotheliomas have been less frequently evaluated. METHODS: In this study, 60 well-characterized pleural mesotheliomas (PMs) were evaluated immunohistochemically for the characteristics of immune cells within tumor microenvironment (TME) using 10 immunohistochemical markers CD3, CD4, CD8, CD56, CD68, CD163, FOXP3, CD27, PD-1 and TIM-3...
March 25, 2024: Pathobiology: Journal of Immunopathology, Molecular and Cellular Biology
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