Kevin Ullmann, Felix Manstein, Wiebke Triebert, Nils Kriedemann, Annika Franke, Jana Teske, Mira Mertens, Victoria Lupanow, Gudrun Göhring, Alexandra Haase, Ulrich Martin, Robert Zweigerdt
BACKGROUND: Human pluripotent stem cells (hPSCs) have an enormous therapeutic potential, but large quantities of cells will need to be supplied by reliable, economically viable production processes. The suspension culture (three-dimensional; 3D) of hPSCs in stirred tank bioreactors (STBRs) has enormous potential for fuelling these cell demands. In this study, the efficient long-term matrix-free suspension culture of hPSC aggregates is shown. METHODS AND RESULTS: STBR-controlled, chemical aggregate dissociation and optimized passage duration of 3 or 4 days promotes exponential hPSC proliferation, process efficiency and upscaling by a seed train approach...
March 25, 2024: Stem Cell Research & Therapy