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3D morphology evaluation

Julia Herzen, Dimitrios C Karampinos, Peter Foehr, Lorenz Birnbacher, Manuel Viermetz, Rainer Burgkart, Thomas Baum, Fabian Lohoefer, Moritz Wildgruber, Franz Schilling, Marian Willner, Mathias Marschner, Peter B Noël, Ernst J Rummeny, Franz Pfeiffer, Pia M Jungmann
OBJECTIVE: Aim of this study was, to demonstrate the feasibility of high-resolution grating-based X-ray phase-contrast computed tomography (PCCT) for quantitative assessment of cartilage. MATERIALS AND METHODS: In an experimental setup, 12 osteochondral samples were harvested from n = 6 bovine knees (n = 2 each). From each knee, one cartilage sample was degraded using 2.5% Trypsin. In addition to PCCT and biomechanical cartilage stiffness measurements, 3T and 7T MRI was performed including MSME SE T2 and ME GE T2* mapping sequences for relaxationtime measurements...
2019: PloS One
Yutao Jian, Li Dao, Xiaodong Wang, Xinping Zhang, Michael V Swain, Ke Zhao
OBJECTIVE: To identify the conditions under which fabrication pore defects within veneering porcelain in bilayered lithium disilicate glass-ceramic (LDG) crowns will influence and jeopardize the mechanical integrity of the structure. METHODS: Thirty standardized molar crowns (IPS e.max Press) were fabricated and microCT scanned to 3D-analyze the size, morphology and distribution of pores in veneering porcelain, followed by in vitro fracture test and SEM fractographic observation...
February 8, 2019: Dental Materials: Official Publication of the Academy of Dental Materials
Munkhbaatar Batmunkh, Svetlana V Aksenova, Lkhagvaa Bayarchimeg, Aleksandr N Bugay, Oidov Lkhagva
The study of evaluating radiation risk on the central nervous system induced by space-born charged particles is very complex and challenging task in space radiobiology and radiation protection. To overcome computational difficulties in this field, we developed simplified neuron models with properties equivalent to realistic neuron morphology. Three-dimensional structure and parameters of simplified and complex neuron models with realistic morphology were obtained from the experimental data. The models implement uniform random distribution of spines along the dendritic branches in typical hippocampal neurons...
January 2019: Physica Medica: PM
Nikolay Dukov, Kristina Bliznakova, Firgan Feradov, Ivan Buliev, Hilde Bosmans, Giovanni Mettivier, Paolo Russo, Lesley Cockmartin, Zhivko Bliznakov
This paper presents a method for creation of computational models of breast lesions with irregular shapes from patient Digital Breast Tomosynthesis (DBT) images or breast cadavers and whole-body Computed Tomography (CT) images. The approach includes six basic steps: (a) normalization of the intensity of the tomographic images; (b) image noise reduction; (c) binarization of the lesion area, (d) application of morphological operations to further decrease the level of artefacts; (e) application of a region growing technique to segment the lesion; and (f) creation of a final 3D lesion model...
January 2019: Physica Medica: PM
Tara McCray, Zachary Richards, Joseph Marsili, Gail S Prins, Larisa Nonn
This paper describes a detailed protocol for three-dimensional (3D) culturing, handling, and evaluation of human primary prostate organoids. The process involves seeding of epithelial cells sparsely in a 3D matrix gel on a 96-well microplate with media changes to cultivate expansion into organoids. Morphology is then assessed by whole-well capturing of z-stack images. Compression of z-stacks creates a single in-focus image from which organoids are measured to quantify a variety of outputs, including circularity, roundness, and area...
January 17, 2019: Journal of Visualized Experiments: JoVE
Silmi Mariya, Fitriya N Dewi, Irma H Suparto, Gregory K Wilkerson, J Mark Cline, Diah Iskandriati, I Nengah Budiarsa, Dondin Sajuthi
The mammary gland contains adult stem cells that are capable of self-renewal. Although these cells hold an important rolein the biology and pathology of the breast, the studies of mammary stem cells are few due to the difficulty of acquiring andexpanding undifferentiated adult stem cell populations. In this study, we developed mammosphere cultures from frozen mammary cells of nulliparous cynomolgus macaques (Macaca fascicularis) as a culture system to enrich mammary stem cells. Small samples of mammary tissues were collected by surgical biopsy; cells were cultured in epithelial cell growth medium and cryopreserved...
February 7, 2019: Comparative Medicine
Akiko Narita, Yuki Morizane, Tomoe Miyake, Kae Sugihara, Tomoko Ishikawa, Jiro Seguchi, Fumio Shiraga
PURPOSE: To identify the cross-sectional morphological changes in successful filtering blebs after phacoemulsification using swept-source three-dimensional anterior segment optical coherence tomography (3D AS-OCT). MATERIALS AND METHODS: Thirty phakic eyes of 29 patients with successful filtering blebs after primary trabeculectomy were included in this retrospective cohort study. Success was defined as intraocular pressure (IOP)≤15▒mmHg and a>20% reduction in IOP without glaucoma medication or additional glaucoma surgery after trabeculectomy...
February 1, 2019: Journal of Glaucoma
Tomoki Ohori, Tatsuo Mae, Konsei Shino, Yuta Tachibana, Hideki Yoshikawa, Ken Nakata
BACKGROUND: Three-dimensional (3D) computed tomography (CT) is reliable and accurate imaging modality for evaluating tunnel enlargement after anterior cruciate ligament (ACL) reconstruction. The purpose of this study was to evaluate the tibial tunnel enlargement including the morphological change after anatomic ACL reconstruction with a bone-patellar tendon-bone (BTB) graft using 3D CT models. METHODS: Eighteen patients with unilateral ACL rupture were included...
January 29, 2019: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
A Fuchs, A Youssef, A Seher, G Hochleitner, P D Dalton, S Hartmann, R C Brands, U D A Müller-Richter, C Linz
BACKGROUND: The spectrum of indications for the use of membranes and scaffolds in the field of oral and maxillofacial surgery includes, amongst others, guided bone regeneration (GBR). Currently available membrane systems face certain disadvantages such as difficult clinical handling, inconsistent degradation, undirected cell growth and a lack of stability that often complicate their application. Therefore, new membranes which can overcome these issues are of great interest in this field...
February 1, 2019: BMC Oral Health
Xin Che, Han Wu, Changkai Jia, Huimin Sun, Shangkun Ou, Junqi Wang, M Vimalin Jeyalatha, Xin He, Jingwen Yu, Chengyou Zuo, Zuguo Liu, Wei Li
Purpose: To investigate a novel strategy in constructing tissue-engineered corneal stromal equivalent based on amniotic membrane and keratocytes. Methods: The ultrathin amniotic membrane (UAM) was laminated, with corneal stromal cells (CSCs) distributed between the space of the layered UAMs. Calcein AM staining was used to evaluate cellular viability, morphology, and arrangement. Immunostaining, qRT-PCR, and Western blot were performed to detect gene and protein expression in keratocytes...
February 1, 2019: Investigative Ophthalmology & Visual Science
Sandro Queirós, Pedro Morais, Wolfgang Fehske, Alexandros Papachristidis, Jens-Uwe Voigt, Jaime C Fonseca, Jan D'hooge, João L Vilaça
The assessment of aortic valve (AV) morphology is paramount for planning transcatheter AV implantation (TAVI). Nowadays, pre-TAVI sizing is routinely performed at one cardiac phase only, usually at mid-systole. Nonetheless, the AV is a dynamic structure that undergoes changes in size and shape throughout the cardiac cycle, which may be relevant for prosthesis selection. Thus, the aim of this study was to present and evaluate a novel software tool enabling the automatic sizing of the AV dynamically in three-dimensional (3D) transesophageal echocardiography (TEE) images...
January 30, 2019: International Journal of Cardiovascular Imaging
Makoto Suruga, Takashi Horaguchi, Takanori Iriuchishima, Genki Iwama, Yoshiyuki Yahagi, Yasuaki Tokuhashi, Shin Aizawa
BACKGROUNDS: "Anatomical" anterior cruciate ligament (ACL) reconstruction is defined as the functional restoration of the ACL to its native dimensions. It is essential to obtain more accurate predictors of ACL size before surgery. The purpose of this study was to investigate the correlation between the native femoral ACL footprint size and the morphology of the distal femur using three-dimensional CT (3D-CT). METHODS: Thirty non-paired Japanese human cadaver knees were used...
January 31, 2019: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
Andrea C Jimenez-Vergara, Rodrigo Zurita, Abigail Jones, Patricia Diaz Rodroguez, Xin Qu, Kenneth L Kusima, Mariah S Hahn, Dany J Munoz-Pinto
Numerous studies have demonstrated that the differentiation potential of human mesenchymal stem cells (hMSCs) can be modulated by chemical and physical cues. In 2D contexts, inducing different cell morphologies by varying the shape, area and/or curvature of adhesive islands on patterned surfaces has significant effects on hMSC multipotency and the onset of differentiation. In contrast, in vitro studies in 3D contexts have suggested that hMSC differentiation does not directly correlate with cell shape. However, in 3D, the effects of cell morphology on hMSC differentiation has not yet been clearly established due to the chemical and physical properties being intertwined in 3D matrices...
January 25, 2019: Acta Biomaterialia
Vandna Dhanwal, Archana Katoch, Amanpreet Singh, Souneek Chakraborty, Mir Mohd Faheem, Gaganpreet Kaur, Debasis Nayak, Narinder Singh, Anindya Goswami, Navneet Kaur
Organic nanoparticles (ONPs) possess great research interests for their promising effects in the enhancement of bioactivity including anticancer activity with less toxicity. The present study describes the preparation, characterization and biological evaluation of aqueous phase ONPs of potent 1,2-disubstituted benzimidazole derivative (BZ6) for anticancer activity. BZ6-ONPs were characterized through UV-absorption and fluorescence spectroscopic analysis for their photo-physical properties. DLS, TEM and SEM studies were carried out for morphological and structural analysis...
April 2019: Materials Science & Engineering. C, Materials for Biological Applications
Masanori Yoshino, Taichi Kin, Takayuki Hara
BACKGROUND: Successful resection of arteriovenous malformation (AVM) depends on preoperative assessment of the detailed morphology of the AVM. Simultaneous detailed three-dimensional (3D) visualization of the feeding arteries, draining veins, and surrounding structures is needed. In OBJECTIVE: Tthe present study, we aimed to evaluated the usefulness of high-resolution 3D multi-fusion medical imaging (hr-3DMMI) for preoperative planning of AVM. METHODS: Hr-3DMMI combined magnetic resonance imaging (MRI), MR angiography, thin-slice computed tomography, and 3D rotated angiography...
January 21, 2019: World Neurosurgery
Patrick J Denard, Matthew T Provencher, Alexandre Lädermann, Anthony A Romeo, Bradford O Parsons, Joshua S Dines
Background: Our purpose was to compare the output of glenoid measurements with 2 commercially available preoperative 3-dimensional (3D) total shoulder arthroplasty planning systems. The hypothesis was that there would be no difference in product-derived measurements between the systems. Methods: Preoperative 3D computed tomography scans of 63 consecutive patients undergoing primary arthroplasty were analyzed using 2 product-derived techniques: Blueprint and VIP...
December 2018: JSES open access
Zhi-Jun Zhao, Min Gao, SoonHyoung Hwang, Sohee Jeon, Inkyu Park, Sanghu Park, Jun-Ho Jeong
A novel concept for fabricating heterogeneous nanostructures based on different melting temperatures is developed. Au-Ag composite cross structures are fabricated by nanowelding technologies. During the fabrication of Au-Ag composite cross structures, Ag nanowires transform into ordered particles decorating the Au nanowire surfaces with an increase in welding temperature because of the different melting temperatures of Au and Ag. To compare and explain the melting temperatures, the thicknesses of Au and Ag nanowires as parameters are analyzed...
January 23, 2019: ACS Applied Materials & Interfaces
Hui Zhao, Chang Yan, Yibing Hu, Lei Mu, Kaiyu Huang, Qiling Li, Xiaolan Li, Deding Tao, Jichao Qin
Three‑dimensional (3D) cultures are indispensable for capturing tumor heterogeneity in colorectal cancer (CRC) in vitro. Although 3D cultures (such as sphere‑forming assay and organoid culture) can partially preserve the morphological and molecular characteristics of primary CRC, whether these 3D cultures maintain the long‑term stemness of cancer stem cells (CSCs) remains largely unknown. In the present study, spheres and organoids were generated side by side using individual primary CRC specimens, then respectively processed as serial passages...
January 11, 2019: International Journal of Oncology
Qing Li, Xiongxin Lei, Xiaofei Wang, Zhigang Cai, Peijun Lyu, Guifeng Zhang
3D printing provides a novel approach to repair bone defects using customized biomimetic tissue scaffolds. To make a bone substitute closest to natural bone structure and composition, two different types of hydroxyapatite, Nano hydroxyapatite (nHA) and deproteinized bovine bone (DBB), were dispersed into collagen (CoL) to prepare the bio-ink for 3D printing. In doing so, a porous architecture was manufactured with 3D printing technology. The physical and chemical properties of the materials were evaluated, including biocompatibility and effect on the osteogenic differentiation of the human bone marrow-derived mesenchyme stem cells (hBMSCs)...
January 16, 2019: Tissue Engineering. Part A
Ryunosuke Oikawa, Goro Tajima, Jun Yan, Moritaka Maruyama, Atsushi Sugawara, Shinya Oikawa, Takaaki Saigo, Hirotaka Takahashi, Minoru Doita
BACKGROUND: To clarify, with three-dimensional (3D) images, the morphological properties of the patellar tendon and both of its insertion sites. METHODS: Thirty-two human cadaveric left knees were evaluated, and 3D computed tomography images were created. These images were used to analyse the morphology of both insertion sites of the patellar tendon, and the width, length and thickness of each region of the patellar tendon. RESULTS: The insertion sites of the patellar tendon on the patellar and tibial sides were V-shaped and crescent-shaped, respectively, with the respective bony apexes located at 44...
January 8, 2019: Knee
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