Read by QxMD icon Read


Adrian Schmalen, Julia Karius-Fischer, Pia Rauch, Christian Setz, Klaus Korn, Petra Henklein, Torgils Fossen, Ulrich Schubert
As part of the Pr55Gag polyprotein, p6 fulfills an essential role in the late steps of the replication cycle. However, almost nothing is known about the functions of the mature HIV-1 p6 protein. Recently, we showed that p6 is a bona fide substrate of the insulin-degrading enzyme (IDE), a ubiquitously expressed zinc metalloprotease. This phenomenon appears to be specific for HIV-1, since p6 homologs of HIV-2, SIV and EIAV were IDE-insensitive. Furthermore, abrogation of the IDE-mediated degradation of p6 reduces the replication capacity of HIV-1 in an Env-dependent manner...
December 12, 2018: Viruses
Philipp Karius, Alexander Lembcke, Felix C Sokolowski, Ivan Dario Perez Gandara, Alejandra Rodríguez, Bernd Hamm, Marc Dewey
OBJECTIVE: To analyse extracardiac findings in patients without significant coronary artery disease (CAD) in general and in symptomatic patients in particular. METHODS: We searched the Radiology Information System database for coronary computed tomography angiographies (CTA) performed from 2000-2014 and retrospectively enrolled 3,898 patients without significant CAD (coronary stenosis < 50%) in CTA. In 2,330 symptomatic patients, we analysed the spectrum of extracardiac findings and identified pathologies potentially explaining chest pain...
September 25, 2018: European Radiology
Florin Eggmann, Julia Bühler, Tatjana Ochsner, Roland Weiger, Clemens Walter
Karius and Baktus, first published in 1949, is one of the most influential storybooks with dental caries as main theme and it remains in wide use for domestic health education. This study aimed at assessing oral health related contents of the historic storybook in the light of the current body of cariologic evidence. Two investigators independently identified phrases in the storybook with cariologically relevant content. Dental health related contents were systematically evaluated with respect to the current body of cariologic evidence...
December 0: Journal of the History of Dentistry
Felix C Sokolowski, Philipp Karius, Alejandra Rodríguez, Alexander Lembcke, Moritz Wagner, Bernd Hamm, Marc Dewey
OBJECTIVES: To determine the prevalence and significance of extracardiac findings (ECF) in a large set of cardiac magnetic resonance (MR) imaging examinations. METHODS: The institutional review board (IRB) of the Charité approved this retrospective, single-centre study. A total of 4376 cardiac MR imaging reports of 3553 patients (age 37.4 ± 20 years, 60.8 % male) examined from 2000 to 2014 were included. Findings with a recommendation for follow-up were considered "major ECF"...
October 2018: European Radiology
Karen Legler, Ricarda Rosprim, Tosca Karius, Kathrin Eylmann, Maila Rossberg, Ralph M Wirtz, Volkmar Müller, Isabell Witzel, Barbara Schmalfeldt, Karin Milde-Langosch, Leticia Oliveira-Ferrer
BACKGROUND: Alterations in protein glycosylation have been related to malignant transformation and tumour progression. We recently showed that low mRNA levels of Golgi alpha-mannosidase MAN1A1 correlate with poor prognosis in breast cancer patients. METHODS: We analysed the role of MAN1A1 on a protein level using western blot analysis (n=105) and studied the impact of MAN1A1-related glycosylation on the prognostic relevance of adhesion molecules involved in breast cancer using microarray data (n=194)...
March 20, 2018: British Journal of Cancer
Friedrich Hahn, Adrian Schmalen, Christian Setz, Melanie Friedrich, Stefan Schlößer, Julia Kölle, Robert Spranger, Pia Rauch, Kirsten Fraedrich, Tatjana Reif, Julia Karius-Fischer, Ashok Balasubramanyam, Petra Henklein, Torgils Fossen, Ulrich Schubert
There is a significantly higher risk for type II diabetes in HIV-1 carriers, albeit the molecular mechanism for this HIV-related pathology remains enigmatic. The 52 amino acid HIV-1 p6 Gag protein is synthesized as the C-terminal part of the Gag polyprotein Pr55. In this context, p6 promotes virus release by its two late (L-) domains, and facilitates the incorporation of the viral accessory protein Vpr. However, the function of p6 in its mature form, after proteolytic release from Gag, has not been investigated yet...
2017: PloS One
T Karius, C Deborre, D C Wirtz, C Burger, A Prescher, A Fölsch, K Kabir, R Pflugmacher, H Goost
INTRODUCTION: PMMA-augmentation of pedicle screws strengthens the bone-screw-interface reducing cut-out risk. Injection of fluid cement bears a higher risk of extravasation, with difficulty of application because of inconsistent viscosity and limited injection time. OBJECTIVE: To test a new method of cement augmentation of pedicle screws using radiofrequency-activated PMMA, which is suspected to be easier to apply and have less extravasations. METHODS: Twenty-seven fresh-frozen human cadaver lumbar spines were divided into 18 osteoporotic (BMD ≤ 0...
2017: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
Christina M Colvin, Diana Karius, Nancy M Albert
BACKGROUND: Chemotherapy medications place nurses at risk for occupational exposure, a primary nursing safety concern. No literature was available on adherence to following chemotherapy handling practices and nurses' perceptions of safe-handling practices. OBJECTIVES: The aims of the pilot study were to examine actual and subjective ambulatory oncology nurse adherence to chemotherapy safe-handling guideline recommendations that prevent chemotherapy exposure. METHODS: A prospective, comparative mixed-methods study was used to compare objective and subjective nurse behaviors of expected safe chemotherapy handling-specifically, micro-ethnography and questionnaires...
December 1, 2016: Clinical Journal of Oncology Nursing
Esther I Bernhofer, Rose Hosler, Diana Karius
Providing optimal pain care for patients is essential to the work of nursing and a measure of patient satisfaction prompting some hospitals to offer pain management classes for clinicians. Although nurses generally do well on knowledge tests after attending a pain class, actual improvement in pain care for patients may not occur. The personal values of the clinician may be a key driver of pain-management decision making. Therefore, a segment on how clinicians' personal values influence pain care decisions was added to a large Midwestern hospital's pain management class...
December 2016: Pain Management Nursing: Official Journal of the American Society of Pain Management Nurses
Samantha H Weed-Pfaff, Benjamin Nutter, James F Bena, Jennifer Forney, Rosemary Field, Lynn Szoka, Diana Karius, Patti Akins, Christina M Colvin, Nancy M Albert
BACKGROUND: A seven-item cancer-specific fall risk tool (Cleveland Clinic Capone-Albert [CC-CA] Fall Risk Score) was shown to have a strong concordance index for predicting falls; however, validation of the model is needed. OBJECTIVES: The aims of this study were to validate that the CC-CA Fall Risk Score, made up of six factors, predicts falls in patients with cancer and to determine if the CC-CA Fall Risk Score performs better than the Morse Fall Tool. METHODS: Using a prospective, comparative methodology, data were collected from electronic health records of patients hospitalized for cancer care in four hospitals...
October 1, 2016: Clinical Journal of Oncology Nursing
Jan Kosinski, Alexander von Appen, Alessandro Ori, Kai Karius, Christoph W Müller, Martin Beck
Structural characterization of large multi-subunit protein complexes often requires integrating various experimental techniques. Cross-linking mass spectrometry (XL-MS) identifies proximal protein residues and thus is increasingly used to map protein interactions and determine the relative orientation of subunits within the structure of protein complexes. To fully adapt XL-MS as a structure characterization technique, we developed Xlink Analyzer, a software tool for visualization and analysis of XL-MS data in the context of the three-dimensional structures...
March 2015: Journal of Structural Biology
Valérie Cappuyns, Valentine Deweirt, Sandra Rousseau
A possible solution for the oversupply of dredged sediments is their use as a raw material in brick production. Despite the fact that several examples (e.g., Agostini et al., 2007; Hamer and Karius, 2002; Xu et al., 2014) show that this application is feasible, some economic, technical and social limitations interfere with the development of a market of dredged materials in brick production in Flanders. While we describe the main characteristics of the supply side, we focus on the limitations and barriers from the demand side in the present study...
April 2015: Waste Management
Michael Schnekenburger, Tommy Karius, Marc Diederich
Glutathione S-transferases (GSTs) are phase II drug detoxifying enzymes that play an essential role in the maintenance of cell integrity and protection against DNA damage by catalyzing the conjugation of glutathione to a wide variety of exo- and endogenous electrophilic substrates. Glutathione S-transferase P1 (GSTP1), the gene encoding the pi-class GST, is frequently inactivated by acquired somatic CpG island promoter hypermethylation in multiple cancer subtypes including prostate, breast, liver, and blood cancers...
2014: Frontiers in Pharmacology
Cindy Grandjenette, Michael Schnekenburger, Tommy Karius, Jenny Ghelfi, Anthoula Gaigneaux, Estelle Henry, Mario Dicato, Marc Diederich
Increased proliferation rates as well as resistance to apoptosis are considered major obstacles for the treatment of patients with chronic myelogenous leukemia (CML), thus highlighting the need for novel therapeutic approaches. Since senescence has been recognized as a physiological barrier against tumorigenesis, senescence-based therapy could represent a new strategy against CML. DNA demethylating agent 5-aza-2'-deoxycytidine (DAC) was reported to induce cellular senescence but underlying mechanisms remain to be elucidated...
June 2014: Neoplasia: An International Journal for Oncology Research
Philipp Karius, Georg M Schuetz, Peter Schlattmann, Marc Dewey
UNLABELLED: Coronary computed tomography angiography (CCTA) is of growing importance in noninvasive diagnosis of coronary artery diseases. The CT data allow evaluation not only of coronary arteries but also of adjacent anatomical territories. Our objective was to review, to analyze, and to quantify the spectrum and the prevalence of extracardiac findings (ECF) in CCTA. Therefore, we searched MEDLINE, EMBASE, and ISI Web of Science. Prior to quantitative analysis, we categorized the ECF of all included studies into clinically significant and clinically non-significant findings...
May 2014: Journal of Cardiovascular Computed Tomography
Ruth Powazki, Declan Walsh, Brenda Cothren, Lisa Rybicki, Shirley Thomas, Gloria Morgan, Diana Karius, Mellar P Davis, Shiva Shrotriya
BACKGROUND: Care of the dying is a significant component of nursing practice particularly in hospitals. Nurses who work in certain areas like oncology, intensive care unit (ICU) face the care of the dying, more so than other units. OBJECTIVES: The survey was conducted to assess nurses' self-perception of their professional capability and comfort in the care of the actively dying. Determine if professional capability and comfort was associated with any of the six demographics characteristics (age, gender, clinical experience, education level, nursing unit, continuing education)...
September 2014: American Journal of Hospice & Palliative Care
Julia Arand, David Spieler, Tommy Karius, Miguel R Branco, Daniela Meilinger, Alexander Meissner, Thomas Jenuwein, Guoliang Xu, Heinrich Leonhardt, Verena Wolf, Jörn Walter
The enzymatic control of the setting and maintenance of symmetric and non-symmetric DNA methylation patterns in a particular genome context is not well understood. Here, we describe a comprehensive analysis of DNA methylation patterns generated by high resolution sequencing of hairpin-bisulfite amplicons of selected single copy genes and repetitive elements (LINE1, B1, IAP-LTR-retrotransposons, and major satellites). The analysis unambiguously identifies a substantial amount of regional incomplete methylation maintenance, i...
June 2012: PLoS Genetics
H Goost, K Kabir, D C Wirtz, C Deborre, T Karius, R Pflugmacher, E M W Koch, C Burger, C Fölsch
BACKGROUND: The anchorage of pedicle screws can be challenging in the osteoporotic spine. A promising technique are cement augumented pedicle screws. The goal of this study was to gain more information regarding application of pedicle screw augmentation in Germany. MATERIALS AND METHODS: Participants of the National German Spine Congress 2009 were invited to participate in an internet-based anonymous survey regarding application of pedicle screw augmentation. The questionnaire was related to different aspects of materials and procedures for pedicle screw augmentation...
June 2012: Zeitschrift Für Orthopädie und Unfallchirurgie
Tommy Karius, Michael Schnekenburger, Mario Dicato, Marc Diederich
MicroRNAs (miRNAs) represent a class of small (21-23 nucleotides) non-coding RNAs that emerged as key post-transcriptional gene regulators, implicated in numerous physiological and pathological processes. Currently, a main focus of miRNA research is related to the roles of miRNAs in cancer development. The biogenesis and modes of action of miRNAs have not been completely elucidated; however, miRNA-mediated translational repression is involved in the regulation of almost every cellular process. Thus, pathological alterations in miRNA expression signatures are commonly associated with disease development...
June 15, 2012: Biochemical Pharmacology
Tommy Karius, Michael Schnekenburger, Jenny Ghelfi, Jörn Walter, Mario Dicato, Marc Diederich
Glutathione-S-transferase P1 (GSTP1) gene is commonly silenced by CpG island promoter hypermethylation in prostate, breast, and liver cancers. However, mechanisms leading to GSTP1 repression by promoter hypermethylation in leukemia and its relationship with pathological alterations of the chromatin structure remain poorly understood. A panel of leukemia cell lines was analyzed for their GSTP1 expression, revealing cell lines with high, moderate or no detectable GSTP1 expression. Bisulfite sequencing, methylation-specific PCR and combined bisulfite restriction analysis revealed that GSTP1 promoter was completely methylated in transcriptionally inactive RAJI and MEG-01 cell lines...
June 1, 2011: Biochemical Pharmacology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"