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citrullinated peptide antibody

Anna Scanu, Chiara Giraudo, Francesca Galuppini, Vanni Lazzarin, Gianmaria Pennelli, Stefano Sivolella, Edoardo Stellini, Francesca Oliviero, Paola Galozzi, Massimo Rugge, Roberto Stramare, Roberto Luisetto, Leonardo Punzi
This study evaluated the arthritogenic effect of lipopolysaccharide (LPS) in a mouse model of periodontal disease. Periodontitis was induced in wild-type CD1 mice by nine LPS injections (10 or 50 ng) into the maxillary mucosa. Untreated mice or injected with LPS at the tail were used as controls. Two weeks after final inoculation, mice were sacrificed to collect blood, maxilla, and paw samples. Development and progression of periodontitis and arthritis were monitored using clinical assessment, micro-computed tomography (micro-CT), ultrasound (US), and histological analysis...
February 19, 2019: Inflammation
Do Van Giap, Jae-Wan Jung, Nan-Sun Kim
Cyclic citrullinated peptide (CCP) antibody has been shown recently to be a promising marker for early detection and diagnosis of rheumatoid arthritis (RA). In order to exploit newly developed therapies for RA, early intervention is crucial in preventing irreversible joint damage. Here, we describe use of a plant expression system to produce a CCP antibody that could be used in the early diagnosis of RA. Heavy and light chain gene sequences of a CCP monoclonal antibody (CCP mAb) were cloned from the hybridoma cell (12G1) and introduced into two separate plant expression vectors under the control of the rice α-amylase 3D (RAmy3D) promoter system...
February 11, 2019: Transgenic Research
Jie Yang, Qinghe Shao, Jieyan Wu
OBJECTIVES: To study the correlation between high-frequency ultrasonography of patients with early rheumatoid arthritis (RA) and anti-cyclic citrullinated peptide (CCP) antibody. METHODS: Two wrists, 1st to 5th metacarpal phalangeal (MCP) and 1st to 5th proximal interphalangeal (PIP) joints of 53 early RA patients treated from October 2015 to October 2017 and 30 healthy subjects were examined by high-frequency ultrasonography. The thicknesses of synovial membrane, sheaths of 1st to 5th extensor tendons, flexor tendons and ulnar wrist extensor tendons were measured...
February 2019: Medicine (Baltimore)
Shuichiro Nakabo
Several autoantibodies against proteins with post-translational modifications have been detected in patients with rheumatoid arthritis (RA) and are called anti-modified protein antibodies (AMPAs). Anti-carbamylated protein antibodies (Anti-CarP Ab) are the second most vigorously researched AMPAs following anti-citrullinated protein/peptide antibodies (ACPA). Anti-CarP Ab and ACPA show cross-reactivity to some extent and frequently co-exist with each other in RA, but are two distinct antibodies. Although the diagnostic efficacy of anti-CarP Ab is inferior to that of ACPA, the diagnostic specificity of RA may improve when used in combination with ACPA and rheumatoid factor...
February 3, 2019: Immunological medicine
Kristina Forslind, Kerstin Eberhardt, Björn Svensson
OBJECTIVE: The aim of this study was to examine the occurrence of repair in a cohort of conventionally treated patients with early rheumatoid arthritis over 8 years. METHODS: There were 395 patients included in the BARFOT study having radiographs of hands and feet at inclusion, and at 1, 2, 5, and 8 years, which were chronologically scored for erosions by the Sharp/van der Heijde method. An erosion with repair was defined as an erosion that has become partially or totally filled, with or without sclerosis...
February 1, 2019: Journal of Rheumatology
Liam J O'Neil, Mariana J Kaplan
Rheumatoid arthritis (RA), a common autoimmune disease, is characterized by a highly coordinated inflammatory response that involves innate and adaptive immunity. One of the hallmarks of RA is an immune response directed at citrullinated peptides that are specifically targeted by anticitrullinated protein antibodies (ACPAs). Among the various mechanisms by which neutrophils may promote immune dysregulation in RA, their ability to extrude neutrophil extracellular traps has recently been implicated in the development of ACPAs...
January 29, 2019: Trends in Molecular Medicine
Matthieu Ribon, Sarra Seninet, Julie Mussard, Mireille Sebbag, Cyril Clavel, Guy Serre, Marie-Christophe Boissier, Luca Semerano, Patrice Decker
OBJECTIVE: Neutrophil extracellular traps (NET), produced by activated polymorphonuclear neutrophils (PMN), are supposed to play a role in the pathogenesis of rheumatoid arthritis (RA), a chronic inflammatory autoimmune disease characterized by anti-citrullinated protein antibodies (ACPA). Indeed, NET contain citrullinated autoantigens and some RA autoantibodies recognize NET. However, the mechanisms by which NET trigger or perpetuate the inflammatory process in RA are hitherto not elucidated...
January 28, 2019: Journal of Autoimmunity
Pedro Santos-Moreno, Guillermo Sánchez, Carlos Castro
We determined whether rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibody (ACPA) can predict remission or severe disability in rheumatoid arthritis (RA) patients treated with anti-tumor necrosis factor (TNF) alpha drugs.We performed a cohort study based on the clinical data from a referral center for the treatment of RA in Bogotá, Colombia, were included patients aged ≥18 years with diagnosis of RA with an active disease and for whom a treatment scheme was begun with anti-TNF alpha medication, with a minimum follow-up time of 12 months...
February 2019: Medicine (Baltimore)
I A Guseva, A V Smirnov, N V Demidova, M Yu Krylov, A S Avdeeva, E Yu Samarkina, E L Luchikhina, D E Karateev, D D Abramov, E L Nasonov
AIM: To clarify the association between HLA-DRB1 and TNFα (-308G>A) genes polymorphism and joint destruction/further progression during 12 months of the follow-up period (FUP) in patients with early (<6 months), active, predominantly antibodies to cyclic citrullinated peptide (ACCP) and rheumatoid factor (RF)-positive rheumatoid arthritis (RA) treated according to "Treat to target" strategy. MATERIALS AND METHODS: The study included 85 patients with early RA and duration of symptoms <6 months...
May 11, 2018: Terapevticheskiĭ Arkhiv
Guillaume Courbon, Mélanie Rinaudo-Gaujous, Vincent Blasco-Baque, Isabelle Auger, Robin Caire, Lambert Mijola, Laurence Vico, Stéphane Paul, Hubert Marotte
Association between periodontal disease (PD) and rheumatoid arthritis (RA) has been extensively described, but direct evidence of causal involvement of PD in RA is missing. We investigated the priming role of oral Porphyromonas gingivalis ( P. gingivalis ) in PD and subsequent RA and we assessed biomarkers of bone resorption and arthritis development in rats. METHODS : Lewis rats were orally exposed to either P. gingivalis , Prevotella intermedia or control gel for 1 month and then followed for 8 months...
January 30, 2019: Annals of the Rheumatic Diseases
Frédéric Coutant
The identification in 1998 of the main antigenic substrate recognized by autoantibodies was a dramatic turning point in our understanding of rheumatoid arthritis (RA) biology. Now, two decades later, antibodies to citrullinated peptides are viewed no longer as mere biomarkers for RA, but also as major pathophysiological factors involved in the development of bone loss and joint pain. These pathogenic effects are ascribable to abnormal autoantibody glycosylation via a pathway involving the Th17 T cells. In the future, abnormal autoantibody glycosylation may serve as a disease activity biomarker and suggest novel treatment strategies...
January 24, 2019: Joint, Bone, Spine: Revue du Rhumatisme
Yuxuan Li, Lutian Yao, Siyan Liu, Jisheng Wu, Liping Xia, Hui Shen, Jing Lu
To investigate serum interleukin (IL)- 35 levels in patients with rheumatoid arthritis (RA) and to describe the association between serum IL-35 levels and clinical parameters: erythrocyte sedimentation rate (ESR), C reactive protein (CRP), global health on Visual Analog Scale, Disease Activity Score in 28 joints based on ESR (DAS28-ESR), rheumatoid factor (RF) and anticyclic citrullinated peptide antibodies (ACPAs). The study included 129 patients with RA and 83 healthy controls. Serum IL-35 levels were detected by ELISA...
January 18, 2019: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
Emma C de Moel, Veerle F A M Derksen, Leendert A Trouw, Holger Bang, Gerard Collée, Leroy R Lard, Sofia Ramiro, Tom W J Huizinga, Cornelia F Allaart, René E M Toes, Diane van der Woude
BACKGROUND: Rheumatoid arthritis (RA) is characterized by the presence of autoantibodies like rheumatoid factor (RF), anti-cyclic citrullinated peptide-2 (anti-CCP2), and anti-carbamylated protein (anti-CarP) antibodies. It is currently unclear whether changes in autoantibody levels are associated with disease activity/treatment outcomes and whether they are modified by treatment intensity. Therefore, we determined longitudinal changes in RA-autoantibody levels, the association between these changes and activity score (DAS) and treatment outcomes, and the effect of intensity of immunosuppressive treatment on levels...
January 18, 2019: Arthritis Research & Therapy
Amir I Elshafie, Sahwa Elbagir, Mawahib I E Aledrissy, Elnour M Elagib, Musa A M Nur, Johan Rönnelid
OBJECTIVE: Anti-cyclic citrullinated peptide 2 antibodies (anti-CCP2) and rheumatoid factor (RF) in rheumatoid arthritis (RA) has been extensively assessed in industrialized countries. We investigated the diagnostic and prognostic impact of anti-CCP2 and RF isotypes in a Sudanese cross-sectional RA cohort. METHODS: Consecutive RA patients (n = 281) diagnosed according to the 1987 ACR criteria were included 2008-2010. Anti-CCP2 and RF isotypes (IgA, IgM, and IgG) were measured by enzyme immunoassay in 262 patients, with reference intervals aligned to the same diagnostic specificity as for anti-CCP2 (97...
January 17, 2019: Clinical Rheumatology
Chase S Correia, Melissa R Briones, Rong Guo, Rochella A Ostrowski
This paper is to examine the relationship between anti-cyclic citrullinated peptide (anti-CCP) antibody titers and the development of interstitial lung disease (ILD) in patients with and without rheumatoid arthritis (RA). A retrospective investigation was conducted on all adult patients tested for anti-CCP between January 1, 2007, and December 31, 2012, in a university healthcare system. Patients with specified exposures or conditions known to cause ILD were excluded. The prevalence of ILD was compared between those with and without a positive CCP...
January 15, 2019: Clinical Rheumatology
Tuhidul Islam, Amith D Naik, Yasuhiro Hashimoto, Stefano Menegatti, Ruben G Carbonell
This work presents the use of peptide ligand HWRGWV and its cognate sequences to develop affinity adsorbents that compete with Protein A in terms of binding capacity and quality of the eluted product. First, the peptide ligand was conjugated to crosslinked agarose resins (WorkBeads) at different densities and using different spacer arms. The optimization of ligand density and display resulted in values of static and dynamic binding capacity of 85 mg/mL and 65 mg/mL, respectively. A selected peptide-WorkBeads adsorbent was utilized for purifying Mabs from Chinese Hamster Ovary (CHO) cell culture supernatants...
January 4, 2019: International Journal of Molecular Sciences
Jin-Hee Kim, In Ah Choi, Joo Youn Lee, Kyoung-Hwa Kim, Sungtae Kim, Ki-Tae Koo, Tae-Il Kim, Yang-Jo Seol, Young Ku, In-Chul Rhyu, Yeong Wook Song, Yong-Moo Lee
Purpose: Periodontitis and rheumatoid arthritis (RA) share a similar inflammatory pathogenesis. Porphyromonas gingivalis ( Pg ) can induce anticyclic-citrullinated peptide autoantibodies (anti-CCP antibodies), a key factor in the development of RA. This study aimed at evaluating the relationships between the 2 diseases and identifying the clinical implications thereof, with a focus on periodontal pathogens in Korean adults. Methods: A total of 260 RA patients and 86 age- and sex-matched control patients without arthritis were enrolled in this prospective cross-sectional study...
December 2018: Journal of Periodontal & Implant Science
Nigil Haroon
What is so negative about seronegative diseases? The term seronegative arthritis can be confusing. Does this refer to seronegative rheumatoid arthritis (RA) or spondyloarthritis (SpA)? We diagnose patients with seronegative RA if the clinical picture is consistent with RA and yet they lack serum markers such as rheumatoid factor and anti-cyclic citrullinated peptide antibodies. However, for SpA the absence of RF is a common cardinal feature, despite the varied presentations. It is well known now that low titer RF is not an exclusion for SpA and we have moved away from the term 'seronegative spondyloarthritis' to SpA...
January 7, 2019: Arthritis & Rheumatology
Yudong Liu, Chenxi Liu, Liubing Li, Fengchun Zhang, Yongzhe Li, Shulan Zhang
We evaluated the clinical performance of anti-CEP-1 in a Chinese rheumatoid arthritis (RA) cohort. A total of 264 subjects were tested, including 101 RA patients, 38 juvenile idiopathic arthritis (JIA) patients, 46 disease control (DC) and 79 healthy controls (HC). The presence of anti-CEP-1 in patients with RA, JIA, DCs and HC were 61.4%, 13.2%, 15.2% and 5.1%, respectively. Anti-CCP2 demonstrated the highest positive likelihood ratio of 10.11 in the diagnosis of RA, followed by RF (8.88) and anti-CEP-1 (5...
January 3, 2019: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Pieter Meyer, Mahmood Ally, Bridget Hodkinson, Ronald Anderson, Mohammed Tikly
Background: The immunoglobulin A isotypes of anti-cyclic citrullinated peptide antibodies (ACPA) and rheumatoid factor (RF) are associated with disease severity and progression in Caucasian rheumatoid arthritis (RA) patients, as well as with genetic predisposition and tobacco use. Objectives: To compare levels of ACPA-IgA and RF-IgA with those of ACPA-IgG and cRF in a cohort of black South African RA patients and healthy controls.To investigate the relationship between IGA autoantibodies and disease severity, genetic predisposition and tobacco use...
June 2018: African Health Sciences
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