John T Lafin, Cinzia G Scarpini, Armon Amini, Bendu Konneh, Jeffrey M Howard, Thomas Gerald, Michelle Nuno, Jin Piao, Anna Savelyeva, Zhaohui Wang, Jeffrey Gagan, Liwei Jia, Cheryl M Lewis, Sarah Murray, Yun C Sawa, Vitaly Margulis, Solomon L Woldu, Douglas W Strand, Nicholas Coleman, James F Amatruda, A Lindsay Frazier, Matthew J Murray, Aditya Bagrodia
Circulating miR-371a-3p has excellent performance in the detection of viable (non-teratoma) germ cell tumor (GCT) pre-orchiectomy; however, its ability to detect occult disease is understudied. To refine the serum miR-371a-3p assay in the minimal residual disease setting we compared performance of raw (Cq) and normalized (∆Cq, RQ) values from prior assays, and validated interlaboratory concordance by aliquot swapping. Revised assay performance was determined in a cohort of 32 patients suspected of occult retroperitoneal disease...
June 29, 2023: Scientific Reports