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TNF alpha and Mesenchymal Stem Cell

Wenchao Liu, Ran Li, Jian Yin, Shenquan Guo, Yunchang Chen, Haiyan Fan, Gancheng Li, Zhenjun Li, Xifeng Li, Xin Zhang, Xuying He, Chuanzhi Duan
BACKGROUND: Activated microglia-mediated neuroinflammation has been regarded as an underlying key player in the pathogenesis of subarachnoid hemorrhage (SAH)-induced early brain injury (EBI). The therapeutic potential of bone marrow mesenchymal stem cells (BMSCs) transplantation has been demonstrated in several brain injury models and is thought to involve modulation of the inflammatory response. The present study investigated the salutary effects of BMSCs on EBI after SAH and the potential mechanism mediated by Notch1 signaling pathway inhibition...
January 15, 2019: Journal of Neuroinflammation
S T Peruzzaro, M M M Andrews, A Al-Gharaibeh, O Pupiec, M Resk, D Story, P Maiti, J Rossignol, G L Dunbar
BACKGROUND: Traumatic brain injury (TBI) is a major cause for long-term disability, yet the treatments available that improve outcomes after TBI limited. Neuroinflammatory responses are key contributors to determining patient outcomes after TBI. Transplantation of mesenchymal stem cells (MSCs), which release trophic and pro-repair cytokines, represents an effective strategy to reduce inflammation after TBI. One such pro-repair cytokine is interleukin-10 (IL-10), which reduces pro-inflammatory markers and trigger alternative inflammatory markers, such as CD163...
January 5, 2019: Journal of Neuroinflammation
Bin Fang, Ding Wang, Jiaqian Zheng, Qiushi Wei, Dongxiang Zhan, Yamei Liu, Xuesong Yang, Haibin Wang, Gang Li, Wei He, Liangliang Xu
BACKGROUND: The etiology and pathology osteonecrosis of the femoral head (ONFH) are not completely clarified. As a cytokine participating in systemic inflammation, tumor necrosis factor alpha (TNFα) has been shown to be involved in the pathogenesis of ONFH. However, the role of TNFα in ONFH is not clearly clarified. In the present study, we investigated the effects of TNFα on proliferation, angiogenesis, and osteogenic differentiation of rat bone mesenchymal stem cells (rMSCs) and the underlying mechanisms...
January 3, 2019: Stem Cell Research & Therapy
Khaled F Al-Massri, Lamiaa A Ahmed, Hanan S El-Abhar
Peripheral neuropathy is a common adverse effect observed during the use of paclitaxel (PTX) as chemotherapy. The present investigation was directed to estimate the modulatory effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on pregabalin (PGB) treatment in PTX-induced peripheral neuropathy. Neuropathic pain was induced in rats by injecting PTX (2 mg/kg, i.p) 4 times every other day. Rats were then treated with PGB (30 mg/kg/day, p.o.) for 21 days with or without a single intravenous administration of BM-MSCs...
March 15, 2019: Behavioural Brain Research
Rahim Heidari Barchi Nezhad, Fatemeh Asadi, Mohammad Reza Mirzaei, Seyyed Meysam Abtahi Froushani
Objectives: The current investigation was undertaken to evaluate the effects of 17β- estradiol (17β-ED) on the potential of the mesenchymal stem cells (MSCs) for modulation of immunity responses in an animal model of multiple sclerosis (MS). Materials and Methods: After isolation of MSCs, cells were cultured in presence of 100 nM 17β-ED for 24 hr. Modeling of experimental autoimmune encephalomyelitis (EAE) was achieved by using guinea pig spinal cord homogenate, in addition to complete Freund's adjuvant in male Wistar rats...
September 2018: Iranian Journal of Basic Medical Sciences
Yan Zhang, Wenjin Zhang, Huancheng Wang, Bo Yang
BACKGROUND: Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are considered a new and favorable source of stem cells for cell replacement-based therapy. Some microRNAs (miRNAs) have been reported to participate in the regulation of immune responses. Our aim was to investigate the effects of miR-21 on the biological characteristics, immunoregulatory properties, and potential mechanisms of hAM-MSCs. METHODS: hAM-MSCs were isolated from the placental amnion membrane of a newborn...
November 27, 2018: Genetic Testing and Molecular Biomarkers
Rundan Hong, Zhiguo Wang, Aihua Sui, Xiaoxuan Liu, Chun Fan, Sofya Lipkind, Quanchen Xu
OBJECTIVE: To examine the effects of gingival mesenchymal stem cells (GMSCs) on inflammatory macrophages upon oxidized low-density lipoprotein (ox-LDL) stimulation and evaluate therapeutic potential of GMSCs on mouse model of periodontitis associated with hyperlipidemia. METHODS: in vitro, GMSCs were co-cultured with macrophages for 48 h in the absence or presence of M1 polarizing conditions and oxidized low-density lipoprotein in the transwell system. The supernatants were collected for ELISA...
November 14, 2018: Archives of Oral Biology
Nazli Jafarzadeh, Zohreh Safari, Majid Pornour, Naser Amirizadeh, Mehdi Forouzandeh Moghadam, Majid Sadeghizadeh
Leukemic cells can impact the bone marrow niche to create a tumor-favorable microenvironment using their secreted factors. Little knowledge is available about immunosuppressive and tumor-promoting properties of chronic myeloid leukemia derived exosomes in bone marrow stromal components. We report here that K562-derived exosomes can affect the gene expression, cytokine secretion, nitric oxide (NO) production, and redox potential of bone marrow mesenchymal stem cells (BM-MSCs) and macrophages. Human BM-MSCs and mouse macrophages were treated with K562-derived exosomes...
October 14, 2018: Journal of Cellular Physiology
Wancheng Yu, Haiying Chen, Hongli Yang, Jie Ding, Peng Xia, Xu Mei, Lei Wang, Shuangfeng Chen, Chengwei Zou, Le-Xin Wang
BACKGROUND: Pulmonary arterial hypertension (PAH) is characterised by remodelling in vascular smooth muscles, and switching from contractile (differentiated) to synthetic (dedifferentiated) phenotype. This study aimed to investigate the effect of a mutated caveolin-1 (Cav1F92A ) gene from bone marrow mesenchymal stem cells (rBMSCs) on phenotypic switching in the smooth muscle cells during PAH. METHODS: Human pulmonary smooth muscle cells (HPASMCs) were treated with monocrotaline (MCT,1μM), and co-cultured with Cav1F92A gene modified rBMSCs (rBMSCs/Cav1F92A )...
September 7, 2018: Heart, Lung & Circulation
Peilin Chen, Zhigang Wu, Alex Leung, Xiaobo Chen, Euphemie Landao-Bassonga, Junjie Gao, Lianzhi Chen, Monica Zheng, Felix Yao, Hong Yang, Lars Lidgren, Brent Allan, Yinong Liu, Tao Wang, Minghao Zheng
Alveolar bone loss is a common problem that affects dental implant placement. A barrier between the bone substitute and gingiva that can prevent fibro-tissue ingrowth, bacterial infection and induce bone formation is a key factor in improving the success of alveolar ridge reconstruction. This study aims to develop a bioactive collagen barrier material for guided bone regeneration, that is coupled with anti-bacterial and anti-inflammatory properties. We have evaluated two silver coating methods and found controllable and precise coating achieved by sonication compared with sputtering...
October 2, 2018: Biomedical Materials
A B Spejo, G B Chiarotto, A D F Ferreira, D A Gomes, R S Ferreira, B Barraviera, A L R Oliveira
BACKGROUND: Treatment of spinal cord injury is dependent on neuronal survival, appropriate synaptic circuit preservation, and inflammatory environment management. In this sense, mesenchymal stem cell (MSC) therapy is a promising tool that can reduce glial reaction and provide trophic factors to lesioned neurons. METHODS: Lewis adult female rats were submitted to a unilateral ventral funiculus cut at the spinal levels L4, L5, and L6. The animals were divided into the following groups: IA (intramedullary axotomy), IA + DMEM (Dulbecco's modified Eagle's medium), IA + FS (fibrin sealant), IA + MSC (106 cells), and IA + FS + MSC (106 cells)...
August 14, 2018: Journal of Neuroinflammation
Ryo Sugiura, Shunsuke Ohnishi, Masatsugu Ohara, Marin Ishikawa, Shuichi Miyamoto, Reizo Onishi, Koji Yamamoto, Kazumichi Kawakubo, Masaki Kuwatani, Naoya Sakamoto
Mesenchymal stem cells (MSCs) represent a valuable cell source in regenerative medicine, and large numbers of MSCs can be isolated from the amnion noninvasively. Sclerosing cholangitis is a chronic cholestatic disease and characterized by progressive biliary destruction leading to cirrhosis. Many factors are involved in the development of sclerosing cholangitis; however, effective medical therapy is not established. We investigated the effects of human amnion-derived MSCs (hAMSCs) and conditioned medium (CM) obtained from hAMSC cultures in rats with sclerosing cholangitis...
2018: American Journal of Translational Research
Iman O Sherif, Dina Sabry, Azza Abdel-Aziz, Osama M Sarhan
BACKGROUND: The therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) against cisplatin-induced nephrotoxicity has been reported, however, its efficacy in gonadotoxicity still has not been addressed. Herein, we investigated the effect of BM-MSCs in cisplatin-induced testicular toxicity and its underlying mechanism of action. METHODS: Thirty male Sprague-Dawley rats were divided into a control group: injected with phosphate-buffered saline (PBS) intraperitoneal (ip), a cisplatin group: injected with a single dose of 7 mg/kg cisplatin ip to induce gonadotoxicity and a BM-MSCs group: received cisplatin ip followed by BM-MSCs injection 1 day after cisplatin...
July 18, 2018: Stem Cell Research & Therapy
F van den Akker, K R Vrijsen, J C Deddens, J W Buikema, M Mokry, L W van Laake, P A Doevendans, J P G Sluijter
Adverse remodeling after myocardial infarction (MI) is strongly influenced by T cells. Stem cell therapy after MI, using mesenchymal stem cells (MSC) or cardiomyocyte progenitor cells (CMPC), improved cardiac function, despite low cell retention and limited differentiation. As MSC secrete many factors affecting T cell proliferation and function, we hypothesized the immune response could be affected as one of the targets of stem cell therapy. Therefore, we studied the immunosuppressive properties of human BM-MSC and CMPC and their extracellular vesicles (EVs) in co-culture with activated T cells...
June 2018: Heliyon
Martina Blaschke, Regine Koepp, Christof Lenz, Jochen Kruppa, Klaus Jung, Heide Siggelkow
Background: Crohn's disease (CD) is associated with a higher prevalence of osteoporosis, a complication that is recognized as a significant cause of morbidity. Its pathogenesis is controversial, but the activity of CD is one contributing factor. Methods: We stimulated SCP-1 cells (mesenchymal stem cell line) under osteogenic conditions with serum from adult patients with CD in the symptomatic phase (SP) and in remission (R) and with control sera. Concentrations of IL-6, IL-1 beta, and TNF alpha in the sera were measured...
September 2018: Journal of Clinical & Translational Endocrinology
Yi-Jing Wang, Pan Zhao, Bing-Dong Sui, Nu Liu, Cheng-Hu Hu, Ji Chen, Chen-Xi Zheng, An-Qi Liu, Kun Xuan, Ya-Ping Pan, Yan Jin
Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients' own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention...
June 27, 2018: Experimental & Molecular Medicine
Hanan Abdelmawgoud, Asmaa Saleh
BACKGROUND: Mesenchymal stem cells (MSCs) are of increased importance because of their capacity to counteract inflammation and suppress host immune responses. OBJECTIVES: The aim of the study was to compare the effectiveness of MSCs and hematopoietic stem cells (HSCs) in the treatment of rheumatoid arthritis (RA). MATERIAL AND METHODS: Paw swelling was assessed by measuring the thickness of the hind paws using a caliper. Cytokines - interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta (TGF-β), and IL-10 - and rheumatoid factor (RF) were measured using enzyme-linked immunosorbent assay (ELISA) kits...
July 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Patrick C Baer, Jürgen M Overath, Anja Urbschat, Ralf Schubert, Benjamin Koch, Asanke A Bohn, Helmut Geiger
Stem cell-based therapies require cells with a maximum regenerative capacity in order to support regeneration after tissue injury and organ failure. Optimization of this regenerative potential of mesenchymal stromal/stem cells (MSC) or their conditioned medium by in vitro preconditioning regimens are considered to be a promising strategy to improve the release of regenerative factors. In the present study, MSC were isolated from inguinal adipose tissue (mASC) from C57BL/6 mice, cultured, and characterized. Then, mASC were either preconditioned by incubation in a hypoxic environment (0...
June 10, 2018: International Journal of Molecular Sciences
Xiaoru Xin, Chen Wang, Zhuojia Lin, Jie Xu, Yanan Lu, Qiuyu Meng, Xiaonan Li, Yuxin Yang, Qidi Zheng, Xin Gui, Tianming Li, Hu Pu, Wujun Xiong, Jiao Li, Song Jia, Dongdong Lu
Inflammatory and autophagy-related gene P62 is highly expressed in most human tumor tissues. Herein, we demonstrate that P62 promotes human mesenchymal stem cells' malignant transformation via the cascade of P62-tumor necrosis factor alpha (TNF-α)-CUDR-CTCF-insulin growth factor II (IGFII)-H-Ras signaling. Mechanistically, we reveal P62 enhances IGFII transcriptional activity through forming IGFII promoter-enhancer chromatin loop and increasing METTL3 occupancy on IGFII 3' UTR and enhances H-Ras overexpression by harboring inflammation-related factors, e...
June 1, 2018: Molecular Therapy. Nucleic Acids
Zhongfang Zhao, Ying Han, Zhihui Zhang, Wenwen Li, Xiaoli Ji, Xiaodan Liu, Jianqiu Jin, Si Xu, Hongmei Cui, Zhe Cheng, Qian Wang, Xing Wang, Xiang Guo, Yixiang Wang, Hongwei Liu
Mesenchymal stem cells (MSCs) have been used clinically and experimentally to relieve severe immune-related diseases due to their immunomodulatory properties, but these are impaired by inflammation. Oral lichen planus (OLP) is a T cell-mediated chronic inflammatory mucosal disease. In the present study, we found MSCs from OLP with higher expression of interleukin (IL)-6, tumour necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β) and IL-10 compared with control. Total glucosides of paeony (TGP) significantly improves the immunomodulatory function of MSCs by inhibiting IL-6 and TNF-α expression and increasing TGF-β and IL-10 expression...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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