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https://read.qxmd.com/read/30746843/synthetic-structural-and-rna-binding-studies-on-2-aminopyridine-modified-triplex-forming-peptide-nucleic-acids
#1
Venubabu Kotikam, Scott D Kennedy, James A MacKay, Eriks Rozners
The development of new RNA-binding ligands is attracting increasing interest in fundamental science and the pharmaceutical industry. The goal of this study was to improve the RNA binding properties of triplex-forming PNAs by further increasing the pKa of 2-aminopyridine (M). Protonation of M was the key for enabling triplex formation at physiological pH in our earlier studies. Substitution on M by an electron donating 4-methoxy substituent resulted in slight destabilization of the PNA-dsRNA triplex, contrary to expected stabilization due to more favorable protonation...
February 11, 2019: Chemistry: a European Journal
https://read.qxmd.com/read/30742632/bibliometric-analysis-of-highly-cited-articles-on-ecosystem-services
#2
Xinmin Zhang, Ronald C Estoque, Hualin Xie, Yuji Murayama, Manjula Ranagalage
This paper presents global research trends involving highly cited articles on ecosystem services from 1981 to 2017 based on a bibliometric analysis of such articles from the SCI-E and SSCI databases of the Web of Science. The analysis revealed that there were 132 highly cited articles, most of which were published between 2005 and 2014. Based on author keywords, the term ecosystem services was strongly linked to biodiversity. The top three journals in terms of total number of highly cited articles published were Ecological Economics, PNAS, and Ecological Indicators...
2019: PloS One
https://read.qxmd.com/read/30683074/hyperactivity-restlessness-is-associated-with-increased-functional-connectivity-in-adults-with-adhd-a-dimensional-analysis-of-resting-state-fmri
#3
Peter Sörös, Eliza Hoxhaj, Patricia Borel, Chiharu Sadohara, Bernd Feige, Swantje Matthies, Helge H O Müller, Katharina Bachmann, Marcel Schulze, Alexandra Philipsen
BACKGROUND: Adult attention-deficit/hyperactivity disorder (ADHD) is a serious and frequent psychiatric disorder of multifactorial pathogenesis. Several lines of evidence support the idea that ADHD is, in its core, a disorder of dysfunctional brain connectivity within and between several neurofunctional networks. The primary aim of this study was to investigate associations between the functional connectivity within resting state brain networks and the individual severity of core ADHD symptoms (inattention, hyperactivity, and impulsivity)...
January 25, 2019: BMC Psychiatry
https://read.qxmd.com/read/30682690/novel-amperometric-genosensor-based-on-peptide-nucleic-acid-pna-probes-immobilized-on-carbon-nanotubes-screen-printed-electrodes-for-the-determination-of-trace-levels-of-non-amplified-dna-in-genetically-modified-gm-soy
#4
Simone Fortunati, Andrea Rozzi, Federica Curti, Marco Giannetto, Roberto Corradini, Maria Careri
A novel amperometric genosensor based on PNA probes covalently bound on the surface of Single Walled Carbon Nanotubes - Screen Printed Electrodes (SWCNT-SPEs) was developed and validated in samples of non-amplified genomic DNA extracted from genetically modified (GM)-Soy. The sandwich assay is based on a first recognition of a 20-mer portion of the target DNA by a complementary PNA Capture Probe (CP) and a second hybridization with a PNA Signalling Probe (SP), with a complementary sequence to a different portion of the target DNA...
January 17, 2019: Biosensors & Bioelectronics
https://read.qxmd.com/read/30662989/structure-of-lateral-heterogeneities-in-a-coarse-grained-model-for-multicomponent-membranes
#5
Sebastian Meinhardt, Friederike Schmid
We study the lateral domain structure in a coarse-grained molecular model for multicomponent lipid bilayers by semi-grandcanonical Monte Carlo simulations. The membranes are filled with liquid ordered (lo) domains surrounded by a liquid disordered (ld) matrix. Depending on the membrane composition and temperature, we identify different morphological regimes: one regime (I) where the lo domains are small and relatively compact, and two regimes (II, II') where they are larger and often interconnected. In the latter two regimes, the ld matrix forms a network of disordered trenches separating the lo domains, with a relatively high content of interdigitated line defects...
January 21, 2019: Soft Matter
https://read.qxmd.com/read/30657037/advances-in-nanoparticle-based-delivery-of-next-generation-peptide-nucleic-acids
#6
Shipra Malik, Brenda Asmara, Zoe Moscato, Jatinder Kaur Mukker, Raman Bahal
BACKGROUND: Peptide nucleic acids (PNAs) belong to the next generation of synthetic nucleic acid analogues. Their high binding affinity and specificity towards the target DNA or RNA makes them the reagent of choice for gene therapy-based applications. OBJECTIVE: To review important gene therapy based applications of regular and chemically modified peptide nucleic acids in combination with nanotechnology Method: Selective research of the literature. RESULTS: However, poor intracellular delivery of PNAs have been a significant challenge...
January 17, 2019: Current Pharmaceutical Design
https://read.qxmd.com/read/30620563/pna-functionalized-adenoviral-vectors-targeting-g-quadruplexes-in-the-p1-promoter-of-bcl-2-proto-oncogene-a-new-tool-for-gene-modulation-in-anti-cancer-therapy
#7
Andrea Patrizia Falanga, Vincenzo Cerullo, Maria Marzano, Sara Feola, Giorgia Oliviero, Gennaro Piccialli, Nicola Borbone
The B-cell lymphoma 2 (Bcl-2) gene encodes for an antiapoptotic protein associated with the onset of many human tumors. Several oligonucleotides (ONs) and ON analogues are under study as potential tools to counteract the Bcl-2 expression. Among these are Peptide Nucleic Acids (PNAs). The absence of charges on PNA backbones allows the formation of PNA/DNA complexes provided with higher stability than the corresponding natural DNA/DNA counterparts. To date, the use of PNAs in antigene or antisense strategies is strongly limited by their inability to cross efficiently the cellular membranes...
January 8, 2019: Bioconjugate Chemistry
https://read.qxmd.com/read/30618153/peptide-nucleic-acid-and-amino-acid-modified-peptide-nucleic-acid-analysis-by-capillary-zone-electrophoresis
#8
Xiaoqian Wang, Linsen Li, Rizwan Ullah Khan, Feng Qu
A rapid, high resolution and low sample consumption CZE method is developed for peptide nucleic acid (PNA) analysis for the first time. 30% (v/v) acetonitrile in PNA sample and 20% (v/v) acetonitrile in 50 mM borax-boric acid (pH 8.7) as BGE were employed after optimization. The calibration curves were linear for PNA concentration ranging from 1.0 to 50.0 μmol/L. LOD and LOQ of PNA were 0.2 μmol/L and 1.0 μmol/L, respectively. Since the commercially available reagent gives rise to huge PNA peak and an apparent impurity peak, the purity of PNA was evaluated to be about 81...
January 7, 2019: Electrophoresis
https://read.qxmd.com/read/30607328/potential-of-peptide-nucleic-acids-in-future-therapeutic-applications
#9
REVIEW
Soheila Montazersaheb, Mohammad Saeid Hejazi, Hojjatollah Nozad Charoudeh
Peptide nucleic acids (PNA) are synthetic analog of DNA with a repeating N-(2-aminoethyl)-glycine peptide backbone connected to purine and pyrimidine nucleobases via a linker. Considering the unique properties of PNA, including resistance to enzymatic digestion, higher biostability combined with great hybridization affinity toward DNA and RNA, it has attracted great attention toward PNA- based technology as a promising approach for gene alteration. However, an important challenge in utilizing PNA is poor intracellular uptake...
November 2018: Advanced Pharmaceutical Bulletin
https://read.qxmd.com/read/30587551/pnas-plus-significance-statements
#10
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
December 26, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/30571359/neonatal-heart-regeneration
#11
Nicholas T Lam, Hesham A Sadek
BACKGROUND: The adult mammalian heart is incapable of meaningful functional recovery after injury, and thus promoting heart regeneration is 1 of the most important therapeutic targets in cardiovascular medicine. In contrast to the adult mammalian heart, the neonatal mammalian heart is capable of regeneration after various types of injury. Since the first report in 2011, a number of groups have reported their findings on neonatal heart regeneration. The current review provides a comprehensive analysis of heart regeneration studies in neonatal mammals conducted to date, outlines lessons learned, and poses unanswered questions...
July 24, 2018: Circulation
https://read.qxmd.com/read/30568026/pnas-plus-significance-statements
#12
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
December 18, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/30538211/pnas-plus-significance-statements
#13
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
December 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/30537432/the-pk-a-values-of-the-catalytic-residues-in-the-retaining-glycoside-hydrolase-t26h-mutant-of-t4-lysozyme
#14
Jacob A Brockerman, Mark Okon, Stephen G Withers, Lawrence P McIntosh
T4 phage lysozyme (T4L) is an enzyme that cleaves bacterial cell wall peptidoglycan. Remarkably, the single substitution of the active site Thr26 to a His (T26H) converts T4L from an inverting to a retaining glycoside hydrolase with transglycosylase activity. It has been proposed that T26H-T4L follows a double displacement mechanism with His26 serving as a nucleophile to form a covalent glycosyl-enzyme intermediate (Kuroki et al. (1999) PNAS 96: 8949-8954). To gain further insights into this or alternative mechanisms, we used NMR spectroscopy to measure the acid dissociation constants (pKa values) and/or define the ionization states of the Asp, Glu, His, and Arg residues in the T4L mutant...
December 7, 2018: Protein Science: a Publication of the Protein Society
https://read.qxmd.com/read/30533422/retroviral-integration-site-selection-a-running-gag
#15
COMMENT
Paul Lesbats, Vincent Parissi
The ability of retroviruses to integrate their genomes into host chromatin is a key step for the completion of their replication cycle. Selection of a suitable chromosomal integration site has been described as a hierarchical mechanism involving both cellular and viral proteins but the exact molecular determinants are still unclear. We recently showed that the spumaretrovirus prototype foamy virus (PFV) Gag protein is acting as a chromatin tether by interacting with the nucleosome acidic patch (Lesbats et al...
November 19, 2018: Microbial Cell
https://read.qxmd.com/read/30514752/pnas-plus-significance-statements
#16
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
December 4, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/30481769/platelet-neutrophil-interactions-are-lower-in-cord-blood-of-premature-newborns
#17
Ijeoma Esiaba, Danilyn M Angeles, Terry-Ann M Milford, Lorena M Salto, Kimberly J Payne, Melissa Y Kidder, Danilo S Boskovic
OBJECTIVE: To quantify platelet-neutrophil interaction by flow cytometry, in newborn cord blood, as a function of gestational age. RATIONALE: Little is known about platelet function markers in the newborn, and developmental variations in these markers are not well described. METHODS: Cord blood samples were obtained from 64 newborns between 23 and 40 weeks' gestation. The neonates were grouped into three categories: preterm (< 34 weeks' gestation, n = 21), late preterm (34 to < 37 weeks' gestation, n = 22), and term (≥37 weeks' gestation, n = 21)...
November 27, 2018: Neonatology
https://read.qxmd.com/read/30471216/circulating-platelet-neutrophil-aggregates-as-risk-factor-for-deep-venous-thrombosis
#18
Jingyi Zhou, Erwen Xu, Kang Shao, Wenyan Shen, Yi Gu, Min Li, Wei Shen
Background Platelet-neutrophil aggregates (PNAs) are fundamental mechanisms linking hemostasis and inflammatory processes. Elevated level of PNAs have been reported in inflammatory diseases and coronary artery diseases. However, studies on the correlation between PNAs formation and deep venous thrombosis (DVT) are not available. Methods A total of 92 participants were involved in this study, including 32 cases with DVT and 60 cases without DVT. Blood samples coagulated by K2-EDTA or sodium citrate were prepared for blood cell count and blood smears...
November 24, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://read.qxmd.com/read/30465538/-gsa-genome-sequence-archive
#19
Si Si Zhang, Ting Ting Chen, Jun Wei Zhu, Qing Zhou, Xu Chen, Yan Qing Wang, Wen Ming Zhao
The Genome Sequence Archive (GSA), a new data repository for raw sequence reads in China, has been developed in compliance with the International Nucleotide Sequence Database Collaboration (INSDC) standards. It supports data generated from a variety of sequencing platforms ranging from Sanger sequencing to single-cell sequencing and provides data storing and sharing services freely for worldwide scientific communities. Since it went online in late 2015, GSA has archived more than 500 TB data and been acknowledged by many high-profile journals, including Cell, Nature, PNAS, GPB, etc...
November 20, 2018: Yi Chuan, Hereditas
https://read.qxmd.com/read/30386633/evaluation-of-aflibercept-and-ziv-aflibercept-binding-affinity-to-vascular-endothelial-growth-factor-stability-and-sterility-after-compounding
#20
Julia de Lima Farah, Ronaldo Sano, Ieda Maria Longo Maugéri, Daniela Teixeira, Mayari Eika Ishimura, Gabriela Martins, Lycia M J Mimica, Cely Barreto da Silva, Carsten H Meyer, João Rafael de Oliveira Dias, Gabriel Costa de Andrade, Michel Eid Farah
Purpose: To investigate the binding affinity, stability, and sterility of aflibercept and ziv-aflibercept to vascular endothelial growth factor (Holash et al. in Proc Natl Acad Sci USA 99(17):11393-11398, 2002. 10.1073/pnas.172398299) after compounding and storage for up to 28 days at 4 °C and - 8 °C. Methods: Tuberculin-type 1-mL syringes were prepared containing aflibercept (40 mg/mL) and ziv-aflibercept (25 mg/mL). Samples were stored at 4 °C and - 8 °C for 0, 14, and 28 days and evaluated for the binding affinity of anti-VEGF to VEGF and stability using enzyme-linked immunosorbent assays...
2018: International Journal of Retina and Vitreous
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