Michael A Turner, Kristin E Cox, Shanglei Liu, Nicholas Neel, Siamak Amirfakhri, Hiroto Nishino, Mojgan Hosseini, Joshua A Alcantara, Amer Ali Abd El-Hafeez, Thinzar M Lwin, Kavita Mallya, Joseph R Pisegna, Satish K Singh, Pradipta Ghosh, Robert M Hoffman, Surinder K Batra, Michael Bouvet
Poor visualization of polyps can limit colorectal cancer screening. Fluorescent antibodies to mucin5AC (MUC5AC), a glycoprotein upregulated in adenomas and colorectal cancer, could improve screening colonoscopy polyp detection rate. Adenomatous polyposis coli flox mice with a Cdx2-Cre transgene ( CPC-APC ) develop colonic polyps that contain both dysplastic and malignant tissue. Mice received MUC5AC-IR800 or IRdye800 as a control IV and were sacrificed after 48 h for near-infrared imaging of their colons. A polyp-to-background ratio (PBR) was calculated for each polyp by dividing the mean fluorescence intensity of the polyp by the mean fluorescence intensity of the background tissue...
April 11, 2023: Current Issues in Molecular Biology