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Thaísa T Pinheiro, Lázaro E P Peres, Eduardo Purgatto, Rodrigo R Latado, Rodolfo A Maniero, Mônica M Martins, Antonio Figueira
Complementation of the "Micro-Tom" tomato tangerine mutant with a Citrus CRTISO allele restores the wild-type fruit carotenoid profile, indicating that the Citrus allele encodes an authentic functional carotenoid isomerase. Citrus fruits are rich in carotenoids; the genus offers a large diversity in composition, yet to be fully explored to improve fruit nutritional quality. As perennial tree species, Citrus lack the resources for functional genetic studies, requiring the use of model plant systems...
February 8, 2019: Plant Cell Reports
Chuying Chen, Zhengpeng Nie, Chunpeng Wan, Jinyin Chen
Xinyu tangerine is a citrus fruit that has enjoyed great popularity in China for its fewer dregs and abundant nutrients. However, it is considered an easily perishable fruit that is vulnerable to various pathogenic fungal infections, especially by Penicillium italicum , which reduces its storage life and commercial value. Normally, to reduce the losses caused by fungal deterioration of harvested fruit, polysaccharide-based edible coating, containing natural antimicrobial agents (e.g. plant extracts), have been applied...
January 28, 2019: Biomolecules
Yunpeng Gai, Bing Liu, Haijie Ma, Lei Li, Xinglong Chen, Susan Moenga, Brendan Riely, Amna Fayyaz, Mingshuang Wang, Hongye Li
The tangerine pathotype of A. alternata, which produces a unique host-selective ACT toxin causes brown spots on citrus leaves and fruits. In this study, we report a methionine biosynthesis regulator (MetR), which belong to bZIP transcription factor, is required for methionine metabolism, oxidative stress tolerance and pathogenicity. We generated two ΔAaMetR mutants in the tangerine pathotype of Alternaria alternata and investigated the resulting mutant phenotypes. The ΔAaMetR disruption mutant grew poorly in the absence of methionine and unable to produce conidia...
February 2019: Microbiological Research
Tao Li, Runtao Tian, Xinlan Yu, Lei Sun, Yi He, Peishan Xie, Shuangcheng Ma
Background: The use of HPTLC fingerprinting for the analysis of traditional Chinese medicines (TCMs) usually involves several image-processing steps. However, these image-processing steps are time consuming Objective: We describe a new approach that applies artificial neural networks (ANN) directly to raw high-performance thin-layer chromatography HPTLC images. Methods: This approach combines image processing and chemometric modeling and was used to classify TCMs [dried tangerine eel (Chen Pi), green tangerine peel (Qing Pi), immature bitter orange fruit, and bitter orange fruit (Zhi Qiao)]...
December 3, 2018: Journal of AOAC International
Lixin Wang, Ying Cao, Ye Tian, Guangheng Luo, Xiushu Yang, Zhaolin Sun
OBJECTIVES: The present study aimed to investigate the influence of urine on re-epithelialization in canine prostatic urethra after prostatectomy and explore possible causes. METHOD: We established two groups of prostatic canine models. The first group contained urine that canines underwent the surgery by two-micron laser resection of the prostate-tangerine technique (TmLRP-TT), and no transurethral catheter was required. The second group was without urine that canines accepted the surgery by TmLRP-TT add ureter skin ostomy urine bypass...
November 7, 2018: International Urology and Nephrology
Guangning Kou, Zhenqing Li, Chao Wu, Yang Liu, Yan Hu, Liya Guo, Xiaoyu Xu, Zhiqin Zhou
Mitochondrial biogenesis is a key factor, which influences the function of skeletal muscle. Increasingly, flavonoids are reported to have the potential ability of regulating mitochondrial biogenesis. In this study, we investigated the effects of tangeretin, a polymethoxylated flavonoid isolated from mandarin fruits, on mitochondrial biogenesis and its underlying mechanisms. The tangeretin was obtained from the peel of "Dahongpao" tangerine by macroporous adsorptive resins combined with preparative-high performance liquid chromatography...
November 14, 2018: Journal of Agricultural and Food Chemistry
Zhenqing Li, Ziyan Zhao, Zhiqin Zhou
In this study, a preparative separation method was established to simultaneously isolate the polymethoxylated flavones (PMFs) from the peel of "Dahongpao" tangerine using macroporous adsorptive resins (MARs) combined with prep-HPLC. The total PMFs were enriched using MARs to remove most sugars, water-soluble pigments, and flavanones, and the eluents obtained were analyzed by ultra-performance liquid chromatography (UPLC) to determine the PMF composition. The separation and purification of PMFs were carried out by using a mass spectrometry-guided prep-HPLC with a gradient elution of acetonitrile-water ( v / v ), simultaneously...
October 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Rajappa Rashmi, Salai Bojan Magesh, Kunka Mohanram Ramkumar, Sarvajayakesavulu Suryanarayanan, Madhunapantula Venkata SubbaRao
Background: Naringenin is a bioactive flavonoid found in grapes and citrus fruits including tangelo, blood orange, lemons, and tangerines. The aims of this study were to investigate the ability of naringenin to scavenge free radicals and determine its ability to protect animals from streptozotocin (STZ) -induced liver damage. Methods: The free radical-scavenging activity of naringenin was evaluated by in vitro cell-free assay systems. In animals, the antioxidant potential of orally administered 50 and 100 mg/kg body weight naringenin for 45 days was assessed by measuring TBARS, lipid hydroperoxides, SOD, catalase, GST, GPx, and glutathione levels in liver homogenates prepared from animals injected intraperitoneally with multiple low dose streptozotocin at 50 mg/kg for five consecutive days...
October 2018: Reports of Biochemistry & Molecular Biology
Augusto Ramírez-Godoy, María Del Pilar Vera-Hoyos, Natalia Jiménez-Beltrán, Hermann Restrepo-Diaz
Efficient, economic, and simple monitoring methods are important to develop successful integrated management for Asian citrus psyllid (ACP) especially in developing countries. Two 5-wk tests were carried out to study the capture of ACP adults using yellow sticky traps by adding commercial citrus fruit scents (lemon, tangerine, or orange) or commercial coconut oil (first test) and to compare these baits with commercial lures (Alpha Scents and Pest Wizard) (second test) under natural infestation conditions. In the first test, the following treatments were carried out: yellow sticky traps baited with 1) coconut oil; 2) lemon, tangerine, or orange commercial scents; or 3) unbaited yellow sticky tramps (control)...
October 3, 2018: Journal of Economic Entomology
Emily K Heying, Kaitlin Leary Ziemer, Jacob P Tanumihardjo, Natalia Palacios-Rojas, Sherry A Tanumihardjo
Background: Consumption of provitamin A carotenoid biofortified crops, such as maize, supports vitamin A (VA) status in animals and humans. Laying hens that consume β-cryptoxanthin-biofortified maize deposit β-cryptoxanthin into egg yolk. Objective: We investigated whether β-cryptoxanthin-biofortified egg consumption would affect VA status of male Mongolian gerbils (Meriones unguiculatus) compared with white-yolked eggs. Methods: β-Cryptoxanthin-biofortified egg yolk, produced in hens fed biofortified orange maize or tangerine-fortified maize feeds, was freeze-dried and fed to gerbils...
August 1, 2018: Journal of Nutrition
Ayokunle O Ademosun, Adeniyi A Adebayo, Ganiyu Oboh
Background Citrus peels have been reported useful in folk medicine for the management of cardiovascular diseases, but there is dearth of information on the possible mechanisms for their therapeutic action. The aim of this study was to investigate the effect of methanolic extracts from some citrus [lime (Citrus limon), tangerine (Citrus reticulata), shaddock (Citrus maxima)] peels on some enzymes relevant to the management of cardiovascular diseases [monoamine oxidase (MAO), phosphodiesterase-5 (PDE-5) and angiotensin-1-converting enzyme (ACE)]...
August 11, 2018: Journal of Complementary & Integrative Medicine
Guliang Yang, Shiming Li, Yiwen Yang, Li Yuan, Peilei Wang, Hui Zhao, Chi-Tang Ho, Chi-Chen Lin
Psoriasis is a chronic and benign proliferative skin disease. Flavonoids in chenpi (aged tangerine peel) from tangerine ( Citrus reticulate Blanco), such as nobiletin (Nob), tangeretin, and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (5-HPMF), possess anti-inflammation and regulation of immune activity among others. In this study, psoriasis-like skin lesions were induced by 12- O-tetradecanoylphorbol-13-acetate (TPA), and the preventive effect of Nob and 5-HPMF on psoriasis-like skin lesions was evaluated. Results showed that skin lesions were dramatically reduced by Nob and 5-HPMF...
August 8, 2018: Journal of Agricultural and Food Chemistry
Antoni Delpino-Rius, Alexis Marsol-Vall, Jordi Eras, Montse Llovera, Miguel Ángel Cubero, Mercè Balcells, Ramon Canela-Garayoa
Here we analysed the content of primary and secondary metabolites in nine types of industrially processed fibres derived from the juice industry. Specifically, we examined fibre from: apple, peach, and pear, as non-citrus fruits; the peel and flesh of orange and tangerine, and lemon flesh, as citrus fruits; and carrot, as vegetable. Regarding primary metabolites, the sugar content ranged from 21.6 mg/g in lemon to 290 mg/g in orange peel and lower mass organic acid content ranged from 25.0 mg/g in pear to 250 mg/g in lemon...
September 2018: Food Research International
Muhammad Arshad, Muhammad Irfan Ullah, Jawwad A Qureshi, Muhammad Afzal
Feeding damage by arthropods exposes plants to pathogens and interferes with plant physiological processes. Feeding by the citrus leafminer (CLM), Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), larva exposes leaf cuticle to the causal pathogens of the citrus canker disease. We investigated the impact of larval feeding on photosynthetic rate (Pn), CO2 exchange rate (Ci), and H2O exchange rate (Wi) in eight citrus cultivars [Citrus mandarins (Kinnow, Seedless Kinnow, Feutrell's early), Citrus sinensis (Succari, Salustiana), Citrus tangerines (Fairchild), Citrus limon (China lemon), and Citrus paradisi Macfad (Grapefruit)] under natural conditions in comparison with control plants protected from larval feeding...
September 26, 2018: Journal of Economic Entomology
Mingshuang Wang, Xiao Yang, Ruoxin Ruan, Huilan Fu, Hongye Li
The COP9 signalosome (CSN) is a highly conserved protein complex involved in the ubiquitin-proteasome system. Its metalloisopeptidase activity resides in subunit 5 (CSN5). Functions of csn5 in phytopathogenic fungi are poorly understood. Here, we knocked out the csn5 ortholog ( Aacsn5 ) in the tangerine pathotype of Alternaria alternata . The Δ Aacsn5 mutant showed a moderately reduced growth rate compared to the wildtype strain and was unable to produce conidia. The growth of Δ Aacsn5 mutant was not affected in response to oxidative and osmotic stresses...
2018: Frontiers in Microbiology
Xiaowen Dou, Lei Zhang, Congmin Liu, Qian Li, Jiaoyang Luo, Meihua Yang
A new kind of labelled antibody was synthesized by modifying gold nanoparticles (AuNPs) with the fluorophore rhodamine B isothiocyanate (RBITC) and a secondary antibody (IgG). The conjugate thus obtained was used in a competitive sandwich assay with a turn-on signal change. It was designed to detect the organophosphorus pesticide chlorpyrifos. The fluorescence of the RBITC-labeled gold immunoprobe with emission at 575 nm and excitation at 556 nm is almost completely quenched. If, however, cysteamine is added, the fluorophore is released from the labeled secondary antibody and fluorescence increases in accordance with the quantity of secondary antibody bound to the sandwich...
December 8, 2017: Mikrochimica Acta
Francesca Garganese, Antonio Ippolito, Valentina di Rienzo, Concetta Lotti, Cinzia Montemurro, Simona M Sanzani
BACKGROUND: Alternaria brown spot is one of the most important diseases of tangerines and their hybrids worldwide. To set up effective control strategy, the accurate detection and identification of the species responsible for the diseases is crucial. However, characterization based on morphology and/or multilocus genetic approaches is time consuming, requires great expertise and sometimes is not conclusive. Therefore, the set-up of a rapid and efficient DNA-based assay might be of paramount importance...
September 2018: Journal of the Science of Food and Agriculture
Jinlong Wu, Wenjun Deng, Dingbo Lin, Xiuxin Deng, Zhaocheng Ma
The inherent allergens of citrus fruits, such as Cit s 1, Cit s 2, Cit s 3 can cause allergic reactions. A better understanding of the genetic factors (cultivar to cultivar) affecting the allergenic potential of citrus fruits would be beneficial for further identification of hypoallergenic genotypes. In the present study, an immunoblotting quantification approach was adopted to assess the potential allergenicity of 21 citrus cultivars, including nine subgroups (tangerine, satsuma, orange, pummelo, grapefruit, lemon, kumquat, tangor, and tangelo)...
February 28, 2018: Journal of Agricultural and Food Chemistry
Javed Nawab, Saad Farooqi, Wang Xiaoping, Sardar Khan, Asad Khan
Food safety is a major concern worldwide and human beings are frequently exposed to potentially toxic metals (PTMs) through consumption of vegetables, fruits, and cereal crops grown in contaminated areas. The present study investigates the concentrations of PTMs such as chromium (Cr), nickel (Ni), zinc (Zn), arsenic (As), cadmium (Cd), and lead (Pb) in the foodstuffs (fruits, vegetables, and cereals) collected from different markets of Khyber Pakhtunkhwa, Pakistan. Samples of fruits (banana, tangerine, apple, and guava), vegetables (tomato, onion, potato, pea, and lady finger), and cereals (rice, kidney beans, and chick peas) were acid-extracted and analyzed using ICP-MS...
February 2018: Environmental Science and Pollution Research International
Yolanda Picó, Mohamed A El-Sheikh, Ahmed H Alfarhan, Damià Barceló
The occurrence of pesticide residues in fruits was determined by a target method for 62 analytes and a wide scope screening method against a database of 500 pesticides. Limits of quantification (LOQs) were from 0.5 to 6.3 μg kg-1 for the target method and from 0.5 to 119 μg kg-1 for the non-target. Thirty samples: dates, apples, oranges, tangerines, lemons and grapefruits were selected because their high consumption, except lemons and grapefruits that were to cover all citrus fruits. Using the target method, 15 compounds (mostly insecticides and fungicides) out of 62 pesticides (organophosphorus, carbamates, pyrethroids, chloroacetanilides, triazines, triazoles, imidazoles, etc...
January 2018: Food and Chemical Toxicology
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