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Grzegorz Ślifirski, Marek Król, Jerzy Kleps, Szymon Ulenberg, Mariusz Belka, Tomasz Bączek, Agata Siwek, Katarzyna Stachowicz, Bernadeta Szewczyk, Gabriel Nowak, Andrzej Bojarski, Anna E Kozioł, Jadwiga Turło, Franciszek Herold
The study enabled obtaining a number of new derivatives of 4-aryl-pyrido[1,2-c]pyrimidine 9.1-9.27 having conformationally restricted tryptamine moiety. In vitro studies (RBA) have shown that derivatives 9.1, 9.2, 9.4, 9.7, 9.9, 9.14 and 9.27 exhibit high affinity to molecular targets 5-HT1A receptor and SERT protein. In general, compounds with an unsubstituted or a para-substituted benzene ring of the pyrido[1,2-c]pyrimidine residue in the terminal part were characterized by higher binding ability, which can be justified by the greater flexibility of the structure...
January 22, 2019: European Journal of Medicinal Chemistry
Tugba NurcanYuksel, Muhammed Yayla, Zekai Halici, Elif Cadirci, Beyzagul Polat, Duygu Kose
Serotonin exerts anti-inflammatory, antioxidant and antiapoptotic effects through 5-HT7 receptors. The present study determined the role of 5-HT7 receptors in glutamate-induced neurotoxicity by using human SH-SY5Y neuroblastoma cells. The cells were pretreated with different concentrations of 5-HT7 receptor agonist LP44 and antagonist SB269970 for 60 min, followed by treatment with glutamate. Cell proliferation was measured using xCELLigence system. Treatment with all the concentrations of LP44 significantly protected the cells from the toxic effects of glutamate after 24, 48 and 72 h...
January 24, 2019: Neurotoxicology and Teratology
Minako Ito, Kyoko Komai, Setsuko Mise-Omata, Mana Iizuka-Koga, Yoshiko Noguchi, Taisuke Kondo, Ryota Sakai, Kazuhiko Matsuo, Takashi Nakayama, Osamu Yoshie, Hiroko Nakatsukasa, Shunsuke Chikuma, Takashi Shichita, Akihiko Yoshimura
In addition to maintaining immune tolerance, FOXP3+ regulatory T (Treg ) cells perform specialized functions in tissue homeostasis and remodelling1,2 . However, the characteristics and functions of brain Treg cells are not well understood because there is a low number of Treg cells in the brain under normal conditions. Here we show that there is massive accumulation of Treg cells in the mouse brain after ischaemic stroke, and this potentiates neurological recovery during the chronic phase of ischaemic brain injury...
January 2, 2019: Nature
Gniewomir Latacz, Adam S Hogendorf, Agata Hogendorf, Annamaria Lubelska, Joanna M Wierońska, Monika Woźniak, Paulina Cieślik, Katarzyna Kieć-Kononowicz, Jadwiga Handzlik, Andrzej J Bojarski
Close structural analogues of 5-carboxamidotryptamine (5-CT) based on the newly discovered indole-imidazole scaffold were synthesized and evaluated to search for a 5-HT7 receptor agonist of higher selectivity. In vitro drug-likeness studies and in vivo pharmacological evaluation of potent and selective low-basicity 5-HT7 receptor agonists, previously published 7 (3-(1-ethyl-1 H -imidazol-5-yl)-1 H -indole-5-carboxamide, AH-494) and 13 (3-(1-methyl-1 H -imidazol-5-yl)-1 H -indole-5-carboxamide), have supported their usefulness as pharmacological tools...
November 1, 2018: MedChemComm
Olivia M Balcer, Matthew Seager, Scott D Gleason, Xia Li, Kurt Rasmussen, Julia K Maxwell, George Nomikos, Aldemar Degroot, Jeffrey M Witkin
The 5-HT7 receptor is the most recently identified receptor subtype within a family of 5-HT receptors activated by the neurotransmitter serotonin. There has been significant interest in investigating the potential role of this receptor in psychiatric disorders including depression, anxiety, and schizophrenia. Behaviors of 5-HT7 +/+ (wild-type or WT) and 5-HT7 -/- (receptor knockout or KO) mice were compared across 10 different assays (7 for anxiety, 1 for depression, 2 for psychosis) to identify differences that could indicate clinical potential for 5-HT7 receptor antagonism...
December 10, 2018: Behavioural Brain Research
Dorota Łażewska, Rafał Kurczab, Małgorzata Więcek, Grzegorz Satała, Katarzyna Kieć-Kononowicz, Jadwiga Handzlik
A series of 2-amino-4-(4-methylpiperazin-1-yl)-1,3,5-triazines was designed based on previously published 2-amino-4-benzyl-(4-methylpiperazin-1-yl)-1,3,5-triazines in order to evaluate the role of a linker between the triazine moiety and an aromatic substituent for the human serotonin 5-HT6 receptor affinity. As new linkers two carbon atoms (ethyl or ethenyl) or an oxyalkyl chain (methoxy, 2-ethoxy, 2-propoxy) were introduced. Affinities of the compounds for the 5-HT6 R as the main target, and for the 5-HT1A R, 5-HT7 R and D2 R as competitive ones, were determined in the radioligand binding assays...
November 26, 2018: Bioorganic Chemistry
Andrea Hembre Ulsund, Marie Dahl, Thomas M Frimurer, Ornella Manfra, Thue W Schwartz, Finn Olav Levy, Kjetil Wessel Andressen
According to early models of GPCR signaling, G proteins only interact with activated receptors. However, some GPCRs were shown to assemble with G proteins before receptor activation, in accordance with more recent models. Previously, we found that the 5-HT7 receptor, as opposed to the 5-HT4 receptor, was preassociated with Gs , but the molecular determinants for this interaction are still elusive. In a series of chimeric 5-HT7 receptors with intracellular segments from 5-HT4 , we determined the receptor-G protein interaction by performing antibody-immobilized fluorescence recovery after photobleaching and fluorescence resonance energy transfer...
December 3, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Julia Maxwell, Scott D Gleason, Julie Falcone, Kjell Svensson, Olivia M Balcer, Xia Li, Jeffrey M Witkin
5-HT7 receptors have been suggested to play a role in the regulation of psychiatric disorders. The experimental literature however is not fully consistent on this possibility. Two selective 5-HT7 receptor antagonists, DR-4004 and SB-269970, were evaluated in mouse models used to detect drugs used to treat anxiety, depression, or schizophrenia. A 5-HT-induced hypothermia assay was used to define the doses of DR-4004 and SB-269970 predicted to impact 5-HT7 receptors in the brain in vivo. 5-HT induced hypothermia in wildtype mice by either i...
November 21, 2018: Behavioural Brain Research
Cristina López, Miriam Gómez-Roso, José Ángel García-Pedraza, María Luisa Martín, Asunción Morán, Mónica García-Domingo
Although depression and cardiovascular diseases are related, the role of antidepressants, as fluoxetine (increasing serotonin levels), within cardiac regulation remains unclear. We aimed to determine whether fluoxetine modifies the pharmacological profile of serotonergic influence on vagal cardiac outflow. Rats were treated with fluoxetine (10 mg/; p.o.) for 14 days or equivalent volumes of drinking water (control group); then, they were pithed and prepared for vagal stimulation. Bradycardic responses were obtained by electrical stimulation of the vagal fibers (3, 6, and 9 Hz) or i...
November 21, 2018: Canadian Journal of Physiology and Pharmacology
Stevie Margaret Lockwood, Kirsty Bannister, Anthony H Dickenson
Osteoarthritis (OA) is a debilitating conditioning with pain as the major clinical symptom. Understanding the mechanisms that drive OA-associated chronic pain is crucial for developing the most effective analgesics. Although the degradation of the joint is the initial trigger for the development of chronic pain, the discordance between radiographic joint damage and the reported pain experience in patients, coupled with clinical features that cannot be explained by purely peripheral mechanisms, suggest there are often other factors at play...
November 21, 2018: Journal of Neurophysiology
Xenia Gonda, Samata R Sharma, Frank I Tarazi
Vortioxetine is a novel antidepressant drug approved for the treatment of major depressive disorder (MDD) in adults. It is formulated into tablets and has a dose range of 5-20 mg. The recommended starting dose is 10 mg administered orally once daily without the need for food. Areas covered: This review focuses on the preclinical and clinical discovery of vortioxetine. It analyzes the pharmacological, neurochemical, and behavioral mechanisms of the medication and how these contribute to its potential therapeutic advantages as described in published preclinical and clinical studies and product labels...
January 2019: Expert Opinion on Drug Discovery
Diogo A Fonseca, Cristina Teixeira, Félix Carvalho, Eduarda Fernandes, Pedro E Antunes, Manuel J Antunes, Maria Dulce Cotrim
3,4-Methylenedioxymethamphetamine (MDMA or "ecstasy") is a popular recreational drug of abuse. In addition to its characteristic psychotropic effects, important cardiovascular effects have been described such as increased blood pressure and heart rate. MDMA was previously shown to behave as a partial agonist on 5-hydroxytryptamine (5-HT) receptors in the human internal thoracic artery in vitro, involving the 5-HT2A subtype. Here, we studied the interaction of MDMA (400, 800 and 1600 μM) with the following 5-HT receptor agonists: 5-carboxamidotryptamine (5-CT, full agonist for the 5-HT1 , 5-HT2 , 5-HT5 , 5-HT6 and 5-HT7 receptors) and sumatriptan (selective 5-HT1B/1D receptors agonist)...
November 15, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Karolina Pytka, Monika Głuch-Lutwin, Elżbieta Żmudzka, Kinga Sałaciak, Agata Siwek, Katarzyna Niemczyk, Maria Walczak, Magdalena Smolik, Adrian Olczyk, Adam Gałuszka, Jarosław Śmieja, Barbara Filipek, Jacek Sapa, Marcin Kołaczkowski, Katarzyna Pańczyk, Anna Waszkielewicz, Henryk Marona
Numerous studies have proven that both stimulation and blockade of 5-HT1A and the blockade of 5-HT7 receptors might cause the anxiolytic-like effects. Biased agonists selectively activate specific signaling pathways. Therefore, they might offer novel treatment strategies. In this study, we investigated the anxiolytic-like activity, as well as the possible mechanism of action of 1-[(2,5-dimethylphenoxy)propyl]-4-(2-methoxyphenyl)piperazine hydrochloride (HBK-17). In our previous experiments, HBK-17 showed high affinity for 5-HT1A and 5-HT7 receptors and antidepressant-like properties...
2018: Frontiers in Pharmacology
Justyna Kalinowska-Tłuścik, Agata Piaskowska, Marcin Kołaczkowski
Nowadays, a search for antagonists co-acting on serotonin receptor subtypes 6 and 7 (5-HT6 R and 5-HT7 R, respectively) is of great interest due to the increasing number of patients suffering from dementia and related behavioural and psychological symptoms. The X-ray crystal structures of four promising multifunctional ligands in the hydrochloride forms were determined, namely 4-(6-fluoro-1,2-benzoxazol-3-yl)-1-[3-(3-methylbenzenesulfonamido)propyl]piperidin-1-ium chloride, C22 H27 FN3 O3 S+ ·Cl- , (I), 4-(6-fluoro-1,2-benzoxazol-3-yl)-1-[4-(5-fluoro-3-methylbenzo[b]thiophene-2-sulfonamido)butyl]piperidin-1-ium chloride, C25 H28 F2 N3 O3 S2 + ·Cl- , (II), 4-(6-fluoro-1,2-benzoxazol-3-yl)-1-[4-(6-fluorobenzo[b]thiophene-2-sulfonamido)butyl]piperidin-1-ium chloride, C24 H26 ClFN3 O3 S2 + ·Cl- , (III), and 4-(6-fluoro-1,2-benzoxazol-3-yl)-1-[3-(3-chloro-4-fluorobenzenesulfonamido)propyl]piperidin-1-ium chloride, C21 H22 ClF2 N3 O3 S2 + ·Cl- , (IV)...
November 1, 2018: Acta Crystallographica. Section C, Structural Chemistry
Raphael R Perim, Daryl P Fields, Gordon S Mitchell
KEY POINTS SUMMARY: Concurrent 5-HT2A (Q pathway) and 5-HT7 (S pathway) serotonin receptor activation cancels phrenic motor facilitation due to mutual cross-talk inhibition Spinal PKCδ or PKA inhibition restores phrenic motor facilitation with concurrent Q and S pathway activation, demonstrating a key role for these kinases in cross-talk inhibition Spinal PKCδ inhibition enhances adenosine-dependent severe AIH-induced pLTF (S pathway), consistent with relief of cross-talk inhibition...
November 1, 2018: Journal of Physiology
Lara Costa, Lara Maria Sardone, Carmela Maria Bonaccorso, Simona D'Antoni, Michela Spatuzza, Walter Gulisano, Maria Rosaria Tropea, Daniela Puzzo, Marcello Leopoldo, Enza Lacivita, Maria Vincenza Catania, Lucia Ciranna
We have previously demonstrated that activation of serotonin 5-HT7 receptors (5-HT7 R) reverses metabotropic glutamate receptor-mediated long term depression (mGluR-LTD) in the hippocampus of wild-type (WT) and Fmr1 Knockout (KO) mice, a model of Fragile X Syndrome (FXS) in which mGluR-LTD is abnormally enhanced. Here, we have investigated intracellular mechanisms underlying the effect of 5-HT7 R activation using patch clamp on hippocampal slices. Furthermore, we have tested whether in vivo administration of LP-211, a selective 5-HT7 R agonist, can rescue learning and behavior in Fmr1 KO mice...
2018: Frontiers in Molecular Neuroscience
Daniele Vigli, Laura Rusconi, Daniela Valenti, Paolo La Montanara, Livia Cosentino, Enza Lacivita, Marcello Leopoldo, Elena Amendola, Cornelius Gross, Nicoletta Landsberger, Giovanni Laviola, Charlotte Kilstrup-Nielsen, Rosa A Vacca, Bianca De Filippis
Mutations in the X-linked cyclin-dependent kinase-like 5 (CDKL5) gene cause CDKL5 Deficiency Disorder (CDD), a rare neurodevelopmental syndrome characterized by severe behavioural and physiological symptoms. No cure is available for CDD. CDKL5 is a kinase that is abundantly expressed in the brain and plays a critical role in neurodevelopmental processes, such as neuronal morphogenesis and plasticity. This study provides the first characterization of the neurobehavioural phenotype of 1 year old Cdkl5-null mice and demonstrates that stimulation of the serotonin receptor 7 (5-HT7 R) with the agonist molecule LP-211 (0...
October 13, 2018: Neuropharmacology
Anna Partyka, Magdalena Jastrzębska-Więsek, Lucyna Antkiewicz-Michaluk, Jerzy Michaluk, Agnieszka Wąsik, Vittorio Canale, Paweł Zajdel, Marcin Kołaczkowski, Anna Wesołowska
The aim of the present study was to investigate and compare the ability of three novel 5-HT6 and/or 5-HT7 receptor antagonists as follows: PZ-668-a preferential 5-HT6 antagonist; PZ-1433-a preferential 5-HT7 antagonist; and ADN-1184-a monoaminergic ligand with potent 5HT6/7 antagonist properties, to augment the effect of antidepressant drugs with different mechanisms of action (escitalopram, reboxetine, and bupropion) in the forced swim test in rats. In neurochemical ex vivo experiments, the influence of the tested compounds on levels of monoamines and their metabolites were determined in the rat frontal cortex, in addition to behavioral experiments...
October 9, 2018: Behavioural Brain Research
Arvind Kumar, Harpreet Singh, Amrita Mishra, Arun K Mishra
OBJECTIVE: Aripiprazole,a synthetic compound, obtained by chemical modification of the structure of quinolinone is considered as an atypical antipsychotic drug. The present review is an attempt to summarize the updated information related to reported chemistry and pharmacology of Aripiprazole. DEVELOPMENT: Aripiprazole, under development by Otsuka Pharmaceutical, was approved by the U.S. Food and Drug Administration (USFDA) in the end months of 2002 to with an aim to treat patients with schizophrenia...
October 8, 2018: Current Drug Discovery Technologies
Hyung Gon Lee, Ji A Song, Dong Soo Han, Kyeong Wan Woo, Myung Ha Yoon
This study examined the effects of intrathecal areca nut on spinal nerve-ligated and chemotherapy-induced neuropathic pain (NP), and investigated the relevance of spinal 5-hydroxytryptamine (5-HT) and α2-adrenergic receptors to those effects. For drug administration, intrathecal catheters were inserted into the subarachnoid space of male Sprague-Dawley rats. NP was induced either by spinal nerve ligation (left spinal nerves L5 and L6) or by chemotherapeutic injection (intraperitoneal cisplatin, 2 mg/kg/day, once daily for 4 days)...
2018: Pharmacology
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