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Temperature buffer

Sarita Puri, Tapan K Chaudhuri
GroEL is the most commonly used chaperonin protein for both in-vitro refolding of aggregating proteins as well as in-vivo solubilization of over-expressed aggregation-prone proteins of therapeutic and biotechnological applications. But sometimes the stress conditions like heat and a load of over-expressed/unfolded/misfolded proteins lead to a decrease in structural stability and functional efficiency of GroEL, which results in less recovery of substrate protein through the chaperone-mediated refolding process...
February 13, 2019: International Journal of Biological Macromolecules
Arash Razmi, Arezoo Golestanipour, Maryam Nikkhah, Abdolreza Bagheri, Masoud Shamsbakhsh, Saeed Malekzadeh-Shafaroudi
Current techniques for plant virus detection, such as RT- PCR and ELISA, require multistep procedures and rely on sophisticated equipment. Due to the global spread of plant viruses, the development of simpler, faster and cheaper assay methods is inevitable. Gold nanoparticles (AuNPs) had raised much interest during recent years due to their novel optical properties or diagnostic purposes. The localized surface plasmon resonance (LSPR1 ) of AuNPs had been used in the development of novel colorimetric nano-biosensing systems...
February 13, 2019: Journal of Virological Methods
Laura M Doyle, Anant Navanithan Sharma, Ganapathy Gopalrathnam, Lihua Huang, Scott Bradley
In our previous published work, we reported rapid Polysorbate80 (PS80) oxidation in histidine buffer after brief stainless steel exposure and the ability of citrate and EDTA to prevent this oxidation. The focus of our current study was to mechanistically understand PS80 oxidation by studying the impact of temperature, light, stainless steel and the role of citrate and EDTA. Additionally, PS80 oxidation was studied in three different buffer sytems: histidine, citrate, and phosphate. When the PS80 containing buffers were exposed to elevated temperature of 50 °C in glass containers, no PS80 oxidation was observed in either histidine and citrate buffer systems after 30 days; however, PS80 oxidation was observed in phosphate buffer within 14 days...
February 15, 2019: PDA Journal of Pharmaceutical Science and Technology
Yu Han, Wai Kit Ng, Ying Xue, Qiang Li, Kam Sing Wong, Kei May Lau
A compact, efficient, and monolithically grown III-V laser source provides an attractive alternative to bonding off-chip lasers for Si photonics research. Although recent demonstrations of microlasers on (001) Si wafers using thick metamorphic buffers are encouraging, scaling down the laser footprint to nanoscale and operating the nanolasers at telecom wavelengths remain significant challenges. Here, we report a monolithically integrated in-plane InP/InGaAs nanolaser array on (001) silicon-on-insulator (SOI) platforms with emission wavelengths covering the entire C band (1...
February 15, 2019: Optics Letters
Bo Jin, Yanan Wang, Tingting Zhang, Wanting Yin, Dongfeng Zhang, Haihong Huang, Chen Ma
SY0916 has been proven to be a potent treatment agent against rheumatoid arthritis in preclinical studies and has been shown to be safe in phase I clinical trials. However, SY0916 is unstable in water, which is frequently used in pharmaceutical development processes. The degradation behaviour and stability of SY0916 in aqueous solutions were investigated at different pH levels, periods of time, and temperatures. Two degradation products (DPs) were successfully separated and characterized by liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HRMS/MS), liquid chromatography coupled to nuclear magnetic resonance with solid phase extraction (LC-SPE-NMR), and nuclear magnetic resonance (NMR)...
2019: Journal of Analytical Methods in Chemistry
José Herrera-Rodriguez, Aurora Signorazzi, Marijke Holtrop, Jacqueline de Vries-Idema, Anke Huckriede
The vast majority of commercially available inactivated influenza vaccines are produced from egg-grown or cell-grown live influenza virus. The first step in the production process is virus inactivation with β-propiolactone (BPL) or formaldehyde (FA). Recommendations for production of inactivated vaccines merely define the maximal concentration for both reagents, leaving the optimization of the process to the manufacturers. We assessed the effect of inactivation with BPL and FA on 5 different influenza virus strains...
February 11, 2019: Vaccine
Surekha K Satpute, Nishigandha S Mone, Parijat Das, Ibrahim M Banat, Arun G Banpurkar
BACKGROUND: Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903...
February 13, 2019: BMC Microbiology
Feng-Yi Lin, Kuo-Tsang Huang, Tzu-Ping Lin, Ruey-Lung Hwang
As urbanization expands and diversifies, weather data produced by a single weather station in a suburb are no longer adequate to represent and reflect microclimatic changes of a city. This study selected 34 automatic weather stations in Tainan City, Taiwan, to conduct temperature and humidity measurements over a period of one year. Based on those observed weather data and urban environment parameters obtained from a geographic information system, as well as morphing approach, this study constructed a method of generating hourly local weather data for urban areas while accounting for urban heat island (UHI) effect in summer...
February 25, 2019: Science of the Total Environment
Saptarshi Ghosh, Shuntaro Takahashi, Tamaki Endoh, Hisae Tateishi-Karimata, Soumitra Hazra, Naoki Sugimoto
Recent advancement in nucleic acid techniques inside cells demands the knowledge of the stability of nucleic acid structures in molecular crowding. The nearest-neighbor model has been successfully used to predict thermodynamic parameters for the formation of nucleic acid duplexes, with significant accuracy in a dilute solution. However, knowledge about the applicability of the model in molecular crowding is still limited. To determine and predict the stabilities of DNA duplexes in a cell-like crowded environment, we systematically investigated the validity of the nearest-neighbor model for Watson-Crick self-complementary DNA duplexes in molecular crowding...
February 8, 2019: Nucleic Acids Research
Shiming Song, Cuifang Zhang, Zhaojie Chen, Jie Wei, Huihua Tan, Xuesheng Li
Hydrolysis and photolysis of bentazone in abiotic aqueous solutions were examined under laboratory conditions. Hydrolysis was studied in different buffer solutions (pH 4.0 ± 0.1, 7.0 ± 0.1, and 9.0 ± 0.1), at different temperatures (15 °C ± 2 °C, 25 °C ± 2 °C, 35 °C ± 2 °C, and 45 °C ± 2 °C), and at different Fe3+ concentrations (1, 5, and 10 mg/L). Photolysis was assessed in different buffer solutions and at different solvent (methanol and ethyl acetate) concentrations (10%, 20%, and 30%) or Fe3+ (1, 5, and 10 mg/L) concentrations and under mercury or xenon light irradiation...
February 12, 2019: Environmental Science and Pollution Research International
Yun-Hsun Hsu, Jia-Jia Toh, Chiz-Tzung Chang, Mine-Yine Liu
A cyclodextrin-micellar electrokinetic chromatography (CD-MEKC) method has been developed to determine the apolipoproteins (apos) of human high-density lipoprotein (HDL). The optimal CD-MEKC conditions included a separation buffer mixture of 5 mM sodium phosphate, 40 mM bile salts (50% sodium cholate and 50% sodium deoxycholate), 25 mM carboxymethyl-β-CD (CM-β-CD) and pH 7.0. The separation voltage was 15 kV, and the capillary temperature was 15℃. The CD-MEKC profiles of human HDL apolipoproteins showed good repeatability and sensitivity...
January 28, 2019: Journal of Chromatography. A
Mirjana Malnar, Tadeja Režen
INTRODUCTION: FFPE samples represent a rich pool of tissue samples for retrospective analyses of mRNA and miRNA analyses. However, the initial formalin fixation introduces a chemical modification of RNA and causes its degradation, therefore, a longer storage of tissue in formalin is predicted to render ribonucleic acids lost for isolation and subsequent analyses. METHODS: Herein, we tested the impact of several factors on isolation of total RNA and detection with RT-qPCR from mouse liver tissue stored for over two years in formalin at room temperature...
February 6, 2019: Journal of Pharmacological and Toxicological Methods
Elizabeth A Ainsworth, Pauline Lemonnier, Jessica M Wedow
Human activities result in a wide array of pollutants being released to the atmosphere. A number of these pollutants have direct effects on plants, including carbon dioxide (CO2 ), which is the substrate for photosynthesis, and ozone (O3 ), a damaging oxidant. How plants respond to changes in these atmospheric air pollutants, both directly and indirectly, feeds back on atmospheric composition and climate, global net primary productivity and ecosystem service provisioning. Here we discuss the past, current and future trends in emissions of CO2 and O3 and synthesize the current atmospheric CO2 and O3 budgets, describing the important role of vegetation in determining the atmospheric burden of those pollutants...
February 7, 2019: Plant Biology
Pegah Salehian, Behzad Shareghi, Mansoore Hosseini-Koupaei
In this work, the effect of two organic polyamines (spermine and spermidine) on the fluorescence intensity and activity of bovine intestinal alkaline phosphatase (BIALP) are investigated. The interaction of BIALP with spermine and spermidine was studied in a diethanolamine buffer with 0.5 mM magnesium chloride (pH 9.8) and at two temperatures by using the fluorescence quenching method. Furthermore, the activity of enzyme was studied using UV-Vis spectrophotometry in a diethanolamine buffer with 0.5 mM magnesium chloride, at 37 °C, in the absence and presence of different concentrations of each polyamine (0-5 mM)...
February 7, 2019: Journal of Biological Physics
Tae-Jin Choi, Temesgen Tola Geletu
Recombinant flounder growth hormone was overproduced in E. coli by using codon optimized synthetic gene and optimized expression conditions for high level production. The gene was cloned into PET-28a expression vector and transformed into E. coli BL21 (DE3). Induction at lower temperature, lower IPTG concentrations and richer growth media during expression resulted in increased expression level. The protein expression profile was analyzed by SDS-PAGE, the authenticity was confirmed by western blotting and the concentration was determined by Bradford assay...
December 2018: Journal, Genetic Engineering & Biotechnology
Maria Paniw, Nino Maag, Gabriele Cozzi, Tim Clutton-Brock, Arpat Ozgul
Species in extreme habitats increasingly face changes in seasonal climate, but the demographic mechanisms through which these changes affect population persistence remain unknown. We investigated how changes in seasonal rainfall and temperature influence vital rates and viability of an arid environment specialist, the Kalahari meerkat, through effects on body mass. We show that climate change-induced reduction in adult mass in the prebreeding season would decrease fecundity during the breeding season and increase extinction risk, particularly at low population densities...
February 8, 2019: Science
Daoyong Yu, Jinxiao Lan, Naseer Ullah Khan, Quan Li, Fengxi Xu, Guihong Huang, Hai Xu, Fang Huang
Photosystem I (PSI) generates the most negative redox potential found in nature, and the performance of solar energy conversion into alternative energy sources in artificial systems highly depends on the thermal stability of PSI. Thus, understanding thermal denaturation is an important prerequisite for the use of PSI at elevated temperatures. To assess the thermal stability of surfactant-solubilized PSI from cyanobacteria Arthrospira Platensis, the synergistic denaturation effect of heat and surfactant was studied...
February 6, 2019: Photosynthesis Research
Rachel Miller, Trish Thorne-Nuzzo, Isabell Loftin, Abigail McElhinny, Penny Towne, June Clements
Diagnostic assays for molecular alterations highly correlated with prognosis, predictive efficacy or safety of therapeutics are valuable clinical tools and in some cases approved as companion diagnostics (CDx) by the Federal Food and Drug Administration. For example, assays that determine echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) translocation status have been approved as CDx assay for therapies that target this molecular alteration. Characterizing the parameters that may compromise diagnostic accuracy for molecular biomarkers is critical for optimal patient care...
February 4, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Weifu Guo
Scleractinian corals promote the precipitation of their carbonate skeleton by elevating the pH and dissolved inorganic carbon (DIC) concentration of their calcifying fluid above that of seawater. The fact corals actively regulate their calcifying fluid chemistry implies the potential for acclimation to ocean acidification. However, the extent to which corals can adjust their regulation mechanism in the face of decreasing ocean pH has not been rigorously tested. Here I present a numerical model simulating pH and DIC up-regulation by corals, and use it to determine the relative importance of physiological regulation versus seawater conditions in controlling coral calcifying fluid chemistry...
February 4, 2019: Scientific Reports
Giulia Vico, Danielle A Way, Vaughan Hurry, Stefano Manzoni
Under future climates, leaf temperature (Tl ) will be higher and more variable. This will affect plant carbon (C) balance because photosynthesis and respiration both respond to short-term (sub-daily) fluctuations in Tl and acclimate in the longer term (days to months). This study asks the question: to what extent can the potential and speed of photosynthetic acclimation buffer leaf C gain from rising and increasingly variable Tl ? We quantified how increases in the mean and variability of growth temperature affect leaf performance (mean net CO2 assimilation rates, Anet ; its variability; and time under near-optimal photosynthetic conditions), as mediated by thermal acclimation...
February 1, 2019: Plant, Cell & Environment
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