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Chaowei Shen, Jie Liu, Furong Zhu, Ruoqian Lei, Han Cheng, Chao Zhang, Xinmiao Sui, Liu Ding, Mei Yang, Hongbo Chen, Rui Ding, Jiyu Cao
BACKGROUND: Cooking oil fumes (COFs), a main pollutant in kitchen air, is a major risk to human health. In our previous research, exposure to COFs-derived PM2.5 could cause umbilical vascular endothelial dysfunction, leading to decreased fetal weight. Here, to test the role of ROS-mediated NLRP3 inflammasome pathway in blood vessel formation of human umbilical vein endothelial cells (HUVECs) caused by COFs-derived PM2.5 , the cells were exposed to COFs-derived PM2.5 at different concentrations with and without N-acetyl-L-cysteine (NAC)...
March 13, 2019: Ecotoxicology and Environmental Safety
Hongliang Zhang, Xiaojie Wei, Shunyu Lu, Xing Lin, Jianchun Huang, Lixiu Chen, Xiang Huang, Luhui Jiang, Yuchun Li, Luhui Qin, Jinbin Wei, Renbin Huang
BACKGROUND: Hyperglycemia stimulated epithelial-mesenchymal transition (EMT) plays a critical role in initiating and progressing renal fibrosis in diabetic kidney disease (DKD). It is crucial to explore novel renal protective drugs for the treatment of DKD. OBJECTIVE: The present study is to confirm our hypothesis and to accumulate the information for the application of DMDD (2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione) as a novel therapeutic agent to potentially inhibit renal fibrogenesis and EMT in the DKD...
March 13, 2019: Biomedicine & Pharmacotherapy
Xin Zhao, Fengjiao Du, Xiaolin Liu, Qiuyan Ruan, Zhulian Wu, Chuan Lei, Yanfei Deng, Chan Luo, Jianrong Jiang, Deshun Shi, Fenghua Lu
Brain-derived neurotrophic factor (BDNF) has been discovered and characterized for several decades, yet its expression pattern in non-neuronal tissues like ovary and potential mechanism during oocyte maturation are still poorly understood. Thus the present study was devised to determine the expression pattern and mechanism of BDNF during buffalo oocyte maturation. The results revealed that BDNF was presented at different stages of buffalo ovarian follicles as well as during oocyte maturation and early embryo development...
February 23, 2019: Theriogenology
Wanzheng Wang, Litao Wu, Xiaojuan Du, Fujun Zhang, Sayyed Hanif Ullah, Ting Lei, Dongming Li, Xiaofei Yan
Long-time consumption of high-fat food is a direct cause of cardiovascular diseases, and high-fat-related inflammation plays an important role in it. Toll-like receptors (TLRs), especially TLR2 and TLR4, play important roles in high-fat-related inflammation. However, the impact of TLR2 on high-fat-associated cardiovascular complications is still unknown. In this study, we try to investigate the relationship between TLR2 and high-fat-related cardiac injury. SD rats were allocated to either a control group which were fed with normal diet or a high-fat group which were fed with high-fat diet for 5 months...
March 16, 2019: Acta Biochimica et Biophysica Sinica
Landon Myer, Andrew D Redd, Elton Mukonda, Briana A Lynch, Tamsin K Phillips, Anna Eisenberg, Nei-Yuan Hsiao, Adam Capoferri, Alison Zerbe, William Clarke, Maia Lesosky, Autumn Breaud, James McIntyre, Daniel Bruno, Craig Martens, Elaine J Abrams, Steven J Reynolds
BACKGROUND: Elevated viral load (VL) early after antiretroviral therapy (ART) initiation appears frequently in pregnant and postpartum women living with HIV, however the relative contributions of pre-ART drug-resistant mutations (DRM) versus non-adherence in the aetiology of elevated VL are unknown. METHODS: Within a cohort of women initiating ART during pregnancy in Cape Town, South Africa, we compared women with elevated VL after initial suppression (cases, n=80) incidence-density matched to women who maintained suppression over time (controls, n=87)...
March 16, 2019: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Caroline Cayrou, Christopher D Bayliss
Reverse transcriptase quantitative PCR (RT qPCR) is widely used for assessing the levels of expression of specific genes in various organisms. Here we describe a RT qPCR assay designed to determine the level of expression of fHbp in multiple isolates of Neisseria meningitidis. The level of expression is measured by a two-step qPCR and is associated with a promoter region analysis.
2019: Methods in Molecular Biology
Francielle Liz Monteiro, Bruno Martins, Juliana F Cargnelutti, Jessica G Noll, Rudi Weiblen, Eduardo F Flores
Bovine pestiviruses, e.g., bovine viral diarrhea virus types 1 (BVDV-1 or Pestivirus A), BVDV-2 (Pestivirus B), and HoBi-like pestiviruses (HoBiPeV or Pestivirus H), have been shown to circulate in Brazilian cattle in varied proportions. In this study, we identified genetically pestiviruses circulating in beef cattle in Rio Grande do Sul, the southern most Brazilian state. Screening of serum of 15.584 beef calves destined to be export by an antigen capture ELISA and, subsequently, by reverse-transcription polymerase chain reaction (RT-PCR), revealed 135 containing pestivirus RNA...
March 15, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Carla M Barbosa, Edison L Durigon, Luciano M Thomazelli, Tatiana Ometto, Roberta Marcatti, Marcello Shiavo Nardi, Daniel M de Aguiar, João Batista Pinho, Maria Virginia Petry, Isaac Simão Neto, Patrícia Serafini, Roberta Costa Rodrigues, Severino Mendes de Azevedo Junior, Luiz Gustavo B Góes, Jansen de Araujo
Coronaviruses are single-stranded positive-sense RNA viruses associated with important avian diseases. Their relatively high rates of mutation and recombination frequencies allow them to adapt to new hosts and ecological niches. Although Brazil has 18% of global avian species diversity, studies regarding the presence of avian viral diseases in wild birds in South America are scarce. In this study, we performed a retrospective analysis of the presence of CoVs in 746 wild birds. Oropharyngeal and cloacal swabs were obtained and placed together in vials containing VTM transport medium collected in different regions of Brazil between 2006 and 2013...
March 15, 2019: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Weixuan Yu, Wen Deng, Qiang Zhao, Hongkai Zhuang, Chuanzhao Zhang, Zhixiang Jian
Hepatocellular carcinoma (HCC), the second common cancer, was a kind of primary liver cancer with high incidence. miR-501, identified as a novel regulator, was acted as a potential biomarker in several diseases. JDP2, acted as a repressor of AP-1 complex, was a member of the basic leucine zipper (bZIP) transcription factor family. RT-qPCR was applied to evaluate miR-501 and JDP2 expression level and we found that miR-501 was upregulated in HCC tissues and cells. miR-501 ectopic expression promoted HCC cell invasion and epithelial-mesenchymal transition (EMT), while low expression present the opposite results...
March 15, 2019: Human Cell
Chao Ye, Qingyuan Huang, Tingting Chen, Jiali Jiang, Fengqing Hou, Dongyi Xu, Yuanyi Peng, Rendong Fang, Jing Chen
Enzootic nasal adenocarcinoma (ENA) of goats, characterized by transformation of epithelial cells of the ethmoid turbinates, is caused by enzootic nasal tumor virus 2 (ENTV-2). ENTV-2 belongs to the genus Betaretrovirus and has extended its distribution globally with a high prevalence; however, the genetic diversity and genotypic distribution for ENTV-2 have not been analyzed systematically due to the limited availability of sequence data. In this study, an infection by ENTV-2 was detected by RT-PCR in Chongqing in July 2018, and the complete sequence of one strain (CQ1) was determined...
March 15, 2019: Archives of Virology
Toshio Kaneda, Yuki Nakajima, Sae Koshikawa, Alfarius Eko Nugroho, Hiroshi Morita
Previously, we reported that cyclolinopeptides (CLs) extracted from flaxseed inhibited receptor activator of nuclear factor κ-B ligand (RANKL)-induced osteoclastogenesis from mouse bone marrow cells in vitro. However, mode of action involved in CLs-inhibited osteoclastogenesis has been yet unknown. Therefore, in this study, we investigated the details of inhibitory activity of cyclolinopeptide-F (CL-F) in osteoclastogenesis, as a representative of CLs. CL-F dose-dependently inhibited RANKL-induced osteoclastogenesis (IC50 0...
March 15, 2019: Journal of Natural Medicines
Giulia Daniele, Alberto L'Abbate, Antonella Turchiano, Orazio Palumbo, Massimo Carella, Crocifissa Lo Cunsolo, Paolo Iuzzolino, Angelo Lonoce, María Hernández-Sánchez, Carla Minoia, Patrizia Leone, Jesus Maria Hernandez-Rivas, Clelia Tiziana Storlazzi
The identification of chromosome 1 translocations and deletions is a rare and poorly investigated event in chronic lymphocytic leukemia (CLL). Nevertheless, the identification of novel additional molecular alterations is of great interest, opening to new prognostic and therapeutic strategies for such heterogeneous hematological disease. We here describe a patient affected by CLL with a mutated IGHV status, showing a balanced t(1;3)(q23.1;q21.3) translocation and a der(18)t(1;18)(q24.2;p11.32), accompanying the recurrent 13q14 heterozygous deletion in all analyzed cells at onset...
March 15, 2019: Clinical and Experimental Medicine
Fengyuan Zhao, Xiaoqing Hu, Jiahao Zhang, Weili Shi, Bo Ren, Hongjie Huang, Yingfang Ao
PURPOSE: The purpose of this study was to evaluate whether a flattened bone tunnel has a positive effect on the tendon-bone healing (TBH) process in the early period after anterior cruciate ligament (ACL) reconstruction. METHODS: Seventy-two New Zealand White rabbits were randomly allocated into two groups, the flattened tunnel (FT) group and the conventional round tunnel (RT) group. We compared the cross-sectional areas and diameters of the bone tunnels between the two groups through computed tomography (CT) scanning...
March 14, 2019: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
Jiabao Hu, Qijun Le, Yajun Wang, Na Yu, Xiaohuan Cao, Siwen Kuang, Man Zhang, Weiwei Gu, Yibo Sun, Yang Yang, Shanliang Xu, Xiaojun Yan
Formaldehyde can effectively control ectoparasites in the silver pomfret (Pampus argenteus). However, there is limited information on the effects of formaldehyde treatment at a molecular level in the fish. In the present study, transcriptome profiling was conducted to investigate the effects of formaldehyde treatment (80 mg/L, bath for 1 h every day for three consecutive days) on the liver and kidney of silver pomfret. A total of 617959982 clean reads were obtained and assembled into 265760 unigenes with an N50 length of 1507 bp, and the assembled unigenes were all annotated by alignment with public databases...
March 12, 2019: Fish & Shellfish Immunology
Alexandru Szathmari, M Zerah, M Vinchon, C Dufour, E Gimbert, F Di Rocco, S Chabaud, C Conter, C Mottolese, D Frappaz
BACKGROUND: Ependymoma is the most frequent spinal tumor in adults but it is rather uncommon in children. The aim of this study is to summarize retrospectively clinical and therapeutic experience in the treatment of pediatric spinal ependymomas in France. MATERIAL AND METHODS: In this retrospective multicenter study, data from patients younger than 18 years old treated between 2000 and 2010 for spinal ependymomas were collected. Epidemiologic, tumor- and treatment-related data were analysed...
March 12, 2019: World Neurosurgery
Mathabo Ruth Lutu, Sanelisiwe Nzuza, Pascale Edith Mofo Mato, Khmera Govender, Londiwe Mphumelelo Gumede, Sinenhlanhla Innocentia Kumalo, Nontokozo Nomfundo Mlambo, Ranjendraparsad Hurchund, Peter Mark Oroma Owira
Nucleoside reverse transcriptase inhibitors (NRTIs) form the backbone in combination antiretroviral therapy (cARVs). They halt chain elongation of the viral cDNA by acting as false substrates in counterfeit incorporation mechanism to viral RNA-dependent DNA polymerase. In the process genomic DNA polymerase as well as mitochondrial DNA (mtDNA) polymerase-γ (which has a much higher affinity for these drugs at therapeutic doses) are also impaired. This leads to mitochondrial toxicity that manifests clinically as mitochondrial myopathy, peripheral neuropathy, hyperlactatemia or lactic acidosis and lipoatrophy...
March 12, 2019: European Journal of Pharmacology
Jie Wang, Lanyu Shen, Hong Hong, Jie Li, Hongtai Wang, Xiuzhen Li
Diabetic nephropathy (DN) is the most common complication of diabetes mellitus. Atrasentan (Atr) has potential therapeutic values for DN. MicroRNAs (miRNAs) function as vital regulators in the pathophysiology of kidney diseases including DN. Our present study aimed to further explore whether Atr could alleviate kidney injury by regulating microRNA-21(miR-21)/forkhead box O1 (FOXO1) in DN mouse models and cell models. Blood glucose concentration and ACR ratio were determined by matching commercial kits. MiR-21 and FOXO1 mRNA level was measured by RT-qPCR assay...
March 12, 2019: European Journal of Pharmacology
Muresan Ximena Maria, Narzt Marie-Sophie, Brittany Woodby, Francesca Ferrara, Gruber Florian, Valacchi Giuseppe
BACKGROUND: The main functions of the skin are to protect against environmental insults and prevent water loss, which are performed by the complex lipid- and protein matrix present in the outermost layers of the epithelium. The lipidome of these outer layers is mainly composed of ceramides, fatty acids, and cholesterol, which regulates keratinocyte differentiation and skin barrier function. SR-B1 is a multifunctional scavenger receptor that is best known for facilitating uptake of cholesterol from HDL particles in the liver, but it is also expressed in the skin...
March 12, 2019: Archives of Biochemistry and Biophysics
Mario Alejandro Lorenzetti, María Jimena Mosna, Elena Noemí De Matteo, Mercedes García Lombardi, Sandra Lorena Colli, María Victoria Preciado
Survivin is abundantly expressed during fetal development but absent in most differentiated adult tissues; an exception being components of the immune system, such as B and T lymphocytes. Beyond acting as a master regulator of the cell cycle, survivin acts as an inhibitor of apoptosis and is overexpressed in almost all carcinoma types; however, its expression in lymphomas is lesser-explored. Survivin's role in carcinogenesis was subjected to its sub-cellular localization and splice transcripts expression, namely wild-type survivin, survivin-∆Ex3 and survivin-2B...
March 12, 2019: Experimental and Molecular Pathology
Elisabeth Driessen, Deniece Detillon, Gerbern Bootsma, Dirk De Ruysscher, Eelco Veen, Mieke Aarts, Maryska Janssen-Heijnen
OBJECTIVES: Insights regarding utilization and survival of surgery and radiotherapy (stereotactic body radiotherapy (SBRT) or conventional radiotherapy (RT)) are lacking for older patients with stage I and II non-small cell lung cancer (NSCLC) in clinical practice. METHODS: Data from the Netherlands Cancer Registry were retrieved for patients ≥65 years with clinical stage I-II NSCLC in 2010-2015. Descriptive analyses, overall survival (OS), and cox regression were stratified for stage I (n = 8742) and II (n = 3439) and compared age groups (65-74 years vs ≥75 years)...
March 12, 2019: Journal of Geriatric Oncology
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