keyword
https://read.qxmd.com/read/38289251/key-structural-factors-and-intermolecular-interactions-underlying-the-formation-functional-properties-and-behaviour-in-the-gastrointestinal-tract-in-vitro-of-the-liposomal-form-of-nutraceuticals-coated-with-whey-proteins-and-chitosan
#21
JOURNAL ARTICLE
Maria G Semenova, Anna S Antipova, Elena I Martirosova, Nadezhda P Palmina, Daria V Zelikina, Sergey A Chebotarev, Natalya G Bogdanova, Maria S Anokhina, Valery V Kasparov
The aim of this study was to gain a better understanding of the key structural factors and intermolecular interactions underlying the formation, functionality, and in vitro gastrointestinal behaviour of the liposomal form of nutraceuticals coated with whey proteins (WPI) and chitosan (CHIT). Phosphatidylcholine (PC) liposomes were used to encapsulate a combination of hydrophobic and hydrophilic nutraceuticals. The hydrophobic constituents were long-chain (LC) n-3 PUFAs (DHA and EPA) from fish oil (FO), vitamin D3, and clove essential oil (CEO), while the hydrophilic component was γ-aminobutyric acid (GABA)...
January 30, 2024: Food & Function
https://read.qxmd.com/read/38288644/chitosan-coated-soybean-protein-isolate-lecithin-nanoparticles-for-enhancing-the-stability-and-bioaccessibility-of-phytosterol
#22
JOURNAL ARTICLE
Ming Cheng, Ye Tao, Chunwei Wang, Ao Li
BACKGROUND: Phytosterols (PS) have various beneficial effects on human health, especially the property of reducing blood cholesterol. However, the low solubility and bioaccessibility of PS have greatly limited their application in functional food ingredients. RESULTS: To improve the bioaccessibility and stability of PS, chitosan-coated PS nanoparticles (CS-PNP) were successfully prepared by self-assembly. The properties of CS-PNP, including size, zeta potential, encapsulation efficiency (EE) and loading amount (LA) were characterised...
January 30, 2024: Journal of the Science of Food and Agriculture
https://read.qxmd.com/read/38275611/first-case-of-a-dominant-de-novo-sec23a-mutation-with-neurological-and-psychiatric-features-new-insights-into-cranio-lenticulo-sutural-dysplasia-with-literature-review
#23
REVIEW
Elia Marco Paolo Minale, Alessandro De Falco, Emanuele Agolini, Antonio Novelli, Roberta Russo, Immacolata Andolfo, Achille Iolascon, Carmelo Piscopo
Cranio-lenticulo-sutural dysplasia (CLSD, OMIM #607812) is a rare genetic condition characterized by late-closing fontanels, skeletal defects, dysmorphisms, and congenital cataracts that are caused by bi-allelic or monoallelic variants in the SEC23A gene. Autosomal recessive inheritance (AR-CLSD) has been extensively documented in several cases with homozygous or compound heterozygous variants in SEC23A , whereas autosomal dominant inheritance (AD-CLSD) involving heterozygous inherited variants has been reported just in three patients...
January 20, 2024: Genes
https://read.qxmd.com/read/38267583/hiv-1-capsids-enter-the-fg-phase-of-nuclear-pores-like-a-transport-receptor
#24
JOURNAL ARTICLE
Liran Fu, Erika N Weiskopf, Onno Akkermans, Nicholas A Swanson, Shiya Cheng, Thomas U Schwartz, Dirk Görlich
HIV-1 infection requires nuclear entry of the viral genome. Previous evidence suggests that this entry proceeds through nuclear pore complexes (NPCs), with the 120 × 60 nm capsid squeezing through an approximately 60-nm-wide central channel1 and crossing the permeability barrier of the NPC. This barrier can be described as an FG phase2 that is assembled from cohesively interacting phenylalanine-glycine (FG) repeats3 and is selectively permeable to cargo captured by nuclear transport receptors (NTRs)...
January 24, 2024: Nature
https://read.qxmd.com/read/38259076/%C3%AE-1-3-glucan-recognition-by-acanthamoeba-castellanii-as-a-putative-mechanism-of-amoeba-fungal-interactions
#25
JOURNAL ARTICLE
Marina da Silva Ferreira, Diego de Souza Gonçalves, Susana Ruiz Mendoza, Gabriel Afonso de Oliveira, Bruno Pontes, Claudia Rodríguez-de la Noval, Leandro Honorato, Luis Felipe Costa Ramos, Fábio C S Nogueira, Gilberto B Domont, Arturo Casadevall, Leonardo Nimrichter, Jose Mauro Peralta, Allan J Guimaraes
In this study, we conducted an in-depth analysis to characterize potential Acanthamoeba castellanii ( Ac ) proteins capable of recognizing fungal β-1,3-glucans. Ac specifically anchors curdlan or laminarin, indicating the presence of surface β-1,3-glucan-binding molecules. Using optical tweezers, strong adhesion of laminarin- or curdlan-coated beads to Ac was observed, highlighting their adhesive properties compared to controls (characteristic time τ of 46.9 and 43.9 s, respectively). Furthermore, Histoplasma capsulatum ( Hc ) G217B, possessing a β-1,3-glucan outer layer, showed significant adhesion to Ac compared to a Hc G186 strain with an α-1,3-glucan outer layer (τ of 5...
January 23, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38253544/host-guest-mechanism-via-induced-fit-fullerene-complexation-in-porphin-receptor-to-probe-salivary-alpha-amylase-in-dental-caries-for-clinical-applications
#26
JOURNAL ARTICLE
Hira Asghar, Sehrish Bilal, Mian Hasnain Nawaz, Ghulam Rasool, Akhtar Hayat
Salivary α-amylase is the most abundant protein of human saliva that potentially binds to streptococcus and other bacteria via specific surface-exposed α-amylase-binding proteins and plays a significant role in caries development. The detection of α-amylase in saliva can be used as a bioindicator of caries development. Herein, a facile strategy has been applied, tailoring the photochemical properties of 5,10,15,20-tetrakis(4-hydroxyphenyl)-21 H ,23 H -porphine (TPPOH) and the fullerene C60 complex...
January 22, 2024: ACS Applied Bio Materials
https://read.qxmd.com/read/38227987/magnetically-navigated-microbubbles-coated-with-albumin-polyarginine-and-superparamagnetic-iron-oxide-nanoparticles
#27
JOURNAL ARTICLE
Olga I Gusliakova, Maxim A Kurochkin, Roman A Barmin, Ekaterina S Prikhozhdenko, Tatyana M Estifeeva, Polina G Rudakovskaya, Olga A Sindeeva, Victor V Galushka, Evgeny S Vavaev, Aleksei S Komlev, Evgeny V Lyubin, Andrey A Fedyanin, Krishna Kanti Dey, Dmitry A Gorin
While microbubbles (MB) are routinely used for ultrasound (US) imaging, magnetic MB are increasingly explored as they can be guided to specific sites of interest by applied magnetic field gradient. This requires the MB shell composition tuning to prolong MB stability and provide functionalization capabilities with magnetic nanoparticles. Hence, we developed air-filled MB stabilized by a protein-polymer complex of bovine serum albumin (BSA) and poly-L-arginine (pArg) of different molecular weights, showing that pArg of moderate molecular weight distribution (15-70 kDa) enabled MB with greater stability and acoustic response while preserving MB narrow diameters and the relative viability of THP-1 cells after 48 h of incubation...
January 9, 2024: Biomater Adv
https://read.qxmd.com/read/38214784/increasing-hba1c-is-associated-with-reduced-cd8-t-cell-functionality-in-response-to-influenza-virus-in-a-tcr-dependent-manner-in-individuals-with-diabetes-mellitus
#28
JOURNAL ARTICLE
Katina D Hulme, Zhen Wei Marcus Tong, Louise C Rowntree, Carolien E van de Sandt, Katharina Ronacher, Emma J Grant, Emily S Dorey, Linda A Gallo, Stephanie Gras, Katherine Kedzierska, Helen L Barrett, Kirsty R Short
Diabetes mellitus is on the rise globally and is a known susceptibility factor for severe influenza virus infections. However, the mechanisms by which diabetes increases the severity of an influenza virus infection are yet to be fully defined. Diabetes mellitus is hallmarked by high glucose concentrations in the blood. We hypothesized that these high glucose concentrations affect the functionality of CD8+ T cells, which play a key role eliminating virus-infected cells and have been shown to decrease influenza disease severity...
January 12, 2024: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/38206473/oxali-palladium-nanoparticle-synthesis-characterization-protein-binding-and-apoptosis-induction-in-colorectal-cancer-cells
#29
JOURNAL ARTICLE
Nasim Golestannezhad, Adeleh Divsalar, Farideh Badalkhani-Khamseh, Milad Rasouli, Arefeh Seyedarabi, Behafarid Ghalandari, Xianting Ding, Fatemeh Goli, Sander Bekeschus, Ali Akbar Moosavi Movahedi, Mahboube Eslami Moghadam
This paper focuses on the synthesis of nano-oxali-palladium coated with turmeric extract (PdNPs) using a green chemistry technique based on the reduction in the Pd (II) complex by phytochemicals inherent in turmeric extract. PdNPs were examined and characterized using Field Emission Scanning Electron Microscopy (FESEM), Dynamic Light Scattering (DLS), Fourier Transform Infrared (FTIR), and Atomic Force Microscopy (AFM). Using different spectroscopic and molecular dynamics simulations, a protein-binding analysis of the produced nanoparticle was conducted by observing its interaction with human serum albumin (HSA)...
January 11, 2024: Journal of Materials Science. Materials in Medicine
https://read.qxmd.com/read/38203512/copi-vesicle-disruption-inhibits-mineralization-via-mtorc1-mediated-autophagy
#30
JOURNAL ARTICLE
Jiaming Nie, Shaoyang Ma, Yuchen Zhang, Shuchen Yu, Jiajia Yang, Ang Li, Dandan Pei
Bone mineralization is a sophisticated regulated process composed of crystalline calcium phosphate and collagen fibril. Autophagy, an evolutionarily conserved degradation system, whereby double-membrane vesicles deliver intracellular macromolecules and organelles to lysosomes for degradation, has recently been shown to play an essential role in mineralization. However, the formation of autophagosomes in mineralization remains to be determined. Here, we show that Coat Protein Complex I (COPI), responsible for Golgi-to-ER transport, plays a pivotal role in autophagosome formation in mineralization...
December 26, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38201709/single-component-hydrophilic-terpolymer-thin-film-systems-for-imparting-surface-chemical-versatility-on-various-substrates
#31
JOURNAL ARTICLE
Yun Hee Ko, Hai Ha Tran Nguyen, Christopher R Branstetter, Soeun Park, Jin-Kyun Lee, Jaesung Yang, Jangwook P Jung, Myungwoong Kim
We demonstrate a single-component hydrophilic photocrosslinkable copolymer system that incorporates all critical functionalities into one chain. This design allows for the creation of uniform functional organic coatings on a variety of substrates. The copolymers were composed of a poly(ethylene oxide)-containing monomer, a monomer that can release a primary amine upon UV light, and a monomer with reactive epoxide or cyclic dithiocarbonate with a primary amine. These copolymers are easily incorporated into the solution-casting process using polar solvents...
December 21, 2023: Polymers
https://read.qxmd.com/read/38198557/structural-basis-for-nuclear-import-of-hepatitis-b-virus-hbv-nucleocapsid-core
#32
JOURNAL ARTICLE
Ruoyu Yang, Ying-Hui Ko, Fenglin Li, Ravi K Lokareddy, Chun-Feng David Hou, Christine Kim, Shelby Klein, Santiago Antolínez, Juan F Marín, Carolina Pérez-Segura, Martin F Jarrold, Adam Zlotnick, Jodi A Hadden-Perilla, Gino Cingolani
Nuclear import of the hepatitis B virus (HBV) nucleocapsid is essential for replication that occurs in the nucleus. The ~360-angstrom HBV capsid translocates to the nuclear pore complex (NPC) as an intact particle, hijacking human importins in a reaction stimulated by host kinases. This paper describes the mechanisms of HBV capsid recognition by importins. We found that importin α1 binds a nuclear localization signal (NLS) at the far end of the HBV coat protein Cp183 carboxyl-terminal domain (CTD). This NLS is exposed to the capsid surface through a pore at the icosahedral quasi-sixfold vertex...
January 12, 2024: Science Advances
https://read.qxmd.com/read/38165727/a-label-free-aptamer-based-colorimetric-biosensor-for-rapid-gliadin-detection-in-foods-a-focus-on-pasta-bread-and-cookies
#33
JOURNAL ARTICLE
Seung Hwan Ham, Eunghee Kim, Hyebin Han, Min Gyu Lee, Young Jin Choi, Jungwoo Hahn
Despite numerous advancements in gluten detection, a substantial need remains for innovative, cost-effective, in situ methods that can be employed without complex analytical instruments. Addressing this demand, this study introduces a pioneering label-free colorimetric biosensor for the in situ detection of gliadin, a major component of gluten, which is a prevalent trigger of food allergies. Our novel approach employs the strategic coating of gold nanoparticles (AuNP) with gliadin-specific aptamers. In the absence of gliadin, these aptamers stably disperse AuNP, preventing their aggregation...
January 2, 2024: Analytical Methods: Advancing Methods and Applications
https://read.qxmd.com/read/38157306/quantitative-detection-of-biological-nanovesicles-in-drops-of-saliva-using-microcantilevers
#34
JOURNAL ARTICLE
Clodomiro Cafolla, James Philpott-Robson, Aaron Elbourne, Kislon Voïtchovsky
Extracellular nanovesicles (EVs) are lipid-based vesicles secreted by cells and are present in all bodily fluids. They play a central role in communication between distant cells and have been proposed as potential indicators for the early detection of a wide range of diseases, including different types of cancer. However, reliable quantification of a specific subpopulation of EVs remains challenging. The process is typically lengthy and costly and requires purification of relatively large quantities of biopsy samples...
December 29, 2023: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38139079/-arabidopsis-sec13b-interacts-with-suppressor-of-frigida-4-to-repress-flowering
#35
JOURNAL ARTICLE
Yanqi Yang, Hao Tian, Chunxue Xu, Haitao Li, Yan Li, Haitao Zhang, Biaoming Zhang, Wenya Yuan
SECRETORY13 (SEC13) is an essential member of the coat protein complex II (COPII), which was reported to mediate vesicular-specific transport from the endoplasmic reticulum (ER) to the Golgi apparatus and plays a crucial role in early secretory pathways. In Arabidopsis , there are two homologous proteins of SEC13: SEC13A and SEC13B. SUPPRESSOR OF FRIGIDA 4 ( SUF4 ) encodes a C2H2-type zinc finger protein that inhibits flowering by transcriptionally activating the FLOWERING LOCUS C ( FLC ) through the FRIGIDA (FRI) pathway in Arabidopsis ...
December 8, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38066006/nutrient-deprivation-alters-the-rate-of-copii-subunit-recruitment-at-er-subdomains-to-tune-secretory-protein-transport
#36
JOURNAL ARTICLE
William Kasberg, Peter Luong, Kevin A Swift, Anjon Audhya
Co-assembly of the multilayered coat protein complex II (COPII) with the Sar1 GTPase at subdomains of the endoplasmic reticulum (ER) enables secretory cargoes to be concentrated efficiently within nascent transport intermediates, which subsequently deliver their contents to ER-Golgi intermediate compartments. Here, we define the spatiotemporal accumulation of native COPII subunits and secretory cargoes at ER subdomains under differing nutrient availability conditions using a combination of CRISPR/Cas9-mediated genome editing and live cell imaging...
December 8, 2023: Nature Communications
https://read.qxmd.com/read/38062730/the-jagged-1-notch1-signalling-pathway-promotes-the-construction-of-tissue-engineered-heart-valves
#37
JOURNAL ARTICLE
Kang Liu, Zhang-Yan Wei, Xue-Hong Zhong, Xiaomei Liu, Hailong Chen, Yiyun Pan, Wen Zeng
BACKGROUND: Tissue-engineered heart valves (TEHVs) are promising new heart valve substitutes for valvular heart disease. The Notch signalling pathway plays a critical role in the development of congenital heart valves. OBJECTIVE: To investigate the role of the Notch signalling pathway in the construction of TEHVs. METHODS: The induced endothelial cells, which act as seed cells, were differentiated from adipose-derived stem cells and were treated with Jagged-1 (JAG-1) protein and γ-secretase inhibitor (DAPT, N-[N-(3,5-difluorophenacetyl)-l-alanyl]-s-phenylglycine t-butyl ester) respectively...
December 7, 2023: Tissue Engineering. Part A
https://read.qxmd.com/read/38057304/atg18-oligomer-organization-in-assembled-tubes-and-on-lipid-membrane-scaffolds
#38
JOURNAL ARTICLE
Daniel Mann, Simon A Fromm, Antonio Martinez-Sanchez, Navin Gopaldass, Ramona Choy, Andreas Mayer, Carsten Sachse
Autophagy-related protein 18 (Atg18) participates in the elongation of early autophagosomal structures in concert with Atg2 and Atg9 complexes. How Atg18 contributes to the structural coordination of Atg2 and Atg9 at the isolation membrane remains to be understood. Here, we determined the cryo-EM structures of Atg18 organized in helical tubes, Atg18 oligomers in solution as well as on lipid membrane scaffolds. The helical assembly is composed of Atg18 tetramers forming a lozenge cylindrical lattice with remarkable structural similarity to the COPII outer coat...
December 6, 2023: Nature Communications
https://read.qxmd.com/read/38044464/the-radiological-and-histological-phenotype-of-skeletal-abnormalities-in-fetal-arcn1-related-syndrome
#39
JOURNAL ARTICLE
Charlotte A Houck, Marije Koopmans, Peter G J Nikkels
Mutations in ARCN1 give rise to a syndromic disorder with rhizomelic short stature with microretrognathia and developmental delay. ARCN1 encodes the delta subunit of the coat protein I complex, which is required for intracellular trafficking of collagen 1 and which may also be involved in the endoplasmic reticulum (ER) stress response. In this paper we describe for the first time the skeletal histological abnormalities in an 18-week-old fetus with an ARCN1 mutation, and we suggest that the skeletal phenotype in ARCN1 -related syndrome has more resemblance with ER stress than with a defect in collagen 1 metabolism...
December 3, 2023: Pediatric and Developmental Pathology
https://read.qxmd.com/read/38041875/pyrogenic-and-inflammatory-mediators-are-produced-by-polarized-m1-and-m2-macrophages-activated-with-d-dimer-and-sars-cov-2-spike-immune-complexes
#40
JOURNAL ARTICLE
Yun-Jong Park, David Acosta, Mohammad Rubel Hoq, Surender Khurana, Hana Golding, Marina Zaitseva
Lung macrophages are the first line of defense against invading respiratory pathogens including SARS-CoV-2, yet activation of macrophage in the lungs can lead to hyperinflammatory immune response seen in severe COVID-19. Here we used human M1 and M2 polarized macrophages as a surrogate model of inflammatory and regulatory macrophages and explored whether immune complexes (IC) containing spike-specific IgG can trigger aberrant cytokine responses in macrophages in the lungs and associated lymph nodes. We show that IC of SARS-CoV-2 recombinant S protein coated with spike-specific monoclonal antibody induced production of Prostaglandin E2 (PGE2) in non-polarized (M0) and in M1 and M2-type polarized human macrophages only in the presence of D-dimer (DD), a fibrinogen degradation product, associated with coagulopathy in COVID-19...
December 1, 2023: Cytokine
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