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Coat Protein Complex 1

Huajun Zhou, Giancarlo Costaguta, Gregory S Payne
Clathrin coats drive transport vesicle formation from the plasma membrane and in pathways between the trans-Golgi network (TGN) and endosomes. Clathrin adaptors play central roles orchestrating assembly of clathrin coats. The yeast clathrin adaptor-interacting protein Irc6 is an orthologue of human p34, which is mutated in the inherited skin disorder punctate palmoplantar keratoderma type I. Irc6 and p34 bind to clathrin adaptor complexes AP-1 and AP-2 and are members of a conserved family characterized by a two-domain architecture...
March 14, 2019: Scientific Reports
Courtney Olsen, Kimiya Memarzadeh, Arzu Ulu, Heather S Carr, Andrew J Bean, Jeffrey A Frost
The Gi-coupled somatostatin receptor 2 (SST2) is a G-protein coupled receptor (GPCR) that mediates many of somatostatin's neuroendocrine actions. Upon stimulation, SST2 is rapidly internalized and transported to early endosomes before being recycled to the plasma membrane. However, little is known about the intracellular itinerary of SST2 after it moves to the early endosomal compartment, or the cytoplasmic proteins that regulate its trafficking. As Postsynaptic density protein/Discs large-1/Zona occludens-1 (PDZ) domain interactions often regulate the trafficking and signaling potential of other GPCRs, we examined the role of the SST2 PDZ ligand and additional C-terminal residues in controlling its intracellular trafficking...
March 1, 2019: Endocrinology
Haiyan Yan, Linlin Ma, Huiqiang Wang, Shuo Wu, Hua Huang, Zhengyi Gu, Jiandong Jiang, Yuhuan Li
Influenza is an acute transmissible respiratory infectious disease in humans and animals with high morbidity and mortality. It was reported that luteolin, extracted from Chinese herbs, could potently inhibit influenza virus replication in vitro. To assess the effect and explore the fundamental mechanism of luteolin, we infected several cell lines with two subtypes of influenza A virus (IAV), including A/Jiangxi/312/2006 (H3N2) and A/Fort Monmouth/1/1947 (H1N1) and demonstrated that luteolin suppressed the replication of IAV by cytopathic effect reduction method, qRT-PCR, immunofluorescence and Western blot assays...
February 13, 2019: Journal of Natural Medicines
Jiaxi Wang, Jie Li, Guoquan Yan, Mingxia Gao, Xiangmin Zhang
The highly effective analysis of glycopeptides from complex biological samples is an attractive and critical topic all the time. In this study, a novel thickness-controlled hydrophilic Mg-metal organic frameworks (Mg-MOFs) coating-functionalized magnetic graphene composite (MagG@Mg-MOFs-1C) was prepared for the capture of the glycopeptides. The as-synthesized composite exhibits an ultralow limit of detection (0.1 fmol μL-1), a perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 500 : 500, w/w/w), and a high binding capacity (150 mg g-1), satisfying reusability and high recovery in the recognition of glycopeptides due to its outstanding characteristics including strong magnetic property, large surface area (617 m2 g-1), plenty of affinity sites, and excellent hydrophilicity...
February 11, 2019: Nanoscale
Carolina V Uliana, Tássia R de Oliveira, Márcia R Cominetti, Ronaldo C Faria
The evaluation of interaction between small molecules and protein is an important step in the discovery of new drugs and to study complex biological systems. In this work, an alternative method was presented to evaluate small-molecule-protein interaction by using ligand capture by protein-coated magnetic particles (MPs) and disposable electrochemical cells. The interaction study was conducted using [10]-gingerol from ginger rhizome and a transmembrane protein αVβ3 integrin. Initially, the electrochemical behavior of the natural compound [10]-gingerol was evaluated with the disposable carbon-based electrodes and presented an irreversible oxidation process controlled by diffusion...
February 9, 2019: Analytical and Bioanalytical Chemistry
Thomas L Moore, Dominic A Urban, Laura Rodriguez-Lorenzo, Ana Milosevic, Federica Crippa, Miguel Spuch-Calvar, Sandor Balog, Barbara Rothen-Rutishauser, Marco Lattuada, Alke Petri-Fink
As a highly interdisciplinary field, working with nanoparticles in a biomedical context requires a robust understanding of soft matter physics, colloidal behaviors, nano-characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out to understand how in vitro technique, specifically the way we administer particles in 2D culture, can influence experimental outcomes. Gold nanoparticles coated with poly(vinylpyrrolidone) were added to J774A...
January 29, 2019: Scientific Reports
Kayesh M Ashraf, Chenyu Wang, Sithara S Nair, Kenneth J Wynne
Platinum cure for PDMS coatings on a thermal gradient (45 - 140 °C) was carried out to study the effect of temperature on surface chemistry and wetting behavior. Motivation was interest in surfaces with continuous gradients in wettability for applications such as protein adsorption, controlling bacterial adhesion, directional movement of cells, and biosensors. The Wilhelmy plate method and the advancing/receding drop method were employed for determining the positional dependence of θA and θR . A strong dependence of receding contact angles (R) on cure temperature was found for Sylgard 184 (S-PDMS) and a Pt-cured laboratory prepared analog (Pt PDMS) of known composition...
January 25, 2019: Langmuir: the ACS Journal of Surfaces and Colloids
Flávia A Belato, Carlos G Schrago, Christopher J Coates, Kenneth M Halanych, Elisa M Costa-Paiva
Multicellular organisms depend on oxygen-carrying proteins to transport oxygen throughout the body, therefore proteins such as hemoglobins (Hbs), hemocyanins (Hcs) and hemerythrins (Hrs) are essential for maintenance of tissues and cellular respiration. Vertebrate hemoglobins are among the most extensively studied proteins, however, much less is known about invertebrate hemoglobins. Recent studies of Hcs and Hrs have demonstrated they have much wider distributions than previously thought, suggesting that oxygen-binding protein diversity is underestimated across metazoans...
January 21, 2019: Genome Biology and Evolution
Brecht Guillemyn, Hülya Kayserili, Lynn Demuynck, Patrick Sips, Anne De Paepe, Delfien Syx, Paul J Coucke, Fransiska Malfait, Sofie Symoens
The cyclic adenosine monophosphate (AMP) responsive element binding protein 3-like 1 (CREB3L1) gene codes for the endoplasmic reticulum stress transducer old astrocyte specifically induced substance (OASIS), which has an important role in osteoblast differentiation during bone development. Deficiency of OASIS is linked to a severe form of autosomal recessive osteogenesis imperfecta (OI), but only few patients have been reported. We identified the first homozygous pathogenic missense variant (p.(Ala304Val)) in a patient with lethal OI, which is located within the highly conserved basic leucine zipper domain, four amino acids upstream of the DNA binding domain...
January 16, 2019: Human Molecular Genetics
Silke Kerruth, Catherine Coates, Céline D Dürst, Thomas G Oertner, Katalin Török
Genetically-encoded calcium indicators (GECIs) are useful reporters of cell signalling, neuronal and network activities. We have generated novel fast variants and investigated the kinetic mechanisms of two recently developed red-fluorescent GECIs (RGECIs), mApple-based jRGECO1a and mRuby-based jRCaMP1a. In the formation of fluorescent jRGECO1a and jRCaMP1a complexes calcium binding is followed by rate-limiting isomerisation. However fluorescence decay of calcium-bound jRGECO1a follows a different pathway form its formation: dissociation of calcium occurs first followed by the peptide, similarly to GCaMP-s...
January 16, 2019: Journal of Biological Chemistry
Sara Palchetti, Damiano Caputo, Luca Digiacomo, Anna Laura Capriotti, Roberto Coppola, Daniela Pozzi, Giulio Caracciolo
Pancreatic ductal adenocarcinoma (PDAC) is the fourth cause of cancer-related mortality in the Western world and is envisaged to become the second cause by 2030. Although our knowledge about the molecular biology of PDAC is continuously increasing, this progress has not been translated into better patients' outcome. Liposomes have been used to circumvent concerns associated with the low efficiency of anticancer drugs such as severe side effects and damage of healthy tissues, but they have not resulted in improved efficacy as yet...
January 15, 2019: Pharmaceutics
Emile Levy, Pierre Poinsot, Schohraya Spahis
PURPOSE OF REVIEW: Chylomicron retention disease (CRD) is an autosomic recessive disorder, in which intestinal fat malabsorption is the main cause of diverse severe manifestations. The specific molecular defect was identified in 2003 and consists of mutations in the SAR1B or SARA2 gene encoding for intracellular SAR1B GTPase protein. The aim of this review is first to provide an update of the recent biochemical, genetic and clinical findings, and second to discuss novel mechanisms related to hallmark symptoms...
January 11, 2019: Current Opinion in Lipidology
Megan J Agajanian, Matthew P Walker, Alison D Axtman, Roberta R Ruela-de-Sousa, D Stephen Serafin, Alex D Rabinowitz, David M Graham, Meagan B Ryan, Tigist Tamir, Yuko Nakamichi, Melissa V Gammons, James M Bennett, Rafael M Couñago, David H Drewry, Jonathan M Elkins, Carina Gileadi, Opher Gileadi, Paulo H Godoi, Nirav Kapadia, Susanne Müller, André S Santiago, Fiona J Sorrell, Carrow I Wells, Oleg Fedorov, Timothy M Willson, William J Zuercher, Michael B Major
β-Catenin-dependent WNT signal transduction governs development, tissue homeostasis, and a vast array of human diseases. Signal propagation through a WNT-Frizzled/LRP receptor complex requires proteins necessary for clathrin-mediated endocytosis (CME). Paradoxically, CME also negatively regulates WNT signaling through internalization and degradation of the receptor complex. Here, using a gain-of-function screen of the human kinome, we report that the AP2 associated kinase 1 (AAK1), a known CME enhancer, inhibits WNT signaling...
January 2, 2019: Cell Reports
Chenchun Weng, Joanna Kosalka, Ahmet C Berkyurek, Przemyslaw Stempor, Xuezhu Feng, Hui Mao, Chenming Zeng, Wen-Jun Li, Yong-Hong Yan, Meng-Qiu Dong, Natalia Rosalía Morero, Cecilia Zuliani, Orsolya Barabas, Julie Ahringer, Shouhong Guang, Eric A Miska
Piwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and nonself nucleic acids and maintain genome integrity and are essential for fertility in a variety of organisms. In Caenorhabditis elegans , most piRNA precursors are transcribed from two genomic clusters that contain thousands of individual piRNA transcription units. While a few genes have been shown to be required for piRNA biogenesis, the mechanism of piRNA transcription remains elusive. Here we used functional proteomics approaches to identify an upstream sequence transcription complex (USTC) that is essential for piRNA biogenesis...
January 1, 2019: Genes & Development
Caroline J Coats, Wendy E Heywood, Alex Virasami, Nadia Ashrafi, Petros Syrris, Cris Dos Remedios, Thomas A Treibel, James C Moon, Luis R Lopes, Christopher G A McGregor, Michael Ashworth, Neil J Sebire, William J McKenna, Kevin Mills, Perry M Elliott
BACKGROUND: Hypertrophic cardiomyopathy (HCM) is characterized by a complex phenotype that is only partly explained by the biological effects of individual genetic variants. The aim of this study was to use proteomic analysis of myocardial tissue to explore the postgenomic phenotype. METHODS: Label-free proteomic analysis was used initially to compare protein profiles in myocardial samples from 11 patients with HCM undergoing surgical myectomy with control samples from 6 healthy unused donor hearts...
December 2018: Circulation. Genomic and Precision Medicine
Ji Sun Park, Se Won Yi, Hye Jin Kim, Hyun Jyung Oh, Jung Sun Lee, Minyeon Go, Sung Han Shim, Keun-Hong Park
Background: For many years, researchers have sought to overcome major challenges in the use of nanoparticles as therapeutics, including issues related to intracellular delivery, biocompatibility, and activation. In particular, the genetic stability of cells treated with nanoparticles has become increasingly important in the context of stem cell therapy. Methods: Functional nanoparticles (Sunflower typed nanoparticles; SF-NPs) were fabricated by coating heparin pluronic F127 gels with quantum dot nanoparticles (QDs), and then bound the SOX9 gene to the QD nanogels...
2018: Theranostics
Jing Wang, Will Anderson, Junrong Li, Lynlee L Lin, Yuling Wang, Matt Trau
The colloidal stability of surface-enhanced Raman scattering (SERS) nanotags (Raman reporter-conjugated plasmonic nanoparticles) significantly affects the accuracy and reproducibility of SERS measurements, particularly in biological systems. Limited understanding of SERS nanotag stability may partly hamper the translation of SERS nanotags from the laboratory to their use in the clinic. In this contribution, we utilized differential centrifugal sedimentation (DCS), a reliable and straightforward technique to comprehensively analyze the colloidal stability of SERS nanotags in biological systems...
November 12, 2018: Journal of Colloid and Interface Science
Jung Mi Lim, Sujin Park, Mi-Sook Lee, Tamas Balla, Dongmin Kang, Sue Goo Rhee
Phosphatidylinositol 4-phosphate [PtdIns(4)P] plays a key role in the biogenesis of transport vesicles at the Golgi complex by recruiting coat proteins and their accessory factors. The PtdIns(4)P content of the Golgi is determined by the concerted action of PtdIns 4-kinase (PI4K) and PtdIns(4)P phosphatase enzymes. Sac1 (suppressor of actin 1) is the major PtdIns(4)P phosphatase and is localized to the Golgi and endoplasmic reticulum. The targeting of both PI4Ks and Sac1 to the Golgi membrane is extensively regulated, as is the catalytic activity of PI4Ks at the Golgi...
November 16, 2018: Free Radical Biology & Medicine
Aaron P Roznowski, Robert J Young, Samuel D Love, Avenetti A Andromita, Vanessa A Guzman, Margaret H Wilch, Ava Block, Anne McGill, Martine Lavelle, Anastasia Romanova, Aimi Sekiguchi, Meixiao Wang, April D Burch, Bentley A Fane
Although microviruses do not possess a visible tail structure, one vertex rearranges after interacting with host lipopolysaccharides. Most examinations of host range, eclipse, and penetration were conducted before this "host-induced" unique vertex was discovered and before DNA sequencing became routine. Consequently, structure-function relationships dictating host range remain undefined. Biochemical and genetic analyses were conducted with two closely related microviruses, α3 and ST-1. Despite ∼90% amino acid identity, the natural host of α3 is E...
November 14, 2018: Journal of Virology
Yuan Ding, Xiude Hua, Mei Du, Qian Yang, Lina Hou, Limin Wang, Fengquan Liu, Gualberto Gonzalez-Sapienza, Minghua Wang
Peptidomimetic and anti-immunocomplex peptides, which can be readily isolated from a phage-display library, have shown great potential for small-molecule immunoassay development because they typically improve the sensitivity and avoid the use of chemical haptens as coatings or tracer antigens. However, phage-borne peptides are unconventional immunoassay reagents, which greatly limits their use in commercial applications, and require secondary reagents for detection. In order to overcome these limitations, we used C2-15, a peptidomimetic of imidaclothiz, as a model peptide fused to emerald-green fluorescent protein (EmGFP) at the N-terminus (C2-15-EmGFP) and C-terminus (EmGFP-C2-15) to generate novel fluorescent-peptide tracers...
November 13, 2018: Analytical Chemistry
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