keyword
https://read.qxmd.com/read/38532056/inter-coat-protein-loading-of-active-ingredients-into-tobacco-mild-green-mosaic-virus-through-partial-dissociation-and-reassembly-of-the-virion
#1
JOURNAL ARTICLE
Ivonne González-Gamboa, Adam A Caparco, Justin McCaskill, Paulina Fuenlabrada-Velázquez, Samuel S Hays, Zhicheng Jin, Jesse V Jokerst, Jonathan K Pokorski, Nicole F Steinmetz
Chemical pesticide delivery is a fundamental aspect of agriculture. However, the extensive use of pesticides severely endangers the ecosystem because they accumulate on crops, in soil, as well as in drinking and groundwater. New frontiers in nano-engineering have opened the door for precision agriculture. We introduced Tobacco mild green mosaic virus (TMGMV) as a viable delivery platform with a high aspect ratio and favorable soil mobility. In this work, we assess the use of TMGMV as a chemical nanocarrier for agriculturally relevant cargo...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38518559/urinary-dengue-ns1-detection-on-au-decorated-zno-nanowire-platform
#2
JOURNAL ARTICLE
Kannika Sitthisuwannakul, Ratchanon Sukthai, Zetao Zhu, Kazuki Nagashima, Kunanon Chattrairat, Supranee Phanthanawiboon, Annop Klamchuen, Sakon Rahong, Yoshinobu Baba, Takao Yasui
Biodetection for non-invasive diagnostics of fluids, especially urine, remains a challenge to scientists due to low target concentrations. And biological complexes of the detection target may contain contaminants that also interfere with any assay. Dengue non-structural 1 protein (Dengue NS1) is an important biomarker for dengue hemorrhagic fever and dengue shock syndrome. Here, we developed an Au-decorated nanowire platform and applied it with a sandwich fluorophore-linked immunosorbent well plate assay (FLISA) to detect Dengue NS1 in urine...
March 13, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38502246/bifunctional-mnps-uio-66-arg-core-shell-satellite-nanocomposites-for-enrichment-of-phosphopeptides
#3
JOURNAL ARTICLE
Yuxiu Zhang, Nan Li, Jianru Li, Miao Fan, Qiqi Zhang, Fuquan Dang
A facile and mild method based on self-assembled lysozyme (LYZ) to fabricate bifunctional MNPs@UIO-66-Arg core-shell-satellite nanocomposites (CSSNCs) is reported for the high-efficiency enrichment of phosphopeptides. Under physiological conditions, LYZ rapidly self-assembled into a robust coating on Fe3 O4 @SiO2 magnetic nanoparticles (MNPs) with abundant surface functional groups, which effectively mediate heterogeneous nucleation and growth of UIO-66 nanocrystals. Well-defined MNPs@UIO-66 CSSNCs with stacked pores, showing high specific surface area (333...
March 19, 2024: Mikrochimica Acta
https://read.qxmd.com/read/38495999/a-prototype-of-ultrasensitive-time-resolved-fluoroimmunoassay-for-the-quantitation-of-lead-in-plasma-using-a-fluorescence-enhanced-europium-chelate-label-for-the-detection-system
#4
JOURNAL ARTICLE
Ibrahim A Darwish, Kenzi Suzuki, Hiroshi Ogawa, Zongzhi Wang
This study describes the prototype of a novel ultra-sensitive time-resolved fluoroimmunoassay (TRFIA) for the quantification of lead (Pb) in plasma. The assay procedures were conducted in 96-microwell plates and involved the competitive binding format. The assay used a mouse monoclonal antibody, designated as 2C33, that specifically recognized the diethylenetriamine pentaacetic acid chelate of Pb (Pb-DTPA) but did not recognize Pb-free DTPA chelator. The antigen used for coating onto the inner surfaces of assay plate microwells was Pb-DTPA conjugated with bovine serum albumin protein (Pb-DTPA-BSA)...
March 14, 2024: RSC Advances
https://read.qxmd.com/read/38490996/tango6-regulates-cell-proliferation-via-copi-vesicle-mediated-rpb2-nuclear-entry
#5
JOURNAL ARTICLE
Zhi Feng, Shengnan Liu, Ming Su, Chunyu Song, Chenyu Lin, Fangying Zhao, Yang Li, Xianyan Zeng, Yong Zhu, Yu Hou, Chunguang Ren, Huan Zhang, Ping Yi, Yong Ji, Chao Wang, Hongtao Li, Ming Ma, Lingfei Luo, Li Li
Coat protein complex I (COPI) vesicles mediate the retrograde transfer of cargo between Golgi cisternae and from the Golgi to the endoplasmic reticulum (ER). However, their roles in the cell cycle and proliferation are unclear. This study shows that TANGO6 associates with COPI vesicles via two transmembrane domains. The TANGO6 N- and C-terminal cytoplasmic fragments capture RNA polymerase II subunit B (RPB) 2 in the cis-Golgi during the G1 phase. COPI-docked TANGO6 carries RPB2 to the ER and then to the nucleus...
March 15, 2024: Nature Communications
https://read.qxmd.com/read/38489271/protocol-to-visualize-and-quantify-the-copii-concentration-and-anterograde-transport-of-nascent-g-protein-coupled-receptors
#6
JOURNAL ARTICLE
Xin Xu, Guangyu Wu
Here, we present a protocol for visualization and quantification of the recruitment of newly synthesized G protein-coupled receptors (GPCRs) to coat protein complex II vesicles and GPCR transport from the endoplasmic reticulum through the Golgi to the cell surface in the retention using the selective hooks assay. We describe steps for plasmid construction, cell transfection, transport synchronization, confocal microscope imaging, and quantification. This protocol is also applicable for studying the transport of non-GPCR cargoes...
March 13, 2024: STAR protocols
https://read.qxmd.com/read/38482914/universal-nanocomposite-coating-with-antifouling-and-redox-capabilities-for-electrochemical-affinity-biosensing-in-complex-biological-fluids
#7
JOURNAL ARTICLE
Aditya Manu Bharti, R K Rakesh Kumar, Cheng-Hsin Chuang, Muhammad Omar Shaikh
Electrochemical affinity biosensors have the potential to facilitate the development of multiplexed point-of-care diagnostics in complex biological fluids. However, their commercial viability has been hindered by challenges such as electrode biofouling and the lack of inherent redox properties. To address this unmet need, we have developed a universal nanocomposite coating which is unique in its ability to not only allow oriented conjugation of the biorecognition element but also specific detection directly in complex biological fluids like serum and urine owing to its built-in antifouling and redox capabilities, thus improving suitability for point of care testing...
March 14, 2024: Nanoscale Horizons: the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy
https://read.qxmd.com/read/38472177/functionalized-graphene-oxide-grids-enable-high-resolution-cryo-em-structures-of-the-snf2h-nucleosome-complex-without-crosslinking
#8
JOURNAL ARTICLE
Un Seng Chio, Eugene Palovcak, Anton A A Smith, Henriette Autzen, Elise N Muñoz, Zanlin Yu, Feng Wang, David A Agard, Jean-Paul Armache, Geeta J Narlikar, Yifan Cheng
Single-particle cryo-EM is widely used to determine enzyme-nucleosome complex structures. However, cryo-EM sample preparation remains challenging and inconsistent due to complex denaturation at the air-water interface (AWI). Here, to address this issue, we develop graphene-oxide-coated EM grids functionalized with either single-stranded DNA (ssDNA) or thiol-poly(acrylic acid-co-styrene) (TAASTY) co-polymer. These grids protect complexes between the chromatin remodeler SNF2h and nucleosomes from the AWI and facilitate collection of high-quality micrographs of intact SNF2h-nucleosome complexes in the absence of crosslinking...
March 12, 2024: Nature Communications
https://read.qxmd.com/read/38458776/novel-peptide-based-oncolytic-vaccine-for-enhancement-of-adaptive-antitumor-immune-response-via-co-engagement-of-innate-fc%C3%AE-and-fc%C3%AE-receptors
#9
JOURNAL ARTICLE
Sara Feola, Firas Hamdan, Salvatore Russo, Jacopo Chiaro, Manlio Fusciello, Michaela Feodoroff, Gabriella Antignani, Federica D'Alessio, Riikka Mölsä, Virpi Stigzelius, Paolo Bottega, Sari Pesonen, Jeanette Leusen, Mikaela Grönholm, Vincenzo Cerullo
BACKGROUND: Cancer immunotherapy relies on using the immune system to recognize and eradicate cancer cells. Adaptive immunity, which consists of mainly antigen-specific cytotoxic T cells, plays a pivotal role in controlling cancer progression. However, innate immunity is a necessary component of the cancer immune response to support an immunomodulatory state, enabling T-cell immunosurveillance. METHODS: Here, we elucidated and exploited innate immune cells to sustain the generation of antigen-specific T cells on the use of our cancer vaccine platform...
March 8, 2024: Journal for Immunotherapy of Cancer
https://read.qxmd.com/read/38436801/point-of-care-dengue-detection-polydopamine-modified-electrode-for-rapid-ns1-protein-testing-for-clinical-samples
#10
JOURNAL ARTICLE
Sjaikhurrizal El Muttaqien, Indra Memdi Khoris, Jodi Suryanggono, Provash C Sadhukhan, Sabar Pambudi, Ankan Dutta Chowdhury, Enoch Y Park
Early diagnosis of dengue infection by detecting the dengue virus non-structural protein 1 (DENV-NS1) is important to the patients to initiate speedy treatment. Enzyme-linked immunosorbent assay (ELISA)-based NS1 detection and RT-PCR are time-consuming and too complex to be employed in remote areas of dengue-endemic countries. Meanwhile, those of NS1 rapid test by lateral flow assay suffer from low detection limit. Electrochemical-based biosensors using screen-printed gold electrodes (SPGEs) have become a reliable detection method to convey both ELISA's high sensitivity and rapid test portability...
March 4, 2024: Mikrochimica Acta
https://read.qxmd.com/read/38382672/charge-neutralization-and-%C3%AE-elimination-cleavage-mechanism-of-family-42-l-rhamnose-%C3%AE-1-4-d-glucuronate-lyase-revealed-using-neutron-crystallography
#11
JOURNAL ARTICLE
Naomine Yano, Tatsuya Kondo, Katsuhiro Kusaka, Takatoshi Arakawa, Tatsuji Sakamoto, Shinya Fushinobu
Gum arabic (GA) is widely used as an emulsion stabilizer and edible coating and consists of a complex carbohydrate moiety with a rhamnosyl-glucuronate group capping the non-reducing ends. Enzymes that can specifically cleave the glycosidic chains of GA and modify their properties are valuable for structural analysis and industrial application. Cryogenic X-ray crystal structure of GA-specific L-rhamnose-α-1,4-D-glucuronate lyase from Fusarium oxysporum (FoRham1), belonging to the polysaccharide lyase (PL) family 42, has been previously reported...
February 19, 2024: Journal of Biological Chemistry
https://read.qxmd.com/read/38378738/biosynthesis-and-biocompatibility-evaluation-of-zinc-oxide-nanoparticles-prepared-using-priestia-megaterium-bacteria
#12
JOURNAL ARTICLE
Mona A Ashour, Basma T Abd-Elhalim
The current study aimed to find an effective, simple, ecological, and nontoxic method for bacterial green synthesis of zinc oxide nanoparticles (ZnONPs) using the bacterial strain Priestia megaterium BASMA 2022 (OP572246). The biosynthesis was confirmed by the change in color of the cell-free supernatant added to the zinc nitrate from yellow to pale brown. The Priestia megaterium zinc oxide nanoparticles (Pm/ZnONPs) were characterized using UV-Vis spectroscopy, high-resolution transmission electron microscopy (HR-TEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR), and zeta potential...
February 20, 2024: Scientific Reports
https://read.qxmd.com/read/38369384/role-of-aminopeptidase-n-like-in-the-acquisition-of-begomoviruses-by-bemisia-tabaci-the-whitefly-vector
#13
JOURNAL ARTICLE
Yun-Yun Fan, Yao Chi, Na Chen, Wilmer J Cuellar, Xiao-Wei Wang
Sri Lankan cassava mosaic virus (SLCMV) is a prominent causative agent of cassava mosaic disease in Asia and relies on the whitefly Bemisia tabaci cryptic complex for its transmission. However, the molecular mechanisms involved in SLCMV transmission by B. tabaci have yet to be understood. In this study, we identified an aminopeptidase N-like protein (BtAPN) in B. tabaci Asia II 1, an efficient vector of SLCMV, which is involved in the SLCMV transmission process. Through the use of glutathione S-transferase pull-down assay and LC-MS/MS analysis, we demonstrated the interaction between BtAPN and the coat protein (CP) of SLCMV...
February 18, 2024: Insect Science
https://read.qxmd.com/read/38362621/mucin-coated-protein-polyphenol-microcarriers-for-daidzein-delivery
#14
JOURNAL ARTICLE
Su Hui Lim, Germaine Jia Min Yong, Cheryl Yingxue Chia, Shu Mei Man, Gomathy Sandhya Subramanian, Geraldine Oh, Eleanor Jing Yi Cheong, Maxim V Kiryukhin
Daidzein, an isoflavone found abundantly in legumes, may benefit from bypassing upper gut absorption to reach the colon where it can be metabolized into the potent estrogen equol by the gut microbiome. To achieve this, we developed mucin coated protein-tannin multilayer microcarriers. Highly porous functionalized calcium carbonate (FCC) microparticles efficiently absorbed daidzein from a dimethyl sulfoxide solution, with a loading capacity of 21.6 ± 1.8 wt% as measured by ultra-high pressure liquid chromatography - mass spectrometry (UPLC-MS)...
February 16, 2024: Food & Function
https://read.qxmd.com/read/38339916/dual-protection-inorganic-protein-coating-on-mg-based-biomaterials-through-tooth-enamel-inspired-biomineralization
#15
JOURNAL ARTICLE
Ziqian Zhao, Wenting Yu, Wenshuai Yang, Guohao Zhang, Charley Huang, Jianmin Han, Ravin Narain, Hongbo Zeng
Biocompatible magnesium alloys represent revolutionary implantable materials in dentistry and orthopedics but face challenges due to rapid bio-corrosion, necessitating protective coatings to mitigate dysfunction. Directly integrating durable protective coatings onto Mg surfaces is challenging because of intrinsic low coating compactness. Herein, inspired by tooth enamel, a novel highly compact dual-protection inorganic-protein (inorganicPro) coating is in-situ constructed on Mg surfaces through bovine serum albumin (BSA) protein-boosted reaction between sodium fluoride (NaF) and Mg substrates...
February 10, 2024: Advanced Materials
https://read.qxmd.com/read/38336007/human-serum-albumin-in-the-presence-of-small-platinum-nanoparticles
#16
JOURNAL ARTICLE
Xiaomin Yang, Erika Porcel, Laurent Marichal, Cesar Gonzalez Vargas, Amine Khitous, Daniela Salado-Leza, Xue Li, Jean-Philippe Renault, Serge Pin, Hynd Remita, Frank Wien, Sandrine Lacombe
Noble metal materials, especially platinum nanoparticles (Pt NPs), have immense potential in nanomedicine as therapeutic agents on account of their high electron density and their high surface area. Intravenous injection is proposed as the best mode to deliver the product to patients. However, our understanding of the reaction of nanoparticles with blood components, especially proteins, is far behind the explosive development of these agents. Using synchrotron radiation circular dichroism (SRCD), we investigated the structural and stability changes of human serum albumin (HSA) upon interaction with PEG-OH coated Pt NPs at nanomolar concentrations, conditions potentially encountered for intravenous injection...
February 7, 2024: Journal of Pharmaceutical Sciences
https://read.qxmd.com/read/38334330/lymphocytic-choriomeningitis-arenavirus-requires-cellular-copi-and-ap-4-complexes-for-efficient-virion-production
#17
JOURNAL ARTICLE
Owen Byford, Amelia B Shaw, Hiu Nam Tse, Eleanor J A A Todd, Beatriz Álvarez-Rodríguez, Roger Hewson, Juan Fontana, John N Barr
Lymphocytic choriomeningitis virus (LCMV) is a bisegmented negative-sense RNA virus classified within the Arenaviridae family of the Bunyavirales order. LCMV is associated with fatal disease in immunocompromized populations, and as the prototypical arenavirus, acts as a model for the many serious human pathogens within this group. Here, we examined the dependence of LCMV multiplication on cellular trafficking components using a recombinant LCMV expressing enhanced green fluorescent protein in conjunction with a curated siRNA library...
February 9, 2024: Journal of Virology
https://read.qxmd.com/read/38307351/arabidopsis-hapless13-ap-1%C3%A2%C2%B5-is-critical-for-pollen-sac-formation-and-tapetal-function
#18
JOURNAL ARTICLE
Gui-Min Yin, Yi-Ru Fang, Jia-Gang Wang, Yue Liu, Xiaojiao Xiang, Sha Li, Yan Zhang
The production of excess and viable pollen grains is critical for reproductive success of flowering plants. Pollen grains are produced within anthers, the male reproductive organ whose development involves precisely controlled cell differentiation, division, and intercellular communication. In Arabidopsis thaliana, specification of an archesporial cell (AC) at four corners of a developing anther, followed by programmed cell divisions, generates four pollen sacs, walled by four cell layers among which the tapetum is in close contact with developing microspores...
January 31, 2024: Plant Science: An International Journal of Experimental Plant Biology
https://read.qxmd.com/read/38294348/development-of-an-online-isotope-dilution-ce-icp-ms-method-for-the-quantification-of-sulfur-in-biological-compounds
#19
JOURNAL ARTICLE
Dariya Tukhmetova, Jan Lisec, Jochen Vogl, Björn Meermann
We report an analytical methodology for the quantification of sulfur in biological molecules via a species-unspecific postcolumn isotope dilution (online ID) approach using capillary electrophoresis (CE) coupled online with inductively coupled plasma-mass spectrometry (online ID CE/ICP-MS). The method was optimized using a mixture of standard compounds including sulfate, methionine, cysteine, cystine, and albumin, yielding compound recoveries between 98 and 105%. The quantity of sulfur is further converted to the quantity of the compounds owing to the prior knowledge of the sulfur content in the molecules...
January 31, 2024: Analytical Chemistry
https://read.qxmd.com/read/38289472/a-novel-aminopeptidase-n-cd13-inhibitor-selectively-targets-an-endothelial-form-of-cd13-after-coupling-to-proteins
#20
JOURNAL ARTICLE
Giulia Anderluzzi, Michela Ghitti, Anna Maria Gasparri, Giulia Taiè, Angelina Sacchi, Alessandro Gori, Annapaola Andolfo, Federica Pozzi, Giovanna Musco, Flavio Curnis, Angelo Corti
Aminopeptidase N/CD13, a membrane-bound enzyme upregulated in tumor vasculature and involved in angiogenesis, can be used as a receptor for the targeted delivery of drugs to tumors through ligand-directed targeting approaches. We describe a novel peptide ligand (VGCARRYCS, called "G4") that recognizes CD13 with high affinity and selectivity. Enzymological and computational studies showed that G4 is a competitive inhibitor that binds to the catalytic pocket of CD13 through its N-terminal region. Fusing the peptide C-terminus to tumor necrosis factor-alpha (TNF) or coupling it to a biotin/avidin complex causes loss of binding and inhibitory activity against different forms of CD13, including natural or recombinant ectoenzyme and a membrane form expressed by HL60 promyelocytic leukemia cells (likely due to steric hindrance), but not binding to a membrane form of CD13 expressed by endothelial cells (ECs)...
January 30, 2024: Cellular and Molecular Life Sciences: CMLS
keyword
keyword
160604
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.