Chen Wang, Dongliang Xiao, Baoqing Dun, Miaomiao Yin, Adigo Setargie Tsega, Linan Xie, Wenhua Li, Qun Yue, Sibao Wang, Han Gao, Min Lin, Liwen Zhang, István Molnár, Yuquan Xu
Xylomyrocins, a unique group of nonribosomal peptide secondary metabolites, were discovered in Paramyrothecium and Colletotrichum spp. fungi by employing a combination of high-resolution tandem mass spectrometry (HRMS/MS)-based chemometrics, comparative genome mining, gene disruption, stable isotope feeding, and chemical complementation techniques. These polyol cyclodepsipeptides all feature an unprecedented d-xylonic acid moiety as part of their macrocyclic scaffold. This biosynthon is derived from d-xylose supplied by xylooligosaccharide catabolic enzymes encoded in the xylomyrocin biosynthetic gene cluster, revealing a novel link between carbohydrate catabolism and nonribosomal peptide biosynthesis...
August 9, 2022: Proceedings of the National Academy of Sciences of the United States of America