Vidya Padmanabhan Nair, Hengyuan Liu, Gabriele Ciceri, Johannes Jungverdorben, Goar Frishman, Jason Tchieu, Gustav Y Cederquist, Ina Rothenaigner, Kenji Schorpp, Lena Klepper, Ryan M Walsh, Tae Wan Kim, Daniela Cornacchia, Andreas Ruepp, Jens Mayer, Kamyar Hadian, Dmitrij Frishman, Lorenz Studer, Michelle Vincendeau
The biological function and disease association of human endogenous retroviruses (HERVs) are largely elusive. HERV-K(HML-2) has been associated with neurotoxicity, but there is no clear understanding of its role or mechanistic basis. We addressed the physiological functions of HERV-K(HML-2) in neuronal differentiation using CRISPR engineering to activate or repress its expression levels in a human-pluripotent-stem-cell-based system. We found that elevated HERV-K(HML-2) transcription is detrimental for the development and function of cortical neurons...
April 26, 2021: Cell Stem Cell