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• keyword#1
Honi Sanders, Daoyun Ji, Takuya Sasaki, Jill K Leutgeb, Matthew A Wilson, John E Lisman
Hippocampal place cells represent non-spatial information in addition to their more frequently studied spatial response properties. This encoding of non-spatial information by a process called rate remapping involves the increase or decrease in the firing rate of a place cell without changes in its spatial specificity. However, many hippocampal phenomena occur on very short time scales over which long-term average firing rates are not an appropriate description of activity. To understand how rate remapping relates to fine-scale temporal firing phenomena in the hippocampus, we studied place cell responses during two tasks that induce rate remapping...
August 21, 2018: Hippocampus
#2
Said Hafidh, David Potěšil, Karel Müller, Jan Fíla, Christos Michailidis, Anna Herrmannová, Jana Feciková, Till Ischebeck, Leoš Shivaya Valášek, Zbyněk Zdráhal, David Honys
Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are utilized later for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco ( Nicotiana tabacum ), long-term storage EDTA/puromycin-resistant particles, translating polysomes, and free ribonuclear particles, throughout tobacco pollen development and in in vitro-growing pollen tubes...
September 2018: Plant Physiology
#3
Ladislav Dokládal, Eva Benková, David Honys, Nikoleta Dupľáková, Lan-Ying Lee, Stanton B Gelvin, Eva Sýkorová
Arabidopsis and human ARM protein interact with telomerase. Deregulated mRNA levels of DNA repair and ribosomal protein genes in an Arabidopsis arm mutant suggest non-telomeric ARM function. The human homolog ARMC6 interacts with hTRF2. Telomerase maintains telomeres and has proposed non-telomeric functions. We previously identified interaction of the C-terminal domain of Arabidopsis telomerase reverse transcriptase (AtTERT) with an armadillo/β-catenin-like repeat (ARM) containing protein. Here we explore protein-protein interactions of the ARM protein, AtTERT domains, POT1a, TRF-like family and SMH family proteins, and the chromatin remodeling protein CHR19 using bimolecular fluorescence complementation (BiFC), yeast two-hybrid (Y2H) analysis, and co-immunoprecipitation...
July 2018: Plant Molecular Biology
#4
Jana Majerská, Petra Procházková Schrumpfová, Ladislav Dokládal, Šárka Schořová, Karel Stejskal, Michal Obořil, David Honys, Lucie Kozáková, Pavla Sováková Polanská, Eva Sýkorová
In the published online version, the affiliations were mixed up. Corrected affiliation section is shown below. Also, the update has also been reflected in the author group section above.
March 2018: Protoplasma
#5
Lenka Záveská Drábková, David Honys
Callose is a plant-specific polysaccharide (β-1,3-glucan) playing an important role in angiosperms in many developmental processes and responses to biotic and abiotic stresses. Callose is synthesised at the plasma membrane of plant cells by callose synthase (CalS) and, among others, represents the main polysaccharide in the callose wall surrounding the tetrads of developing microspores and in the growing pollen tube wall. CalS proteins involvement in spore development is a plesiomorphic feature of terrestrial plants, but very little is known about their evolutionary origin and relationships amongst the members of this protein family...
2017: PloS One
#6
Jan Fíla, David Honys
Protein phosphorylation was repeatedly shown to be the most dynamic post-translational modification mediated by a huge orchestra of protein kinases and phosphatases. Upon landing on a stigma, pollen grain dehydration and activation are accompanied by changes in protein phosphorylation together with the translation activation of stored mRNAs. To enable studies of the total phosphoproteome, it is usually necessary to apply various enrichment techniques. In this chapter, one of these protocols that worked previously well on tobacco mature pollen is presented in more detail...
2017: Methods in Molecular Biology
#7
Komathi Kolandai-Matchett, Erika Langham, Maria Bellringer, Pesio Ah-Honi Siitia
Pacific people in New Zealand are a minority ethnic population identified in national prevalence studies as having the highest risk of developing gambling problems. As earlier studies identified some links between culture and gambling for this population, our study aimed to deepen understanding of these links and their role in explaining the disproportionate gambling harms experienced by Pacific people. To achieve this aim we employed intersectionality as a theoretical framework to explore the culture-gambling intersection for this population group...
2017: Asian Journal of Gambling Issues and Public Health
#8
Cecilia Y Taing, Alice A Gibson, Stephen Colagiuri, Philip Vita, Magnolia Cardona-Morrell, Adrian Bauman, Michael Moore, Mandy Williams, Andrew Milat, Jacky Hony, Sophia Lin, Melissa Gwizd, Maria A Fiatarone Singh
AIM: There is strong and consistent evidence from large scale randomised controlled trials that type 2 diabetes can be prevented or delayed through lifestyle modification which improves diet quality, increases physical activity and achieves weight loss in people at risk. Worldwide, the prevalence of type 2 diabetes is increasing in individuals of Chinese descent. Culturally tailored programs are required to address the risk in the Chinese population. This paper analyses effectiveness of a culturally tailored community-based lifestyle modification program (Sydney Diabetes Prevention Program (SDPP)) targeting Mandarin speakers...
October 2017: Diabetes Research and Clinical Practice
#9
Md Shahidul Islam, Jennifer A Saito, Emdadul Mannan Emdad, Borhan Ahmed, Mohammad Moinul Islam, Abdul Halim, Quazi Md Mosaddeque Hossen, Md Zakir Hossain, Rasel Ahmed, Md Sabbir Hossain, Shah Md Tamim Kabir, Md Sarwar Alam Khan, Md Mursalin Khan, Rajnee Hasan, Nasima Aktar, Ummay Honi, Rahin Islam, Md Mamunur Rashid, Xuehua Wan, Shaobin Hou, Taslima Haque, Muhammad Shafiul Azam, Mahdi Muhammad Moosa, Sabrina M Elias, A M Mahedi Hasan, Niaz Mahmood, Md Shafiuddin, Saima Shahid, Nusrat Sharmeen Shommu, Sharmin Jahan, Saroj Roy, Amlan Chowdhury, Ashikul Islam Akhand, Golam Morshad Nisho, Khaled Salah Uddin, Taposhi Rabeya, S M Ekramul Hoque, Afsana Rahman Snigdha, Sarowar Mortoza, Syed Abdul Matin, Md Kamrul Islam, M Z H Lashkar, Mahboob Zaman, Anton Yuryev, Md Kamal Uddin, Md Sharifur Rahman, Md Samiul Haque, Md Monjurul Alam, Haseena Khan, Maqsudul Alam
Jute (Corchorus sp.) is one of the most important sources of natural fibre, covering ∼80% of global bast fibre production1 . Only Corchorus olitorius and Corchorus capsularis are commercially cultivated, though there are more than 100 Corchorus species2 in the Malvaceae family. Here we describe high-quality draft genomes of these two species and their comparisons at the functional genomics level to support tailor-designed breeding. The assemblies cover 91.6% and 82.2% of the estimated genome sizes for C. olitorius and C...
January 30, 2017: Nature Plants
#10
Jana Fišerová Jurečková, Eva Sýkorová, Said Hafidh, David Honys, Jiří Fajkus, Miloslava Fojtová
In tobacco, three sequence variants of the TERT gene have been described. We revealed unbalanced levels of TERT variant transcripts in vegetative tobacco tissues and enhanced TERT transcription and telomerase activity in reproductive tissues. Telomerase is a ribonucleoprotein complex responsible for the maintenance of telomeres, structures delimiting ends of linear eukaryotic chromosomes. In the Nicotiana tabacum (tobacco) allotetraploid plant, three sequence variants (paralogs) of the gene coding for the telomerase reverse transcriptase subunit (TERT) have been described, two of them derived from the maternal N...
March 2017: Planta
#11
Antónia Gibalová, Lenka Steinbachová, Said Hafidh, Veronika Bláhová, Zuzana Gadiou, Christos Michailidis, Karel Műller, Roman Pleskot, Nikoleta Dupľáková, David Honys
KEY MESSAGE : bZIP TF network in pollen. Transcriptional control of gene expression represents an important mechanism guiding organisms through developmental processes and providing plasticity towards environmental stimuli. Because of their sessile nature, plants require effective gene regulation for rapid response to variation in environmental and developmental conditions. Transcription factors (TFs) provide such control ensuring correct gene expression in spatial and temporal manner. Our work reports the interaction network of six bZIP TFs expressed in Arabidopsis thaliana pollen and highlights the potential functional role for AtbZIP18 in pollen...
March 2017: Plant Reproduction
#12
Jana Majerská, Petra Procházková Schrumpfová, Ladislav Dokládal, Šárka Schořová, Karel Stejskal, Michal Obořil, David Honys, Lucie Kozáková, Pavla Sováková Polanská, Eva Sýkorová
The life cycle of telomerase involves dynamic and complex interactions between proteins within multiple macromolecular networks. Elucidation of these associations is a key to understanding the regulation of telomerase under diverse physiological and pathological conditions from telomerase biogenesis, through telomere recruitment and elongation, to its non-canonical activities outside of telomeres. We used tandem affinity purification coupled to mass spectrometry to build an interactome of the telomerase catalytic subunit AtTERT, using Arabidopsis thaliana suspension cultures...
July 2017: Protoplasma
#13
Nikoleta Dupl'áková, Petre I Dobrev, David Reňák, David Honys
Research investigating the dynamics of male gametophyte (MG) development has proven to be challenging for the plant science community. Here we describe our protocol for separating Arabidopsis MG developmental stages, which is based on the centrifugation of pollen through a discontinuous Percoll concentration gradient. This Percoll gradient can be formed using a pipette, and it does not require a gradient maker. The purity of the isolated developing spores is as high as 70%, and in most separations it is well above 80%...
October 2016: Nature Protocols
#14
Said Hafidh, David Potěšil, Jan Fíla, Věra Čapková, Zbyněk Zdráhal, David Honys
BACKGROUND: As in animals, cell-cell communication plays a pivotal role in male-female recognition during plant sexual reproduction. Prelaid peptides secreted from the female reproductive tissues guide pollen tubes towards ovules for fertilization. However, the elaborate mechanisms for this dialogue have remained elusive, particularly from the male perspective. RESULTS: We performed genome-wide quantitative liquid chromatography-tandem mass spectrometry analysis of a pistil-stimulated pollen tube secretome and identified 801 pollen tube-secreted proteins...
May 3, 2016: Genome Biology
#15
Jan Fíla, Sonja Radau, Andrea Matros, Anja Hartmann, Uwe Scholz, Jana Feciková, Hans-Peter Mock, Věra Čapková, René Peiman Zahedi, David Honys
Tobacco mature pollen has extremely desiccated cytoplasm, and is metabolically quiescent. Upon re-hydration it becomes metabolically active and that results in later emergence of rapidly growing pollen tube. These changes in cytoplasm hydration and metabolic activity are accompanied by protein phosphorylation. In this study, we subjected mature pollen, 5-min-activated pollen, and 30-min-activated pollen to TCA/acetone protein extraction, trypsin digestion and phosphopeptide enrichment by titanium dioxide. The enriched fraction was subjected to nLC-MS/MS...
April 2016: Molecular & Cellular Proteomics: MCP
#16
REVIEW
Said Hafidh, Jan Fíla, David Honys
Overview of pollen development. Male gametophyte development of angiosperms is a complex process that requires coordinated activity of different cell types and tissues of both gametophytic and sporophytic origin and the appropriate specific gene expression. Pollen ontogeny is also an excellent model for the dissection of cellular networks that control cell growth, polarity, cellular differentiation and cell signaling. This article describes two sequential phases of angiosperm pollen ontogenesis-developmental phase leading to the formation of mature pollen grains, and a functional or progamic phase, beginning with the impact of the grains on the stigma surface and ending at double fertilization...
June 2016: Plant Reproduction
#17
Ladislav Dokládal, David Honys, Rajiv Rana, Lan-Ying Lee, Stanton B Gelvin, Eva Sýkorová
Telomerase-reverse transcriptase (TERT) plays an essential catalytic role in maintaining telomeres. However, in animal systems telomerase plays additional non-telomeric functional roles. We previously screened an Arabidopsis cDNA library for proteins that interact with the C-terminal extension (CTE) TERT domain and identified a nuclear-localized protein that contains an RNA recognition motif (RRM). This RRM-protein forms homodimers in both plants and yeast. Mutation of the gene encoding the RRM-protein had no detectable effect on plant growth and development, nor did it affect telomerase activity or telomere length in vivo, suggesting a non-telomeric role for TERT/RRM-protein complexes...
2015: Frontiers in Plant Science
#18
REVIEW
Honi Sanders, César Rennó-Costa, Marco Idiart, John Lisman
Much has been learned about the hippocampal/entorhinal system, but an overview of how its parts work in an integrated way is lacking. One question regards the function of entorhinal grid cells. We propose here that their fundamental function is to provide a coordinate system for producing mind-travel in the hippocampus, a process that accesses associations with upcoming positions. We further propose that mind-travel occurs during the second half of each theta cycle. By contrast, the first half of each theta cycle is devoted to computing current position using sensory information from the lateral entorhinal cortex (LEC) and path integration information from the medial entorhinal cortex (MEC)...
December 2015: Trends in Neurosciences
#19
Rebecca Thompson, Honie Thomson, Virginia Plummer
No abstract text is available yet for this article.
March 2015: Australian Nursing & Midwifery Journal
#20
Pavel Bokvaj, Said Hafidh, David Honys
Pollen, an extremely reduced bicellular or tricellular male reproductive structure of flowering plants, serves as a model for numerous studies covering wide range of developmental and physiological processes. The pollen development represents a fragile and vital phase of plant ontogenesis and pollen was among the first singular plant tissues thoroughly characterized at the transcriptomic level (Honys and Twell [5]). Arabidopsis pollen developmental transcriptome has been published over a decade ago (Honys and Twell, 2004) and transcriptomes of developing pollen of other species have followed (Rice, Deveshwar et al...
March 2015: Genomics Data
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