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fibrinogen degradation

Rui-Qing Hou, Nico Scharnagl, Regine Willumeit-Römer, Frank Feyerabend
Bovine serum albumin (BSA) or fetal bovine serum (FBS), as the protein component, is usually added into solution to study the influence of proteins on Mg degradation. However, the specific character of proteins used and the interaction between organic molecules in FBS do not draw enough attention. This study investigated the influence of BSA, fibrinogen (Fib) and FBS on Mg degradation in Hanks' balanced salt solution without (HBSS) or with calcium (HBSSCa) and Dulbecco's modified eagle medium Glutamax-I (DMEM)...
February 13, 2019: Acta Biomaterialia
Chengguang Yang, Leiming Zhu
BACKGROUND AND PURPOSE: Venous thromboembolism (VTE) is a serious complication encountered in surgical practice. The purpose of this study was to identify changes in coagulation status and deep vein flow parameters, within 24 h postoperatively, for patients undergoing laparoscopic total extraperitoneal inguinal hernia repair (TEP). METHODS: For 144 patients undergoing TEP, coagulation markers including prothrombin time (PT), partial thromboplastin time, thrombin time, D-dimer, fibrinogen, fibrin degradation products (FDP), and international normalized ratio (INR) were monitored preoperatively and in the first morning postoperatively...
February 11, 2019: Surgical Endoscopy
Weiping Cao, Xinzhi Wang, Tingmei Chen, Mingmei Qin, Zuxian Wang, Qin Wang, Bing Xie, Wenlin Xu
RATIONALE: Eclampsia is a life-threatening complication of pregnancy. Eclampsia is a leading cause of maternal and neonatal morbidity and mortality with most injury being associated with the seizures that mark the onset of the disease. It is vital that medical worker managing eclampsia have an understanding of the disease process. PATIENT CONCERNS: A 28-year-old female, G4P1, with history of caesarean section was admitted at GA34+6, in addition to headache and severe hypertension (180/120 mm Hg) and proteinuria (+++)...
February 2019: Medicine (Baltimore)
Nikola Gligorijević, Simeon Minić, Dragana Robajac, Milan Nikolić, Tanja Ćirković Veličković, Olgica Nedić
Fibrinogen, a protein involved in blood coagulation, is very susceptible to oxidation. Oxidation alters its function and usually makes it more thrombogenic. Bilirubin, an end-product of the haem degradation in vertebrates, is known for its antioxidant properties. The present paper describes interaction between fibrinogen and bilirubin, and the influence of bilirubin on the formation of fibrin and protection against oxidation. The binding constant of 4.5 × 104  M-1 was determined for the fibrinogen/bilirubin complex at 37 °C...
January 23, 2019: International Journal of Biological Macromolecules
Vo Van Giau, Seong Soo A An
The vast majority of patients with Alzheimer's disease (AD) suffer from impaired cerebral circulation. Substantial evidence indicates that fibrinogen (Fbg) and fibrin clot formation play an important role in this circulatory dysfunction in AD. Fbg interacts with β-amyloid (1-42) (Aβ), forming plasmin-resistant abnormal blood clots, and increased fibrin deposition has been discovered in the brains of AD patients and mouse models. In this study, biochemical approaches and the epitope mapping immunoassay were employed to characterize binding epitopes within the Fbg and complementary epitopes in Aβ...
January 24, 2019: International Journal of Molecular Sciences
Hongbing Xu, Tong Wang, Shengcong Liu, Robert D Brook, Baihuan Feng, Qian Zhao, Xiaoming Song, Tieci Yi, Jie Chen, Yi Zhang, Yang Wang, Lemin Zheng, Sanjay Rajagopalan, Jianping Li, Wei Huang
RATIONALE: The pathophysiologic mechanisms of air pollution associated exacerbation of cardiovascular events remain incompletely understood. OBJECTIVE: To assess whether ambient air pollution can be a trigger of the vulnerable plaque and heightened thrombogenicity through systemic inflammatory pathways. METHODS AND RESULTS: In Beijing AIRCHD study, seventy-three healthy adults (mean {plus minus} standard deviation, 23.3 {plus minus} 5.4 years) were followed up in 2014-2016...
January 21, 2019: Circulation Research
Monireh Kouhi, Venugopal Jayarama Reddy, Mohammadhossein Fathi, Morteza Shamanian, Afsaneh Valipouri, Seeram Ramakrishna
Guided bone regeneration (GBR) has been established to be an effective method for the repair of defective tissues, which is based on isolating bone defects with a barrier membrane for faster tissue reconstruction. The aim of the present study is to develop poly (hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/fibrinogen (FG)/bredigite (BR) membranes with applicability in GBR. BR nanoparticles were synthesized through a sol-gel method and characterized using transmission electron microscopy (TEM) and X-ray diffractometer (XRD)...
January 12, 2019: Journal of Biomedical Materials Research. Part A
Shuwen Li, Jiakai Lu, Weiping Cheng, Junming Zhu, Mu Jin
PURPOSE: Platelets are crucial components of the coagulation processes, and low admission platelet count (PLC) is associated with adverse clinical outcomes in patients with Stanford type A acute aortic dissection (AAD). METHODS: A total of 130 consecutive patients undergoing Stanford type A AAD surgery in Beijing Anzhen Hospital were enrolled between January 2013 and July 2014. Preoperative clinical and laboratory data from patients were collected. Multiple regression analyses were used to determine the independent factors of low admission platelets...
December 18, 2018: Annals of Thoracic and Cardiovascular Surgery
Ângela Alice Amadeu Megale, Fábio Carlos Magnoli, Alexandre Kazuo Kuniyoshi, Leo Kei Iwai, Denise V Tambourgi, Fernanda C V Portaro, Wilmar Dias da Silva
Background: Bitis arietans is a venomous snake found in sub-Saharan Africa and in parts of Morocco and Saudi Arabia. The envenomation is characterized by local and systemic reactions including pain, blistering, edema and tissue damage, besides hemostatic and cardiovascular disturbances, which can cause death or permanent disabilities in its victims. However, the action mechanisms that provoke these effects remain poorly understood, especially the activities of purified venom components...
2018: Journal of Venomous Animals and Toxins Including Tropical Diseases
T Lipitsä, H Siiskonen, A Naukkarinen, I T Harvima
BACKGROUND: The accumulation of immunoreactants and fibrinoid necrosis of postcapillary vessel walls are common pathological features of cutaneous immune complex vasculitis. In more advanced lesions, these immunoreactants are subject to proteolysis. Mast cell chymase is a powerful enzyme that can degrade several substrates including the extracellular matrix. Heparin can influence the catalytic properties of chymase. OBJECTIVES: We aimed to study the effects of recombinant human (rh) chymase on fibrinogen, coagulation and fibrinolysis, and to relate these effects to the vasculitis pathogenesis...
December 18, 2018: British Journal of Dermatology
Katsuyoshi Ando, Mikihiro Fujiya, Yoshiki Nomura, Yuhei Inaba, Yuya Sugiyama, Yu Kobayashi, Takuya Iwama, Masami Ijiri, Keitaro Takahashi, Nobuhiro Ueno, Shin Kashima, Kentaro Moriichi, Hiroki Tanabe, Yusuke Mizukami, Kazumi Akasaka, Satoshi Fujii, Satoshi Yamada, Hiroshi Nakase, Toshikatsu Okumura
BACKGROUND: Venous thromboembolism (VTE) has been shown to be more frequent in inflammatory bowel disease (IBD) than in the general population in Western studies. However, the actual state of VTE in Asian IBD remains poorly understood. AIMS: To reveal the incidence of VTE in IBD patients in Japan. METHODS: Eighty-five patients admitted to 3 gastroenterology centers were registered from 2013 to 2018. The incidence of VTE in patients with IBD (n = 42) was prospectively compared to that among patients with other digestive diseases (n = 43)...
December 14, 2018: Digestion
Liya Jiang, Qiaohong Zhang, Wanping Xu, Yongjun Zhang
OBJECTIVE: To study the clinical phenotype and gene mutation analysis of a hereditary abnormal fibrinogenemia family and explore its molecular pathogenesis. METHODS: The STA-R automatic hemagglutination analyzer to detect the proband and its family members (3 generations of 5 people) of prothrombin time(PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen activity (Fg: C), D-dimer (D-D), fibrinogen and fibrin degradation products (FDPs), plasminogen activity (PLG: A); The plasma levels of Fg: C and fibrinogen (Fg: Ag) were measured by Clauss method and immunoturbidimetry respectively...
December 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Fernanda G Amorim, Danilo L Menaldo, Sante E I Carone, Thiago A Silva, Marco A Sartim, Edwin De Pauw, Loic Quinton, Suely V Sampaio
Snake venom serine proteases (SVSPs) are enzymes that are capable of interfering in various parts of the blood coagulation cascade, which makes them interesting candidates for the development of new therapeutic drugs. Herein, we isolated and characterized Moojase, a potent coagulant enzyme from Bothrops moojeni snake venom. The toxin was isolated from the crude venom using a two-step chromatographic procedure. Moojase is a glycoprotein with N-linked glycans, molecular mass of 30.3 kDa and acidic character (pI 5...
November 28, 2018: Toxins
Yang Fu, Yumei Liu, Si Chen, Yaxiong Jin, Hong Jiang
To evaluate the correlation between the Caprini risk assessment scale and plasma thrombosis biomarkers and estimate the validity of this method in identifying critically ill patients at high risk of venous thromboembolism (VTE).Patients with VTE who were admitted to the intensive care unit (ICU) department of West China Hospital SiChuan University from October 2016 to October 2017 were enrolled in this case-control study. We retrieved relative clinical data and laboratory test results included in the Caprini risk assessment scale to calculate the Caprini score and compared thrombosis biomarkers between various risk stratifications (low, moderate, high, and highest)...
November 2018: Medicine (Baltimore)
Ruiqing Hou, Regine Willumeit-Römer, Vasil M Garamus, Marion Frant, Joachim Koll, Frank Feyerabend
Although the adsorption of proteins on Mg surface was ascribed to be the main reason for the effect of proteins on magnesium (Mg) degradation, few studies about the adsorption of proteins on Mg surface were performed due to the labile circumstance during immersion. In the present study, the adsorption of bovine serum albumin (BSA) and fibrinogen (Fib) on Mg surface during and after immersion was extensively investigated in different media for the first time. The results revealed that BSA and Fib showed a similar adsorption trend on Mg surface during and after immersion, and they adsorbed more on Mg surface in HBSS than in DMEM...
November 15, 2018: ACS Applied Materials & Interfaces
Miyuki Ookura, Naoko Hosono, Toshiki Tasaki, Kana Oiwa, Kei Fujita, Kazuhiro Ito, Shin Lee, Yasufumi Matsuda, Mihoko Morita, Katsunori Tai, Eiju Negoro, Shinji Kishi, Hiromichi Iwasaki, Takanori Ueda, Takahiro Yamauchi
Disseminated intravascular coagulation (DIC) is a life-threatening condition that frequently occurs in patients with hematologic malignancies. Currently, recombinant human soluble thrombomodulin (rTM) is a therapeutic DIC drug that is manufactured and sold in Japan only. We evaluated the efficacy of rTM compared to that of gabexate mesilate (GM), which was previously used routinely for treating DIC in Japan, in patients with acute myeloid leukemia (AML). This retrospective study enrolled 43 AML patients, including 17 with acute promyelocytic leukemia (APL), that was complicated with DIC...
November 2018: Medicine (Baltimore)
Richard W Lo, Ling Li, Richard Leung, Fred G Pluthero, Walter H A Kahr
Objective- Human and mouse megakaryocytes lacking NBEAL2 (neurobeachin-like 2) produce platelets where α-granules lack protein cargo. This cargo is mostly megakaryocyte-synthesized, but some proteins, including FGN (fibrinogen), are endocytosed. In this study, we examined the trafficking of both types of cargo within primary megakaryocytes cultured from normal and NBEAL2-null mice, to determine the role of NBEAL2 in α-granule maturation. We also examined the interaction of NBEAL2 with the granule-associated protein P-selectin in human megakaryocytes and platelets...
October 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
C J Li, Z H Yang, F G Lu, X L Shi, D L Liu
BACKGROUND AND STUDY AIMS: To investigate the relationship among fibrotic, haemostatic and endotoxic changes in patients with different degrees of liver cirrhosis. PATIENTS AND METHODS: Liver fibrotic markers, including hyaluronic acid (HA), Ccollagen IV (Col-IV), laminin (LN), and N-terminal pro-peptide of collagen type III (PIIINP), were determined by radioimmunoassay. A series of haemostatic tests, including prothrombin time (PT), international normalized ratio, activated partial thromboplastin time, antithrombin-III, thrombin time, fibrinogen, fibrin(ogen) degradation product and D-dimer were determined using an automatic coagulation analyszer...
July 2018: Acta Gastro-enterologica Belgica
Pasquale Picone, Maria Antonietta Sabatino, Alessia Ajovalasit, Daniela Giacomazza, Clelia Dispenza, Marta Di Carlo
Crosslinked xyloglucan-poly(vinyl alcohol) based hydrogel films are interesting materials for wound healing applications. This work focuses on the hydrolytic degradation and consequent morphological modification of a XG-PVA film and on its interaction with cells, blood, bacteria. Biocompatibility of the film was assessed in vitro by investigating different aspects, such as cell viability, oxidative stress level, mitochondrial dysfunction and specific stress biomarkers. Partial adhesiveness was demonstrated by performing different attaching assays and phalloidin staining...
January 2019: International Journal of Biological Macromolecules
Jing-Ye Wang, Gao-Jia Zhang, Sheng-Xia Zhuo, Kai Wang, Xiao-Peng Hu, Hui Zhang, Lin-di Qu
BACKGROUND: The patterns and mechanisms underlying stroke in cancer patients differ from those of the conventional etiology. In this study, we further investigated the characteristics distinguishing cancer-associated ischemic stroke (CAIS) and the relationship of D-dimer value with CAIS. METHODS: Sixty-one acute ischemic stroke patients with cancer (cancer group) and 76 stroke patients without cancer (control group) were recruited. Cerebrovascular distribution was divided into 3 circulations and 23 vascular territories, and acute multiple brain infarcts (AMBIs) were defined as discrete MRI diffusion-weighted imaging (DWI) lesions in >1 vascular territory...
November 2018: Neurological Research
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