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fibrinogen denature

Daiki Murakami, Nami Mawatari, Toshiki Sonoda, Aki Kashiwazaki, Masaru Tanaka
The blood-compatible polymer poly(2-methoxyethyl acrylate) (PMEA) is composed of nanometer-scale interfacial structures because of the phase separation of the polymer and water at the PMEA/phosphate-buffered saline (PBS) interface. We synthesized PMEA with four different molecular weights (19, 30, 44, 183 kg/mol) to investigate the effect of the molecular weight on the interfacial structures and blood compatibility. The amounts of intermediate water and fibrinogen adsorption were not affected by the molecular weight of PMEA...
January 23, 2019: Langmuir: the ACS Journal of Surfaces and Colloids
Hua Qiao, Bo Li, Huichao Zhang, Dan Liu, Haipeng Diao, Gongqin Sun, Jun Xie
Liposomes are a common delivery vehicle for drugs or biologicals, but some common surfactants used as liposome components may cause denaturation and malfunction of serum proteins and cell surface proteins. In this study, we examined the effects of liposome lipid didodecyldimethylammonium bromide (DDAB), nonionic polyoxyethylene sorbitan monooleate 80 (Tween 80), and the equimolar mixture on the properties of serum proteins. Bovine serum albumin was selected as the main model protein, and the effects of the DDAB, Tween 80, and a 1:1 mixture on its spectroscopic behavior were investigated...
October 2018: Journal of Photochemistry and Photobiology. B, Biology
M G Gorobets, L A Wasserman, A V Bychkova, M L Konstantinova, I G Plaschina, M A Rosenfeld
For the first time, with the aid of differential scanning calorimetry, the thermal denaturation of fibrinogen under induced oxidation was studied. All fibrinogen structural elements detected by DSC (D region, αC-domain, and E region) are subjected to oxidation. Structural changes in fibrinogen molecule were characterized by the denaturation temperature, denaturation enthalpy, and van't Hoff enthalpy.
May 2018: Doklady. Biochemistry and Biophysics
Lauren E-A Eyssen, Perina Vather, Laurelle Jackson, Phindile Ximba, Nicolas Biteau, Théo Baltz, Alain Boulangé, Philippe Büscher, Theresa H T Coetzer
African animal trypanosomosis (nagana) is caused by tsetse-transmitted protozoan parasites. Their cysteine proteases are potential chemotherapeutic and diagnostic targets. The N-glycosylated catalytic domain of Trypanosoma vivax cathepsin L-like cysteine protease, rTviCATLcat , was recombinantly expressed and purified from culture supernatants while native TviCATL was purified from T. vivax Y486 parasite lysates. Typical of Clan CA, family C1 proteases, TviCATL activity is sensitive to E-64 and cystatin and substrate specificity is defined by the S2 pocket...
July 2018: Molecular and Biochemical Parasitology
Nikolay A Barinov, Anna D Protopopova, Evgeniy V Dubrovin, Dmitry V Klinov
Fibrinogen denaturation is an important phenomenon in biology and medicine. It has been previously investigated with bulk methods and characterized by parameters, which refer to big protein ensembles. Here we provide a new insight into fibrinogen denaturation with a high-resolution single-molecule atomic force microscopy (AFM). The ultrastructure of individual fibrinogen molecules was studied after heating or extended contact with the highly oriented pyrolytic graphite surface (HOPG) modified with oligoglycine-hydrocarbon graphite modifier (GM)...
July 1, 2018: Colloids and Surfaces. B, Biointerfaces
Aijing Lu, Zhengzhong Wu, Xianglin Luo, Suming Li
Micelles of poly(ε-caprolactone)-b-poly(N,N-diethylaminoethyl methacrylate)/(N-(3-sulfopropyl-N-methacryloxyethy-N,N-diethylammonium betaine)) (PCL-PDEAPS) and poly(ε-caprolactone)-b-poly(ethylene glycol) (PCL-PEG) were prepared and characterized. The interactions of micelles with model proteins such as bovine serum albumin (BSA), lysozyme (Ly), fibrinogen (Fg) and plasma were studied from adsorption quantity, micelle size, polydispersity index (PDI) and zeta-potential measurements. The adsorption quantity of negatively charged proteins on PCL-PDEAPS micelles containing zwitterionic sulfobetaine is larger than on non-ionic PEG-PCL micelles...
July 1, 2018: Colloids and Surfaces. B, Biointerfaces
Yongping Deng, Xiaolan Liu, Priti Katrolia, Narasimha Kumar Kopparapu, Xiqun Zheng
In this study, we have isolated and characterized a fibrinolytic enzyme from the GRAS (Generally Recognized as Safe) fungus, Neurospora sitophila. The enzyme was purified by fractional ammonium sulfate precipitation, hydrophobic interaction, ion exchange and gel filtration chromatography to 45.2 fold with a specific activity of 415.6U/mg protein. The native molecular mass of the enzyme was 49kDa, while the denatured molecular mass was 30kDa and 17.5kDa, indicating that the enzyme was a hetero-dimer. It was optimally active at 50°C and pH 7...
April 1, 2018: International Journal of Biological Macromolecules
Valeria Genoud, Paula G Quintana, Silvana Gionco, Alicia Baldessari, Irene Quintana
Homocysteine and its cyclic ester homocysteine thiolactone (HTL) have been involved in the detrimental consequences associated to hyperhomocysteinemia, an independent risk factor for vascular diseases. HTL reacts with protein lysine residues in a process named N-homocysteinylation. The aim of our study was to evaluate the in vitro effects of HTL on the fibrinogen through electrophoretic methods. Fibrinogen was incubated with HTL at different molar ratios and structural changes of the protein were assessed by polyacrylamide gel electrophoresis (PAGE), capillary zone electrophoresis (CZE) and capillary isoelectric focusing (CIEF)...
January 2018: Journal of Thrombosis and Thrombolysis
Kazuki Fukushima, Yuto Inoue, Yuta Haga, Takayuki Ota, Kota Honda, Chikako Sato, Masaru Tanaka
We developed a biodegradable polycarbonate that demonstrates antithrombogenicity and vascular cell adhesion via organocatalytic ring-opening polymerization of a trimethylene carbonate (TMC) analogue bearing a methoxy group. The monoether-tagged polycarbonate demonstrates a platelet adhesion property that is 93 and 89% lower than those of poly(ethylene terephthalate) and polyTMC, respectively. In contrast, vascular cell adhesion properties of the polycarbonate are comparable to those controls, indicating a potential for selective cell adhesion properties...
November 13, 2017: Biomacromolecules
S Sanooja Pinki, Ganesh Mohan, Aboobacker Mohamed Rafi, Susheela Jacob Innah, Tom Thomas
BACKGROUND: Fresh frozen plasma (FFP) should be thawed before transfusing to the patient. Prolonged or uncontrolled thawing can denature plasma proteins. The potential risk of contamination by wet thawing had always been a point of concern. AIMS: Here, we compared and evaluated the effect of thawing on clotting factor activities by two different methods (wet and dry) and other factors such as risk of bacterial contamination, throughput, turnaround time, and efficacy of thawing...
July 2017: Asian Journal of Transfusion Science
Ying Yang, Xiangyang Li, Hua Qiu, Ping Li, Pengkai Qi, Manfred F Maitz, Tianxue You, Ru Shen, Zhilu Yang, Wenjie Tian, Nan Huang
Sustained and controllable release characteristics are pivotal factors for novel drug delivery technologies. TiO2 nanotube arrays prepared by self-ordering electrochemical anodization are attractive for the development of biomedical devices for local drug delivery applications. In this work, several layers of polydopamine (PDA) were deposited to functionalize TiO2 nanotube arrays. The anticoagulant drug bivalirudin (BVLD) was used as a model drug. PDA extended the release period of BVLD and maintained a sustained release kinetic...
March 7, 2018: ACS Applied Materials & Interfaces
Karin Fromell, Yi Yang, Kristina Nilsson Ekdahl, Bo Nilsson, Mattias Berglin, Hans Elwing
In previous investigations, the authors have examined the adsorption of albumin, immunoglobulin, and fibrinogen to a series of acrylate polymers with different backbone and side-group flexibility. The authors showed that protein adsorption to acrylates with high flexibility, such as poly(lauryl methacrylate) (PLMA), tends to preserve native conformation. In the present study, the authors have continued this work by examining the conformational changes that occur during the binding of complement factor 3 (C3) and coagulation factor XII (FXII)...
June 21, 2017: Biointerphases
Kazuhiro Sato, Shingo Kobayashi, Asuka Sekishita, Miyuki Wakui, Masaru Tanaka
A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple two-step reaction, and then the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the nonthrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and enzyme-linked immunosorbent assay...
May 8, 2017: Biomacromolecules
Claudia Bonechi, Stefania Lamponi, Alessandro Donati, Gabriella Tamasi, Marco Consumi, Gemma Leone, Claudio Rossi, Agnese Magnani
The effect of resveratrol (RSV) in inhibiting platelet adhesion and aggregation, as well as fibrinogen (FBG) conformational changes promoted by epinephrine (EP), were studied, by using complementary experimental techniques. NMR and IR spectroscopies were used to investigate possible protective effects by RSV towards FBG, in presence of EP. The protective effect of RSV towards FBG was highlighted by spin nuclear relaxation experiments that were interpreted for determining the thermodynamic equilibrium constants of FBG-EP interaction, and by infrared measurements, that showed EP-induced conformational changes of FBG...
March 2017: Biophysical Chemistry
Dawn Coverley, Gillian Higgins, Daniel West, Oliver T Jackson, Adam Dowle, Aidan Haslam, Eve Ainscough, Rebecca Chalkley, John White
OBJECTIVES: Non-invasive tests for early detection of lung cancer are an important unmet clinical need. CIZ1b plasma biomarker can discriminate stage 1 lung cancer from within high-risk groups with clinically useful accuracy, with ROC AUCs in excess of 0.9 for two independent retrospective cohorts, and could therefore meet this need. Our aim was to characterise the native state of the biomarker and develop a quantitative immunoassay. DESIGN AND METHODS: Selective denaturation, preparative electrophoresis and mass spectrometry of human plasma were used to characterise the biomarker and interaction partners...
April 2017: Clinical Biochemistry
Piotr Kuta, Barbara Hauck-Dlimi, Julian Strobel, Robert Zimmermann, Reinhold Eckstein
BACKGROUND: Uncontrolled hemorrhage in polytrauma patients usually results in rapid need of blood products. Despite the shorter thawing times of microwave devices for heating fresh frozen plasma (FFP), their use has remained controversial, and just a few laboratory analyses have been published on this topic. The aim of this study was to analyse the quality of clotting factors immediately after thawing FFP with a microwave device and after 48-hour post thaw storage at 4 degrees C. METHODS: 24 FFP units of all four ABO blood groups (six of each blood group) were thawed with a Transfusio-therm 2000 and later stored at 4 degrees C for 48 hours...
2016: Clinical Laboratory
Nikolay A Barinov, Valery V Prokhorov, Evgeniy V Dubrovin, Dmitry V Klinov
Different graphitic materials are either already used or believed to be advantageous in biomedical and biotechnological applications, e.g., as biomaterials or substrates for sensors. Most of these applications or associated important issues, such as biocompatibility, address the problem of adsorption of protein molecules and, in particular the conformational state of the adsorbed protein molecule on graphite. High-resolution AFM demonstrates highly oriented pyrolytic graphite (HOPG) induced denaturation of four proteins of blood plasma, such as ferritin, fibrinogen, human serum albumin (HSA) and immunoglobulin G (IgG), at a single molecule level...
October 1, 2016: Colloids and Surfaces. B, Biointerfaces
Rami N Foster, Elisa T Harrison, David G Castner
The adsorption of single-component bovine serum albumin (BSA), bovine fibrinogen (Fgn), and bovine immunoglobulin G (IgG) films as well as multicomponent bovine plasma films onto bare and sodium styrenesulfonate (NaSS)-grafted gold substrates was characterized. The adsorption isotherms, measured via X-ray photoelectron spectroscopy, showed that at low solution concentrations all three single-component proteins adsorb with higher affinity onto gold surfaces compared to NaSS surfaces. However, at higher concentrations, NaSS surfaces adsorb the same or more total protein than gold surfaces...
April 5, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Stephan Köhler, Friederike Schmid, Giovanni Settanni
Fibrinogen, a blood glycoprotein of vertebrates, plays an essential role in blood clotting by polymerizing into fibrin when activated. Upon adsorption on material surfaces, it also contributes to determine their biocompatibility and has been implicated in the onset of thrombosis and inflammation at medical implants. Here we present the first fully atomistic simulations of the initial stages of the adsorption process of fibrinogen on mica and graphite surfaces. The simulations reveal a weak adsorption on mica that allows frequent desorption and reorientation events...
December 8, 2015: Langmuir: the ACS Journal of Surfaces and Colloids
Maria Scuotto, Elisa Rivieccio, Alessia Varone, Daniela Corda, Mariarosaria Bucci, Valentina Vellecco, Giuseppe Cirino, Antonella Virgilio, Veronica Esposito, Aldo Galeone, Nicola Borbone, Michela Varra, Luciano Mayol
Many antiproliferative G-quadruplexes (G4s) arise from the folding of GT-rich strands. Among these, the Thrombin Binding Aptamer (TBA), as a rare example, adopts a monomolecular well-defined G4 structure. Nevertheless, the potential anticancer properties of TBA are severely hampered by its anticoagulant action and, consequently, no related studies have appeared so far in the literature. We wish to report here that suitable chemical modifications in the TBA sequence can preserve its antiproliferative over anticoagulant activity...
September 18, 2015: Nucleic Acids Research
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