Yanmei Zhang, Qifan Hu, Yuquan Pei, Hao Luo, Zixuan Wang, Xinxin Xu, Qing Zhang, Jianli Dai, Qianqian Wang, Zilian Fan, Yongcong Fang, Min Ye, Binhan Li, Mailin Chen, Qi Xue, Qingfeng Zheng, Shulin Zhang, Miao Huang, Ting Zhang, Jin Gu, Zhuo Xiong
Cancer models play critical roles in basic cancer research and precision medicine. However, current in vitro cancer models are limited by their inability to mimic the three-dimensional architecture and heterogeneous tumor microenvironments (TME) of in vivo tumors. Here, we develop an innovative patient-specific lung cancer assembloid (LCA) model by using droplet microfluidic technology based on a microinjection strategy. This method enables precise manipulation of clinical microsamples and rapid generation of LCAs with good intra-batch consistency in size and cell composition by evenly encapsulating patient tumor-derived TME cells and lung cancer organoids inside microgels...
April 20, 2024: Nature Communications