Andrew D Berti, Erin Theisen, John-Demian Sauer, Poochit Nonejuie, Joshua Olson, Joseph Pogliano, George Sakoulas, Victor Nizet, Richard A Proctor, Warren E Rose
The activity of daptomycin (DAP) against methicillin-resistant Staphylococcus aureus (MRSA) is enhanced in the presence of β-lactam antibiotics. This effect is more pronounced with β-lactam antibiotics that exhibit avid binding to penicillin binding protein 1 (PBP1). Here, we present evidence that PBP1 has a significant role in responding to DAP-induced stress on the cell. Expression of the pbpA transcript, encoding PBP1, was specifically induced by DAP exposure whereas expression of pbpB, pbpC, and pbpD, encoding PBP2, PBP3, and PBP4, respectively, remained unchanged...
January 2016: Antimicrobial Agents and Chemotherapy