keyword
https://read.qxmd.com/read/34978891/is-the-success-of-cefazolin-plus-ertapenem-in-methicillin-susceptible-staphylococcus-aureus-bacteremia-based-on-release-of-interleukin-1-beta
#21
JOURNAL ARTICLE
Dan Smelter, Mary Hayney, George Sakoulas, Warren Rose
Cefazolin and ertapenem have been shown to be an effective salvage regimen for refractory methicillin-susceptible Staphylococcus aureus bacteremia. Our findings suggest cefazolin plus ertapenem in vitro stimulates interleukin-1β release from peripheral blood monocytes both with and without S. aureus presence. This IL-1β augmentation was primarily driven by ertapenem. These findings support further exploration of cefazolin plus ertapenem in MSSA bacteremia and may partially explain its marked potency in vivo despite modest synergy in vitro ...
February 15, 2022: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/34803896/acute-psychosis-due-to-anti-n-methyl-d-aspartate-receptor-encephalitis-following-covid-19-vaccination-a-case-report
#22
Patrick Flannery, Ingrid Yang, Madjid Keyvani, George Sakoulas
Anti-N-methyl D-aspartate (NMDA) receptor (anti-NMDAR) encephalitis has been reported after SARS-CoV-2 infection, but not after SARS-CoV-2 vaccination. We report the first known case of anti-NMDAR encephalitis after SARS-CoV-2 immunization in a young female presenting with acute psychosis, highlighting a rare potential immunological complication of vaccination against SARS-CoV-2 that is currently being distributed worldwide. The patient presented initially with anxiety and hypochondriacal delusions which progressed to psychosis and catatonia but returned to baseline with aggressive immunomodulatory therapy consisting of intravenous immunoglobulin, high-dose glucocorticoids, and rituximab...
2021: Frontiers in Neurology
https://read.qxmd.com/read/34743174/universal-activity-based-labeling-method-for-ammonia-and-alkane-oxidizing-bacteria
#23
JOURNAL ARTICLE
Dimitra Sakoula, Garrett J Smith, Jeroen Frank, Rob J Mesman, Linnea F M Kop, Pieter Blom, Mike S M Jetten, Maartje A H J van Kessel, Sebastian Lücker
The advance of metagenomics in combination with intricate cultivation approaches has facilitated the discovery of novel ammonia-, methane-, and other short-chain alkane-oxidizing microorganisms, indicating that our understanding of the microbial biodiversity within the biogeochemical nitrogen and carbon cycles still is incomplete. The in situ detection and phylogenetic identification of novel ammonia- and alkane-oxidizing bacteria remain challenging due to their naturally low abundances and difficulties in obtaining new isolates from complex samples...
April 2022: ISME Journal
https://read.qxmd.com/read/34683376/bacterial-membrane-derived-vesicles-attenuate-vancomycin-activity-against-methicillin-resistant-staphylococcus-aureus
#24
JOURNAL ARTICLE
Monika Kumaraswamy, Kamilla Wiull, Bishnu Joshi, George Sakoulas, Armin Kousha, Gustav Vaaje-Kolstad, Mona Johannessen, Kristin Hegstad, Victor Nizet, Fatemeh Askarian
Methicillin-resistant Staphylococcus aureus (MRSA) has evolved numerous antimicrobial resistance mechanisms and is identified as a serious public health threat by the World Health Organization and U.S. Centers for Disease Control and Prevention. The glycopeptide vancomycin (VAN) remains a cornerstone of therapy for severe MRSA infections despite increasing reports of therapeutic failure in hospitalized patients with bacteremia or pneumonia. Recently, the role of released bacterial-derived membrane vesicles (MVs) in antibiotic resistance has garnered attention...
September 29, 2021: Microorganisms
https://read.qxmd.com/read/34476284/vancomycin-or-daptomycin-for-outpatient-parenteral-antibiotic-therapy-does-it-make-a-difference-in-patient-satisfaction
#25
JOURNAL ARTICLE
Katherine H Wu, George Sakoulas, Matthew Geriak
A 5-question telephone survey was administered to compare satisfaction between patients receiving vancomycin vs daptomycin outpatient parenteral antimicrobial therapy (OPAT). Twenty-seven patients completed the survey (40%). Vancomycin had higher daily interference score than daptomycin ( P = .03). All patients receiving daptomycin reported a satisfaction score ≥8/10, as compared to 67% of patients who received vancomycin ( P < .03). OPAT antibiotics with less cumbersome administration regimens may translate into higher patient satisfaction and quicker return to life normalcy...
August 2021: Open Forum Infectious Diseases
https://read.qxmd.com/read/34172889/environmental-conditions-dictate-differential-evolution-of-vancomycin-resistance-in-staphylococcus-aureus
#26
JOURNAL ARTICLE
Henrique Machado, Yara Seif, George Sakoulas, Connor A Olson, Ying Hefner, Amitesh Anand, Ying Z Jones, Richard Szubin, Bernhard O Palsson, Victor Nizet, Adam M Feist
While microbiological resistance to vancomycin in Staphylococcus aureus is rare, clinical vancomycin treatment failures are common, and methicillin-resistant S. aureus (MRSA) strains isolated from patients after prolonged vancomycin treatment failure remain susceptible. Adaptive laboratory evolution was utilized to uncover mutational mechanisms associated with MRSA vancomycin resistance in a physiological medium as well as a bacteriological medium used in clinical susceptibility testing. Sequencing of resistant clones revealed shared and media-specific mutational outcomes, with an overlap in cell wall regulons (walKRyycHI, vraSRT)...
June 25, 2021: Communications Biology
https://read.qxmd.com/read/34160473/clinical-efficacy-of-patients-with-secondary-bacteremia-treated-with-omadacycline-results-from-phase-3-acute-bacterial-skin-and-skin-structure-infections-and-community-acquired-bacterial-pneumonia-studies
#27
JOURNAL ARTICLE
George Sakoulas, Paul B Eckburg, Maria Amodio-Groton, Amy Manley, Evan Tzanis, Anita F Das, Robert Noble, Paul C McGovern
In this post hoc analysis of the 63 patients with secondary bacteremia enrolled in the 3 omadacycline phase 3 studies of acute bacterial skin/skin structure infections (ABSSSI) and community-acquired bacterial pneumonia (CABP), we determined that omadacycline is a viable therapeutic option for appropriate patients with secondary bacteremia.
June 2021: Open Forum Infectious Diseases
https://read.qxmd.com/read/33993226/current-paradigms-of-combination-therapy-in-methicillin-resistant-staphylococcus-aureus-mrsa-bacteremia-does-it-work-which-combination-and-for-which-patients
#28
REVIEW
Warren Rose, Michael Fantl, Matthew Geriak, Victor Nizet, George Sakoulas
The last several years have seen an emergence of literature documenting the utility of combination antimicrobial therapy, particularly in the salvage of refractory methicillin-resistant Staphylococcus aureus (MRSA) bacteremia. Recent clinical data are shaping conundrums of which regimens may be more beneficial, which can be potentially harmful, and which subset of patients stand to benefit from more aggressive treatment regimens than called for by current standards. In addition, the incorporation of combination therapy for MRSA bacteremia should be accompanied by the reminder that antimicrobial therapy does not need to be uniform for the entire duration, with an early intensive phase in high inoculum infections (eg, with combination therapy), followed by a consolidation phase (ie, monotherapy)...
December 16, 2021: Clinical Infectious Diseases
https://read.qxmd.com/read/33977506/randomized-prospective-open-label-study-shows-no-impact-on-clinical-outcome-of-adding-losartan-to-hospitalized-covid-19-patients-with-mild-hypoxemia
#29
JOURNAL ARTICLE
Matthew Geriak, Fadi Haddad, Ravina Kullar, Kristina L Greenwood, MacKenzie Habib, Cole Habib, David Willms, George Sakoulas
INTRODUCTION: Despite considerable scientific debate, there have been no prospective clinical studies on the effects of angiotensin II receptor blockers (ARBs) on the course of COVID-19 infection. Losartan is the ARB that was chosen to be tested in this study. METHODS: Patients with COVID-19 and mild hypoxia (receipt of ≤ 3 L/min O2 by nasal cannula) admitted to three hospitals were randomized in a 1:1 ratio within 72 h of SARS-CoV-2 nucleic acid testing confirmation to prospectively receive standard of care (SOC) alone or SOC plus losartan 12...
May 11, 2021: Infectious Diseases and Therapy
https://read.qxmd.com/read/33966075/ticagrelor-increases-platelet-mediated-staphylococcus-aureus-killing-resulting-in-clearance-of-bacteremia
#30
JOURNAL ARTICLE
Erlinda R Ulloa, Satoshi Uchiyama, Robert Gillespie, Victor Nizet, George Sakoulas
Platelets are a critical immune defense against Staphylococcus aureus bloodstream infections. Staphylococcus aureus α-toxin is a virulence factor that decreases platelet viability and accelerates platelet clearance. It has been shown that ticagrelor blocks α-toxin-mediated platelet injury and resulting thrombocytopenia, protecting mice in a lethal S. aureus sepsis model. We now present the use of ticagrelor as adjunctive therapy in a patient with a S. aureus endovascular infection and thrombocytopenia, associated with restoration of platelet count and bacteremia clearance...
November 16, 2021: Journal of Infectious Diseases
https://read.qxmd.com/read/33762439/repurposed-drugs-block-toxin-driven-platelet-clearance-by-the-hepatic-ashwell-morell-receptor-to-clear-staphylococcus-aureus-bacteremia
#31
JOURNAL ARTICLE
Josh Sun, Satoshi Uchiyama, Joshua Olson, Yosuke Morodomi, Ingrid Cornax, Nao Ando, Yohei Kohno, May M T Kyaw, Bernice Aguilar, Nina M Haste, Sachiko Kanaji, Taisuke Kanaji, Warren E Rose, George Sakoulas, Jamey D Marth, Victor Nizet
Staphylococcus aureus (SA) bloodstream infections cause high morbidity and mortality (20 to 30%) despite modern supportive care. In a human bacteremia cohort, we found that development of thrombocytopenia was correlated to increased mortality and increased α-toxin expression by the pathogen. Platelet-derived antibacterial peptides are important in bloodstream defense against SA, but α-toxin decreased platelet viability, induced platelet sialidase to cause desialylation of platelet glycoproteins, and accelerated platelet clearance by the hepatic Ashwell-Morell receptor (AMR)...
March 24, 2021: Science Translational Medicine
https://read.qxmd.com/read/33316806/use-of-intravenous-immunoglobulin-prevagen-or-octagam-for-the-treatment-of-covid-19-retrospective-case-series
#32
JOURNAL ARTICLE
Felix J F Herth, George Sakoulas, Fadi Haddad
Treatment with immunomodulators, such as intravenous immunoglobulin (IVIG), may attenuate inflammatory responses observed in the severe stages of acute respiratory distress syndrome (ARDS) caused by coronavirus disease 19 (COVID-19). We retrospectively evaluated the clinical courses of 12 COVID-19 patients who received IVIG at various stages of their illness, including within the first 72 h of clinical presentation, after initiation of mechanical ventilation, and after prolonged ventilation and ICU stay. The patients included 9 men and 3 women with a median age of 50 years (range 23-74), median Charlson Comorbidity Score of 2 (range 0-7), and median Acute Physiology and Chronic Health Evaluation Score of 13 (range 5-33) at the time of IVIG...
2020: Respiration; International Review of Thoracic Diseases
https://read.qxmd.com/read/33225306/intravenous-immunoglobulin-plus-methylprednisolone-mitigate-respiratory-morbidity-in-coronavirus-disease-2019
#33
JOURNAL ARTICLE
George Sakoulas, Matthew Geriak, Ravina Kullar, Kristina L Greenwood, MacKenzie Habib, Anuja Vyas, Mitra Ghafourian, Venkata Naga Kiran Dintyala, Fadi Haddad
Dysregulated neutrophil and platelet interactions mediate immunothrombosis and cause lung injury in coronavirus disease 2019. IV immunoglobulin modulates neutrophil activation through FcγRIII binding. We hypothesized that early therapy with IV immunoglobulin would abrogate immunothrombosis and improve oxygenation and reduce progression to mechanical ventilation in coronavirus disease 2019 pneumonia. Design: Prospective randomized open label. Setting: Inpatient hospital...
November 2020: Critical care explorations
https://read.qxmd.com/read/33188298/enrichment-and-physiological-characterization-of-a-novel-comammox-nitrospira-indicates-ammonium-inhibition-of-complete-nitrification
#34
JOURNAL ARTICLE
Dimitra Sakoula, Hanna Koch, Jeroen Frank, Mike S M Jetten, Maartje A H J van Kessel, Sebastian Lücker
The recent discovery of bacteria within the genus Nitrospira capable of complete ammonia oxidation (comammox) demonstrated that the sequential oxidation of ammonia to nitrate via nitrite can also be performed within a single bacterial cell. Although comammox Nitrospira exhibit a wide distribution in natural and engineered ecosystems, information on their physiological properties is scarce due to the limited number of cultured representatives. Additionally, most available genomic information is derived from metagenomic sequencing and high-quality genomes of Nitrospira in general are limited...
November 13, 2020: ISME Journal
https://read.qxmd.com/read/33020155/imipenem-cilistatin-and-fosfomycin-for-refractory-methicillin-resistant-staphylococcus-aureus-infection-a-novel-combination-therapy
#35
JOURNAL ARTICLE
George Sakoulas
Given that it unlikely that randomized clinical trials will yield the answers in treating the most challenging bacteremic infections caused by methicillin-resistant Staphylococcus aureus , clinicians, microbiologists, and pharmacists will have to cooperate to discover novel ways in selecting successful individualized antimicrobial therapy for these patients. An example of such a strategy was demonstrated in the identification and utilization of imipenem/cilistatin plus fosfomycin to treat a particularly recalcitrant MRSA bacteremia and spinal abscess...
October 5, 2020: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/32888495/mortality-risk-profiling-of-staphylococcus-aureus-bacteremia-by-multi-omic-serum-analysis-reveals-early-predictive-and-pathogenic-signatures
#36
JOURNAL ARTICLE
Jacob M Wozniak, Robert H Mills, Joshua Olson, J R Caldera, Gregory D Sepich-Poore, Marvic Carrillo-Terrazas, Chih-Ming Tsai, Fernando Vargas, Rob Knight, Pieter C Dorrestein, George Y Liu, Victor Nizet, George Sakoulas, Warren Rose, David J Gonzalez
Staphylococcus aureus bacteremia (SaB) causes significant disease in humans, carrying mortality rates of ∼25%. The ability to rapidly predict SaB patient responses and guide personalized treatment regimens could reduce mortality. Here, we present a resource of SaB prognostic biomarkers. Integrating proteomic and metabolomic techniques enabled the identification of >10,000 features from >200 serum samples collected upon clinical presentation. We interrogated the complexity of serum using multiple computational strategies, which provided a comprehensive view of the early host response to infection...
September 3, 2020: Cell
https://read.qxmd.com/read/32818143/antibiotics-and-innate-immunity-a-cooperative-effort-toward-the-successful-treatment-of-infections
#37
JOURNAL ARTICLE
Andrew Berti, Warren Rose, Victor Nizet, George Sakoulas
Despite the common ancestry of antimicrobial and immunological science, a divergence driven by artificially construed paradigms in microbiology has placed limits on how we understand the mechanisms of antibiotics in vivo. We summarize recent updates on data that shed light on how antibiotics interact with components of innate immunity.
August 2020: Open Forum Infectious Diseases
https://read.qxmd.com/read/32354985/genome-sequence-comparison-of-staphylococcus-aureus-tx0117-and-a-beta-lactamase-cured-derivative-shows-increased-cationic-peptide-resistance-accompanying-mutations-in-rela-and-mnaa
#38
JOURNAL ARTICLE
Mia Jade Sales, George Sakoulas, Richard Szubin, Bernhard Palsson, Cesar Arias, Kavindra V Singh, Barbara E Murray, Jonathan M Monk
Staphylococcus aureus strain TX0117 is a methicillin-susceptible bacterium with type A beta-lactamase exhibiting a high cefazolin inoculum effect. TX0117 was cured of blaZ , yielding TX0117c with increased antimicrobial peptide resistance. The sequencing and genome assembly of TX0117 elucidate six mutations between TX0117 and TX0117c, including relA truncation and mnA_1 substitution.
April 30, 2020: Microbiology Resource Announcements
https://read.qxmd.com/read/32234776/genetic-determinants-enabling-medium-dependent-adaptation-to-nafcillin-in-methicillin-resistant-staphylococcus-aureus
#39
JOURNAL ARTICLE
Michael J Salazar, Henrique Machado, Nicholas A Dillon, Hannah Tsunemoto, Richard Szubin, Samira Dahesh, Joseph Pogliano, George Sakoulas, Bernhard O Palsson, Victor Nizet, Adam M Feist
Antimicrobial susceptibility testing standards driving clinical decision-making have centered around the use of cation-adjusted Mueller-Hinton broth (CA-MHB) as the medium with the notion of supporting bacterial growth, without consideration of recapitulating the in vivo environment. However, it is increasingly recognized that various medium conditions have tremendous influence on antimicrobial activity, which in turn may have major implications on the ability of in vitro susceptibility assays to predict antibiotic activity in vivo...
March 31, 2020: MSystems
https://read.qxmd.com/read/31938716/multicenter-cohort-of-patients-with-methicillin-resistant-staphylococcus-aureus-bacteremia-receiving-daptomycin-plus-ceftaroline-compared-with-other-mrsa-treatments
#40
JOURNAL ARTICLE
Erin K McCreary, Ravina Kullar, Matthew Geriak, Evan J Zasowski, Khulood Rizvi, Lucas T Schulz, Krista Ouellette, Logan Vasina, Fadi Haddad, Michael J Rybak, Marcus J Zervos, George Sakoulas, Warren E Rose
Background: Daptomycin and ceftaroline (DAP-CPT) have been used for persistent methicillin-resistant Staphylococcus aureus bacteremia (MRSAB), but have rarely been compared with other therapies. This study provides an exploratory analysis of patients placed on DAP-CPT vs standard of care (SOC) for MRSAB. Methods: This is a retrospective, matched cohort study MRSAB patients at 4 hospitals in the United States. Patients receiving DAP-CPT for ≥72 hours at any point in therapy were matched 2:1 when possible, 1:1 otherwise, to SOC, first by infection source, then age and renal function...
January 2020: Open Forum Infectious Diseases
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