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Paper Based Analytical Device

Rita Soares, Verónica C Martins, Rita Macedo, Filipe A Cardoso, Sofia A M Martins, Diogo M Caetano, Pedro H Fonseca, Vânia Silvério, Susana Cardoso, Paulo P Freitas
The growing need for biological information at the single cell level has driven the development of improved cytometry technologies. Flow cytometry is a particularly powerful method that has evolved over the past few decades. Flow cytometers have become essential instruments in biomedical research and routine clinical tests for disease diagnosis, prognosis, and treatment monitoring. However, the increasing number of cellular parameters unveiled by genomic, proteomic, and metabolomic data platforms demands an augmented multiplexability...
February 19, 2019: Analytical and Bioanalytical Chemistry
Grenalynn C Ilacas, Alexis Basa, Katherine J Nelms, Joshua D Sosa, Yangyang Liu, Frank A Gomez
This paper describes the development of two microfluidic paper-based analytical devices (μPADs), one well-based and the other based on a lateral flow assay (LFA) configuration, to detect glucose via a colorimetric assay using the solid metal-organic framework (MOF) Zr-PCN-222(Fe), to encapsulate glucose oxidase (GOx). The well-based platform consisted of laminate sheets and multiple layers of wax-printed chromatography paper. Solutions of KI and glucose placed into the well flowed through the device and reacted with the GOx@MOF species sandwiched between the paper layers realizing a yellow-brown color...
May 9, 2019: Analytica Chimica Acta
Gerald Theillet, Gilda Grard, Mathilde Galla, Carine Maisse, Margot Enguehard, Marie Cresson, Pascal Dalbon, Isabelle Leparc Leparc-Goffart, Frederic Bedin
The incidence of arbovirus infections has increased dramatically in recent decades, affecting hundreds of millions of people each year. The Togaviridae family includes the chikungunya virus (CHIKV), which is typically transmitted by Aedes mosquitoes and causes a wide range of symptoms from flu-like fever to severe arthralgia. Although conventional diagnostic tests can provide early diagnosis of CHIKV infections, access to these tests is often limited in developing countries. Consequently, there is an urgent need to develop efficient, affordable, simple, rapid, and robust diagnostic tools that can be used in point-of-care settings...
February 8, 2019: Journal of Medical Virology
Julaluk Noiphung, Wanida Laiwattanapaisal
Point-of-care testing (POCT) for uropathogen detection and chemical screening has great benefits for the diagnosis of urinary tract infections (UTIs). The goal of this study was to develop a portable and inexpensive paper-based analytical device (PAD) for cultivating bacteria in situ and rapidly testing for nitrite on the same device. The PAD was fabricated using a wax printing technique to create a pattern on Whatman No. 1 filter paper, which was then combined with a cotton sheet to support bacterial growth...
February 7, 2019: Scientific Reports
Yasir Mehmood, Lei Zhang, Anna Förster
Machine-type communication (MTC) is an emerging communication trend where intelligent machines are capable of communicating with each other without human intervention. Mobile cellular networks, with their wide range, high data rates, and continuously decreasing costs, offer a good infrastructure for implementing them. However, power consumption is a great issue, which has recently been addressed by 3GPP (3rd Generation Partnership Project) by defining power-saving mechanisms. In this paper, we address the problem of modeling these power-saving mechanisms...
February 1, 2019: Sensors
Hongliang Lu, Bin Lu, Yuming Zhang, Yimen Zhang, Zhijun Lv
The practical use of tunnel field-effect transistors is retarded by the low on-state current. In this paper, the energy-band engineering of InAs/Si heterojunction and novel device structure of source-pocket concept are combined in a single tunnel field-effect transistor to extensively boost the device performance. The proposed device shows improved tunnel on-state current and subthreshold swing. In addition, analytical potential model for the proposed device is developed and tunneling current is also calculated...
February 1, 2019: Nanomaterials
Liping Xie, Xingyu Zi, Hedele Zeng, Jianjun Sun, Lisheng Xu, Shuo Chen
Despite that microfluidic paper-based analytical devices (μPADs) provide effective analytical platforms for point-of-care diagnosis in resource-limited areas, it remains challenging to achieve simple and low-cost fabrication of μPADs. A novel method for fabrication of μPADs is developed in this study using a folded polydimethylsiloxane (PDMS)-coated paper mask with a specific pattern to form a sandwich structure with inserted chromatographic paper. PDMS penetrates the target paper from the front and the back sides, and then is cured in the target paper to form legible channels...
April 11, 2019: Analytica Chimica Acta
Suchanat Boonkaew, Sudkate Chaiyo, Sakda Jampasa, Sirirat Rengpipat, Weena Siangproh, Orawon Chailapakul
An origami paper-based electrochemical immunoassay for C-reactive protein (CRP) detection is described. The assay integrates multiple steps of electrode modification into a single device. A graphene-modified screen-printed carbon electrode (G/SPCE) was employed to enhance sensitivity. Gold nanoparticles were first electrodeposited onto the G/SPCE, followed by a self-assembled monolayer of L-cysteine. The capture anti-CRP was then covalently immobilized on the modified electrode. CRP was quantified by measuring the changes in the charge-transfer resistance of the electrode by using hexacyanoferrate as the redox probe...
February 2, 2019: Mikrochimica Acta
Tianyu Dong, Guan A Wang, Feng Li
Rapid, low-cost, and sensitive nucleic acid detection and quantification assays enabled by microfluidic paper-based analytical devices (μPADs) hold great promise for point-of-care disease diagnostics and field-based molecular tests. Through the capillary action in μPAD, flexible manipulation of nucleic acid samples can be achieved without the need for external pumps or power supplies, making it possible to fabricate highly integrated sample-to-answer devices that streamline the nucleic acid extraction, separation, concentration, amplification, and detection...
February 1, 2019: Analytical and Bioanalytical Chemistry
Lei Liu, Mao-Rong Xie, Yu-Zhu Chen, Zhi-Yong Wu
On-line enrichment is effective for improving the sensitivity of paper-based analytical devices (PADs). Electrokinetic stacking of ionic species - anionic or cationic species, respectively, on a paper-based fluidic channel has been well demonstrated in the literature. In this work, we further demonstrated that both anionic and cationic species can be electrokinetically stacked and separated simultaneously on the same paper fluidic channel. The feasibility of the proposed method was visually demonstrated by using a colored cationic probe of Rhodamine 6G and an anionic probe of Brilliant Blue...
February 1, 2019: Lab on a Chip
Yoshiki Soda, Daniel Citterio, Eric Bakker
A distance-based analysis of potassium ion (K+) is introduced that is performed on a microfluidic paper-based analyti-cal device (µPAD) coupled to an ion-selective capillary sensor. The concept is based on two sequential steps, the selec-tive replacement of analyte ion with an ionic dye, and the detection of this dye in a distance-based readout on paper. To achieve the first step, the capillary sensor holds a poly (vinyl chloride) (PVC) membrane film layer plasticized by dioctyl sebacate (DOS) that contains the potassium ionophore valinomycin, a lipophilic cation-exchanger and the ion-ic indicator Thioflavin T (ThT) on its inner wall...
January 31, 2019: ACS Sensors
Annachiara D'Urso, Giuliana Cangemi, Sebastiano Barco, Pasquale Striano, Antonio DʼAvolio, Ugo de Grazia
BACKGROUND: Therapeutic drug monitoring (TDM) of antiepileptic drugs (AEDs) is commonly performed on plasma or serum. The use of Dried Plasma Spots (DPS) could represent a useful tool to facilitate sample shipment to reference laboratories. In this paper, the authors describe the application of a commercially available UHPLC-MS/MS method for the determination of nine commonly prescribed AEDs (levetiracetam, lacosamide, topiramate, ethosuximide, lamotrigine, rufinamide, zonisamide, primidone, oxcarbazepine and its active metabolite 10-OH-monohydroxycarbazepine) to DPS collected on Dried Sample Spot Devices (DSSD)...
January 25, 2019: Therapeutic Drug Monitoring
Qinlei Liu, Yao Lin, Jing Xiong, Li Wu, Xiandeng Hou, Kailai Xu, Chengbin Zheng
A disposable, instrument-free, height readout paper-based analytical device (HR-PAD) based on a paper strip inkjet-printed with CdTe QDs was developed for the sensitive speciation analysis of Ag+ and AgNPs in commercial products. When the paper strip is immersed into a sample solution, capillary action draws it over the surface and any Ag+ in the solution quenches the fluorescence of CdTe QDs via a cation exchange reaction between Ag+ and the CdTe QDs, with the height of the quenched band being proportional to the concentration of Ag+...
January 28, 2019: Analytical Chemistry
Tiago Augusto Catelani, Dayana Borges Bittar, Leonardo Pezza, Helena Redigolo Pezza
New and reliable methodologies are described for the determination of amino acids in gym supplements, offering rapid and clean analysis, with low generation of waste. The proposed methods are based on the reaction between ninhydrin and amino acids in acetate buffer medium (pH 4.75). Experimental design tools were used to optimize the analytical conditions. The linear range obtained for both methodologies was 20.0-350.0 mg L-1 . The detection (LOD) and quantification (LOQ) limits were 6.2 and 21.0 mg L-1 , respectively, for diffuse reflectance using a paper filter with hydrophobic barriers to increase the sensitivity and homogeneity of the colored product, and 5...
May 1, 2019: Talanta
Liming Zhang, Liyun Guan, Zhuoxuan Lu, Miaosi Li, Jing Wu, Rong Cao, Junfei Tian
Patterned paper sensors such as letter- and barcode-shaped sensors have become a subject of growing interest due to the potential in information embedding and data interpretation, which brings a requirement on easily fabricating these paper-based analytical devices. The answer, in part, may lie in the influence of paper structure on the performance of paper-based analytical devices. After investigating the effect of physical properties of paper on precipitation and non-precipitation assays for detecting Fe(II), here we propose a simple and promising approach for barrier-free paper patterning...
May 1, 2019: Talanta
Richard P S de Campos, Darius G Rackus, Roger Shih, Chen Zhao, Xinyu Liu, Aaron R Wheeler
Digital microfluidics (DMF) is a platform that enables highly reconfigurable and automated fluidic operations using a generic device architecture. A unique hallmark of DMF is its "flexibility": a generic device design can be used and reused for many different, divergent fluidic operations. The flexibility of DMF is compromised when devices are permanently modified with embedded sensors. Here we introduce a solution to the "flexibility gap" between fluidic operations in digital microfluidics and embedded sensors: "plug-n-play DMF" (PnP-DMF)...
January 24, 2019: Analytical Chemistry
Chung-An Chen, Wen-Shin Yeh, Tsung-Ting Tsai, Yu-De Li, Chien-Fu Chen
In this study, we demonstrate a three-dimensional surface-modified origami-paper-based analytical device (3D-soPAD) for immunoassay applications. The platform enables the sequential steps of immunoassays to be easily performed using a folded, sliding paper design featuring multiple pre-stored reagents, allowing us to take advantage of the vertical diffusion of the analyte through the different paper layers. The cellulose substrate is composed of carboxymethyl cellulose modified with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide, which provide covalent bonding sites for bio-recognition molecules...
January 21, 2019: Lab on a Chip
Shuang Guo, Thomas Jacroux, Cornelius F Ivory, Lei Li, Wen-Ji Dong
The objective of this study is to explore an approach for analyzing negatively charged proteins using paper-based cationic isotachophoresis (ITP). The rationale of electrophoretic focusing the target protein with negative charges under unfavorable cationic ITP condition is to modify the electrophoretic mobility of the target protein through antigen-antibody immunobinding. Cationic ITP was performed on a paper-based analytical device that was fabricated using fiberglass paper. The paper matrix was modified with (3-aminopropyl)trimethoxysilane to minimize sample attraction to the surface for cationic ITP...
January 17, 2019: Electrophoresis
Mohammad Paknahad, Carmen Mcintosh, Mina Hoorfar
This paper studies the effect of channel coating hydrophobicity and analyte polarity on the gas detection capability of a microfluidic-based gas detector. Two detectors with two different channel surface coating combinations (resulting in different levels of hydrophobicity) are fabricated and tested against seven analytes with different polarities (methanol, ethanol, 1-propanol, 2-pentanol, acetone, pentane, and hexane). A feature extraction method is utilized to compare the discrimination capability of each of the fabricated detector...
January 17, 2019: Scientific Reports
Bruno Rodrigues de Oliveira, Caio Cesar Enside de Abreu, Marco Aparecido Queiroz Duarte, Jozue Vieira Filho
BACKGROUND AND OBJECTIVE: Premature ventricular contraction is associated to the risk of coronary heart disease, and its diagnosis depends on a long time heart monitoring. For this purpose, monitoring through Holter devices is often used and computational tools can provide essential assistance to specialists. This paper presents a new premature ventricular contraction recognition method based on a simplified set of features, extracted from geometric figures constructed over QRS complexes (Q, R and S waves)...
February 2019: Computer Methods and Programs in Biomedicine
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