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recombinant allergens

S Kespohl, Monika Raulf
Asthmatic patients are suspected of having a higher risk of mold sensitization. Thus, precise diagnosis of fungal sensitization is important. Mold allergen extracts are difficult to standardize, but component-resolved allergy diagnosis may be an alternative to replace extract-based tests. In this research, asthmatic and non-asthmatic subjects were studied for their sensitization to Aspergillus fumigatus (Asp f), Cladosporium herbarum (Cla h), Penicillium chrysogenum (Pen ch), Alternaria alternata (Alt a), and Aspergillus versicolor (Asp v)...
February 20, 2019: Advances in Experimental Medicine and Biology
M Vakili Moghaddam, M Fallahpour, M Mohammadi, F S Rasi Varaee, K Mokhtarian, M Khoshmirsafa, R Jafari, N Shirzad, R Falak
INTRODUCTION: Amaranthus retroflexus (Redroot Pigweed) is one of the main sources of allergenic pollens in temperate areas. Polcalcin is a well-known panallergen involved in cross-reactivity between different plants. The aim of this study was the molecular cloning and expression of polcalcin, as well as evaluating its IgE-reactivity with A. retroflexus sensitive patients' sera. METHODS: Allergenic extract was prepared from A. retroflexus pollen and the IgE-reactivity profile was determined by ELISA and immunoblotting using sera from twenty A...
February 12, 2019: Allergologia et Immunopathologia
Nunzio D'Agostino, Martina Buonanno, Joëlle Ayoub, Amalia Barone, Simona Maria Monti, Maria Manuela Rigano
Non-specific lipid transfer proteins (nsLTPs) are characterized by an eight-cysteine motif backbone that is stabilized by four disulphide bonds. The strong interest towards this protein family is mainly due to the fact that nsLTPs are involved in many biological processes and have been identified as major human allergens. Since tomato (Solanum lycopersicum L.) is one of the most consumed and allergenic vegetables, a full characterization of this family is needed. In this study, hidden Markov model profiles were used to identify nsLTPs within the tomato protein complement...
February 7, 2019: Scientific Reports
Kavita Reginald, Fook Tim Chew
Der p 2 is a major dust mite allergen and >80% of mite allergic individuals have specific IgE to this allergen. Although it is well characterized in terms of allergenicity, there is still some ambiguity in terms of its biological function. Three-dimensional structural analysis of Der p 2 and its close homologues indicate the presence of a hydrophobic cavity which can potentially bind to lipid molecules. In this study, we aimed to identify the potential ligand of Der p 2. Using a liposome pulldown assay, we show that recombinant Der p 2 binds to liposomes prepared with exogenous cholesterol in a dose dependent fashion...
February 7, 2019: Scientific Reports
Christian Seutter von Loetzen, Andreas Reuter, Jelena Spiric, Thomas Schulenborg, Iris Bellinghausen, Elke Völker, Lothar Vogel, Paul Rösch, Dirk Schiller
BACKGROUND: To date only limited information on structure, expression levels and IgE binding of Bet v 1 variants, which are simultaneously expressed in birch pollen, is available. OBJECTIVE: To analyze and compare structure and serum IgE/IgG binding of rBet v 1 variants to Bet v 1.0101. METHODS: Recombinant Bet v 1 variants were studied with sera of 20 subjects allergic to birch pollen. Folding, aggregation and solubility of the rBet v 1 variants was analyzed to attribute diverging IgE binding to either allergen structure or methodological features...
January 31, 2019: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Janire Orcajo, María Lavilla, Iñigo Martínez-de-Marañón
Immunochemical detection of food allergens is usually based on the use of polyclonal or monoclonal immunoglobulins G (IgG) antibodies. However, due to differences in epitopes recognition between IgG and IgE, an epitope modification during food processing can potentially alter allergenicity and detection in such different way. For that reason, the use of traditional immunological methods to anticipate or study the protein allergenicity is not recommended. The objective of this work was to develop a standardized competitive ELISA, based on recombinant IgE antibodies to β-lactoglobulin (β-lg), to reveal and to measure changes in β-lg immunoreactivity provoked by technological processes in foods containing this milk protein...
April 4, 2019: Analytica Chimica Acta
Ellen O Weinberg, Beatriz Ferran, Yuko Tsukahara, Michaela M S Hatch, Jingyan Han, Colin E Murdoch, Reiko Matsui
Interleukin (IL)-33 is an interleukin-1 like cytokine that enhances Th2 responses and mediates mucosal immunity and allergic inflammation but the mechanism regulating endogenous IL-33 production are still under investigation. In macrophages, lipopolysaccharide (LPS) administration resulted in marked induction of IL-33 mRNA that was blunted in macrophages from glutaredoxin-1 (Glrx) knockout mice and in RAW264.7 macrophages with Glrx knockdown by siRNA. Glutaredoxin-1 is a small cytosolic thioltransferase that controls a reversible protein thiol modification, S-glutationylation (protein-GSH adducts), thereby regulating redox signaling...
2019: PloS One
Anita Patel, Cathy F Curtis, Rosario Cerundolo
Recent studies from North America and continental Europe have reported Zen 1 as a major allergen in atopic dogs, and Der f 2 as a minor allergen. In contrast, Der f 2 is considered a major allergen in Japan. In this study, allergen-specific IgE against Der f 2, Zen 1 and crude Dermatophagoides farinae (DF) was determined using ELISA assays in English atopic dogs. Serum samples were obtained from 59 dogs with non-seasonal atopic dermatitis. ELISA assays using horseradish peroxidase-labelled anti-dog IgE monoclonal antibody (Bethyl; A40-125P) and recombinant Der f 2 (Zenoaq), natural Zen 1 (Zenoaq) and DF extract (Greer Laboratories; North Carolina) were performed by Zenoaq, Fukushima, Japan...
January 23, 2019: Veterinary Record
Rui-Qi Wang, Yu-Jie Wang, Zhi-Qiang Xu, Yan-Jun Zhou, Meng-Da Cao, Wei Zhu, Jin-Lyu Sun, Ji-Fu Wei
Dogs are a major source of indoor allergens. However, the prevalence of dog allergies in China remains unclear, especially in children. In the present study, Can f 7, a canine allergen belonging to the Niemann pick type C2 protein family, was selected to study its sensitization rate in Chinese children with dog allergies. The Can f 7 gene was subcloned into a pET‑28a vector and expressed in Escherichia coli BL21 (DE3) cells. Recombinant Can f 7 was purified by nickel affinity chromatography, identified by SDS‑PAGE electrophoresis, and had its allergenicity assessed by western blot, ELISA and basophil activation tests...
January 15, 2019: International Journal of Molecular Medicine
Rongchang Chen, Qinglin Zhang, Shuyu Chen, Haixiong Tang, Peikai Huang, Shushan Wei, Lihong Yao
Steroid insensitivity constitutes the major problem for asthma management. Toluene diisocyanate (TDI) is one of the leading allergens of asthma that induces both Th2 and Th17 responses and is often associated with poor responsiveness to steroid treatment in the clinic. We sought to evaluate the effects of inhaled and systemic steroids on a TDI-induced asthma model and to find how IL-17A and IL-17F function in this model. BALB/c mice were exposed to TDI for generating an asthma model, and were treated with inhaled fluticasone propionate (FP), systemic prednisone (Pred), anti-IL-17A, anti-IL-17F, recombinant IL-17A or IL17F...
January 17, 2019: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Chong Joo Chan, Timmy Richardo, Renee Lay Hong Lim
Peanut allergy is a hypersensitivity reaction with symptoms varying from mild to severe anaphylaxis, tends to be lifelong and very few are able to outgrow this allergy. The prevalence of peanut allergy is highest among the Western countries and over the past decade, a 3.5 fold increase in prevalence of peanut allergy was reported among children in the United States. Increasing prevalence has also been observed among the Asian countries. As with other food allergies, peanut allergy reduces quality of life for the affected individuals and the social and economy burden of healthcare for peanut allergy is substantial...
January 13, 2019: International Reviews of Immunology
Yutaka Ueda, Kazuyuki Nakagome, Takehito Kobayashi, Toru Noguchi, Tomoyuki Soma, Katsuyo Ohashi-Doi, Kenichi Tokuyama, Makoto Nagata
BACKGROUND: Even in subjects who are not sensitized to house dust mite (HDM), allergic symptoms can be aggravated by exposure to dust, suggesting that innate immune responses may be involved in these processes. Since eosinophils express pattern recognition receptors, HDM may directly upregulate eosinophil functions through these re ceptors. The objective of this study was to examine whether Dermatophagoides farinae (Df), a representative HDM, or Der f 1, a major allergen of Df, modifies the effector functions of eosinophils...
January 10, 2019: International Archives of Allergy and Immunology
S Lahiani, M E Dumez, I Bitam, M Galleni
Der p 5 is one of the important house dust mite allergens in Algeria; this allergen is frequently recognized by patients with allergic asthma. However, there is no information on its IgG-binding epitopes. In the present study, rabbits were immunized with recombinant Der p 5 allergen, and serum samples were obtained. Recognition of linear IgG epitopes of Der p 5 was determined using synthesized peptides derived from the allergen sequence. The results showed that serum from immunized rabbits recognized three linear epitopes from Der p 5 (28 EDKKHDYQNEFDFLLMERIHEQIK43 ), (37 IHEQIKKGELALFYLQEQ55 ) and (92 LMQRKDLDIFEQYNLEMAKKS112 )...
January 2019: New Microbes and New Infections
Maciej Kochanowski, Miguel González-Muñoz, María Ángeles Gómez-Morales, Bruno Gottstein, Joanna Dąbrowska, Mirosław Różycki, Tomasz Cencek, Norbert Müller, Ghalia Boubaker
Serodiagnosis of human anisakidosis is presently hampered by the current lack of standardised serological assays that allow sensitive and specific detection of Anisakidae-specific antibodies in human patients. In the present study, we comparatively evaluated the diagnostic value (by IgG-ELISA) of excretory-secretory antigens (ESAgs) of Anisakis simplex, Pseudoterranova decipiens and Contracaecum osculatum, representing the most frequently found genera responsible for human infection. In addition, we tested also a mix of the three ES preparations (Mix-ESAgs) as well as two recombinant allergens of A...
December 22, 2018: Experimental Parasitology
Pia Gattinger, Irene Mittermann, Christian Lupinek, Gerhard Hofer, Walter Keller, Urska Bidovec Stojkovic, Peter Korosec, Christine Koessler, Natalija Novak, Rudolf Valenta
BACKGROUND: N-linked glycans present in venoms, pollen and mites are recognized by IgE antibodies from >20% of allergic patients but have low or no allergenic activity. OBJECTIVES: To engineer recombinant glycoproteins resembling carbohydrate-specific IgE epitopes from venoms, pollen and mites which can discriminate carbohydrate-specific IgE from allergenic, peptide-specific IgE. METHODS: One or two N-glycosylation sites were engineered into the N-terminus of the non-allergenic protein horse heart myoglobin (HHM) using synthetic gene technology...
December 20, 2018: EBioMedicine
Liudmila Slogotskaya, Elena Bogorodskaya, Diana Ivanova, Tatiana Sevostyanova
BACKGROUND: A group of Russian scientists has developed Diaskintest, which comprises Mycobacterium tuberculosis-specific recombinant proteins CFP10-ESAT6, for skin testing (0.2 μg/0.1 ml). STUDY PURPOSE: To evaluate the comparative sensitivity of TST with 2 TU PPD-L and a skin test with tuberculous recombinant allergen (Diaskintest) containing the ESAT6-CFP10 protein in children and adolescents with newly diagnosed active tuberculosis during mass screening in the primary medical service in Moscow...
2018: PloS One
Chang-Ching Yeh, Keh-Gong Wu, Peng-Hui Wang
There is an increase in prevalence and financial burden of childhood atopic disorders in recent years. Understanding allergic conditions of pregnant women is important for developing strategies for prevention and management of allergy-related diseases. However, little is currently known about the atopic conditions in pregnant women.The sera from 46 pregnant women were analyzed for allergen-specific IgE antibodies using the Optigen assay and SDS-PAGE immunoblot analysis.Results from the Optigen assay showed that 20 (43%) of the 46 serum samples analyzed demonstrated IgE reactivity against mite p (Dermatophagoides pteronyssinus) (95%), mite f (D farinae) (95%), house dust (60%), cat (25%), shrimp (20%), crab (15%), cockroach (10%), dog (5%), latex (5%), willow black (5%), and timothy grass (5%)...
December 2018: Medicine (Baltimore)
Fatma Besbes, Katrin Franz-Oberdorf, Wilfried Schwab
Abiotic and biotic stress situations cause the upregulation of the transcription of a number of plant defence genes. They code for so-called pathogenesis-related (PR) proteins such as PR proteins of class-10 (PR-10), whose biological functions are still unclear. PR10 proteins are members of the Bet v 1 (major birch pollen allergen) superfamily including related proteins from the cultivated strawberry Fragaria × ananassa (Fra a 1 proteins). Here, we analyzed the expression of 21 Fra a 1 genes in different tissues of the strawberry plant by quantitative real-time PCR...
December 7, 2018: Journal of Plant Physiology
So Myung Song, Shin Ae Kang, Hye Kyung Park, Dong Hee Kim, So Young Park, Se Bok Jang, Hak Sun Yu
BACKGROUND: In previous studies, we suggested that Acanthamoeba is a new aero-allergen and that patients who showed positive results for the skin-prick test response to Acanthamoeba cross-reacted with several pollen allergens. Additionally, patients with common antibodies reacted to the 13-15 kDa Acanthamoeba unknown allergen. OBJECTIVE: We examined whether profilin of Acanthamoeba is a human airway allergic agent because of its molecular weight. METHODS: We expressed recombinant Ac-PF (rAc-PF) protein using an Escherichia coli expression system and evaluated whether Ac-PF is an airway allergic agent using an allergic airway inflammation animal model...
December 17, 2018: PLoS Neglected Tropical Diseases
R Manjunatha Kini, Sachdev S Sidhu, Andreas Hougaard Laustsen
Snakebite envenoming is a neglected tropical disease that each year claims the lives of 80,000⁻140,000 victims worldwide. The only effective treatment against envenoming involves intravenous administration of antivenoms that comprise antibodies that have been isolated from the plasma of immunized animals, typically horses. The drawbacks of such conventional horse-derived antivenoms include their propensity for causing allergenic adverse reactions due to their heterologous and foreign nature, an inability to effectively neutralize toxins in distal tissue, a low content of toxin-neutralizing antibodies, and a complex manufacturing process that is dependent on husbandry and procurement of snake venoms...
December 13, 2018: Toxins
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