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FISH MYC

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https://read.qxmd.com/read/30737994/stratification-by-myc-expression-has-prognostic-impact-in-myc-bcl2-translocated-b-cell-lymphoma-identifies-a-subgroup-of-patients-with-poor-outcome
#1
Mette Ølgod Pedersen, Anne Ortved Gang, Erik Clasen Linde, Marie Fredslund Breinholt, Helle Knudsen, Signe Ledou Nielsen, Tim Svenstrup Poulsen, Tobias Wirenfeldt Klausen, Estrid Høgdall, Peter Nørgaard
OBJECTIVE: In patients with Large B-cell Lymphoma (LBCL) according to WHO the prognostic significance of MYC translocation is still not sufficiently clarified. We therefore aimed to investigate whether prognostication could be improved in patients with MYC translocation positive LBCL by additional stratification according to MYC and BCL2 protein expression levels or MYC translocation partner gene as well as concurrent BCL2 and/or BCL6 translocation (DH). METHODS: From an unselected consecutive cohort of >600 patients with LBCL investigated with fluorescent in situ hybridization (FISH) 64 patients were diagnosed with MYC translocation positive LBCL and included in the study...
February 9, 2019: European Journal of Haematology
https://read.qxmd.com/read/30734478/bright-cd38-expression-by-flow-cytometric-analysis-is-a-biomarker-for-double-triple-hit-lymphomas-with-a-moderate-sensitivity-and-high-specificity
#2
Abdullah Alsuwaidan, Elaina Pirruccello, Jesse Jaso, Prasad Koduru, Rolando Garcia, JoEllen Krueger, Meggie Doucet, Rahman Chaudhry, Franklin Fuda, Weina Chen
BACKGROUND: High-grade B-cell lymphomas (HGBCL) with MYC and BCL2 or/and BCL6 rearrangements (R), so-called double/triple-hit lymphomas (DH/THL), are uncommon, clinically aggressive lymphomas that require a prompt diagnosis. We aim to identify flow cytometric immunophenotypic (IP) features of DH/THL that may aid in triaging these cases followed by a timely confirmatory cytogenetic study. METHODS: We compared the IP features of 43 cases of DH/THL to those of 55 cases of single-hit lymphoma (SHL) and 59 cases of diffuse large B-cell lymphoma (DLBCL) without MYC-R (MYCneg DLBCL)...
February 7, 2019: Cytometry. Part B, Clinical Cytometry
https://read.qxmd.com/read/30701920/structure-and-significance-of-cytogenetic-abnormalities-in-adult-patients-with-ph-negative-acute-lymphoblastic-leukemia
#3
I S Piskunova, T N Obukhova, E N Parovichnikova, S M Kulikov, V V Troitskaya, O A Gavrilina, V G Savchenko
AIM: To evaluate occurrence, variety, structural peculiarities and prognostic meaning of cytogenetic abnormalities in adult patients with Ph-negative acute lymphoblastic leukemia (ALL) receiving therapy according to ALL-2009 protocol. MATERIALS AND METHODS: The study included 115 adult patients with firstly diagnosed Ph-negative ALL: 58 male and 57 female aged from 15 to 61 years (mean age 26.5 years), who underwent treatment from September 2009 to September 2015 in National Medical Research Center for Hematology MH RF (n=101) and in hematology departments of regional hospitals (n=14)...
August 17, 2018: Terapevticheskiĭ Arkhiv
https://read.qxmd.com/read/30675277/genomic-amplification-of-hpv-h-terc-and-c-myc-in-liquid-based-cytological-specimens-for-screening-of-cervical-intraepithelial-neoplasia-and-cancer
#4
Wenting Ji, Weihua Lou, Zubei Hong, Lihua Qiu, Wen Di
Cervical cancer is one of the most prevalent female cancer types in developing countries. ThinPrep cytological test (TCT) and human papillomavirus (HPV) detection are canonical screening methods for cervical cancer currently. However, there are limitations to these techniques. The aim of the present study was to identify efficient and practical methods for the screening of cervical intraepithelial neoplasia (CIN) and carcinoma. Residual PreservCyt specimens were obtained from 1,000 women who were admitted between August 2013 and December 2015...
February 2019: Oncology Letters
https://read.qxmd.com/read/30636079/melanoma-types-by-in-vivo-reflectance-confocal-microscopy-correlated-with-protein-and-molecular-genetic-alterations-a-pilot-study
#5
F Beretti, L Bertoni, F Farnetani, C Pellegrini, G Gorelli, A M Cesinaro, L Reggiani Bonetti, L Di Nardo, S Kaleci, J Chester, C Longo, D Massi, M C Fargnoli, G Pellacani
Cutaneous melanoma (CM) is one of the most prevalent skin cancers, which lacks both a prognostic marker and a specific and lasting treatment, due to the complexity of the disease and heterogeneity of patients. Reflectance confocal microscopy (RCM) in vivo analysis is a versatile approach offering immediate morphological information, enabling the identification of four primary cutaneous RCM CM types. Whether RCM CM types are associated with a specific protein and molecular-genetic profiles at the tissue level, remains unclear...
January 12, 2019: Experimental Dermatology
https://read.qxmd.com/read/30611450/large-chromosomal-rearrangements-yield-biomarkers-to-distinguish-low-risk-from-intermediate-and-high-risk-prostate-cancer
#6
George Vasmatzis, Farhad Kosari, Stephen J Murphy, Simone Terra, Irina V Kovtun, Faye R Harris, Shabnam Zarei, James B Smadbeck, Sarah H Johnson, Athanasios G Gaitatzes, Terry M Therneau, Laureano J Rangel, Ryan A Knudson, Patricia Greipp, William R Sukov, Darlene L Knutson, Sara M Kloft-Nelson, R Jeffrey Karnes, John C Cheville
OBJECTIVE: To test the hypothesis that chromosomal rearrangements (CRs) can distinguish low risk of progression (LRP) from intermediate and high risk of progression (IHRP) to prostate cancer (PCa) and if these CRs have the potential to identify men with LRP on needle biopsy that harbor IHRP PCa in the prostate gland. PATIENTS AND METHODS: Mate pair sequencing of amplified DNA from pure populations of Gleason patterns in 154 frozen specimens from 126 patients obtained between August 14, 2001, and July 15, 2011, was used to detect CRs including abnormal junctions and copy number variations...
January 2019: Mayo Clinic Proceedings
https://read.qxmd.com/read/30576868/t-3-8-q26-2-q24-often-leads-to-mecom-myc-rearrangement-and-is-commonly-associated-with-therapy-related-myeloid-neoplasms-and-or-disease-progression
#7
Guilin Tang, Shimin Hu, Sa A Wang, Wei Xie, Pei Lin, Jie Xu, Gokce Toruner, Ming Zhao, Jun Gu, Madison Doty, Shaoying Li, L Jeffrey Medeiros, Zhenya Tang
t(3;8)(q26.2;q24) is a rare recurrent cytogenetic abnormality that is associated with myeloid neoplasms. We report 20 patients with t(3;8)(q26.2,q24): eight had therapy-related acute myeloid leukemia (AML), three therapy-related myelodysplastic syndrome, four blast phase of chronic myeloid leukemia, one relapsed AML, one AML transformed from chronic myelomonocytic leukemia, one blast phase of an unclassifiable myeloproliferative neoplasm, one de novo myelodysplastic syndrome, and one de novo AML. Nineteen patients presented with cytopenia...
December 18, 2018: Journal of Molecular Diagnostics: JMD
https://read.qxmd.com/read/30553470/primary-extranodal-diffuse-large-b-cell-lymphomas-many-sites-many-entities-clinico-pathological-immunohistochemical-and-cytogenetic-study-of-106-cases
#8
Francesca Magnoli, Barbara Bernasconi, Lisa Vivian, Ilaria Proserpio, Graziella Pinotti, Leonardo Campiotti, Luca Mazzucchelli, Fausto Sessa, Maria Grazia Tibiletti, Silvia Uccella
We analyzed the clinicopathological, immunohistochemical and cytogenetic features of 106 extranodal (EN) diffuse large B-cell lymphomas (DLBCLs) from stomach (34 cases), intestine (10), cervico-cephalic region (11), central nervous system (13), testes (21), skin (8), and miscellaneous sites (9). Hans' algorithm and the immunohistochemical double expressor score (DES) for MYC and BCL2 were applied to all cases. A subset of fifty-eight cases were analyzed with fluorescent in situ hybridization (FISH) with specific break apart probes for BCL6, MYC, BCL2, CCND1, BCL10 and MALT1 genes...
December 2018: Cancer Genetics
https://read.qxmd.com/read/30523057/false-negative-rates-for-myc-fish-probes-in-b-cell-neoplasms
#9
Rebecca L King, Ellen D McPhail, Reid G Meyer, George Vasmatzis, Kathryn Pearce, James B Smadbeck, Rhett P Ketterling, Stephanie A Smoley, Patricia T Greipp, Nicole L Hoppman, Jess F Peterson, Linda B Baughn
No abstract text is available yet for this article.
December 6, 2018: Haematologica
https://read.qxmd.com/read/30522172/-diagnostic-and-therapeutic-values-of-interphase-fluorescence-in-situ-hybridization-in-b-cell-lymphomas-a-clinicopathologic-analysis-of-604-cases
#10
M Chen, J L Yang, S Zhao, W P Liu, G D Li, Y X Ye, J Q Yan, W Y Zhang
Objective: To investigate the feasibility and value of interphase fluorescence in situ hybridization (FISH) in the pathological diagnosis, differential diagnosis and therapeutic assessment of B-cell lymphomas. Methods: The cohort included 604 cases of B-cell lymphoma which were collected at West China Hospital from May 2010 to December 2016.And all were subjected to interphase FISH using 11 break apart or fusion probes (MYC, bcl-2, bcl-6, IRF4, MYC/IgH, bcl-2/IgH, CCND1/IgH, IgH, API2/MALT1, p53/ATM, and D13S319/CEP12)...
December 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://read.qxmd.com/read/30504422/zebrafish-ndrg1a-negatively-regulates-ifn-induction-by-promoting-the-degradation-of-irf7
#11
Long-Feng Lu, Shun Li, Zhao-Xi Wang, Shu-Bo Liu, Dan-Dan Chen, Yong-An Zhang
Viral infection activates the transcription factor IFN regulatory factor 7 (IRF7), which plays a critical role in the induction of IFNs and innate antiviral immune response. How virus-induced IFN signaling is controlled in fish is not fully understood. In this study, we demonstrate that N-myc downstream-regulated gene 1a (NDRG1a) in zebrafish plays a role as a negative regulator for virus-triggered IFN induction. First, the activation of the IFN promoter stimulated by the polyinosinic-polycytidylic acid or spring viremia of carp virus was decreased by the overexpression of NDRG1a...
November 30, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://read.qxmd.com/read/30457458/radiotherapy-for-early-stage-diffuse-large-b-cell-lymphoma-with-or-without-double-or-triple-hit-genetic-alterations
#12
George Daniel Grass, Matthew N Mills, Kamran A Ahmed, Casey L Liveringhouse, Michael J Montejo, Timothy J Robinson, Julio C Chavez, Louis B Harrison, Sungjune Kim
We investigated whether adding radiation (RT) to systemic therapy improved outcomes in early stage diffuse large B-cell lymphoma (DLBCL) patients with or without double- or triple-hit lymphoma (DHL/THL) biology. This analysis included 183 patients profiled with fluorescent in situ hybridization (FISH) for alterations in MYC, BLC2, and/or BCL6. A total of 146 (80%) were non-DHL/THL, 27 (15%) were DHL, and 10 (6%) were THL. Systemic therapy without RT resulted in inferior freedom from relapse (FFR) (HR: 2.28; 95% CI, 1...
November 20, 2018: Leukemia & Lymphoma
https://read.qxmd.com/read/30448222/immunohistochemical-pattern-of-c-myc-protein-judged-as-weak-by-a-new-notation-correlates-with-myc-gene-non-translocation-in-large-b-cell-lymphoma
#13
Fumiyoshi Fujishima, Hiroki Katsushima, Noriko Fukuhara, Sachiko Konosu-Fukaya, Yasuhiro Nakamura, Hajime Usubuchi, Satoko Sato, Yasunori Ota, Akiko Yashima-Abo, Tomohiro Nakamura, Naoki Nakaya, Hideo Harigae, Hironobu Sasano, Ryo Ichinohasama
Immunohistochemistry is not only the most important tool for pathologists to establish a final diagnosis but it can also inform decisions regarding optimal treatment methods. However, there is no universal standard notation for expressing immunohistochemical findings. For a diagnosis of malignant lymphoma, it is important to confirm the presence or absence of MYC translocation and communicate these results to a clinical audience. However, the criteria for selecting cases for fluorescence in situ hybridization (FISH) analysis to confirm MYC translocation are ill-defined...
November 15, 2018: Human Pathology
https://read.qxmd.com/read/30385370/amplicons-in-breast-cancers-analyzed-by-multiplex-ligation-dependent-probe-amplification-and-fluorescence-in-situ-hybridization
#14
Akishi Ooi, Masafumi Inokuchi, Shin-Ichi Horike, Hiroko Kawashima, Satoko Ishikawa, Hiroko Ikeda, Ritsuko Nakamura, Takeru Oyama, Yoh Dobash
Gene amplification is a common event in breast cancer, and identifies actual and potential targets of molecular therapy. The aim of the present study was to determine the amplification status of 22 genes that are reportedly frequently amplified in breast cancers. An archive of 322 formalin-fixed and paraffin-embedded invasive breast cancer tissues were screened by multiple ligation-dependent probe amplification (MLPA) and a total of 906 gene loci judged as 'gain' or 'amplified' was further confirmed to have been amplified based on fluorescence in situ hybridization (FISH)...
October 29, 2018: Human Pathology
https://read.qxmd.com/read/30382301/prognostic-analysis-of-interim-18-f-fdg-pet-ct-in-patients-with-diffuse-large-b-cell-lymphoma-after-one-cycle-versus-two-cycles-of-chemotherapy
#15
Ling Yuan, Michael C Kreissl, Liping Su, Zhifang Wu, Marcus Hacker, Jianzhong Liu, Xi Zhang, Yunfeng Bo, Hongyu Zhang, Xiang Li, Sijin Li
OBJECTIVES: 18 F-fluorodeoxyglucose (FDG) positron emission tomography (PET) is routinely used in diffuse large B cell lymphoma (DLBCL) for staging, assessment of remission and recurrence, and estimation of therapeutic efficacy. In this study, we aimed to assess the role of an early interim PET/computed tomography (CT) in the evaluation of response in DLBCL. METHODS: Sixty primary DLBCL patients (31 females) were analyzed. Baseline and follow-up 18 F-FDG PET/CT was performed in patients after one cycle (n = 30) and two cycles (n = 30) of chemotherapy...
October 31, 2018: European Journal of Nuclear Medicine and Molecular Imaging
https://read.qxmd.com/read/30351860/clinicopathological-and-fluorescence-in-situ-hibridisation-analysis-of-primary-testicular-diffuse-large-b-cell-lymphoma-a-single-centre-case-series
#16
Maja Perunicic Jovanovic, Biljana Mihaljevic, Petar Jovanovic, Jelena Jelicic, Vesna Cemerikic Martinovic, Jelica Jovanović, Marija Dencic Fekete, Milica Čekerevac, Nebojša Bojanić
Primary testicular diffuse large B-cell lymphoma (PT-DLBCL) represents a rare and aggressive extranodal non-Hodgkin's lymphoma (NHL) with some specific features that differ from other NHLs. Formalin fixed, paraffin wax embedded (FFPE) samples of 21 PT-DLBCLs and 30 comparative patients with DLBCL were analysed. All PT-DLBCL patients were treated with rituximab-containing regimens, intrathecal prophylaxis (10 patients), and irradiation of the contralateral testis (9 patients). FFPE samples were additionally analysed by immunohistochemistry (Bcl-2, c-Myc protein expression) and fluorescence in situ hybridisation (FISH) (BCL2 and MYC)...
2018: Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists
https://read.qxmd.com/read/30348671/high-resolution-architecture-and-partner-genes-of-myc-rearrangements-in-lymphoma-with-dlbcl-morphology
#17
Lauren C Chong, Susana Ben-Neriah, Graham W Slack, Ciara Freeman, Daisuke Ennishi, Anja Mottok, Brett Collinge, Pau Abrisqueta, Pedro Farinha, Merrill Boyle, Barbara Meissner, Robert Kridel, Alina S Gerrie, Diego Villa, Kerry J Savage, Laurie H Sehn, Reiner Siebert, Ryan D Morin, Randy D Gascoyne, Marco A Marra, Joseph M Connors, Andrew J Mungall, Christian Steidl, David W Scott
Genomic rearrangements in the MYC locus occur in ∼12% of lymphomas with diffuse large B-cell lymphoma (DLBCL) morphology and are associated with inferior outcome. Previous studies exploring MYC rearrangements have primarily used fluorescence in situ hybridization (FISH) assays to characterize break-apart status but have rarely examined breakpoint location, and in some cases have not examined partner identity. We performed targeted sequencing of MYC , BCL2 , BCL6 , and the immunoglobulin ( IG ) loci in 112 tumors with DLBCL morphology harboring MYC rearrangement...
October 23, 2018: Blood Advances
https://read.qxmd.com/read/30343007/chronic-exposure-to-the-ionic-liquid-c-8-mim-br-induces-inflammation-in-silver-carp-spleen-involvement-of-oxidative-stress-mediated-p38mapk-nf-%C3%AE%C2%BAb-signalling-and-micrornas
#18
Junguo Ma, Xi Chen, Guangyuan Xin, Xiaoyu Li
The present study aimed to determine the chronic toxicity of 1-methyl-3-octylimidazolium bromide ([C8 mim]Br) on the silver carp to further reveal the toxicological mechanisms of ionic liquids. Chronic exposure of silver carp to [C8 mim]Br at concentrations of 1.095 and 4.380 mg/L for 60 d was conducted under laboratory conditions. The results revealed that chronic exposure to [C8 mim]Br inhibited the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and reduced glutathione (GSH) levels while markedly increasing malondialdehyde (MDA) and protein carbonyl (PC) levels in fish spleen, indicating that [C8 mim]Br treatment induced oxidative stress...
October 19, 2018: Fish & Shellfish Immunology
https://read.qxmd.com/read/30306208/myc-bcl2-and-bcl6-rearrangements-in-primary-central-nervous-system-lymphoma-of-large-b-cell-type
#19
Anahita Nosrati, Ahmad Monabati, Alireza Sadeghipour, Fatemeh Radmanesh, Akbar Safaei, Sajjadeh Movahedinia
Primary central nervous system lymphoma (PCNSL) is a rare specific subtype of non-Hodgkin lymphoma limited to the brain, leptomeninges, spinal cord, or eyes without any systemic presentation and relapse which mostly takes place in CNS. In more than 95% of patients, it is of diffuse large B cell lymphoma (DLBCL) type. Categorizing PCNSL to germinal center cell like or activated B cell like, as we usually do for DLBCL NOS, may not be applicable for predicting outcome. Possible prognostic significance of MYC, BCL2, and/or BCL6 rearrangements may be important given what we know about their impact in systemic DLBCL, but we have limited knowledge about the status of double or triple hit molecular changes in PCNSL...
October 11, 2018: Annals of Hematology
https://read.qxmd.com/read/30290287/myc-amplification-as-a-potential-mechanism-of-primary-resistance-to-crizotinib-in-alk-rearranged-non-small-cell-lung-cancer-a-brief-report
#20
Karim Rihawi, Roberta Alfieri, Michelangelo Fiorentino, Francesca Fontana, Elisa Capizzi, Andrea Cavazzoni, Mario Terracciano, Silvia La Monica, Alberto Ferrarini, Genny Buson, Pier Giorgio Petronini, Andrea Ardizzoni
INTRODUCTION: Translocations of the anaplastic lymphoma kinase (ALK) can be effectively targeted in advanced non-small cell lung cancer by ALK-TKI inhibitors including Crizotinib. However, the development of acquired resistance often limits the duration of these therapies. While several mechanisms of secondary resistance have been already identified, little is known about molecular determinants of primary resistance. In our brief report we investigated the tumor molecular profile of a patient who failed to respond to Crizotinib...
October 2, 2018: Translational Oncology
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