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Human ubilical vein endothelial cells

Antonio Campos Muñoz
Construction of human blood vessels by tissue engineering is strongly dependent of the use of viable and fully functional cultured endothelial cells (ECs). In this work, we have determined in a preliminary study both cell viability and PGI2 activity in primary cell cultures of human ubilical vein ECs, to identify the specific cell passage that is more appropriate to be used in tissue engineering protocols. Cell viability was determined by quantification of the intracellular cocnentration of several ions by electron probe X-ray microanalysis, whereas PGI2 release was quantified by radioimmunoassay...
2008: Anales de la Real Academia Nacional de Medicina
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