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Mir-22 and cardiomyocytes

Peng Wu, Lingchen Kong, Jianzhong Li
The aim of the present study was to investigate the role of microRNA (miR)-494-3p in myocardial injury in patients with septic shock and the underlying mechanism. A total of 22 patients with sepsis and 17 patients with septic shock were included in the present study. In addition, 20 healthy subjects were recruited as the control group. Peripheral blood was collected from all subjects and a rat cardiomyocyte model of myocardial injury was constructed. Reverse transcription-quantitative polymerase chain reaction was used to measure miR-494-3p expression, while cell counting kit-8 assays were performed to assess cell proliferation...
March 2019: Experimental and Therapeutic Medicine
Jinwen Su, Ming Fang, Bei Tian, Jun Luo, Can Jin, Xuejun Wang, Zhongping Ning, Xinming Li
Heart failure (HF) induced by ischemia myocardial infarction (MI) is one of the major causes of morbidity and mortality all around the world. Atorvastatin, a hydroxymethylglutaryl coenzyme A reductase inhibitor, has been demonstrated to benefit patients with ischemic or non-ischemic-induced HF, but the mechanism is still poorly understood. Increasing evidence indicates that lncRNAs play important role in variety of human disease. However, the role and underlying molecular mechanisms remain largely unclear. In our work, we applied 0...
November 27, 2018: Acta Biochimica et Biophysica Sinica
Asif Nadeem, Muhammad Rizwan Ashraf, Maryam Javed, Tanveer Hussain, Muhammad Salman Tariq, Masroor Ellahi Babar
In 1993 miRNAs were discovered during a research on Caenorhabditis elegans conducted by Victor Ambros and Gary Ruvkun. The gene lin-4 that played important role in development in C. elgans was observed not encoding any protein but a very small RNA molecule of just 22 nucleotides. Main objective of this review is to highlight the significance of miRNAs in regulating the expression of many genes, which are either directly or indirectly involved in many diseases. One of the major causes of illness and death in developed countries of the world is cardiovascular disease...
September 2018: Pakistan Journal of Pharmaceutical Sciences
Hui Pan, Linlin Zhu
BACKGROUND: The cardioprotective role of Angelica sinensis has been proven in previous studies. However, the effects of Angelica sinensis polysaccharide (ASP, major bioactive component of Angelica sinensis) on myocardial infarction (MI) remain unclear. This study aimed to investigate the effects of ASP on hypoxia-induced H9c2 cell injury as well as the underlying mechanisms. METHODS: We constructed in vitro hypoxic model to mimic MI. Cell viability, proliferation and apoptosis were respectively measured by using CCK-8 assay, Western blot analysis, and flow cytometry assay/Western blot analysis, to evaluate cell injury after treatments...
October 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Xiang Zhou, Wei Zhang, Mengchao Jin, Jianchang Chen, Weiting Xu, Xiangqing Kong
We previously established a rat model of diabetic cardiomyopathy (DCM) and found that the expression of long non-coding RNA myocardial infarction-associated transcript (MIAT) was significantly upregulated. The present study was aimed to determine the pathologic role of MIAT in the development of DCM. MIAT knockdown was found to reduce cardiomyocyte apoptosis and improve left ventricular function in diabetic rats. High glucose could increase MIAT expression and induce apoptosis in cultured neonatal cardiomyocytes...
July 13, 2017: Cell Death & Disease
Shashi Kumar Gupta, Ariana Foinquinos, Sabrina Thum, Janet Remke, Karina Zimmer, Christophe Bauters, Pascal de Groote, Reinier A Boon, Leon J de Windt, Sebastian Preissl, Lutz Hein, Sandor Batkai, Florence Pinet, Thomas Thum
BACKGROUND: Aging populations show higher incidences of myocardial infarction (MI) and heart failure (HF). Cardiac remodeling post-MI leads to progressive impaired cardiac function caused by a disarray of several processes including derailed autophagy. Microribonucleic acids (miRNAs) are known to be key players in cardiovascular disease but their involvement in cardiac autophagy and aging is not well understood. OBJECTIVES: This study sought to identify new miRNA candidates that regulate cardiac autophagy and aging...
October 4, 2016: Journal of the American College of Cardiology
Guoran Li, Guokun Wang, Liangliang Ma, Jun Guo, Jingwen Song, Liping Ma, Xianxian Zhao
microRNAs (miRNAs) are short noncoding RNAs that function in RNA silencing and post-transcriptional regulation of gene expression. They play critical regulatory roles in many cardiovascular diseases, including ischemia-induced cardiac injury. Here, we report microRNA-22, highly expressed in the heart, can protect cardiomyocytes from starvation-induced injury through promoting autophagy and inhibiting apoptosis. Quantitative real-time PCR (qPCR) demonstrated that the expression of miR-22 in starvation-treated neonatal rat cardiomyocytes (NRCMs) was markedly down-regulated...
September 23, 2016: Biochemical and Biophysical Research Communications
Jian-Kui Du, Bin-Hai Cong, Qing Yu, He Wang, Long Wang, Chang-Nan Wang, Xiao-Lu Tang, Jian-Qiang Lu, Xiao-Yan Zhu, Xin Ni
Mitochondrial oxidative damage is critically involved in cardiac ischemia reperfusion (I/R) injury. MicroRNA-22 (miR-22) has been predicted to potentially target sirtuin-1 (Sirt1) and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC1α), both of which are known to provide protection against mitochondrial oxidative injury. The present study aims to investigate whether miR-22 is involved in the regulation of cardiac I/R injury by regulation of mitochondrial function. We found that miR-22 level was significantly increased in rat hearts subjected to I/R injury, as compared with the sham group...
July 2016: Free Radical Biology & Medicine
Jian Yang, Lihua Chen, Jiawang Ding, Jing Zhang, Zhixing Fan, Chaojun Yang, Qinqin Yu, Jun Yang
MicroRNAs (miRNAs) are implicated in the regulation of pathological and physiological processes in myocardial ischemia/reperfusion (MI/R). Recent studies have revealed that miR-22 might provide a potential cardioprotective effect on ischemic heart disease. However, the mechanism by which miR-22 prevents MI/R is still not fully clear. Here, we investigated the role of miR-22 in hypoxia/reoxygenation (H/R)-induced cardiomyocyte injury. MI/R was simulated in neonatal rat cardiomyocytes with 2h hypoxia followed by 4h reoxygenation...
March 15, 2016: Gene
Lihua Wang, Guixian Song, Ming Liu, Bin Chen, Yumei Chen, Yahui Shen, Jingai Zhu, Xiaoyu Zhou
Our previous study reported that microRNA-375 (miR-375) is significantly upregulated in ventricular septal myocardial tissues from 22‑week‑old fetuses with ventricular septal defect as compared with normal controls. In the present study, the specific effects of miR‑375 on P19 cell differentiation into cardiomyocyte‑like cells were investigated. Stable P19 cell lines overexpressing miR‑375 or containing empty vector were established, which could be efficiently induced into cardiomyocyte‑like cells in the presence of dimethyl sulfoxide in vitro...
January 2016: International Journal of Molecular Medicine
Estefania Lozano-Velasco, Jennifer Galiano-Torres, Alvaro Jodar-Garcia, Amelia E Aranega, Diego Franco
MicroRNAs are noncoding RNAs of approximately 22-24 nucleotides which are capable of interacting with the 3' untranslated region of coding RNAs (mRNAs), leading to mRNA degradation and/or protein translation blockage. In recent years, differential microRNA expression in distinct cardiac development and disease contexts has been widely reported, yet the role of individual microRNAs in these settings remains largely unknown. We provide herein evidence of the role of miR-27 and miR-125 regulating distinct muscle-enriched transcription factors...
2015: BioMed Research International
Long Wang, Zhi-Ping Tang, Wei Zhao, Bing-Hai Cong, Jian-Qiang Lu, Xiao-Lu Tang, Xiao-Han Li, Xiao-Yan Zhu, Xin Ni
Hydrogen sulfide, generated in the myocardium predominantly via cystathionine-γ-lyase (CSE), is cardioprotective. Our previous study has shown that estrogens enhance CSE expression in myocardium of female rats. The present study aims to explore the mechanisms by which estrogens regulate CSE expression, in particular to clarify the role of estrogen receptor subtypes and the transcriptional factor responsible for the estrogenic effects. We found that either the CSE inhibitor or the CSE small interfering RNA attenuated the protective effect of 17β-estradiol (E2) against H2O2- and hypoxia/reoxygenation-induced injury in primary cultured neonatal cardiomyocytes...
June 2015: Endocrinology
Subbiah Ramasamy, Ganesan Velmurugan, K Shanmugha Rajan, Tharmarajan Ramprasath, Krishnan Kalpana
Physiological cardiac hypertrophy is an adaptive mechanism, induced during chronic exercise. As it is reversible and not associated with cardiomyocyte death, it is considered as a natural tactic to prevent cardiac dysfunction and failure. Though, different studies revealed the importance of microRNAs (miRNAs) in pathological hypertrophy, their role during physiological hypertrophy is largely unexplored. Hence, this study is aimed at revealing the global expression profile of miRNAs during physiological cardiac hypertrophy...
2015: PloS One
Marc N Hirt, Tessa Werner, Daniela Indenbirken, Malik Alawi, Paul Demin, Ann-Cathrin Kunze, Justus Stenzig, Jutta Starbatty, Arne Hansen, Jan Fiedler, Thomas Thum, Thomas Eschenhagen
Pathological cardiac hypertrophy and fibrosis are modulated by a set of microRNAs, most of which have been detected in biologically complex animal models of hypertrophy by arrays with moderate sensitivity and disregard of passenger strand (previously "star") microRNAs. Here, we aimed at precisely analyzing the microRNA signature of cardiac hypertrophy and fibrosis by RNA sequencing in a standardized in vitro hypertrophy model based on engineered heart tissue (EHT). Spontaneously beating, force-generating fibrin EHTs from neonatal rat heart cells were subjected to afterload enhancement for 7days (AE-EHT), and EHTs without intervention served as controls...
April 2015: Journal of Molecular and Cellular Cardiology
Lu Wang, Yurong Cui, Ming Tang, Xinwu Hu, Hongyan Luo, Jürgen Hescheler, Jiaoya Xi
AIMS: The embryonic stem cell-derived cardiomyocytes (ES-CM) is one of the promising cell sources for repopulation of damaged myocardium. However, ES-CMs present immature structure, which impairs their integration with host tissue and functional regeneration. This study used murine ES-CMs as an in vitro model of cardiomyogenesis to elucidate the effect of puerarin, the main compound found in the traditional Chinese medicine the herb Radix puerariae, on t-tubule development of murine ES-CMs...
2014: Cellular Physiology and Biochemistry
Yuliang Feng, Wei Huang, Mashhood Wani, Xiyong Yu, Muhammad Ashraf
Mesenchymal stem cells (MSCs) have potential application for the treatment of ischemic heart diseases. Besides differentiation properties, MSCs protect ischemic cardiomyocytes by secretion of paracrine factors. In this study, we found exosomes enriched with miR-22 were secreted by MSCs following ischemic preconditioning (Exo(IPC)) and mobilized to cardiomyocytes where they reduced their apoptosis due to ischemia. Interestingly, by time-lapse imaging, we for the first time captured the dynamic shedding of miR-22 loaded exosomes from cytosol to extracellular space...
2014: PloS One
Yingfeng Tu, Lin Wan, Dongliang Zhao, Lihong Bu, Dandan Dong, Zheyu Yin, Zhen Cheng, Baozhong Shen
PURPOSE: Growing evidence suggests that microRNAs (miRNAs) play key roles in cardiac hypertrophy. To measure the expression of endogenous miRNAs is very conducive to understanding the importance of miRNAs in cardiac hypertrophy. However, current methods to monitor endogenous miRNA levels, such as Northern blotting, quantitative real-time polymerase chain reaction (qRT-PCR), and microarrays cannot provide real-time information on miRNA biogenesis in vivo. METHODS: We constructed a miRNA reporter imaging system to monitor miR-22 expression in isoproterenol-induced cardiac hypertrophy repetitively and noninvasively...
May 2014: European Journal of Nuclear Medicine and Molecular Imaging
Jian Yang, Lihua Chen, Jun Yang, Jiawang Ding, Song Li, Hui Wu, Jing Zhang, Zhixing Fan, Wusong Dong, Xinxin Li
MicroRNAs are extensively involved in the pathogenesis of major cardiovascular diseases by suppressing target gene expression. Recent studies have reported that microRNA-22 (miR-22) may be implicated in ischemia-reperfusion (I/R) induced myocardial injury. However, the specific function of miR-22 in myocardial I/R injury is far from clear nowadays. The present study was designed to determine the role of miR-22 in myocardial I/R injury and investigate the underlying cardio-protective mechanism. The rat myocardial I/R injury model was induced by occluding the left anterior descending coronary artery for 30 min followed by 12 h reperfusion...
January 2014: Molecular Biology Reports
Priyatansh Gurha, Tiannan Wang, Ashley H Larimore, Yassine Sassi, Cei Abreu-Goodger, Maricela O Ramirez, Anilkumar K Reddy, Stefan Engelhardt, George E Taffet, Xander H T Wehrens, Mark L Entman, Antony Rodriguez
Increasing evidence suggests that microRNAs are intimately involved in the pathophysiology of heart failure. MicroRNA-22 (miR-22) is a muscle-enriched miRNA required for optimum cardiac gene transcription and adaptation to hemodynamic stress by pressure overload in mice. Recent evidence also suggests that miR-22 induces hypertrophic growth and it is oftentimes upregulated in end stage heart failure. However the scope of mRNA targets and networks of miR-22 in the heart failure remained unclear. We analyzed transgenic mice with enhanced levels of miR-22 expression in adult cardiomyocytes to identify important pathophysiologic targets of miR-22...
2013: PloS One
Xiaoming Zhu, Hongjiang Wang, Fan Liu, Li Chen, Weijia Luo, Pixiong Su, Weiming Li, Liping Yu, Xinchun Yang, Jun Cai
Micro-RNAs regulate gene expression by directly binding to the target mRNAs. The goal of the study was to examine the expression profiling of miRNAs in human failing hearts and identify the key miRNAs that regulate molecular signalling networks and thus contribute to this pathological process. The levels of miRNAs and expressed genes were analysed in myocardial biopsy samples from patients with end-stage heart failure (n = 14) and those from normal heart samples (n = 8). Four networks were built including the Gene regulatory network, Signal-Network, miRNA-GO-Network and miRNA-Gene-Network...
September 2013: Journal of Cellular and Molecular Medicine
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