keyword
https://read.qxmd.com/read/36935174/mir-18a-5p-aggravates-homocysteine-induced-myocardial-injury-via-autophagy
#21
JOURNAL ARTICLE
Juan Yin, Longlong Hu, Xueling Han, Lu Chen, Lingling Yu, Yinhui Lu
OBJECTIVES: Hyperhomocysteinaemia (Hcy) is an independent risk factor for cardiovascular and cerebrovascular diseases. MicroRNA (miR)-18a-5p is closely related to cardiovascular diseases. This study aims to investigate the effects of miR-18a-5p on homocysteine (Hcy)-induced myocardial cells injury. METHODS: H9c2 cells were transfected with miR-18a-5p mimic/miR-18a-5p mimic negative control (NC) or combined with Hcy for intervention, and untreated cells were set as a control group...
January 28, 2023: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://read.qxmd.com/read/36934412/microrna-17-3p-protects-against-excessive-posthypoxic-autophagy-in-h9c2-cardiomyocytes-via-pten-akt-mtor-signaling-pathway
#22
JOURNAL ARTICLE
Yi He, Dengwen Zhang, Qingqing Zhang, Yin Cai, Chongfeng Huang, Zhengyuan Xia, Sheng Wang
The activity of phosphatase and tensin homolog (PTEN) can be inhibited by miR-17-3p, which results in attenuating myocardial ischemia/reperfusion injury (IRI), however, the mechanism behind this phenomenon is still elusive. Suppression of PTEN leads to augmented protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling strength and constrained autophagy activation, which might be the one mechanism for the ameliorated myocardial IRI. Thus, we tested the hypothesis that miR-17-3p attenuated hypoxia/reoxygenation (H/R)-mediated damage in cardiomyocytes by downregulating excessive autophagy via the PTEN-Akt-mTOR axis...
May 2023: Cell Biology International
https://read.qxmd.com/read/36933242/microrna-362-3p-implicated-in-cardioprotection-against-hypoxia-reoxygenation-induced-cardiomyocyte-apoptosis-by-repressing-tp53inp2-and-regulating-sdf-1-cxcr4-pathway
#23
JOURNAL ARTICLE
Jinzheng Shi, Shaoqing Qin, Rui Wang, Zhanshuai Zhang, Huixian Li, Cuijun Hao
OBJECTIVE: To investigate the role of miR-362-3p and its target in cardiomyocytes with hypoxia/reoxygenation (H/R) injury. RESULTS: We found that miR-362-3p was decreased in myocardial infarction (MI) samples, and promoted the proliferation and restrained the apoptosis of H/R-injured H9c2 cells. TP53INP2 was recognized as the target of miR-362-3p and negatively modulated by miR-362-3p. Furthermore, the promotive effect of miR-362-3p on the proliferation of H/R-injured H9c2 cells was weakened by pcDNA3...
March 20, 2023: Alternative Therapies in Health and Medicine
https://read.qxmd.com/read/36910081/microrna-186-5p-inhibits-h9c2-cells-apoptosis-induced-by-oxygen-glucose-deprivation-by-targeting-erk1-2
#24
JOURNAL ARTICLE
Wennan Nie, Jia Wu, Xiaoyang Yu, Zhuolin Li, Xiaomin Cai, Wenyan Wei, Cheng Wang, Junjun Wang
BACKGROUND: Serum miR-186-5p levels are increased in acute myocardial infarction (AMI) patients and might contribute to assessing the prognosis of AMI patients. In this study, we further investigated the underlying molecular mechanism of miR-186-5p that participated in the pathological processes of myocardial ischemia. METHODS: The AMI models of rats and oxygen-glucose deprivation (OGD) models of H9c2 cells were established. Bioinformatics databases, luciferase reporting, and western blotting assays were performed to identify the regulatory target of miR-186-5p...
February 28, 2023: Journal of Thoracic Disease
https://read.qxmd.com/read/36817156/effects-of-astaxanthin-on-microrna-expression-in-a-rat-cardiomyocyte-anoxia-reoxygenation-model
#25
JOURNAL ARTICLE
Xinxin Zhang, Min Xu, Shuilin Cai, Bei Chen, Hetong Lin, Zhiyu Liu
Introduction: The protective effects of astaxanthin against myocardial ischemia-reperfusion injuries are well documented, although the mechanisms are not defined. Methods: The anoxia-reoxygenation injury model was established after astaxanthin treated H9c2 cells for 24 h. Cell viability, lactate dehydrogenase, oxidative stress level and western blot were tested. Secondly, measured the effects of astaxanthin pretreatment on microRNA expression in a rat myocardial cell anoxia-reoxygenation injury model. Results: After anoxia-reoxygenation injury, in a dose dependent manner, astaxanthin increased cell viability, superoxide dismutase and glutathione peroxidase activity, decreased lactate dehydrogenase and malondialdehyde levels, downregulated protein expression of caspase-3, caspase-8, nuclear factor erythroid-2-related factor 2 and heme oxygenase-1, and upregulated the Bcl-2/Bax ratio...
2023: Frontiers in Pharmacology
https://read.qxmd.com/read/36778043/knockdown-of-lncrna-snhg16-attenuates-myocardial-ischemia%C3%A2-reoxygenation-injury-via-targeting-mir%C3%A2-183-foxo1-axis
#26
JOURNAL ARTICLE
Tao Geng, Zesheng Xu, Jingxian Xing, Yonggang Yuan, Juan Liu
Accumulating evidence shows that long non-coding RNAs (lncRNAs) are widely involved in cellular processes of myocardial ischemia/reperfusion (I/R). The present study investigated the functions of lncRNA SNHG16 in myocardial I/R and the mechanism mediated by SNHG16. The myocardial I/R rat and cell model and hypoxia/reoxygenation injury (H/R) models of H9C2 cardiomyocytes were established to detect the expression of SNHG16. Cell Counting Kit-8, flow cytometric and western blot assays were conducted to detect cell viability, apoptosis and protein expression...
March 2023: Experimental and Therapeutic Medicine
https://read.qxmd.com/read/36772911/microrna-494-regulates-high-glucose-induced-cardiomyocyte-apoptosis-and-autophagy-by-pi3k-akt-mtor-signalling-pathway
#27
JOURNAL ARTICLE
Shuwei Ning, Siqi Zhang, Zhikun Guo
AIMS: Diabetic cardiomyopathy (DCM) is one of the major cardiovascular complications of diabetes. However, the mechanism of DCM is not fully understood. Studies have confirmed that certain microRNAs (miRNAs/miRs) are key regulators of DCM. The aim of this study was to investigate the role and mechanism of microRNA (miR)-494 in cardiomyocyte apoptosis and autophagy induced by high glucose (HG). METHODS AND RESULTS: By establishing a rat DCM model and an HG-treated H9c2 cells injury model, cardiac function was detected by echocardiography, myocardial tissue was stained by immunohistochemistry, and Cell Counting Kit-8 assay and lactate dehydrogenase assay were used to detect the cardiomyocyte injury...
February 11, 2023: ESC Heart Failure
https://read.qxmd.com/read/36598302/silencing-of-long-noncoding-rna-sox2-ot-relieves-myocardial-ischemia-reperfusion-injury-through-up-regulating-microrna-146a-5p
#28
JOURNAL ARTICLE
Zhongxin Li, Guangdong Liu, Hua Huang
PURPOSE: Long noncoding RNAs (lncRNAs) are involved in the development of myocardial ischemia/reperfusion injury (MIRI). In this study, we aimed to explore the regulatory effect and mechanism of lncRNA SOX2-OT in MIRI. METHODS: The expression levels of SOX2-OT and miR-146a-5p in OGD/R-treated H9C2 cells and in myocardial tissues of MIRI rats were measured by qRT-PCR. Cell viability was detected by MTT assay. The levels of IL-1β, IL-6, TNF-α, MDA, and SOD were measured by ELISA...
January 4, 2023: Bratislavské Lekárske Listy
https://read.qxmd.com/read/36562344/microrna%C3%A2-194%C3%A2-5p-attenuates-hypoxia-reoxygenation%C3%A2-induced-apoptosis-in-h9c2-cardiomyocytes-by-inhibiting-the-over%C3%A2-activation-of-rac1-protein
#29
JOURNAL ARTICLE
Cuie Li, Yinghua Li, Yanping Li, Yudi Wang, Yirong Teng, Yinglu Hao
Ras‑related C3 botulinum toxin substrate 1 (RAC1), a member of the Rac family of guanosine triphosphate phosphohydrolases, has been suggested to be a regulator of myocardial injury during ischemia and reperfusion (I/R). Whether microRNAs (miRs) are involved in the regulation of the aforementioned process remains to be elucidated. In the present study, an in vitro model of H9C2 cardiomyocytes was used to establish the overexpression of RAC1 following hypoxia and reoxygenation (H/R). Overexpression of RAC1 in H/R‑cultured cardiomyocytes could lead to cellular accumulation of reactive oxygen species (ROS) and facilitate the induction of apoptosis of H9C2 cardiomyocytes during H/R...
February 2023: Molecular Medicine Reports
https://read.qxmd.com/read/36524095/qishen-yiqi-dropping-pills-improve-cardiomyocyte-hypertrophy-via-the-lncrna-tincr-mir-193b-3p-rora-axis
#30
JOURNAL ARTICLE
Lei Chen, Lihua Pan, Yuansheng Zhang, Yuewu Chen, Yangshen Su, Ying Luo, Zengfan Wu, Wanling Zheng, Shikang Cai, Xianxia Liu, Xiaoyan Wu
BACKGROUND: This study was designed to explore the therapeutic effect and mechanism of action of Qishen Yiqi dropping pills (QYDP) in chronic heart failure (CHF) via a long non-coding RNA (lncRNA)-microRNA (miRNA)-messenger RNA (mRNA) axis. Here, the mechanism of action of the lncRNA terminal differentiation-induced non-coding RNA (TINCR), miR-193b-3p, and RAR-related orphan receptor A (RORA) mRNA was analyzed in an angiotensin (Ang) II-induced H9C2 cardiomyocyte hypertrophy model treated with QYDP...
November 2022: Journal of Thoracic Disease
https://read.qxmd.com/read/36293268/upregulation-of-reg-iv-and-hgf-mrnas-by-intermittent-hypoxia-via-downregulation-of-microrna-499-in-cardiomyocytes
#31
JOURNAL ARTICLE
Shin Takasawa, Asako Itaya-Hironaka, Mai Makino, Akiyo Yamauchi, Sumiyo Sakuramoto-Tsuchida, Tomoko Uchiyama, Ryogo Shobatake, Yoshinori Takeda, Hiroyo Ota
Sleep apnea syndrome (SAS) is characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]), and is a risk factor for cardiovascular disease (CVD) and insulin resistance/Type 2 diabetes. However, the mechanisms linking IH stress and CVD remain elusive. We exposed rat H9c2 and mouse P19.CL6 cardiomyocytes to experimental IH or normoxia for 24 h to analyze the mRNA expression of several cardiomyokines. We found that the mRNA levels of regenerating gene IV ( Reg IV ) and hepatocyte growth factor ( Hgf ) in H9c2 and P19...
October 17, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36246958/upregulation-of-mir-335-5p-contributes-to-right-ventricular-remodeling-via-calumenin-in-pulmonary-arterial-hypertension
#32
JOURNAL ARTICLE
Hong Ma, Peng Ye, Ai-Kai Zhang, Wan-de Yu, Song Lin, Ya-Guo Zheng
Right ventricular (RV) failure determines the prognosis in pulmonary arterial hypertension (PAH), but the underlying mechanism is still unclear. Growing evidence has shown that microRNAs participate in RV remodeling. This study is undertaken to explore the role of miR-335-5p in regulating RV remodeling induced by PAH. Two PAH models were used in the study, including the monocrotaline rat model and hypoxia/su5416 mouse model. miRNA sequencing and RT-qPCR validation identified that miR-335-5p was elevated in the RV of PAH rats...
2022: BioMed Research International
https://read.qxmd.com/read/36224710/overexpression-of-mir-383-3p-protects-cardiomyocytes-against-hypoxia-reoxygenation-injury-via-regulating-pten-pi3k-akt-signal-pathway
#33
JOURNAL ARTICLE
Huan Zeng, Ying Li, Xinzong Liu, Xinxin Li, Tian Zhou, Shanshan Cao, Mingjuan Wang, Mingfei Ju
MicroRNAs are widely reported as biomarkers and therapeutic targets in cardiovascular diseases. This study is aimed to expound on the regulatory responsibility of miR-383-3p in H/R-induced injury of H9c2 cells. In this study, H9c2 cells were administrated with H/R. MiR-383-3p expression was measured using qRT-PCR. ELISA was used to determine lactate dehydrogenase (LDH), superoxide dismutase (SOD), and malondialdehyde (MDA) levels. Reactive oxygen species (ROS) were detected with 2,7-Dichlorodihydrofluorescein diacetate probe...
October 12, 2022: Journal of Biochemical and Molecular Toxicology
https://read.qxmd.com/read/36163622/-lncrna-hotair-inhibits-the-proliferation-of-h9c2-rat-cardiomyocytes-induced-by-lipopolysaccharide-and-promotes-inflammation-through-targeting-negative-regulation-of-mir-1-3p
#34
JOURNAL ARTICLE
Xing Liu, Jiahui Dong, Yue Xian, Zongfang Ren
Objective To investigate whether long non-coding RNA-HOX transcript antisense intergenic RNA (lncRNA-HOTAIR) regulates the inflammatory response of sepsis by targeting microRNA miR-1-3p. Methods 0.5 μg/mL lipopolysaccharide (LPS) was used to induce cardiomyocytes of H9c2 rats to establish a septic cardiomyocyte injury model. H9c2 cells were divided into control group, LPS group, and LPS combined with HOTAIR small interfering RNA negative control (si-NC) group, LPS combined with HOTAIR small interfering RNA (si-HOTAIR) group, LPS combined with miR-1-3p negative control (miR-NC) group, LPS combined with miR-1-3p group, LPS combined with si-HOTAIR and miR-1-3p inhibitor negative (anti-miR-NC) group, and LPS combined with siHOTAIR and anti-miR-1-3p group...
October 2022: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://read.qxmd.com/read/36161101/mir-495-3p-depletion-contributes-to-myocardial-ischemia-reperfusion-injury-in-cardiomyocytes-by-targeting-tnc
#35
JOURNAL ARTICLE
Wei Song, Naiyan Qiu
Background: Tenascin-C (TNC) has been found to abnormally express in myocardial ischemia/reperfusion injury (MI/RI), but its effect on cardiomyocytes apoptosis is unknown and is worthy of investigation. Methods: H9C2 cells were given hypoxia/reoxygenation (H/R) treatment to obtain the replica of MI/RI in vitro . The effect of H/R on viability, apoptosis and inflammation was studied by CCK-8 assay, flow cytometry, mitochondrial membrane potential (MMP) and Ca2+ measurements as well as enzyme linked immunosorbent assay...
December 2022: Regenerative Therapy
https://read.qxmd.com/read/36125044/thyroid-stimulating-hormone-regulates-cardiac-function-through-modulating-hcn2-via-targeting-microrna-1a
#36
JOURNAL ARTICLE
Shengjie Zhang, Ran Li, Yiruo Ma, Ying Yan, Mei Ma, Keqin Zhang, Yun Zhou, Ling Li, Lingling Pan, Hao Ying, Ying Xue
Previous studies have found microRNA-1 (miR-1) and hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) may be involved in the pathogenesis of thyroid hormone (TH) induced cardiac hypertrophy. However, little is known about the role of miR-1 and HCN2 in thyroid stimulation hormone (TSH)-induced cardiac dysfunction. In order to investigate the molecular mechanisms of TSH induced cardiac dysfunction and the role of miR-1/HCN2 in that process, we evaluated the expression of miR-1a/HCN2 in the ventricular myocardium of hypothyroid mice and in TSH-stimulated H9c2 cardiomyocytes...
October 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/36098178/lncrna-foxd3-as1-aggravates-myocardial-ischemia-reperfusion-injury-by-inactivating-the-redd1-akt-gsk3%C3%AE-nrf2-signaling-pathway-via-the-mir-128-txnip-axis
#37
JOURNAL ARTICLE
Baozeng Chen, Lingling Zheng, Teng Zhu, Kai Jiao
Long noncoding RNA forkhead box D3-antisense RNA 1 (FOXD3-AS1) is associated with cardiovascular diseases, but its roles in myocardial ischemia/reperfusion (I/R) injury and the related signaling pathway have not been fully reported. We aimed to investigate the roles and mechanism of action of FOXD3-AS1 in myocardial I/R injury. An in vivo myocardial I/R injury mouse model and an in vitro hypoxia/reoxygenation (H/R) cardiomyocyte model was established. Quantitative reverse transcription-polymerase chain reaction, western blotting, and immunofluorescent assays were performed to examine the expression levels of FOXD3-AS1, microRNA (miR)-128, thioredoxin-interacting protein/regulation of development and DNA damage response 1/protein kinase B/glycogen synthase kinase 3β/nuclear factor erythroid 2-related factor 2 (TXNIP/Redd1/AKT/GSK3β/Nrf2) pathway-related proteins and apoptosis-related proteins...
September 13, 2022: Journal of Biochemical and Molecular Toxicology
https://read.qxmd.com/read/35986525/proteomic-analysis-of-glucose-induced-cardiac-myoblasts-and-the-potential-role-of-mir-92b-5p-in-regulating-sarcomere-proteins-under-hyperglycemic-environment
#38
JOURNAL ARTICLE
Priyanka Mathur, Vibha Rani
BACKGROUND: Diabetes mellitus, a co mMon metabolic disorder that causes high blood glucose, is due to impaired insulin secretion. Prolonged high blood sugar is associated with heart disease. Many proteins involved in metabolic pathways and contractility of cardiac cells regulate cardiac hypertrophy, altering normal cardiac physiology and function. Moreover, microRNAs are essential regulators of these proteins. Thus, there is a need to study the protein and microRNA alterations in cardiomyocytes to better understand the mechanisms activated during cardiac stress...
August 19, 2022: Protein and Peptide Letters
https://read.qxmd.com/read/35978367/mir-19b-3p-regulated-by-bc002059-abhd10-axis-promotes-cell-apoptosis-in-myocardial-infarction
#39
JOURNAL ARTICLE
Bihong Liao, Shaohong Dong, Zhenglei Xu, Fei Gao, Suihao Zhang, Ruijuan Liang
BACKGROUND: Recently, microRNAs (miRNAs), have been extensively investigated in diseases. The upregulated expression of miR-19b-3p has been validated in patients with hypertrophic cardiomyopathy. Nonetheless, it regulatory mechanism in myocardial infarction (MI) is still unclear. PURPOSE: This research aimed to investigate the role and molecular regulation mechanism of miR-19b-3p in MI. METHODS: QRT-PCR and western blot assays measured RNA and protein expression...
August 18, 2022: Biology Direct
https://read.qxmd.com/read/35965805/microrna-194-inhibits-isoproterenol-induced-chronic-cardiac-hypertrophy-via-targeting-cna-nfatc2-signaling-in-h9c2-cells
#40
JOURNAL ARTICLE
Jian Wang, Yucheng An, Jun Lin, Gang Tang
Background: This study explored the effects of microRNA(miR)-194 on chronic cardiac hypertrophy (CH) induced by isoproterenol (ISO). The potential mechanism through regulation of the calcineurin A (CnA)/nuclear factor of activated T cells (NFAT) c2 pathway was investigated in the rat cardiomyoblast cell line H9c2. Methods: H9c2 cells were treated with ISO to induce cardiomyocyte hypertrophy to simulate CH in vitro . The cell surface area was assessed by phalloidin staining...
July 2022: Annals of Translational Medicine
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