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Calcium dependent protein kinase

J Key, A K Mueller, S Gispert, L Matschke, I Wittig, O Corti, C Münch, N Decher, G Auburger
Parkinson's disease (PD) is the second most frequent neurodegenerative disorder in the old population. Among its monogenic variants, a frequent cause is a mutation in the Parkin gene (Prkn). Deficient function of Parkin triggers ubiquitous mitochondrial dysfunction and inflammation in the brain, but it remains unclear how selective neural circuits become vulnerable and finally undergo atrophy. We attempted to go beyond previous work mostly done in peripheral tumor cells, which identified protein targets of Parkin activity, an ubiquitin E3 ligase...
February 11, 2019: Neurobiology of Disease
Tingting Jia, Jian Wang, Wei Chang, Xiaoxu Fan, Xin Sui, Fuqiang Song
To reveal the mechanism of salinity stress alleviation by arbuscular mycorrhizal fungi (AMF), we investigated the growth parameter, soluble sugar, soluble protein, and protein abundance pattern of E. angustifolia seedlings that were cultured under salinity stress (300 mmol/L NaCl) and inoculated by Rhizophagus irregularis (RI). Furthermore, a label-free quantitative proteomics approach was used to reveal the stress-responsive proteins in the leaves of E. angustifolia . The result indicates that the abundance of 75 proteins in the leaves was significantly influenced when E...
February 12, 2019: International Journal of Molecular Sciences
Melanie Wong, Alexandra B Samal, Mike Lee, Jiri Vlach, Nikolai Novikov, Anita Niedziela-Majka, Joy Y Feng, Dmitry O Koltun, Katherine M Brendza, Hyock Joo Kwon, Brian E Schultz, Roman Sakowicz, Jamil S Saad, Giuseppe A Papalia
Calcium/calmodulin-dependent protein kinase II (CaMKII) is a multifunctional serine/threonine protein kinase that transmits calcium signals in various cellular processes. CaMKII is activated by calcium-bound calmodulin (Ca2+ /CaM) through a direct binding mechanism involving a regulatory C-terminal α-helix in CaMKII. The Ca2+ /CaM binding triggers transphosphorylation of critical threonine residues proximal to the CaM-binding site leading to the autoactivated state of CaMKII. The demonstration of its critical roles in pathophysiological processes has elevated CaMKII to a key target in the management of numerous diseases...
February 9, 2019: Journal of Molecular Biology
Nicolò Tosetti, Nicolas Dos Santos Pacheco, Dominique Soldati-Favre, Damien Jacot
Toxoplasma gondii possesses a limited set of actin-regulatory proteins and relies on only three formins (FRMs) to nucleate and polymerize actin. We combined filamentous actin (F-actin) chromobodies with gene disruption to assign specific populations of actin filaments to individual formins. FRM2 localizes to the apical juxtanuclear region and participates in apicoplast inheritance. Restricted to the residual body, FRM3 maintains the intravacuolar cell-cell communication. Conoidal FRM1 initiates a flux of F-actin crucial for motility, invasion and egress...
February 12, 2019: ELife
Briana Flaherty, Tienhuei G Ho, Sven Schmidt, Friedrich W Herberg, David Peterson, Eileen Kennedy
To explore the possibility of constrained peptides to target Plasmodium-infected cells, we designed a J domain mimetic derived from Plasmodium falciparum calcium-dependent protein kinase 1 (PfCDPK1) as a strategy to disrupt J domain binding and inhibit PfCDPK1 activity. The J domain disruptor (JDD) peptide was conformationally constrained using a hydrocarbon staple and was found to selectively permeate segmented schizonts and colocalize with intracellular merozoites in late-stage parasites. In vitro analyses demonstrated that JDD could effectively inhibit the catalytic activity of recombinant PfCDPK1 in the low micromolar range...
February 12, 2019: ACS Infectious Diseases
Jacques Demongeot, Hana Hasgui, Michel Thellier
-This paper presents a new model of memory functions in plants, which improves the previous approaches done by René Thomas and colleagues. We have decomposed the plant memory processing into three main functions: learning, storing and recalling. We propose as main mechanism for these three functions the occurrence of a calcium wave consecutive to an environmental stimulus, followed by the activation of specific genes and proteins changing their phosphorylated state. Feedback is ensured by Calcium Dependent Protein Kinases and oxidative phosphorylation...
February 8, 2019: Journal of Theoretical Biology
Sin Young Choi, Hae Jin Kee, Simei Sun, Young Mi Seok, Yuhee Ryu, Gwi Ran Kim, Seung-Jung Kee, Marc Pflieger, Thomas Kurz, Matthias U Kassack, Myung Ho Jeong
Here, we report that LMK235, a class I and histone deacetylase (HDAC6)-preferential HDAC inhibitor, reduces hypertension via inhibition of vascular contraction and vessel hypertrophy. Angiotensin II-infusion mice and spontaneously hypertensive rats (SHRs) were used to test the anti-hypertensive effect of LMK235. Daily injection of LMK235 lowered angiotensin II-induced systolic blood pressure (BP). A reduction in systolic BP in SHRs was observed on the second day when SHRs were treated with 3 mg/kg LMK235 every 3 days...
February 7, 2019: Journal of Cellular and Molecular Medicine
Guo-Rong Zhang, Hua Zhao, Eui M Choi, Michael Svestka, Xiaodan Wang, Aarti Nagayach, Anshuman Singh, Robert G Cook, Alfred I Geller
Advanced cognitive tasks are encoded in distributed neocortical circuits that span multiple forebrain areas. Nonetheless, synaptic plasticity and neural network theories hypothesize that essential information for performing these tasks is encoded in specific ensembles within these circuits. Relatively simpler subcortical areas contain specific ensembles that encode learning, suggesting that neocortical circuits contain such ensembles. Previously, using localized gene transfer of a constitutively active protein kinase C (PKC), we established that a genetically-modified circuit in rat postrhinal cortex, part of the hippocampal formation, can encode some essential information for performing specific visual shape discriminations...
February 7, 2019: Hippocampus
Wen-Fan Gao, Ya-Yun Xu, Jin-Fang Ge, Fei-Hu Chen
Acid‑sensing ion channel 1a (ASIC1a), member of the degenerin/epithelial sodium channel protein superfamily, serves a critical role in various physiological and pathological processes. The aim of the present study was to examine the role of ASIC1a in the autophagy of rat articular chondrocytes. Autophagy was induced by acidic stimulation in rat articular chondrocytes and the extent of autophagy was evaluated via the expression levels of microtubule‑associated protein 1 light chain 3II, Beclin1 and uncoordinated‑51 like kinase1...
January 31, 2019: International Journal of Molecular Medicine
Kenichi Ishizawa, Qin Wang, Jinping Li, Osamu Yamazaki, Yoshifuru Tamura, Yoshihide Fujigaki, Shunya Uchida, Richard P Lifton, Shigeru Shibata
Calcineurin is a calcium/calmodulin-regulated phosphatase known for its role in activation of T cells following engagement of the T cell receptor. Calcineurin inhibitors (CNIs) are widely used as immunosuppressive agents; common adverse effects of CNIs are hypertension and hyperkalemia. While previous studies have implicated activation of the Na-Cl cotransporter (NCC) in the renal distal convoluted tubule (DCT) in this toxicity, the molecular mechanism of this effect is unknown. The renal effects of CNIs mimic the hypertension and hyperkalemia that result from germ-line mutations in with-no-lysine (WNK) kinases and the Kelch-like 3 (KLHL3)-CUL3 ubiquitin ligase complex...
February 4, 2019: Proceedings of the National Academy of Sciences of the United States of America
Pei Zhang, Yuan Fan, Xiaopeng Sun, Lu Chen, William Terzaghi, Etienne Bucher, Lin Li, Mingqiu Dai
Drought is a major abiotic stress that threatens global food security. Circular RNAs (circRNAs) are endogenous RNAs. How these molecules influence plant stress responses remains elusive. Here, a large scale circRNA profiling identified 2174 and 1354 high-confidence circRNAs in maize and Arabidopsis, respectively, and most were differentially expressed in response to drought. A substantial number of drought-associated circRNA hosting genes were involved in conserved or species-specific pathways in drought responses...
February 4, 2019: Plant Journal: for Cell and Molecular Biology
Lidia Borkiewicz, Lidia Polkowska-Kowalczyk, Jarosław Cieśla, Paweł Sowiński, Maciej Jończyk, Wojciech Rymaszewski, Katarzyna P Szymańska, Radosław Jaźwiec, Grażyna Muszyńska, Jadwiga Szczegielniak
In plants, CALCIUM-DEPENDENT PROTEIN KINASES (CDPKs/CPKs) are involved in calcium signaling in response to endogenous and environmental stimuli. Here, we report that ZmCPK11, one of maize CDPKs, participates in salt stress response and tolerance. Salt stress induced expression and upregulated the activity of ZmCPK11 in maize roots and leaves. Activation of ZmCPK11 upon salt stress was also observed in roots and leaves of transgenic Arabidopsis plants expressing ZmCPK11. The transgenic plants showed a long-root phenotype under control conditions and a short-root phenotype under NaCl, abscisic acid (ABA) or jasmonic acid (JA) treatment...
February 4, 2019: Physiologia Plantarum
Regina Gratz, Prabha Manishankar, Rumen Ivanov, Philipp Köster, Inga Mohr, Ksenia Trofimov, Leonie Steinhorst, Johannes Meiser, Hans-Jörg Mai, Maria Drerup, Sibylle Arendt, Michael Holtkamp, Uwe Karst, Jörg Kudla, Petra Bauer, Tzvetina Brumbarova
Nutrient acquisition is entangled with growth and stress in sessile organisms. The bHLH transcription factor FIT is a key regulator of Arabidopsis iron (Fe) acquisition and post-translationally activated upon low Fe. We identified CBL-INTERACTING PROTEIN KINASE CIPK11 as a FIT interactor. Cytosolic Ca2+ concentration and CIPK11 expression are induced by Fe deficiency. cipk11 mutant plants display compromised root Fe mobilization and seed Fe content. Fe uptake is dependent on CBL1/CBL9. CIPK11 phosphorylates FIT at Ser272, and mutation of this target site modulates FIT nuclear accumulation, homo-dimerization, interaction with bHLH039, and transcriptional activity and affects the plant's Fe-uptake ability...
January 25, 2019: Developmental Cell
Christopher N Johnson
Modification of voltage-gated Na+ channel (NaV ) function by intracellular calcium ([Ca2+ ]i ) has been a topic of much controversy. Early studies relied on measuring NaV function in the absence or presence of [Ca2+ ]i , and generated seemingly disparate results. Subsequent investigations revealed the mechanism(s) of [Ca2+ ]i driven NaV modulation are complex and involve multiple accessory proteins. The Ca2+ sensing protein Calmodulin (CaM) has a central role in tuning NaV function to [Ca2+ ]i , but the mechanism has been obscured by other proteins (such as Fibroblast Growth Factors (FGF) or CaM dependent Kinase II (CaMKII)) as they can also modify channel function, or exert an influence in a Ca2+ dependent manner...
February 1, 2019: Journal of Physiology
Takuma Mori, Enas A Kasem, Emi Suzuki-Kouyama, Xueshan Cao, Xue Li, Taiga Kurihara, Takeshi Uemura, Toru Yanagawa, Katsuhiko Tabuchi
This article was originally published under standard licence, but has now been made available under a [CC BY 4.0] license. The PDF and HTML versions of the paper have been modified accordingly.
January 31, 2019: Molecular Psychiatry
Pavel A Brodskiy, Qinfeng Wu, Dharsan K Soundarrajan, Francisco J Huizar, Jianxu Chen, Peixian Liang, Cody Narciso, Megan K Levis, Ninfamaria Arredondo-Walsh, Danny Z Chen, Jeremiah J Zartman
The robust specification of organ development depends on coordinated cell-cell communication. This process requires signal integration among multiple pathways, relying on second messengers such as calcium ions. Calcium signaling encodes a significant portion of the cellular state by regulating transcription factors, enzymes, and cytoskeletal proteins. However, the relationships between the inputs specifying cell and organ development, calcium signaling dynamics, and final organ morphology are poorly understood...
January 11, 2019: Biophysical Journal
Alicia Bort, Belén G Sánchez, Elena Spínola, Pedro A Mateos-Gómez, Nieves Rodríguez-Henche, Inés Díaz-Laviada
Capsaicin is a natural compound present in chili and red peppers and the responsible of their spicy flavor. It has recently provoked interest because of its antitumoral effects in many cell types although its action mechanism is not clearly understood. As metabolic dysregulation is one of the hallmarks of cancer cells and the key metabolic sensor in the AMP-activated kinase (AMPK), in this study we explored the ability of capsaicin to modulate AMPK activity. We found that capsaicin activated AMPK in HepG2 cells by increasing AMPK phosphorylation and its downstream target ACC...
2019: PloS One
Keertan Dheda, Laura Lenders, Shashikant Srivastava, Gesham Magombedze, Helen Wainwright, Prithvi Raj, Stephen J Bush, Gabriele Pollara, Rachelle Steyn, Malika Davids, Anil Pooran, Timothy Pennel, Anthony Linegar, Ruth McNerney, Loven Moodley, Jotam G Pasipanodya, Carolin T Turner, Mahdad Noursadeghi, Robin M Warren, Edward Wakeland, Tawanda Gumbo
RATIONALE: There is poor understanding about protective immunity and the pathogenesis of cavitation in tuberculosis patients. OBJECTIVES: To map pathophysiological pathways at anatomically distinct positions within the human tuberculosis cavity. METHODS: Biopsies were obtained from eight pre-determined locations within lung cavities of multidrug-resistant tuberculosis patients undergoing therapeutic surgical resection (n=14) and healthy lung tissue from non-tuberculosis controls (n=10)...
January 29, 2019: American Journal of Respiratory and Critical Care Medicine
Claudio Tabolacci, Angelo De Martino, Carlo Mischiati, Giordana Feriotto, Simone Beninati
Tissue transglutaminase (transglutaminase type 2; TG2) is the most ubiquitously expressed member of the transglutaminase family (EC that catalyzes specific post-translational modifications of proteins through a calcium-dependent acyl-transfer reaction (transamidation). In addition, this enzyme displays multiple additional enzymatic activities, such as guanine nucleotide binding and hydrolysis, protein kinase, disulfide isomerase activities, and is involved in cell adhesion. Transglutaminase 2 has been reported as one of key enzymes that is involved in all stages of carcinogenesis; the molecular mechanisms of action and physiopathological effects depend on its expression or activities, cellular localization, and specific cancer model...
January 25, 2019: Medical Sciences: Open Access Journal
Udayakumar Karunakaran, Suma Elumalai, Jun Sung Moon, Jae Han Jeon, Nam Doo Kim, Keun Gyu Park, Kyu Chang Won, Jaechan Leem, In Kyu Lee
BACKGROUND: Chronic hyperglycemia has deleterious effects on pancreatic β-cell function and turnover. Recent studies support the view that cyclin-dependent kinase 5 (CDK5) plays a role in β-cell failure under hyperglycemic conditions. However, little is known about how CDK5 impair β-cell function. Myricetin, a natural flavonoid, has therapeutic potential for the treatment of type 2 diabetes mellitus. In this study, we examined the effect of myricetin on high glucose (HG)-induced β-cell apoptosis and explored the relationship between myricetin and CDK5...
January 16, 2019: Diabetes & Metabolism Journal
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