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Stem Cell banking

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https://read.qxmd.com/read/30900265/a-comparison-of-haematopoietic-stem-cells-from-umbilical-cord-blood-and-peripheral-blood-for-platelet-production-in-a-microfluidic-device
#1
Katrijn R Six, Géraldine Sicot, Rosalie Devloo, Hendrik B Feys, Dominique Baruch, Veerle Compernolle
BACKGROUND AND OBJECTIVES: Several sources of haematopoietic stem cells have been used for static culture of megakaryocytes to produce platelets in vitro. This study compares and characterizes platelets produced in shear flow using precursor cells from either umbilical (UCB) or adult peripheral blood (PB). MATERIALS AND METHODS: The efficiency of platelet production of the cultured cells was studied after perfusion in custom-built von Willebrand factor-coated microfluidic flow chambers...
March 21, 2019: Vox Sanguinis
https://read.qxmd.com/read/30888429/mammalian-haploid-stem-cells-establishment-engineering-and-applications
#2
REVIEW
Wenteng He, Jiayu Chen, Shaorong Gao
Haploid embryonic stem cells (haESCs) contain only one set of genomes inherited from the sperm or egg and are termed AG- or PG-haESCs, respectively. Mammalian haESCs show genome-wide hypomethylation and dysregulated imprinting, whereas they can sustain genome integrity during derivation and long-term propagation. In addition, haESCs exhibit similar pluripotency to traditional diploid ESCs but are unique because they function as gametes and have been used to produce semi-cloned animals. More strikingly, unisexual reproduction has been achieved in mice by using haESCs...
March 19, 2019: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/30806326/what-will-become-of-the-taxpayer-investment-in-public-cord-blood-stem-cell-banking
#3
David T Harris, Scott Israel
Cord blood (CB) is a unique and readily available source of hematopoietic stem cells for transplantation. CB also contains other types of stem cells, including endothelial stem cells and mesenchymal stem cells, that may prove useful in non-traditional clinical uses. Genetic and molecular analyses have demonstrated that CB stem cells lie somewhere between mature stem cells like those found in bone marrow (BM), and fetal stem cells. After 25 years of clinical experience CB is now used in the same fashion as BM for all typical malignant and genetic diseases treated by bone marrow transplant...
February 22, 2019: Current Stem Cell Research & Therapy
https://read.qxmd.com/read/30741433/contextual-factors-influencing-donor-recruitment-and-cord-blood-collection-perspectives-of-frontline-staff-of-the-canadian-blood-services-cord-blood-bank
#4
Jennie Haw, Jessica Polzer, Dana V Devine
BACKGROUND: Umbilical cord blood (CB) is an important source of hematopoietic stem cells that are used to treat blood- and immune-system disorders. Public CB banks aim to build inventories with high-quality CB units to meet healthcare needs. While research has noted the influence of broader contextual factors on donor recruitment and CB collection processes, to date, no published study has identified the specific contextual factors and challenges to donor recruitment and CB collection...
February 11, 2019: Transfusion
https://read.qxmd.com/read/30734294/evaluation-of-dmso-dextrose-as-a-suitable-alternative-for-dmso-dextran-in-cord-blood-cryopreservation
#5
Diego Raffo, Leticia Perez Tito, María Elisa Pes, Diego Fernandez Sasso
BACKGROUND AND OBJECTIVES: Umbilical cord blood is considered an alternative source of hematopoietic stem cells. Standard banking procedures use 50/55% DMSO in dextran 40 for cryopreservation and dextran-based solutions for thawing, however, due to the potential risk of crystallization of dextran, dextran 40 approved for clinical use has become limited or unavailable. This affects cryopreservation and thawing procedures. Carbohydrates, in particular sucrose, trehalose and glucose, have been shown to be effective in reducing cell damage during dehydration and have cryoprotective potential...
February 7, 2019: Vox Sanguinis
https://read.qxmd.com/read/30713041/reprogramming-captures-the-genetic-and-tumorigenic-properties-of-neurofibromatosis-type-1-plexiform-neurofibromas
#6
Meritxell Carrió, Helena Mazuelas, Yvonne Richaud-Patin, Bernat Gel, Ernest Terribas, Imma Rosas, Senda Jimenez-Delgado, Josep Biayna, Leen Vendredy, Ignacio Blanco, Elisabeth Castellanos, Conxi Lázaro, Ángel Raya, Eduard Serra
Neurofibromatosis type 1 (NF1) is a tumor predisposition genetic disease caused by mutations in the NF1 tumor suppressor gene. Plexiform neurofibromas (PNFs) are benign Schwann cell (SC) tumors of the peripheral nerve sheath that develop through NF1 inactivation and can progress toward a malignant soft tissue sarcoma. There is a lack of non-perishable model systems to investigate PNF development. We reprogrammed PNF-derived NF1(-/-) cells, descendants from the tumor originating cell. These NF1(-/-)-induced pluripotent stem cells (iPSCs) captured the genomic status of PNFs and were able to differentiate toward neural crest stem cells and further to SCs...
January 17, 2019: Stem Cell Reports
https://read.qxmd.com/read/30669334/the-future-state-of-newborn-stem-cell-banking
#7
Katherine S Brown, Mahendra S Rao, Heather L Brown
Newborn stem cell banking began with the establishment of cord blood banks more than 25 years ago. Over the course of nearly three decades, there has been considerable evolution in the clinical application of stem cells isolated from newborn tissues. The industry now finds itself at an inflection point as personalized medicine and regenerative medicine continue to advance. In this review, we summarize our perspective on newborn stem cell banking in the context of the future potential that stem cells from perinatal tissues are likely to play in nascent applications...
January 18, 2019: Journal of Clinical Medicine
https://read.qxmd.com/read/30665420/whole-blood-rna-sequencing-reveals-a-unique-transcriptomic-profile-in-patients-with-ards-following-hematopoietic-stem-cell-transplantation
#8
Joshua A Englert, Michael H Cho, Andrew E Lamb, Maya Shumyatcher, Diana Barragan-Bradford, Maria C Basil, Angelica Higuera, Colleen Isabelle, Mayra Pinilla Vera, Paul B Dieffenbach, Laura E Fredenburgh, Joyce B Kang, Ami S Bhatt, Joseph H Antin, Vincent T Ho, Robert J Soiffer, Judie A Howrylak, Blanca E Himes, Rebecca M Baron
BACKGROUND: The acute respiratory distress syndrome (ARDS) is characterized by the acute onset of hypoxemia and bilateral lung infiltrates in response to an inciting event, and is associated with high morbidity and mortality. Patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) are at increased risk for ARDS. We hypothesized that HSCT patients with ARDS would have a unique transcriptomic profile identifiable in peripheral blood compared to those that did not undergo HSCT...
January 21, 2019: Respiratory Research
https://read.qxmd.com/read/30646564/adoptive-t-cell-therapy-strategies-for-viral-infections-in-patients-receiving-haematopoietic-stem-cell-transplantation
#9
REVIEW
Giorgio Ottaviano, Robert Chiesa, Tobias Feuchtinger, Mark A Vickers, Anne Dickinson, Andrew R Gennery, Paul Veys, Stephen Todryk
Adverse outcomes following virus-associated disease in patients receiving allogeneic haematopoietic stem cell transplantation (HSCT) have encouraged strategies to control viral reactivation in immunosuppressed patients. However, despite timely treatment with antiviral medication, some viral infections remain refractory to treatment, which hampers outcomes after HSCT, and are responsible for a high proportion of transplant-related morbidity and mortality. Adoptive transfer of donor-derived lymphocytes aims to improve cellular immunity and to prevent or treat viral diseases after HSCT...
January 14, 2019: Cells
https://read.qxmd.com/read/30635361/a-rebuttal-to-akabayashi-and-colleagues-criticisms-of-the-ipsc-stock-project
#10
Misao Fujita, Keiichi Tabuchi
In the October edition of the Journal of Medical Ethics , Akabayashi and colleagues state that 'to establish a heterogeneous [induced pluripotent stem cell] iPSC bank covering roughly 80% of Japan's population…the Japanese government decided to invest JPY110 billion (US$ 1.1 billion) over 10 years in regenerative medicine research; a quarter of this was to be allocated to the iPSC stock project'. While they claim this amount of money to be an unfair distribution of state resources, we believe their assessment is based on a misunderstanding of the facts...
January 11, 2019: Journal of Medical Ethics
https://read.qxmd.com/read/30626756/organoid-single-cell-profiling-identifies-a-transcriptional-signature-of-glomerular-disease
#11
Jennifer L Harder, Rajasree Menon, Edgar A Otto, Jian Zhou, Sean Eddy, Noel L Wys, Christopher O'Connor, Jinghui Luo, Viji Nair, Cristina Cebrian, Jason R Spence, Markus Bitzer, Olga G Troyanskaya, Jeffrey B Hodgin, Roger C Wiggins, Benjamin S Freedman, Matthias Kretzler
Podocyte injury is central to many forms of kidney disease, but transcriptional signatures reflecting podocyte injury and compensation mechanisms are challenging to analyze in vivo. Human kidney organoids derived from pluripotent stem cells (PSCs), a potentially new model for disease and regeneration, present an opportunity to explore the transcriptional plasticity of podocytes. Here, transcriptional profiling of more than 12,000 single cells from human PSC-derived kidney organoid cultures was used to identify robust and reproducible cell lineage gene expression signatures shared with developing human kidneys based on trajectory analysis...
January 10, 2019: JCI Insight
https://read.qxmd.com/read/30581131/virus-specific-t-cells-current-and-future-use-in-primary-immunodeficiency-disorders
#12
REVIEW
Katherine M Harris, Blachy J Davila, Catherine M Bollard, Michael D Keller
Viral infections are common and can be potentially fatal in patients with primary immunodeficiency disorders (PIDDs). Because viral susceptibility stems from poor to absent T-cell function in most patients with moderate to severe forms of PIDD, adoptive immunotherapy with virus-specific T cells (VSTs) has been used to combat viral infections in the setting of hematopoietic stem cell transplantation in multiple clinical trials. Most trials to date have targeted cytomegalovirus, EBV, and adenovirus either alone or in combination, although newer trials have expanded the number of targeted pathogens...
December 20, 2018: Journal of Allergy and Clinical Immunology in Practice
https://read.qxmd.com/read/30567439/dental-stem-cells-current-research-and-future-applications
#13
V Campanella
The literature on "dental stem cells" from 2002 to 2009 is composed of 936 works, mainly basic research articles, 159 reviews, 1 clinical study and only 6 case reports concerning endodontics and periodontology. After the year 2009 (1) the interest on this research topic has increased leading to 5177 new articles, including 757 reviews on different stem cell types, biomaterials and the possibility of "banking". The 46 clinical trials published in the last 9 years are clinical applications in periodontics and endodontics...
December 2018: European Journal of Paediatric Dentistry: Official Journal of European Academy of Paediatric Dentistry
https://read.qxmd.com/read/30539429/sustainability-in-biobanking
#14
Maram Abdaljaleel, Elyse J Singer, William H Yong
Biobanks are storage places for biospecimens that can be used for current and future scientific research. Biospecimens are exceptional sources of biological data that can be potentially translated from molecular and genetic information to clinically relevant treatment modalities. Examples of such biospecimens include, but are not limited to, blood, skin, hair, saliva, stem cells, DNA, and RNA. The volume of biospecimens worldwide continues to grow at an extraordinary rate posing a challenge for biobanks to manage this growth...
2019: Methods in Molecular Biology
https://read.qxmd.com/read/30512203/edg5-plays-an-important-role-in-induction-and-maintenance-of-pluripotency
#15
Irina Neganova, Lewis Cotts, Peter Banks, Katja Gassner, Lyle Armstrong, Graham Ladds, Majlinda Lako
Direct reprogramming of human somatic cells toward induced pluripotent stem cells holds great promise for regenerative medicine and basic biology. We used a high-throughput siRNA screening assay in the initiation phase of reprogramming for 784 genes belonging to kinase and phosphatase families and identified 68 repressors and 22 effectors. Six new candidates belonging to the family of the G protein-coupled receptors (GPCRs) were identified suggesting an important role for this key signalling pathway during somatic cell induced reprogramming...
December 4, 2018: Stem Cells
https://read.qxmd.com/read/30498625/treatment-of-multiple-system-atrophy-the-past-present-and-future
#16
REVIEW
Haiyan Yu, Xiaoling Yuan, Lifeng Liu, Tian Wang, Dianrong Gong
Multiple system atrophy is a sporadic progressive degenerative disease which is characterized by multiple central nervous systems involved. So far, there is no effective medicine to cure MSA. The main research direction of treatment includes immunization transplantation and cytotherapy. Human umbilical cord blood is the residue of blood in the placenta and umbilical cord after fetal delivery. It is the most abundant cell bank and its usage is not limited to treat hematological diseases. The researches about hUCB-MNC treatment on MSA are increasing gradually...
2018: American Journal of Clinical and Experimental Immunology
https://read.qxmd.com/read/30485606/toward-the-development-of-the-true-off-the-shelf-synthetic-t-cell-immunotherapy
#17
Shoichi Iriguchi, Shin Kaneko
Recent outstanding clinical results demonstrated by engineered T cells, including chimeric antigen receptors, have already facilitated further researches that broaden their applicability. One such direction is to explore new T cell sources for allogeneic "off-the-shelf" adoptive immunotherapy. Human pluripotent stem cells may serve as an alternative cell source for this purpose due to its unique features of infinite propagation ability and pluripotency. Here, we describe the current state of engineered T cell transfer with the focus on cell manufacturing processes and the potentials and challenges of induced-pluripotent stem cell-derived T cells as a starting material to construct off-the-shelf T cell bank...
November 28, 2018: Cancer Science
https://read.qxmd.com/read/30449915/prospective-applications-of-induced-pluripotent-stem-cells-in-military-medicine
#18
REVIEW
Bitan Saha, H Krishna Kumar, Manash P Borgohain, Rajkumar P Thummer
Soldiers involved in combat operations worldwide may be subjected to a wide array of tissue-specific injuries of varying degrees, thereby undergoing complicated medical treatments and prolonged rehabilitations. In many cases involving inadequate recovery, soldiers are further mentally traumatized as they can no longer serve their beloved country. In addition, many severe injuries can lead to soldiers being incapacitated for life and unable to perform even the most basic day-to-day activities. Present therapy for combat injuries is majorly aimed at alleviating pain and limiting further tissue damage from secondary infections...
October 2018: Medical Journal, Armed Forces India
https://read.qxmd.com/read/30417867/efficient-and-scalable-directed-differentiation-of-clinically-compatible-corneal-limbal-epithelial-stem-cells-from-human-pluripotent-stem-cells
#19
Heidi Hongisto, Meri Vattulainen, Tanja Ilmarinen, Alexandra Mikhailova, Heli Skottman
Corneal limbal epithelial stem cells (LESCs) are responsible for continuously renewing the corneal epithelium, and thus maintaining corneal homeostasis and visual clarity. Human pluripotent stem cell (hPSC)-derived LESCs provide a promising cell source for corneal cell replacement therapy. Undefined, xenogeneic culture and differentiation conditions cause variation in research results and impede the clinical translation of hPSC-derived therapeutics. This protocol provides a reproducible and efficient method for hPSC-LESC differentiation under xeno- and feeder cell-free conditions...
October 24, 2018: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/30389588/workshop-report-cryopreservation-of-aquatic-biomedical-models
#20
Mary Hagedorn, Zoltan Varga, Ronald B Walter, Terrence R Tiersch
The genetic resources of aquatic biomedical model organisms are the products of millions of years of evolution, decades of scientific development, and hundreds of millions of dollars of research funding investment. Genetic resources (e.g., specific alleles, transgenes, or combinations) of each model organism can be considered a form of scientific wealth that can be accumulated and exchanged, typically in the form of live animals or germplasm. Large-scale maintenance of live aquatic organisms that carry these genetic resources is inefficient, costly, and risky...
October 31, 2018: Cryobiology
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