Stanislav S Terekhov, Yuliana A Mokrushina, Anton S Nazarov, Alexander Zlobin, Arthur Zalevsky, Gleb Bourenkov, Andrey Golovin, Alexey Belogurov, Ilya A Osterman, Alexandra A Kulikova, Vladimir A Mitkevich, Hua Jane Lou, Benjamin E Turk, Matthias Wilmanns, Ivan V Smirnov, Sidney Altman, Alexander G Gabibov
Microbial communities are self-controlled by repertoires of lethal agents, the antibiotics. In their turn, these antibiotics are regulated by bioscavengers that are selected in the course of evolution. Kinase-mediated phosphorylation represents one of the general strategies for the emergence of antibiotic resistance. A new subfamily of AmiN-like kinases, isolated from the Siberian bear microbiome, inactivates antibiotic amicoumacin by phosphorylation. The nanomolar substrate affinity defines AmiN as a phosphotransferase with a unique catalytic efficiency proximal to the diffusion limit...
June 2020: Science Advances