keyword
https://read.qxmd.com/read/38260503/sax-7-l1cam-acts-with-the-adherens-junction-proteins-magi-1-hmr-1-cadherin-and-afd-1-afadin-to-promote-glial-mediated-dendrite-extension
#21
Elizabeth R Cebul, Arthur Marivin, Leland R Wexler, Paola N Perrat, Claire Y Bénard, Mikel Garcia-Marcos, Maxwell G Heiman
Adherens junctions (AJs) are a fundamental organizing structure for multicellular life. Although AJs are studied mainly in epithelia, their core function - stabilizing cell contacts by coupling adhesion molecules to the cytoskeleton - is important in diverse tissues. We find that two C. elegans sensory neurons, URX and BAG, require conserved AJ proteins for dendrite morphogenesis. We previously showed that URX and BAG dendrites attach to the embryonic nose via the adhesion molecule SAX-7/L1CAM, acting both in neurons and glia, and then extend by stretch during embryo elongation...
January 11, 2024: bioRxiv
https://read.qxmd.com/read/38237738/nsun2-mediated-m-5-c-methylation-impairs-endometrial-receptivity
#22
JOURNAL ARTICLE
Jiafeng Lu, Ming Zhang, Zhenxing Liu, Ling Guo, Peng Huang, Wenjuan Xia, Jincheng Li, Jinghuan Lv, Hoi-Hung Cheung, Chenyue Ding, Hong Li, Boxian Huang
Impaired endometrial decidualization is the primary cause of recurrent implantation failure (RIF). RNA methylation modification, especially NSUN family mediated m5 C, is crucial for various physiological events, such as maternal-to-zygotic transition, gametogenesis, embryonic development, organismal lifespan, and cell cycle. However, the regulatory mechanisms between NSUN family mediated m5 C modification and RIF remain unknown. We acquired NSUN2 expression data of 15 human endometrium samples at proliferative and secretory stages from reproductive cell atlas...
January 17, 2024: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://read.qxmd.com/read/38214697/hb-egf-loaded-nanovesicles-enhance-trophectodermal-spheroid-attachment-and-invasion
#23
JOURNAL ARTICLE
Qi Hui Poh, Alin Rai, Jonathon Cross, David W Greening
The ability of trophectodermal cells (outer layer of the embryo) to attach to the endometrial cells and subsequently invade the underlying matrix are critical stages of embryo implantation during successful pregnancy establishment. Extracellular vesicles (EVs) have been implicated in embryo-maternal crosstalk, capable of reprogramming endometrial cells towards a pro-implantation signature and phenotype. However, challenges associated with EV yield and direct loading of biomolecules limit their therapeutic potential...
January 12, 2024: Proteomics
https://read.qxmd.com/read/38190636/identification-of-mosquito-eggshell-proteins-from-aedes-aegypti-by-liquid-chromatography-with-tandem-mass-spectrometry-lc-ms-ms-proteomic-analysis
#24
JOURNAL ARTICLE
Jun Isoe, Krishna Parsawar, Cynthia L David, Michael A Riehle, Roger L Miesfeld
The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal-ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos...
December 13, 2023: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38185104/three-dimensional-imaging-analysis-of-the-developmental-process-of-posterior-meniscofemoral-ligaments-in-rat-embryos
#25
JOURNAL ARTICLE
Momoko Nagai-Tanima, Kanon Ishida, Aoi Ishikawa, Shigehito Yamada, Tetsuya Takakuwa, Tomoki Aoyama
INTRODUCTION: The posterior meniscofemoral ligament (pMFL) of knee joint is a ligament that runs posterior to the posterior cruciate ligament (PCL) and it is known that the height of the pMFL attachment site causes meniscus avulsion. Therefore, understanding the three-dimensional (3D) structure of the pMFL attachment site is essential to better understand the pathogenesis of meniscus disorders. However, the developmental process of pMFL has not been well investigated. The purpose of this study was to analyze pMFL development in rat knee joints using 3D reconstructed images produced from episcopic fluorescence image capture (EFIC) images and examine its relationship with other knee joint components...
January 5, 2024: Cells, Tissues, Organs
https://read.qxmd.com/read/38171198/lncrna-stat3-as-regulates-endometrial-receptivity-via-the-stat3-signaling-pathway
#26
JOURNAL ARTICLE
Yanni Jia, Wei Wang, Jiaqi Jiang, Xinyan Zhang, Haijing Li, Suhua Gong, Zuhui Li, Haokun Liu, Chunmei Shang, Aihua Wang, Yaping Jin, Pengfei Lin
Endometrial receptivity is critical for the successful establishment of pregnancy in ruminants. Interferon tau (IFNT) plays a key role in promoting embryo attachment by activating the Janus kinase/signal transducer and activator of transcription pathway, which induces the expression of a series of interferon-stimulated genes (ISGs). In our previous study, sequencing analysis of goat endometrial epithelial cells (gEECs) treated with 20 ng/mL IFNT revealed a differentially expressed long non-coding RNA located on the STAT3 antisense chain, which we designated STAT3-AS...
December 29, 2023: Theriogenology
https://read.qxmd.com/read/38139454/multiple-mechanisms-controlling-uterine-function-editorial-to-special-issue-molecular-mechanisms-of-uterine-receptivity-for-embryo-implantation
#27
EDITORIAL
Jan Tesarik
Uterine (endometrial) receptivity is the ability of the endometrium to successfully attach to the embryo, to promote its implantation, to nourish it and keep it alive [...].
December 18, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38124757/how-to-eliminate-pathogen-without-killing-oneself-immunometabolism-of-encapsulation-and-melanization-in-drosophila
#28
JOURNAL ARTICLE
Tomas Dolezal
Cellular encapsulation associated with melanization is a crucial component of the immune response in insects, particularly against larger pathogens. The infection of a Drosophila larva by parasitoid wasps, like Leptopilina boulardi , is the most extensively studied example. In this case, the encapsulation and melanization of the parasitoid embryo is linked to the activation of plasmatocytes that attach to the surface of the parasitoid. Additionally, the differentiation of lamellocytes that encapsulate the parasitoid, along with crystal cells, is accountable for the melanization process...
2023: Frontiers in Immunology
https://read.qxmd.com/read/38112579/generation-of-porcine-endometrial-organoids-and-their-use-as-a-model-for-enhancing-embryonic-attachment-and-elongation
#29
JOURNAL ARTICLE
Islam M Saadeldin, Ayeong Han, Seonggyu Bang, Heejae Kang, Heyyoung Kim, Mariam M Abady, Ji-Seon Jeong, Ha-Jeong Kwon, Sanghoon Lee, JongKi Cho
Endometrial organoids (EOs) are acceptable models for understanding maternal-embryonic crosstalk. This study was conducted to generate EOs and optimize their cryopreservation and provide co-culture modeling with embryos. The endometrial tissues were used for culturing the organoids inside domes of Matrigel®. To improve the long-term storage of EOs, 10 µM ROCK inhibitor (RI) was added to the cryopreservation medium. Day-7 parthenogenetically activated embryos were co-cultured with EOs or EOs outgrowths, and embryonic cell numbers and embryo attachment were monitored...
December 1, 2023: Reproduction
https://read.qxmd.com/read/38101401/the-emergence-of-human-gastrulation-upon-in%C3%A2-vitro-attachment
#30
JOURNAL ARTICLE
Riccardo De Santis, Eleni Rice, Gist Croft, Min Yang, Edwin A Rosado-Olivieri, Ali H Brivanlou
While studied extensively in model systems, human gastrulation remains obscure. The scarcity of fetal biological material as well as ethical considerations limit our understanding of this process. In vitro attachment of natural blastocysts shed light on aspects of the second week of human development in the absence of the morphological manifestation of gastrulation. Stem cell-derived blastocyst models, blastoids, provide the opportunity to reconstitute pre- to post-implantation development in vitro...
December 6, 2023: Stem Cell Reports
https://read.qxmd.com/read/38079518/profiles-of-interferon-stimulated-genes-in-multiple-tissues-and-circulating-pregnancy-associated-glycoproteins-and-their-association-with-pregnancy-loss-in-dairy-cows
#31
JOURNAL ARTICLE
Rafael R Domingues, Joao Paulo N Andrade, Thiago O Cunha, Guilherme Madureira, August S Hoppman, Natalia N Teixeira, Pedro L J Monteiro, Victor H Gomez-Leon, Joao Paulo N Martins, Milo C Wiltbank
Pregnancy loss (PL) in lactating dairy cows disrupts reproductive and productive efficiency. We evaluated the expression of interferon-stimulated genes (ISG) in blood leukocytes, vaginal and cervical epithelial cells, luteolysis-related genes, progesterone and pregnancy-associated glycoproteins (PAG) profiles in lactating dairy cows (n = 86) to gain insight about PL. Expression of ISG on d17, d19, and d21 were greater in cows that maintained the pregnancy (P33) compared to nonpregnant with no PL (NP)...
December 11, 2023: Biology of Reproduction
https://read.qxmd.com/read/38070214/metabolic-stressful-environment-drives-epigenetic-modifications-in-oviduct-epithelial-cells
#32
JOURNAL ARTICLE
Patricia Kubo Fontes, Erika Cristina Dos Santos, Heloise Cale da Rocha, Camila Bruna de Lima, Marcella Pecora Milazzotto
The oviduct provides a suitable microenvironment from the gametes' final maturation until initial embryo development. Dynamic functional changes are observed in the oviduct cells, mainly controlled by steroid hormones and well-orchestrated during the estrous cycle. However, based on the roles played by the oviduct, additional layers of complexity might be present in its regulatory process. There is a cellular process that includes metabolic adaptation that can guide molecular modifications. This process is known as metaboloepigenetics...
December 3, 2023: Theriogenology
https://read.qxmd.com/read/38064193/understanding-placentation-in-ruminants-a-review-focusing-on-cows-and-sheep
#33
JOURNAL ARTICLE
Gregory A Johnson, Fuller W Bazer, Heewon Seo, Robert C Burghardt, Guoyao Wu, Ky G Pohler, Joe W Cain
Mammals differ regarding their placentae, but in all species placental trophoblasts interact intimately with the uterine endometrium to mediate the transfer of nutrients from the mother to the embryo/fetus through the closely juxtaposed microcirculatory systems of the uterus and placenta. Placentation in ruminants is intermediate between the non-invasive type, as observed in the epitheliochorial placenta of pigs, and the invasive type, as observed in the haemochorial placentae of mice and humans. In ruminants, placental trophoblast cells invade uterine endometrial tissue, but invasion is believed to be limited to the endometrial luminal epithelium (LE)...
December 2023: Reproduction, Fertility, and Development
https://read.qxmd.com/read/38049704/the-lncrna-linc00339-encoded-peptide-promotes-trophoblast-adhesion-to-endometrial-cells-via-mapk-and-pi3k-akt-signaling-pathways
#34
JOURNAL ARTICLE
Bo Zhou, Guo Yu, Mingqi Zhao, Yan Li, Jing Li, Yungai Xiang, Lili Tong, Xiying Chu, Caiyi Wang, Yuxia Song
BACKGROUND: Endometrial receptivity (ER), a pivotal event for successful embryo implantation, refers to the capacity of endometrium to allow the adhesion of the trophectoderm of the blastocyst to endometrial cells. In this paper, we set to elucidate whether the peptides encoded by lncRNAs could influence trophoblast cells' adhesion to endometrial cells. METHODS: WGCNA construction and bioinformatics were used to find out the ER-related lncRNAs with coding potential...
December 5, 2023: Journal of Assisted Reproduction and Genetics
https://read.qxmd.com/read/38047349/crmp5-participates-in-oocyte-meiosis-by-regulating-spastin-to-correct-microtubule-kinetochore-misconnection
#35
JOURNAL ARTICLE
Zhen Jin, Zhi-Cai Zhang, Chen-Yu Xiao, Mei-Qi Li, Qian-Ru Li, Lei-Lei Gao
Our previous studies have suggested that spastin, which aggregates on spindle microtubules in oocytes, may promote the assembly of mouse oocyte spindles by cutting microtubules. This action may be related to CRMP5, as knocking down CRMP5 results in reduced spindle microtubule density and maturation defects in oocytes. In this study, we found that, after knocking down CRMP5 in oocytes, spastin distribution shifted from the spindle to the spindle poles and errors in microtubule-kinetochore attachment appeared in oocyte spindles...
December 4, 2023: Zygote: the Biology of Gametes and Early Embryos
https://read.qxmd.com/read/38020065/peroxisome-proliferator-activated-receptor-%C3%AE-regulates-lipid-metabolism-in-sheep-trophoblast-cells-through-mtor-pathway-mediated-autophagy
#36
JOURNAL ARTICLE
Kexing Hao, Jing Wang, Hengbin Yu, Lei Chen, Weibin Zeng, Zhengrong Wang, Guangdong Hu
Peroxisome proliferator-activated receptor gamma (PPAR γ ) is a key nuclear receptor transcription factor that is highly expressed in trophoblastic cells during embryonic attachment and is accompanied by rapid cell proliferation and increased lipid accumulation. We previously showed that the autophagy pathway is activated in cells after activation of PPAR γ , accompanied by increased lipid accumulation. In this study, we used PPAR γ agonist rosiglitazone and inhibitor GW9662, as well as autophagy activator rapamycin and inhibitor 3-methyladenine, to unravel the probable mechanism of PPAR γ engaged in lipid metabolism in sheep trophoblast cells (STCs)...
2023: PPAR Research
https://read.qxmd.com/read/37962238/steroid-receptor-coactivator-2-drives-epithelial-reprogramming-that-enables-murine-embryo-implantation
#37
JOURNAL ARTICLE
Vineet K Maurya, Maria M Szwarc, David M Lonard, Ramakrishna Kommagani, San Pin Wu, Bert W O'Malley, Francesco J DeMayo, John P Lydon
Although we have shown that steroid receptor coactivator-2 (SRC-2), a member of the p160/SRC family of transcriptional coregulators, is essential for decidualization of both human and murine endometrial stromal cells, SRC-2's role in the earlier stages of the implantation process have not been adequately addressed. Using a conditional SRC-2 knockout mouse (SRC-2d/d ) in timed natural pregnancy studies, we show that endometrial SRC-2 is required for embryo attachment and adherence to the luminal epithelium. Implantation failure is associated with the persistent expression of Mucin 1 and E-cadherin on the apical surface and basolateral adherens junctions of the SRC-2d/d luminal epithelium, respectively...
December 2023: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/37950395/evaluation-of-vascular-endothelial-growth-factor-gene-expression-in-recellularized-liver-tissue-by-mouse-embryo-fibroblast
#38
JOURNAL ARTICLE
Motahare Homayoon Vala, Hamed Bagheri, Sargazi Zinat, Negar Bakhtiary, Shahram Pourbeiranvand, Mojdeh Salehnia
BACKGROUND: The aim of the present study was to evaluate alterations in the vegf gene expression as an angiogenic factor in mouse embryo fibroblasts seeded on the decellularized liver fragments. METHODS: Liver tissue samples (n = 10) collected from adult male mice were randomly divided into decellularized and native control groups. Tissues were decellularized by treating with 1% Triton X-100 and 0.1% SDS for 24 hours and assessed by H&E staining and SEM. Then DNA content analysis and toxicity tests were performed...
June 22, 2023: Iranian Biomedical Journal
https://read.qxmd.com/read/37934727/endometrial-glucose-metabolism-during-early-pregnancy
#39
JOURNAL ARTICLE
Ziting Chen, Matthew Dean
Approximately 50% of human pregnancies humans fail, most before or during implantation. One factor contributing to pregnancy loss is abnormal glucose metabolism in the endometrium. Glucose contributes to preimplantation embryo development, uterine receptivity, and attachment of the embryo. Across multiple species, the epithelium stores glucose as the macromolecule glycogen at estrus. This reserve is mobilized during the preimplantation period. Glucose from circulation or glycogenolysis can be secreted into the uterine lumen for use by the embryo or metabolized via glycolysis, producing ATP for the cell...
November 1, 2023: Reproduction & fertility
https://read.qxmd.com/read/37932098/phenotypic-analysis-of-preimplantation-lethality-in-mice
#40
JOURNAL ARTICLE
Virginia E Papaioannou, Richard R Behringer
Preimplantation development covers a period of ∼4.5 d from fertilization to implantation in the uterus. If a homozygous mutant phenotype causes the death of embryos during this period, simple culture methods are available that support preimplantation development to allow a thorough morphological assessment. Embryos are recovered from the oviducts or uterus and examined for gross morphology, cell number, and progression through cleavage stages. Blastocysts can also be cultured over the implantation period and undergo a process analogous to implantation in vitro...
November 6, 2023: Cold Spring Harbor Protocols
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