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t cell IL-8

Bin Zheng, Di Lou, Jianzu Ding, Xunhui Zhuo, Haojie Ding, Qingming Kong, Shaohong Lu
Toxoplasma gondii causes infections in a wide range of intermediate hosts and remains a threatening disease worldwide because of the lack of effective drugs and vaccines. Dense granule protein 24 (GRA24) is a novel essential virulence factor that is transferred into the nucleus of host cells from the parasitophorous vacuole to regulate gene expression. In the present study, bioinformatic analysis showed that GRA24 had a high score for B-cell and T-cell epitopes compared with surface antigen 1 (SAG1), which has been studied as a promising vaccine candidate...
2019: Frontiers in Immunology
Wendy Liermann, Jana Frahm, Ingrid Halle, Susanne Bühler, Jeannette Kluess, Liane Hüther, Sven Dänicke
The present study investigated clinical and immunological modulations due to intramuscular injection of Escherichia coli LPS in 49-wk-old laying hens over 48 h post injection (p.i.). LPS induced characteristic sickness behavior but no significant body temperature alterations ( P > 0.05). During experimental period decreases in blood albumin, calcium, phosphorus and tryptophan concentrations, hyperglycemia, increased plasma nitrite concentrations, leucopenia, decreased thrombocyte counts, lymphopenia, heterophilia and an increased heterophilic granulocyte/lymphocyte (H/L) ratio were observed after LPS administration...
April 2019: Innate Immunity
Pablo Tebas, Kimberly A Kraynyak, Ami Patel, Joel N Maslow, Matthew P Morrow, Albert J Sylvester, Dawson Knoblock, Elisabeth Gillespie, Dinah Amante, Trina Racine, Trevor McMullan, Moonsup Jeong, Christine C Roberts, Young K Park, Jean Boyer, Kate E Broderick, Gary P Kobinger, Mark Bagarazzi, David B Weiner, Niranjan Y Sardesai, Scott M White
BACKGROUND: Non-live vaccine approaches that are simple to deliver and stable at room temperature or 2-8°C could be advantageous in controlling future Ebola virus (EBOV) outbreaks. Using an immunopotent DNA vaccine that generates protection from lethal EBOV challenge in small animals and NHPs, we performed a clinical study to evaluate both intramuscular (IM) and novel intradermal (ID) DNA delivery. METHODS: Two DNA vaccine candidates (INO-4201 and INO-4202) targeting the EBOV glycoprotein (GP) were evaluated for safety, tolerability and immunogenicity in a Phase 1 clinical trial...
March 19, 2019: Journal of Infectious Diseases
Patrick M Schlievert, Michael P Cahill, Bruce S Hostager, Amanda J Brosnahan, Aloysius J Klingelhutz, Francoise A Gourronc, Gail A Bishop, Donald Y M Leung
Mucosal and skin tissues form barriers to infection by most bacterial pathogens. Staphylococcus aureus causes diseases across these barriers in part dependent on the proinflammatory properties of superantigens. We showed, through use of a CRISPR-Cas9 CD40 knockout, that the superantigens toxic shock syndrome toxin 1 (TSST-1) and staphylococcal enterotoxins (SEs) B and C stimulated chemokine production from human vaginal epithelial cells (HVECs) through human CD40. This response was enhanced by addition of antibodies against CD40 through an unknown mechanism...
March 19, 2019: MBio
Monique Garcia, Alana Santos-Dias, André Luis Lacerda Bachi, Manoel Carneiro Oliveira-Junior, Adilson Santos Andrade-Souza, Sérgio César Ferreira, Jefferson Comin Jonco Aquino-Junior, Francine Maria Almeida, Nicole Cristine Rigonato-Oliveira, Ana Paula Ligeiro Oliveira, Luiz Eduardo Baggio Savio, Robson Coutinho-Silva, Tobias Müller, Marco Idzko, Timo Siepmann, Rodolfo Paula Vieira
Creatine is a substrate for adenosine triphosphate synthesis, and it is the most used dietary supplement among professional and recreative athletes and sportsmen. Creatine supplementation may increase allergic airway response, but the cellular and molecular mechanisms are unknown. We used murine model of ovalbumin (OVA)-induced chronic asthma asthma and showed that creatine (Cr) supplementation increased total proteins, ATP level, lymphocytes, macrophages and IL-5 levels in bronchoalveolar lavage fluid, as well as IL-5 in the supernatant of re-stimulated mediastinal lymph nodes...
March 19, 2019: European Journal of Immunology
Sarah Fourour, Corinne Marois-Créhan, Léa Martelet, Christelle Fablet, Isabelle Kempf, Marcelo Gottschalk, Mariela Segura
Mycoplasma hyorhinis and M. flocculare are commonly co-isolated with M. hyopneumoniae (primary agent of swine enzootic pneumonia) in gross pneumonia-like lesions, but their involvement in the disease process remains unknown. T cells play an immuno-pathological role during mycoplasmal infections. Dendritic cells (DCs) are major antigen-presenting cells involved in T cell activation and differentiation. In this study, we investigated cytokine (IL-6, IL-8, IL-10, IL-12, and TNF-α) production by porcine bone-marrow-derived DCs (BM-DCs) stimulated by M...
March 16, 2019: Pathogens
Yan Zhang, Yu Yang, Xianduo Li, Dongdong Chen, Guanbao Tang, Tongyi Men
Chronic T cell mediated rejection (TCMR), which is characterized by infiltration of the interstitium by T cells and macrophages, still remains a major barrier to the long-term survival of kidney transplantation. Our recent report indicated that thalidomide can attenuate graft arteriosclerosis in an aortic transplant model. In this study, we investigated the effect of thalidomide on chronic TCMR in a rat model of kidney transplantation. Fischer or Lewis kidney allografts were transplanted into Lewis recipient rats...
March 12, 2019: International Immunopharmacology
Kalthoum Tizaoui, Seon Hui Kim, Gwang Hun Jeong, Andreas Kronbichler, Kwang Seob Lee, Keum Hwa Lee, Jae Il Shin
The 1858T allele in the protein tyrosine phosphatase non-receptor type 22 (PTPN22) locus shows one of the strongest and most consistent genetic associations with autoimmune diseases. We synthesized all meta-analyses reporting a genetic association of the PTPN22 1858T C/T polymorphism with autoimmune diseases. This work examined their validity to discover false positive results under Bayesian methods. We conducted a PubMed search to identify relevant publications and extracted the respective results, published until 30 November 2018...
March 12, 2019: Journal of Clinical Medicine
Sandra Palma, Nancy Chile, Rogger P Carmen-Orozco, Grace Trompeter, Kayla Fishbeck, Virginia Cooper, Laura Rapoport, Edson G Bernal-Teran, Beth J Condori, Robert H Gilman, Manuela R Verastegui
Taenia solium is known to cause human cysticercosis while T. saginata does not. Comparative in vitro and in vivo studies on the oncosphere and the postoncospheral (PO) forms of T. solium and T. saginata may help to elucidate why cysticercosis can occur from one and not the other. The aim of this study was to use in vitro culture assays and in vivo models to study the differences in the development of the T. solium and T. saginata oncosphere. Furthermore, this study aimed to evaluate the expression of cytokines and metalloproteinases (MMPs) in human peripheral blood mononuclear cells (PBMCs), which were stimulated by these oncospheres and PO antigens...
March 14, 2019: PLoS Neglected Tropical Diseases
Yaw Bediako, Rhys Adams, Adam J Reid, John Joseph Valletta, Francis M Ndungu, Jan Sodenkamp, Jedidah Mwacharo, Joyce Mwongeli Ngoi, Domtila Kimani, Oscar Kai, Juliana Wambua, George Nyangweso, Etienne P de Villiers, Mandy Sanders, Magda Ewa Lotkowska, Jing-Wen Lin, Sarah Manni, John W G Addy, Mario Recker, Chris Newbold, Matthew Berriman, Philip Bejon, Kevin Marsh, Jean Langhorne
BACKGROUND: There are over 200 million reported cases of malaria each year, and most children living in endemic areas will experience multiple episodes of clinical disease before puberty. We set out to understand how frequent clinical malaria, which elicits a strong inflammatory response, affects the immune system and whether these modifications are observable in the absence of detectable parasitaemia. METHODS: We used a multi-dimensional approach comprising whole blood transcriptomic, cellular and plasma cytokine analyses on a cohort of children living with endemic malaria, but uninfected at sampling, who had been under active surveillance for malaria for 8 years...
March 13, 2019: BMC Medicine
Masaya Matsuda, Tetsuya Terada, Nau Tsujimoto, Yuki Morie, Tomoki Ishida, Hiromu Takahashi, Junpei Hamaguchi, Yuki Tabuchi, Kana Doi, Keiki Noro, Yusuke Kikuoka, Shuji Omura, Takuya Yoshida, Yusuke Ayani, Manabu Suzuki, Takahiro Ichihara, Yuko Inaka, Takaki Inui, Ryo Kawata, Takeshi Nabe
AIM: The aim of this study was to clarify whether there are more regulatory T (Treg) and regulatory B (Breg) cells, and higher levels of IL-10-related transcription factors in subcutaneous immunotherapy (SCIT)-treated pollinosis patients than in non-SCIT-treated patients. METHODS: Japanese cedar pollinosis patients undergoing SCIT had received treatment for at least 2.8 years. Peripheral blood mononuclear cells were used for flow cytometer analyses and mRNA measurement...
April 2019: Immunotherapy
Ammoren Dohm, Jing Su, Emory R McTyre, James M Taylor, Lance D Miller, W Jeffrey Petty, Fei Xing, Hui-Wen Lo, Linda J Metheny-Barlow, Stacey O'Neill, Christina Bellinger, Travis Dotson, Boris Pasche, Kounosuke Watabe, Michael D Chan, Jimmy Ruiz
PURPOSE/OBJECTIVES: We aimed to assess the predictive value of a lung cancer gene panel for the development of brain metastases. MATERIALS/METHODS: Between 2011 and 2015, 102 patients with lung cancer were prospectively enrolled in a clinical trial in which a diagnostic fine-needle aspirate was obtained. Gene expression was conducted on all samples that rendered a diagnosis of non-small cell lung cancer (NSCLC). Subsequent retrospective analysis of brain metastases-related outcomes was performed by reviewing patient electronic medical records...
March 10, 2019: International Journal of Biological Markers
Haruo Usuda, Shimpei Watanabe, Masatoshi Saito, Shinichi Sato, Gabrielle C Musk, Erin Fee, Sean Carter, Yusaku Kumagai, Tsukasa Takahashi, Shinichi Kawamura, Takushi Hanita, Shigeo Kure, Nobuo Yaegashi, John P Newnham, Matthew W Kemp
BACKGROUND: Ex-vivo uterine environment (EVE) therapy is an experimental life support platform designed to reduce the risk of morbidity and mortality for extremely preterm infants born at the border of viability (21-24 weeks gestation). To spare the functionally immature lung, this platform performs gas exchange via a membranous oxygenator connected to the umbilical vessels, and the fetus is submerged in a protective bath of artificial amniotic fluid. We and others have demonstrated the feasibility of extended survival with EVE therapy in late preterm fetuses; however, there is presently no evidence to show that the use of such a platform can support extremely preterm fetuses, the eventual translational target for therapy of this nature...
March 7, 2019: American Journal of Obstetrics and Gynecology
Vikram Mehraj, Rayoun Ramendra, Stéphane Isnard, Franck P Dupuy, Bertrand Lebouché, Cecilia Costiniuk, Réjean Thomas, Jason Szabo, Jean-Guy Baril, Benoit Trottier, Pierre Coté, Roger LeBlanc, Madéleine Durand, Carl Chartrand-Lefebvre, Ido Kema, Yonglong Zhang, Malcolm Finkelman, Cécile Tremblay, Jean-Pierre Routy
Background: CXCL13 is preferentially secreted by Follicular Helper T cells (TFH ) to attract B cells to germinal centers. Plasma levels of CXCL13 have been reported to be elevated during chronic HIV-infection, however there is limited data on such elevation during early phases of infection and on the effect of ART. Moreover, the contribution of CXCL13 to disease progression and systemic immune activation have been partially defined. Herein, we assessed the relationship between plasma levels of CXCL13 and systemic immune activation...
2019: Frontiers in Immunology
Gholamreza Razmi, Saeed Yaghfoori, Mehrdad Mohri, Alirez Haghparast, Shahin Tajeri
Malignant ovine theileriosis is caused by Theileria lestoquardi, which is highly pathogenic in sheep. Theileriosis involves different organs in ruminants. Little is known about the role of proinflammatory cytokines in the pathogenesis of T. lestoquardi infection. The aim of this study was to measure concentration changes of proinflammatory cytokines and immunoglobulin G (IgG) during an ovine experimental theileriosis and correlate it with clinical and haematological parameters. During an experimental study, seven healthy Baluchi sheep (four females and three males) about 6-8 months old were infected with T...
February 4, 2019: Onderstepoort Journal of Veterinary Research
Florent Colomb, Leila B Giron, Thomas A Premeaux, Brooks I Mitchell, Toshiro Niki, Emmanouil Papasavvas, Luis J Montaner, Lishomwa C Ndhlovu, Mohamed Abdel-Mohsen
Endogenous plasma levels of the immunomodulatory carbohydrate-binding protein galectin-9 (Gal-9) are elevated during HIV infection and remain elevated after antiretroviral therapy (ART) suppression. We recently reported that Gal-9 regulates HIV transcription and potently reactivates latent HIV. However, the signaling mechanisms underlying Gal-9-mediated viral transcription remain unclear. Given that galectins are known to modulate T cell receptor (TCR)-signaling, we hypothesized that Gal-9 modulates HIV transcriptional activity, at least in part, through inducing TCR signaling pathways...
2019: Frontiers in Immunology
Rita R Alloway, E Steve Woodle, Daniel Abramowicz, Dorry L Segev, Remi Castan, Jillian N Ilsley, Kari Jeschke, K Troy Somerville, Daniel C Brennan
This report describes the results of two international randomized trials (total of 508 kidney transplant recipients). The primary objective was to assess the noninferiority of rabbit anti-thymocyte globulin (rATG, Thymoglobulin® ) vs interleukin-2 receptor antagonists (IL2RAs) for the quadruple endpoint (treatment failure defined as biopsy-proven acute rejection, graft loss, death, or loss to follow-up) to serve as the pivotal data for US regulatory approval of rATG. The pooled analysis provided an incidence of treatment failure of 25...
March 5, 2019: American Journal of Transplantation
Youn-Kwan Jung, Young-Mo Kang, Seungwoo Han
The enhanced differentiation and activation of osteoclasts (OCs) in the inflammatory arthritis such as rheumatoid arthritis (RA) and gout causes not only local bone erosion, but also systemic osteoporosis, leading to functional disabilities and morbidity. The induction and amplification of NFATc1, a master regulator of OC differentiation, is mainly regulated by receptor activator of NF-κB (RANK) ligand-RANK and calcium signaling which are amplified in the inflammatory milieu, as well as by inflammatory cytokines such as TNFα, IL-1β and IL-6...
February 2019: Immune Network
Ai Ling Liu, Hong Ying Wang, Hong Lü, Jia Ming Qian
Objective To explore whether aging increases severity of colitis in mice and its mechanism.Methods Young (6-8 weeks)and aged (56 weeks) C57Bl/6 mice were divided into the control and experimental group (n=5,each). Dextran sodium sulfate(DSS) was used to induce acute colitis mouse model in the experimental group.The mRNA expressions of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in colon were measured by RT-PCR. Tight junctions (TJs) of intestinal epithelial cells was examined by transmission electron microscopy (TEM)...
February 28, 2019: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
Vivian H M Mouser, Irene T M Arkesteijn, Bart G M van Dijk, Karin Wuertz-Kozak, Keita Ito
Inflammatory cytokines play an important role in intervertebral disc degeneration. Although largely produced by immune cells, nucleus pulposus (NP) cells can also secrete them under various conditions, e.g. under free swelling. Thus, tissue hypotonicity may be an inflammatory trigger for NP cells. The aim of this study was to investigate whether decreased tonicity under restricted swelling conditions (as occurring in early disc degeneration) could initiate an inflammatory cascade that mediates further degeneration...
March 5, 2019: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
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