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solid state fermentation

Jean-Félix Dallery, Géraldine Le Goff, Emilie Adelin, Bogdan I Iorga, Sandrine Pigné, Richard J O'Connell, Jamal Ouazzani
Colletotrichum higginsianum is the causal agent of crucifer anthracnose disease, responsible for important economic losses in Brassica crops. A mutant lacking the CclA subunit of the COMPASS complex was expected to undergo chromatin decondensation and the activation of cryptic secondary metabolite biosynthetic gene clusters. Liquid-state fermentation of the Δ cclA mutant coupled with in situ solid-phase extraction led to the production of three families of compounds, namely, colletorin and colletochlorin derivatives with two new representatives, colletorin D (1) and colletorin D acid (2), the diterpenoid α-pyrone higginsianin family with two new analogues, higginsianin C (3) and 13- epi-higginsianin C (4), and sclerosporide (5) coupling a sclerosporin moiety with dimethoxy inositol...
February 18, 2019: Journal of Natural Products
Krzysztof Kuglarz, Marek Bury, Agnieszka Kasprzycka, Justyna Lalak-Kańczugowska
The aim of the study was to determine the biogas productivity from selected cultivars of sorghum (Sorghum bicolor Moench) and maize (Zea mays L.) depending on the dose of mineral fertilization with nitrogen. The topic is novelty in the northern Poland area due to the fact that this crop is not very widespread here. The silage samples were derived from two experiments: 1) two factors experiment with sorghum varieties (Arbatax, KWS Maja, Herkules) and two doses of mineral nitrogen fertilization (0 and 150 kg·ha-1 N) in split-plot design...
February 15, 2019: Environmental Technology
T Bera, L Vardanyan, K S Inglett, K R Reddy, G A O'Connor, J E Erickson, A C Wilkie
Concerns about the negative impacts of crop biomass removal on soil ecological functions have led to questioning the long-term sustainability of bioenergy production. To offset this potential negative impact, use of organic C rich by-products from the bioenergy industries have been proposed as a means to replenish soil C in degraded soils. However, the impact of these by-products application on soil carbon dynamics is not fully understood. We measured biogeochemical changes in soil organic C following a three-year field application of two by-products, biochar (BC) and fermentation-by product (FBP), of bioenergy industry processes in an elephant grass [Pennisetum purpureum (L...
February 25, 2019: Science of the Total Environment
Caroline Torres de Oliveira, Eliana Albornoz Alves, Izelmar Todero, Raquel C Kuhn, Débora de Oliveira, Marcio A Mazutti
In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme was stable at a wide range of temperature and at all pH values tested...
February 9, 2019: Bioprocess and Biosystems Engineering
Guangsen Fan, Chao Teng, Dai Xu, Zhilei Fu, Pengxiao Liu, Qiuhua Wu, Ran Yang, Xiuting Li
Ethyl acetate content has strong influence on the style and quality of Baijiu . Therefore, this study investigated the effect of Saccharomyces cerevisiae Y3401 on the production of ethyl acetate by Wickerhamomyces anomalus Y3604. Analysis of cell growth showed that Y3401 influences Y3604 by nutrient competition and inhibition by metabolites, while the effect of Y3604 on Y3401 was mainly competition for nutrients. Mixed fermentation with two yeasts was found to produce more ethyl acetate than a single fermentation...
2019: BioMed Research International
Archana Gautam, Amit Kumar, Amit Kumar Bharti, Dharm Dutt
Rice straw is valuable resource that has been used as substrate for cost effective production of xylanase under solid-state fermentation by a newly isolated white rot fungi, S. commune ARC-11. Out of eleven carbon sources tested, rice straw was found most effective for the induction of xylanase that produced 4288.3 IU/gds of xylanase by S. commune ARC-11. Maximum xylanase production (6721.9 IU/gds) was observed on 8th day of incubation at temperature (30 °C), initial pH (7.0) and initial moisture content (70...
December 2018: Journal, Genetic Engineering & Biotechnology
Mutamed Ayyash, Stuart K Johnson, Shao-Quan Liu, Nouf Mesmari, Shaikhah Dahmani, Ayesha S Al Dhaheri, Jaleel Kizhakkayil
This study is an in-vitro investigation of the health-promoting properties of fermented whole-grain lupin, quinoa and wheat, using 72 h solid-state fermentation by Lactobacillus reuteri K777 and Lb. plantarum K779. Antiproliferative activity against Caco-2 and MCF-7 cancer cell lines (tumour cell lines of intestinal and mammary origin, respectively) was investigated, as well as α-amylase and α-glucosidase inhibition, antihypertensive, antioxidant and proteolytic activities. ABTS antioxidant activities of fermented lupin (FL, 55% w/v), quinoa (FQ, 55% w/v) and wheat (FW, 55% w/v) ranged from ∼12...
March 1, 2019: Food Chemistry
J J Muso Cachumba, R Terán Hilares, L P Brumano, P R F Marcelino, F A F Antunes, J C Santos, S S da Silva
L-asparaginase is an important enzyme used in the pharmaceutical and food industry, which can be produced by different microorganisms using low cost feedstocks. In this work, sugarcane bagasse (SCB) was used as support for enzyme production in solid-state fermentation (SSF) by A. terreus. Initially, the influence of the variables carbon and nitrogen sources on the enzyme production was studied following an experimental design carried out in Erlenmeyer flasks. Statistical analysis indicated the use of 0.54% of starch, 0% of maltose, 0...
February 3, 2019: Preparative Biochemistry & Biotechnology
Wan Yi Tai, Joo Shun Tan, Vuanghao Lim, Chee Keong Lee
The high cost of cellulases remains the most significant barrier to the economical production of bio-ethanol from lignocellulosic biomass. The goal of this study was to optimize cellulases and xylanase production by a local indigenous fungus strain (Aspergillus niger DWA8) using agricultural waste (oil palm frond (OPF)) as substrate. The enzyme production profile before optimization indicated that the highest carboxymethyl cellulose (CMCase), filter paper (FPase), and xylanase activities of 1.06 U/g, 2.55 U/g, and 2...
January 30, 2019: Biotechnology Progress
Zhi Na Yin, Wen Jia Wu, Chong Zhen Sun, Hui Fan Liu, Wen Bo Chen, Qi Ping Zhan, Zhuo Gui Lei, Xuan Xin, Juan Juan Ma, Kun Yao, Tian Min, Meng Meng Zhang, Hui Wu
OBJECTIVE: A strain of Aspergillus niger (A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain (FA-WB) were evaluated. METHODS: Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA) assays, and erythrocyte hemolysis assays...
January 2019: Biomedical and Environmental Sciences: BES
Abdalla Ali Salim, Sanja Grbavčić, Nataš aŠekuljica, Maja Vukašinović-Sekulić, Jelena Jovanović, Sonja Jakovetić Tanasković, Nevena Luković, Zorica Knežević-Jugović
Protease, cellulase and α-amylase producing B. subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determing pH, stirring rate, solid/liquid ratio and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE)...
January 29, 2019: Biotechnology and Applied Biochemistry
Lei Qiu, Jiao-Jiao Li, Zhen Li, Juan-Juan Wang
Brewer's spent grain (BSG) is a promising substrate for the production of biocontrol fertilizer (BF). The effects of temperature, water content and fermentation time on the conidiation and germination rate of the entomopathogenic fungi Beauveria bassiana (BbQLU1) were modeled in a 3 × 3 × 3 factorially designed experiment. The optimum conditions for BF production (60% water content at 25 °C for 12 days) resulted in a conidiation of 0.85 × 108 spores/g and a germination rate of 98.68%. BF at a concentration of 1 × 10-2 g/ml prompted plant growth and exhibited high toxicity against Galleria mellonella with an LT50 of 3...
January 24, 2019: Scientific Reports
Marina G Pessôa, Kele A C Vespermann, Bruno N Paulino, Mayara C S Barcelos, Glaucia M Pastore, Gustavo Molina
Nowadays, food, cosmetic, environmental and pharmaceutical fields are searching for alternative processes to obtain their major products in a more sustainable way. This fact is related to the increasing demand from the consumer market for natural products to substitute synthetic additives. Industrial biotechnology appears as a promising area for this purpose; however, the success of its application is highly dependent of the availability of a suitable microorganism. To overcome this drawback, the isolation of microorganisms from diverse sources, including fermented food, adverse environments, contaminated samples or agro-industrial wastes is an important approach that can provide a more adaptable strain able to be used as biocatalyst and that exhibit resistance to industrial conditions and high yields/productivities in biotechnological production of natural compounds...
January 18, 2019: Biotechnology Advances
Selvaraj Chinnathambi, Gert-Jan Euverink
Here, we report the engineering of a solid-state micro pH sensor based on polyaniline-functionalized, electrochemically reduced graphene oxide (ERGO-PA). Electrochemically reduced graphene oxide acts as the conducting layer and polyaniline acts as a pH-sensitive layer. The pH-dependent conductivity of polyaniline occurs by doping of holes during protonation and by the dedoping of holes during deprotonation. We found that an ERGO-PA solid-state electrode was not functional as such in fermentation processes. The electrochemically active species that the bacteria produce during the fermentation process interfere with the electrode response...
January 7, 2019: Journal of Visualized Experiments: JoVE
Guangyuan Jin, Yang Zhu, Arjen Rinzema, René H Wijffels, Xiangyang Ge, Yan Xu
Water is crucial for microbial growth, heat transfer and substrate hydrolysis, and dynamically changes with time in solid-state fermentation. However, water dynamics in the solid substrate is difficult to define and measure. Here, nuclear magnetic resonance was used to monitor water dynamics during the pure culture of Aspergillus oryzae YH6 on wheat in a model system to mimic solid starter (Qu or Koji) preparation. During fermentation, overall water content gradually decreased from 0.84 to 0.36 g/g, and water activity decreased from 0...
January 9, 2019: Bioresource Technology
Somchai Rice, Madina Tursumbayeva, Matthew Clark, David Greenlee, Murlidhar Dharmadhikari, Anne Fennell, Jacek A Koziel
The Midwest wine industry has shown a marked increase in growers, hectares planted, wineries, and wine production. This growth coincides with the release of cold-hardy cultivars such as Brianna and Frontenac gris, in 2001 and 2003, respectively. These white grape varieties account for one-third of the total area grown in the state of Iowa. It is generally accepted that the wine aroma profile plays a crucial role in developing a local, sustainable brand. However, the identity of Brianna/Frontenac Gris-based wine aromas and their link to the grape berry chemistry at harvest is unknown...
January 16, 2019: Foods (Basel, Switzerland)
Guanhua Li, Ying Fu, Wenxian Dang, Ruiping Hu, Huiting Xue
Chemical composition and physical structure of solid substrate have significantly impacts on fermentation performance. The aqueous ammonia was used to pretreat rice straw. Furthermore, the feasibility of pretreatment to improve laccase production was also evaluated in terms of the enzymatic digestibility, chemical structure, physical structure, and laccase production. The results showed that aqueous ammonia pretreatment could modify chemical compositions, destroy rigid structure of the lignocellulosic substrate, increase enzymatic digestibility and change water state, which were beneficial to facilitate the fungus growth and nutrition utilization...
January 17, 2019: Bioprocess and Biosystems Engineering
Phongsakorn Kunhorm, Nipha Chaicharoenaudomrung, Parinya Noisa
Cordyceps spp. is the herbal medication initially used in China and has been reported as the unique resource of cordycepin. Cordycepin exhibits many health benefits, including anti-photoaging and anti-pigmentation; therefore, it potentially is a bioactive ingredient of cosmetic products. In order to enrich cordycepin content in Cordyceps, two artificial cultivation procedures, which are solid-state fermentation and liquid culture, were developed and optimized. The aim of this review is to illustrate cordycepin biosynthesis pathway in Cordyceps, and its bioactivity for cosmeceutical applications, as well as comparing the two different cultivation procedures...
January 15, 2019: Applied Microbiology and Biotechnology
Houthail Alahmad Aljammas, Hassan Al Fathi, Walid Alkhalaf
Investigations were conducted on the production of Rennin enzyme from the fungi Rhizomucor miehei 3420 NRRL using Solid-State fermentation. Wheat bran was used as a substrate. The influence of moisture content, incubation temperature, and the initial pH of fermentation medium were studied. The protein content, milk clotting activity (MCA), specific activity, proteolytic activity (PA), and (MCA/PA) ratio of the extracted enzyme were calculated after 4 days of incubation to evaluate the quality of the enzyme...
June 2018: Journal, Genetic Engineering & Biotechnology
Shruti Pathania, Nivedita Sharma, Shweta Handa
The brown rot fungus Rhizopus delemar F2 was shown to produce extracellular thermostable and multiple carbohydrase enzymes. The potential of Rhizopus delemar F2 in utilizing apple pomace under solid state fermentation (SSF) is the purpose of the study. Solid state fermentation (SSF) is a very effective technique opposed to submerged fermentation in various aspects. Enhanced production of multiple carbohydrases 18.20 U g-1 of cellulose, 158.30 U g-1 of xylanase, 61.50 U g-1 of pectinase and amylase 21...
June 2018: Journal, Genetic Engineering & Biotechnology
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