Komal Farooq, Zahid Anwar, Waseem Khalid, Shoaib Hasan, Fareed Afzal, Muddassar Zafar, Usman Ali, Othman Alghamdi, Ammar Al-Farga, Saleh M Al-Maaqar
The biotechnological process called solid-state fermentation (SSF) was applied for hyper production of protease by using a fungal strain called Aspergillus oryzae . From screening of 9 different local substrates (peanut shell, wheat bran, guava leaves, sugar cane bagasse, rice polish, wheat straw, corn straw, reed grass, and rice straw), peanut shells serve as the best substrates for protease production under optimized cultured conditions. The varying physiochemical parameters such as pH (2-9.5), temperature (30-52 °C), incubation time (1-10 days), inoculum size (1-8 mL), moisture level (20-125%), and substrate concentration (1-7 g) were optimized by response surface methodology (RSM)...
April 16, 2024: ACS Omega