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Keywords Excisional Wound Healing In Th...

Excisional Wound Healing In The Mouse

https://read.qxmd.com/read/38472732/exosomes-derived-from-mouse-breast-carcinoma-cells-facilitate-diabetic-wound-healing
#1
JOURNAL ARTICLE
Chao Zhang, Wenchi Xiao, Hao Wang, Linxiao Li, Yan Yang, Yongwei Hao, Zhihao Xu, Hongli Chen, Wenbin Nan
BACKGROUND: Exosomes derived from breast cancer have been reported to play a role in promoting cell proliferation, migration, and angiogenesis, which has the potential to accelerate the healing process of diabetic wounds. The aim of this investigation was to examine the function of exosomes originating from 4T1 mouse breast carcinoma cells (TEXs) in the process of diabetic wound healing. METHODS: The assessment of primary mouse skin fibroblasts cell proliferation and migration was conducted through the utilization of CCK-8 and wound healing assays, while the tube formation of HUVECs was evaluated by tube formation assay...
March 12, 2024: Tissue Engineering and Regenerative Medicine
https://read.qxmd.com/read/38340904/1-25-oh-2-d-3-improves-diabetic-wound-healing-by-modulating-inflammation-and-promoting-angiogenesis
#2
JOURNAL ARTICLE
Yiming Ma, Yiting Gong, Ying Wu, Qiaofan Zhao, Ruyu Fu, Xiaoming Zhang, Ye Li, Xueyuan Zhi
Vitamin D was found to regulate inflammatory response and angiogenesis, which were often impaired in diabetic wound healing. This study aimed to investigate the effects of 1,25-dihydroxyvitamin D3 [1,25(OH)2 D3 ] on diabetic wound healing both in vivo and in vitro. Diabetes was induced by high-fat diet combined with streptozotocin. After four weeks of establishing diabetic mouse model, full-thickness excisional wounds were created on their dorsal skin. Then 1,25(OH)2 D3 was administered via intraperitoneal injection for 14 consecutive days...
February 8, 2024: Journal of Steroid Biochemistry and Molecular Biology
https://read.qxmd.com/read/38313508/in-situ-formation-of-hydrogel-wound-dressing-based-on-carboxymethyl-chitin-tannic-acid-for-promoting-skin-wound-healing
#3
JOURNAL ARTICLE
Jinhui Lin, Siyaqi Li, Yunfei Ying, Weilin Zheng, Jingcheng Wu, Peiyuan Wang, Xiaolong Liu
Triggering the healing process of drug-resistant bacteria-infected wounds has attracted great attention due to global morbidity that may induce gangrene, amputation, and even death. Here, a chitin derivative, carboxymethyl chitosan (CMC), tannic acid (TA), and Cu2+ were used for hydrogel engineering. Using sodium bicarbonate as the neutralizer and reductant, hydrogen bonds between CMC and TA and in situ Cu(OH)2 generation via ion coordination force between Cu2+ and TA facilitated the synthesis of CMC/TA/Cu hydrogel...
January 30, 2024: ACS Omega
https://read.qxmd.com/read/38307430/exploration-of-the-anti-inflammatory-analgesic-and-wound-healing-activities-of-bletilla-striata-polysaccharide
#4
JOURNAL ARTICLE
Xiaomei He, Longyun Liu, Fangli Gu, Renshu Huang, Li Liu, Yuting Nian, Yingyu Zhang, Cheng Song
Bletilla Striata (BS) Polysaccharide (BSP) is one of the main components of the traditional Chinese medicinal plant Bletilla striata Rchb. F. BSP has been widely used in antimicrobial and hemostasis treatments in clinics. Despite its use in skin disease treatment and cosmetology, the effects of BSP on wound healing remain unclear. Here we investigated the anti-inflammatory, antioxidant, and analgesic effects of BSP and explored its impact on morphological changes and inflammatory mediators during wound healing...
January 31, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38257315/assessment-of-tilapia-skin-collagen-for-biomedical-research-applications-in-comparison-with-mammalian-collagen
#5
JOURNAL ARTICLE
Jyun-Yuan Huang, Tzyy-Yue Wong, Ting-Yuan Tu, Ming-Jer Tang, Hsi-Hui Lin, Yuan-Yu Hsueh
Collagen is an important material for biomedical research, but using mammalian tissue-derived collagen carries the risk of zoonotic disease transmission. Marine organisms, such as farmed tilapia, have emerged as a safe alternative source of collagen for biomedical research. However, the tilapia collagen products for biomedical research are rare, and their biological functions remain largely unexamined. In this study, we characterized a commercial tilapia skin collagen using SDS-PAGE and fibril formation assays and evaluated its effects on skin fibroblast adhesion, proliferation, and migration, comparing it with commercial collagen from rat tails, porcine skin, and bovine skin...
January 13, 2024: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/38236539/in-vivo-wound-healing-model-for-characterization-of-gene-electrotransfer-effects-in-mouse-skin
#6
JOURNAL ARTICLE
Špela Kos, Tanja Jesenko, Tanja Blagus
Wound healing is a complex biological response to injury characterized by a sequence of interdependent and overlapping physiological actions. To study wound healing and cutaneous regeneration processes, the complexity of wound healing requires the use of animal models. In this chapter, we describe the protocol to generate skin wounds in a mouse model. In the mouse splinted excisional wound model, two full-thickness wounds are firstly created on the mouse dorsum, which is followed by application of silicone splint around wounded area...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38155595/a-recombinant-signalling-selective-activated-protein-c-that-lacks-anticoagulant-activity-is-efficacious-and-safe-in-cutaneous-wound-preclinical-models
#7
JOURNAL ARTICLE
Ruilong Zhao, Meilang Xue, Haiyan Lin, Margaret Smith, Helena Liang, Hartmut Weiler, John H Griffin, Christopher J Jackson
Various preclinical and clinical studies have demonstrated the robust wound healing capacity of the natural anticoagulant activated protein C (APC). A bioengineered APC variant designated 3K3A-APC retains APC's cytoprotective cell signalling actions with <10% anticoagulant activity. This study was aimed to provide preclinical evidence that 3K3A-APC is efficacious and safe as a wound healing agent. 3K3A-APC, like wild-type APC, demonstrated positive effects on proliferation of human skin cells (keratinocytes, endothelial cells, and fibroblasts)...
December 29, 2023: Wound Repair and Regeneration
https://read.qxmd.com/read/38139127/impact-of-a-high-fat-diet-at-a-young-age-on-wound-healing-in-mice
#8
JOURNAL ARTICLE
Kevin Arnke, Pablo Pfister, Gregory Reid, Mauro Vasella, Tim Ruhl, Ann-Kathrin Seitz, Nicole Lindenblatt, Paolo Cinelli, Bong-Sung Kim
As the prevalence of juvenile-onset obesity rises globally, the multitude of related health consequences gain significant importance. In this context, obesity is associated with impaired cutaneous wound healing. In experimental settings, mice are the most frequently used model for investigating the effect of high-fat diet (HFD) chow on wound healing in wild-type or genetically manipulated animals, e.g., diabetic ob / ob and db / db mice. However, these studies have mainly been performed on adult animals. Thus, in the present study, we introduced a mouse model for a juvenile onset of obesity...
December 9, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38112377/rapid-semiconducting-supramolecular-mg-ii-metallohydrogel-exploring-its-potential-in-nonvolatile-resistive-switching-applications-and-antiseptic-wound-healing-properties
#9
JOURNAL ARTICLE
Subhendu Dhibar, Arpita Roy, Tuhin Sarkar, Priyanka Das, Kripasindhu Karmakar, Subham Bhattacharjee, Bijnaneswar Mondal, Priyajit Chatterjee, Keka Sarkar, Soumya Jyoti Ray, Bidyut Saha
An effective strategy was employed for the rapid development of a supramolecular metallohydrogel of Mg(II) ion (i.e., Mg@PEHA) using pentaethylenehexamine (PEHA) as a low-molecular-weight gelator in aqueous medium under ambient conditions. The mechanical stability of the synthesized Mg@PEHA metallohydrogel was characterized by using rheological analysis, which showed its robustness across different angular frequencies and oscillator stress levels. The metallohydrogel exhibited excellent thixotropic behavior, which signifies that Mg@PEHA has a self-healing nature...
December 19, 2023: Langmuir: the ACS Journal of Surfaces and Colloids
https://read.qxmd.com/read/38106058/commensal-skin-bacteria-exacerbate-inflammation-and-delay-skin-healing
#10
Veda D Khadka, Laura Markey, Magalie Boucher, Tami D Lieberman
The skin microbiome can both trigger beneficial immune stimulation and pose a potential infection threat. Previous studies have shown that colonization of mouse skin with the model human skin commensal Staphylococcus epidermidis is protective against subsequent excisional wound or pathogen challenge. However, less is known about concurrent skin damage and exposure to commensal microbes, despite growing interest in interventional probiotic therapy. Here, we address this open question by applying commensal skin bacteria at a high dose to abraded skin...
December 4, 2023: bioRxiv
https://read.qxmd.com/read/37952806/branched-fructo-oligosaccharides-from-polygonatum-cyrtonema-hua-as-crosslinking-agents-for-cellulose-a-novel-injectable-and-on-demand-dissolution-hydrogel-for-diabetic-wound
#11
JOURNAL ARTICLE
Hao Ma, Lili He, Yi Yang, Tiantian Wang, Jiayao Li, Zhifeng Zhang, Pei Luo
Oligosaccharide-containing macromolecular bio adhesives are emerging as highly promising eco-friendly materials to enhance the cytocompatibility of viscous hydrogels for wound healing applications. In our prior research, we extensively elucidated the properties of branch-structure fructo-oligosaccharides derived from Polygonatum Cyrtonema Hua (referred to as PCOS). However, the characteristics of hydrogels based on fructo-oligosaccharides remain to be fully explored. In present work, we developed an injectable, PCOS/carboxymethyl cellulose (CMC) hydrogel which is a dual ion-physical cross-linked hydrogel that can be considered as a potential diabetic wound dressing...
November 10, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/37541071/lychnophora-ericoides-mart-brazilian-arnica-ethanol-extract-accelerates-the-skin-wound-healing-process-evidence-for-its-mechanism-of-action
#12
JOURNAL ARTICLE
Barbhara Mota Marinho, Victor Hugo Dantas Guimarães, Daniel Silva Moraes, Guilherme Henrique Mendes Ribeiro, Rodrigo Moreira da Silva, Norberto Peporine Lopes, André Luiz Sena Guimarães, Alfredo Maurício Batista de Paula, Sérgio Henrique Sousa Santos
BACKGROUND: Lychnophora ericoides Mart, also known as the Brazilian arnica or fake arnica, belongs to the Asteraceae family. Leaves and roots are used in alcoholic and hydroalcoholic preparations for the treatment of wounds, inflammation, and pain. PURPOSE: The present study aimed to investigate the effects of L. ericoides ethanolic extract (EELE) on cutaneous wound healing and the mechanisms of action involved. METHODS: A total of 72 C57BL/6 mice were randomly divided into four groups of six animals each...
July 28, 2023: Phytomedicine
https://read.qxmd.com/read/37538236/application-of-menstrual-blood-derived-stromal-stem-cells-exert-greater-regenerative-potency-than-fibroblasts-keratinocytes-in-chronic-wounds-of-diabetic-mice
#13
JOURNAL ARTICLE
Ebrahim Mirzadegan, Hannaneh Golshahi, Zahra Saffarian, Haleh Edalatkhah, Maryam Darzi, Somayeh Khorasani, Kioomars Saliminejad, Somaieh Kazemnejad
BACKGROUND: In this study we differentially showed the effects of cell-seeded bilayer scaffold wound dressing in accelerating healing process in diabetic ulcers that still remains as a major clinical challenge. The aim of the study was to compare immunomodulatory and angiogenic activity, and regenerative effect differences between Menstrual blood-derived Stem Cells (MenSCs) and foreskin-derived keratinocytes/fibroblasts. METHODS: The streptozotocin-induced diabetic mice model was developed in male C57/BL6 mice...
2023: Avicenna Journal of Medical Biotechnology
https://read.qxmd.com/read/37366253/the-adipose-derived-stem-cell-peptide-adscp2-alleviates-hypertrophic-scar-fibrosis-via-binding-with-pyruvate-carboxylase-and-remodeling-the-metabolic-landscape
#14
JOURNAL ARTICLE
Jingyun Li, Yiliang Yin, Jijun Zou, Enyuan Zhang, Qian Li, Ling Chen, Jun Li
AIM: The purpose of this study was to investigate the function and mechanism of a novel peptide derived from adipose-derived stem cell-conditioned medium (ADSC-CM). METHODS: Mass spectrometry was applied to identify expressed peptides in ADSC-CM obtained at different time points. The cell counting kit-8 assay and quantitative reverse transcription polymerase chain reactions were performed to screen the functional peptides contained within ADSC-CM. RNA-seq, western blot, a back skin excisional model of BALB/c mice, the peptide pull-down assay, rescue experiments, untargeted metabolomics, and mixOmics analysis were performed to thoroughly understand the functional mechanism of selected peptide...
August 2023: Acta Physiologica
https://read.qxmd.com/read/37209062/protecting-human-amnion-and-chorion-matrices-hacm-during-processing-performance-enhancement-in-a-diabetic-mouse-model-and-human-co-culture-system
#15
JOURNAL ARTICLE
Mitchell Corey Sanders, Swathi Balaji, W Blake Martin, Nikole Siegmund, Lindsay Poland, Mia Sanders Hanna, Da Wei, Hanna Kaliada, Sara Littlejohn, Timothy Ganey
Recent evidence suggests that protecting human amnion and chorion matrices (HACM)1 during processing enhances the performance of HACM for wound repair and tissue regeneration. We utilized a diabetic (db/db) delayed wound healing mouse model. Treatment of db/db full thickness excisional wounds with HACM, processed with a polyampholyte preservative accentuated the proliferative phase of wound healing that decreased the time necessary to heal wounds. Polyampholyte protection improved the preservation of growth factors and cytokines during room temperature storage following E-beam sterilization and improved its function in wound healing applications...
May 20, 2023: Wound Repair and Regeneration
https://read.qxmd.com/read/37193239/-in-vivo-efficiency-of-the-produced-recombinant-lysostaphin-antimicrobial-peptide-in-treatment-of-methicillin-resistant-staphylococcus-aureus-mrsa-skin-infection-in-a-mouse-model
#16
JOURNAL ARTICLE
Shabnam Sadoogh Abbasian, Ehsanollah Ghaznavi-Rad, Samira Sadelaji, Hamid Abtahi
BACKGROUND AND OBJECTIVES: Staphylococcus simulans secretes an antimicrobial compound called lysostaphin, which has bactericidal properties. It destroys staphylococci through the hydrolysis of peptidoglycan in the cell wall. Therefore, this unique property indicates the high ability of lysostaphin in the treatment of staphylococcal infections and is considered as an anti-staphylococcal agent. MATERIALS AND METHODS: Escherichia coli BL21 (DE3) competent cells were transformed with pET32a-lysostaphin clone and induced by isopropyl-β-D-thio-galactoside (IPTG)...
April 2023: Iranian Journal of Microbiology
https://read.qxmd.com/read/37165726/egfr-marks-a-subpopulation-of-dermal-mesenchymal-cells-highly-expressing-igf1-which-enhances-hair-follicle-regeneration
#17
JOURNAL ARTICLE
Min Chen, Zaoxu Xu, Yu Chen, Qingyang Yang, Ruiqing Lu, Yankai Dong, Xiaosong Li, Jundong Xie, Ren-He Xu, Haidong Jia, Yan Kang, Yaojiong Wu
The skin harbours transcriptionally and functionally heterogeneous mesenchymal cells that participate in various physiological activities by secreting biochemical cues. In this study, we aimed to identify a new subpopulation of dermal mesenchymal cells that enhance hair follicle regeneration through a paracrine mechanism. Integrated single-cell RNA sequencing (scRNA-seq) data analysis revealed epidermal growth factor receptor (EGFR) as a marker of distinct fibroblast subpopulation in the neonatal murine dermis...
May 10, 2023: Journal of Cellular and Molecular Medicine
https://read.qxmd.com/read/37142683/metrnl-deficiency-retards-skin-wound-healing-in-mice-by-inhibiting-akt-enos-signaling-and-angiogenesis
#18
JOURNAL ARTICLE
Tian-Ying Xu, Sheng-Li Qing, Jing-Xin Zhao, Jie Song, Zhu-Wei Miao, Jia-Xin Li, Feng-Yan Yang, Huan-Yu Zhao, Si-Li Zheng, Zhi-Yong Li, Shu-Na Wang, Chao-Yu Miao
Meteorin-like (Metrnl) is a novel secreted protein with various biological activities. In this study, we investigated whether and how Metrnl regulated skin wound healing in mice. Global Metrnl gene knockout mice (Metrnl-/- ) and endothelial cell-specific Metrnl gene knockout mice (EC-Metrnl-/- ) were generated. Eight-mm-diameter full-thickness excisional wound was made on the dorsum of each mouse. The skin wounds were photographed and analyzed. In C57BL/6 mice, we observed that Metrnl expression levels were markedly increased in skin wound tissues...
May 4, 2023: Acta Pharmacologica Sinica
https://read.qxmd.com/read/37046252/lean-adipose-tissue-macrophage-derived-exosome-confers-immunoregulation-to-improve-wound-healing-in-diabetes
#19
JOURNAL ARTICLE
Wenzheng Xia, Yunhan Liu, Xingyu Jiang, Minxiong Li, Shengwu Zheng, Zewei Zhang, Xin Huang, Shenying Luo, Yimin Khoong, Meng Hou, Tao Zan
Chronic non-healing wounds, a prevalent complication of diabetes, are associated with increased mortality in diabetic patients. Excessive accumulation of M1 macrophages in diabetic wounds promotes inflammation and results in dysregulated tissue repair. Adipose tissue macrophages (ATMs) derived from healthy lean donors have the ability to improve glucose tolerance and insulin sensitivity, as well as modulate inflammation. MicroRNAs (miRs), which can be packaged into exosomes (Exos) and secreted from cells, serve as essential regulators of macrophage polarization...
April 12, 2023: Journal of Nanobiotechnology
https://read.qxmd.com/read/36999070/nitric-oxide-releasing-gel-accelerates-healing-in-a-diabetic-murine-splinted-excisional-wound-model
#20
JOURNAL ARTICLE
Dharshan Sivaraj, Chikage Noishiki, Nina Kosaric, Harriet Kiwanuka, Hudson C Kussie, Dominic Henn, Katharina S Fischer, Artem A Trotsyuk, Autumn H Greco, Britta A Kuehlmann, Filiberto Quintero, Melissa C Leeolou, Maia B Granoski, Andrew C Hostler, William W Hahn, Michael Januszyk, Ferid Murad, Kellen Chen, Geoffrey C Gurtner
INTRODUCTION: According to the American Diabetes Association (ADA), 9-12 million patients suffer from chronic ulceration each year, costing the healthcare system over USD $25 billion annually. There is a significant unmet need for new and efficacious therapies to accelerate closure of non-healing wounds. Nitric Oxide (NO) levels typically increase rapidly after skin injury in the inflammatory phase and gradually diminish as wound healing progresses. The effect of increased NO concentration on promoting re-epithelization and wound closure has yet to be described in the context of diabetic wound healing...
2023: Frontiers in Medicine
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