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Forensic Genetic

Ying Huang, Yunmei Zhang, Weitao Wang, Kai Zhao, Guangxing Li, Bingying Xu
The Lisu is an ethnic minority living in highlands or mountain valleys in the northern region of the Indo-China Peninsula. The paper presents the frequency distribution of allele and statistical genetic parameters of forensic relevance for 15 autosomal STR loci found in the AmpFℓSTR® Identifiler® PCR Amplification Kit among a population sample constituted by 1854 non-related Lisu minority individuals residing in the southwestern region of China. The genetic relationships between Lisu population and 14 related populations were assessed...
February 19, 2019: International Journal of Legal Medicine
Pengyu Chen, Guanglin He, Hao Xing, Hongyan Gao, Menge Wang, Min Zhao, Li Luo, Jian Wu, Jian Yu, Yanyan Han
BACKGROUND: Investigation of haplotype/allele frequency data of Y-STR loci in ethnically diverse populations is essential for forensic reference database construction and genetic application. However, the population genetic characteristics of the Chinese Miao minority from Guizhou Province remain uncharacterised. AIM: To assess forensic characteristics for 23 Y-Chromosomal STR loci in Guizhou Miao and explore population genetic relationships with geographically neighbouring populations...
February 19, 2019: Annals of Human Biology
Thi Dao Dinh, Jacob Njaramba Ngatia, Liang Yu Cui, Yue Ma, Thomas D Dhamer, Yan Chun Xu
Species identification is fundamental to wildlife forensic practice. The desirability of molecular genetic methods is increasing rapidly. The sequence of a marker, rather than its particular diagnostic nucleotides, provides greater safety through comparisons between intra- and inter-specific pairwise genetic distances. However, it has not been well described how reliability of species assignment is influenced by distance computing methods and reference sample sizes. In this study, the influences were tested using 12 species from 4 genera of passerine birds and the sequences of partial Cytochrome b (Cyt b) and Cytochrome Oxidase subunit I (COI) genes...
February 13, 2019: Forensic Science International. Genetics
Eduardo Avila, Aline Brugnera Felkl, Pietra Graebin, Cláudia Paiva Nunes, Clarice Sampaio Alho
Use of Massive Parallel Sequencing (MPS) techniques has been investigated by forensic community aiming introduction of such methods in routine forensic casework analyses. Interesting features presented by MPS include high-throughput, ability to simultaneous genotyping of significant number of samples and forensic markers, workflow automation, among others. Emergence of single nucleotide polymorphism (SNP) as forensic relevant markers was facilitated in this process, since concurrent typing of larger marker sets is necessary for obtaining same levels of individual discrimination provided by other marker categories...
February 13, 2019: Forensic Science International. Genetics
Tianshan Guan, Xuheng Song, Cheng Xiao, Huilin Sun, Xiaoying Yang, Chao Liu, Ling Chen
Genetic polymorphisms of 23 Y-chromosomal short tandem repeats (Y-STR) were investigated by PowerPlex® Y23 System in 328 unrelated male participants from Jieyang, Guangdong Province of China. A total of 293 haplotypes were obtained, and the haplotype diversity (HD) and discrimination capacity (DC) were 0.9991 and 0.8933, respectively. By comparing Jieyang population with nine other populations, Jieyang Han showed close genetic relationships with southern China-related Han populations. In conclusion, our study increased the Y-chromosome haplotype reference database and could provide useful information for forensic investigation and population genetics...
February 18, 2019: International Journal of Legal Medicine
Ling Chen, Weian Du, Weibin Wu, Anlu Yu, Xiyong Pan, Peipei Feng, Chunlei Feng, Chen Li, Lingdan Xu, Changhui Liu, Chao Liu
In this study, a multiplex amplification system including 47 autosomal InDels, 2 Y-chromosome InDels, and the sex-determining marker (Amelogenin) was developed with six fluorescent dyes labeling. These InDels were selected from the previous study based on a series of criteria (0.3 < MAF < 0.5, HET > 0.4, etc). The system was designated the AGCU InDel 50 kit and was validated in a series of studies, including a degradation study; tests for sensitivity, species specificity, reproducibility, stability, applicability to case samples, balance of peak height, and PCR conditions; and a population study...
February 12, 2019: Forensic Science International. Genetics
Mikkel Meyer Andersen, James Curran, Jacob de Zoete, Duncan Taylor, John Buckleton
No abstract text is available yet for this article.
January 22, 2019: Forensic Science International. Genetics
Vishakha Sharma, Krupa Jani, Pavan Khosla, Erin Butler, Donald Siegel, Elisa Wurmbach
This study examined 266 individuals from various populations including African American, East Asian, South Asian, European, and mixed populations to evaluate the ForenSeq™ Signature Prep Kit Primer Mix B. Focus was placed on phenotypic and biogeographical ancestry predictions by Illumina's Universal Analysis Software (UAS). These outcomes were compared to those obtained through web-tools developed at the Erasmus Medical Center (EMC) and available from the Forensic Resource/Reference on Genetics-knowledge base (FROG-kb), as well as to eye color predictions by the 8-plex system...
February 15, 2019: Electrophoresis
Emuejevoke T Toye, Guido van Marle, Wendy Hutchins, Olayinka Abgabiaje, Joy Okpuzor
Background: Sickle cell anaemia (SCA) is a hereditary blood disorder caused by a single mutation in the haemoglobin gene. The disease burden of SCA is highest in Nigeria. The allele specific polymerase chain reaction (ASPCR) method is applicable for the direct detection of known single nucleotide polymorphisms (SNPs). Objective: To investigate the use of the single tube ASPCR as an accurate and affordable method for SCA screening in Nigeria. Methods: DNA was extracted from study subjects with normal haemoglobin, HbAA (20), sickle cell anaemia, HbSS (20) and carriers, HbAS (1)...
December 2018: African Health Sciences
Yaoqi Liao, Ling Chen, Runze Huang, Weibin Wu, Dayu Liu, Huilin Sun
Here we studied the genetic polymorphism and evolutionary differentiation of the Guangdong Liannan Yao population based on 15 autosomal STR loci and 19 Y chromosomal STR loci. The blood card DNA of 302 unrelated individuals from the Yao Autonomous County of Liannan was directly amplified using an Expressmarker 16 + 19Y kit and genotyped using a 3500XL Genetic Analyzer. For the autosomal STR loci, the CPD value was over 0.999 999 999 999, while the CPE value was over 0.9999. The population comparison revealed a closer relationship between the Liannan Yao population and the She ethnic population than other reported Chinese populations...
February 14, 2019: Scientific Reports
Qinrui Yang, Baonian Liu, Chengchen Shao, Yuxiang Zhou, Yining Yao, Yuyin Pan, Kuan Sun, Hongmei Xu, Chengtao Li, Ting Wei, Yueqin Zhou, Qiqun Tang, Jianhui Xie
BACKGROUND: An STR locus with tri-allelic pattern is occasionally observed in routine forensic casework. The extra copy of TPOX locus with tri-allelic pattern in populations has been assumed to be inserted into an X chromosome, which took place forth before the Bantu expansion in Africa. Nonetheless, the exact location of the duplication and the form of rearrangement in the human genome has not been clarified yet. RESULTS: In this study, we investigated the extra copy of type 2 tri-allelic pattern at TPOX in various populations...
February 14, 2019: BMC Genetics
Shivani Dixit, Pankaj Shrivastava, R K Kumawat, Kamlesh Kaitholia, Hirak Ranjan Dash, Harsh Sharma, Gyaneshwer Choubey
Performance of PowerPlex Fusion 6C kit (PP F6C) was assessed in 374 unrelated individuals belonging to Madhya Pradesh, an Indian state. The study evaluated the forensic parameters for the loci included in PP F6C Multiplex System. The combined discrimination power (CPD) and combined exclusion power (CPE) were 1 and 0.999999995, respectively, for all 23 autosomal STR loci. SE33 showed the greatest power of discrimination (0.990) in the studied population, whereas TPOX showed the lowest (0.843). The availability of three Y-STR loci in the Multiplex System is suitable for assessing male contribution and amelogenin deletion in a single Multiplex PCR simultaneously...
February 14, 2019: International Journal of Legal Medicine
Qiong Lan, Chunmei Shen, Xiaoye Jin, Yuxin Guo, Tong Xie, Chong Chen, Wei Cui, Yating Fang, Guang Yang, Bofeng Zhu
In the forensic field, ancestry-informative markers showing remarkable allele frequency discrepancies can be useful in deducing the likely ancestral origin of a person or estimating the ancestry component proportions of admixed populations or individuals. Diallelic SNPs are genetic markers commonly used for ancestry inference, but the genotyping methods of SNPs fail to fulfil the demands of cost-effectiveness and simplicity of experimental manipulation. To overcome the limitations, a 39 ancestry-informative InDels multiplex panel was developed in the present study to perform ancestry assignment of individuals from three distinct biogeographic regions (African, European, East Asian)...
February 13, 2019: Electrophoresis
Henrik Green, Andreas Tillmar, Gisela Pettersson, Kerstin Montelius
Filter papers have been used for many years in different applications of molecular biology and have been proven to be a stable way to store DNA waiting to be analyzed. Sampling of DNA on FTA (Flinders Technology Associates) cards is convenient and cost effective compared to alternative approaches involving DNA extractions and storage of DNA extracts. FTA cards are analyzed at many forensic laboratories, and the way to perform direct genetic profiling on buccal swab cards has developed into an almost industrial process...
February 12, 2019: International Journal of Legal Medicine
Luyao Li, Yi Ye, Feng Song, Zheng Wang, Yiping Hou
Insertion/deletion analysis can serve as a promising and useful supporting tool in forensic research. The Qiagen Investigator® DIPplex Kit contained 30 well-chosen autosomal InDels was targeted to reveal the population genetic variation. In the present study, 10 Tibetan populations residing in different geographic areas of China were recruited and genotyped by Investigator® DIPplex Kit. Allele frequencies and forensic parameters were determined. No significant departures from Hard-Weinberg equilibrium (HWE) in all loci/populations after Bonferroni correction...
February 5, 2019: Forensic Science International. Genetics
Martin Zieger, Silvia Utz
Comparatively little knowledge is available about the genetic structure of the Swiss population. Missing allele frequency data for some markers frequently used in forensics and paternity / kinship testing is just one practical aspect of this lack of genetic data. Therefore, in an attempt to fill this gap, we established a biobank of 1198 Swiss blood samples, systematically collected throughout the whole country. For the first time, this collection contains a population sample from the southernmost Italian speaking canton of Ticino...
February 5, 2019: Forensic Science International. Genetics
Clémence Hollard, Lionel Ausset, Yann Chantrel, Sandrine Jullien, Maéva Clot, Magalie Faivre, Élodie Suzanne, Laurent Pène, François-Xavier Laurent
Massively parallel sequencing (MPS) applications in forensic science highlight the advantages of this technique compared to capillary electrophoresis (CE). The multiplexing of a wide range of genetic markers and access to the full amplicon sequence, allowing the detection of isoalleles, make it a very promising tool which could be applied to the most challenging casework DNA samples. However, the complexity of the manual library preparation protocol, potential DNA contamination and sample tracking issues are the main reasons why forensic scientists still hesitate to implement MPS analytical workflows in their laboratory...
January 31, 2019: Forensic Science International. Genetics
Mariela Caputo, A Sala, D Corach
The Y chromosome behaves as a single locus. Its genetic information is useful in forensic casework, deficiency kinship testing, and population genetics studies. Continuous increases of loci number within commercial kits forced modification of worldwide reference databases. In Pan American countries, like Argentina, diverse parental ethnic groups contributed to the extant admixed urban populations. We report 509 additional haplotypes of 23 Y-STRs from donors inhabiting urban areas of six Argentinean provinces: Buenos Aires, Santiago del Estero, Santa Cruz, Rio Negro, Santa Fe, and Formosa...
February 9, 2019: International Journal of Legal Medicine
Katelyn E Mason, Phillip H Paul, Fanny Chu, Deon S Anex, Bradley R Hart
Shed human hair (lacking root nuclear DNA) frequently contributes important information to forensic investigations involving human identification. Detection of genetic variation observed in amino acid sequences of hair proteins provides a new suite of identity markers that augment microscopic hair analysis and mitochondrial DNA sequencing. In this study, a new method that completely dissolves single hairs using a combination of heat, ultrasonication, and surfactants was developed. Dissolved proteins were digested and genetically variant peptide (GVP) profiles were obtained for single hairs (25 mm) via high-resolution nanoflow liquid chromatography-based mass spectrometry and a novel exome-driven bioinformatic approach...
February 8, 2019: Journal of Forensic Sciences
Jillian Conte, Margret J Potoczniak, Courtney Mower, Shanan S Tobe
A framework for the development and validation of a qPCR assay for species identification and DNA quantification for conservation and forensic purposes is presented. Elephants are commonly poached for their ivory tusks, which is the primary driving force behind their endangered status. In addition to poaching and trade, habitat loss due to logging and mining has also resulted in loss of elephants. Crimes against animals can be deterred and/or further prosecution sought through testing with forensic genetic techniques...
February 5, 2019: Molecular Biology Reports
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